• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.158-162
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• 2005

Enterobacteria, named ${\lambda}-bacteria$ isolated from fusiform fish, have strong anti-angiogenesis effect. ${\lambda}-28$ species bore higher anti-angiogenesis effect. Cultured liquid was performed salting out, dialysed and freezed dried. This sample was executed size exclusion chromatography with fraction collector. Anti-angiogenesis, cytotoxicity, and SDS-PAGE were carried out with fraction number. ${\lambda}-28$ species was lower toxicity against HUVECs and effective band was conformed with SDS-PAGE.

• Korean journal of food and cookery science
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• v.12 no.3
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• pp.331-338
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• 1996

The main aim of this study was to develop carrot soups with fishery products, which is improved from traditional gruel of Cheju island. For this the optimal procedure and ingredient mixing ratio for making basic carrot soup was determined through the instrumental measurement, the sensory evaluation and the nutrient analysis, and then the carrot-fishery soups were prepared by mixing the fishery products from Cheju island. The results were as follows: The optimal procedure for making basic carrot soup was to saute the sliced carrots and soaked rices with the sesame oil for 5 min, add the water, bring to a boil for 6 min, simmer for 15 min, then puree the soup. The optimal ingredient mixing ratio in the basic carrot soup was carrot 300 g: rice 45 g: water 900 $m\ell$: sesame oil 15 $m\ell$: salt 5 g. For preparing carrot-fishery soups were the optimal fishery products mixing ratio i) 30% tile fish or crab, ii) prawn, abalone, top shell, or ear shell 20%, iii) sea urchin 15%, iv) fusiform or gulf weed 5％. The order of sensory evaluation scores, on a 5-point scale, were sea urchin, crab > top shell, tile fish > abalone, ear shell > prawn > coral fish > fusiform, gulf weed. Nutrient composition analysis showed that vitamin A was 5 times higher in carrot soup than in pumpkin soup. Sensory evaluation scores show that carrot soup was prepared to pumpkin soup. A portion (200 g) of the soup would provide 144% of the recommended daily allowance of vitamin A. The results of this work indicate that an acceptable carrot-fishery soups of better nutritional and sensory values than pumpkin soup can be prepared.

• Journal of Marine Bioscience and Biotechnology
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• v.1 no.3
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• pp.156-162
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• 2006

The halophilic enterobacteria, Enterobacteria cancerogenus, was isolated from the intestines of the fusiform fish (Trachurus japonicus) to yield a protein-like material termed PLM-f74. PLM-f74 was characterized by strong inhibition ratios to angiogenesis (82.8% at the concentration of $18.5{\mu}g/mL$) and elevated antioxidative capacities with low toxicity. The PLM-f74 is a glycoprotein comprised of saccharides and amino acids. PLM-f74 inhibited non-activated U937 monocytic cell adhesion to HUVECs activated with IL-$1{\beta}$ by 78.0%, and the adherence of U937 cells treated with the PLM-f74 and stimulated with IL-$1{\beta}$ to unstimulated HUVECs decreased by 102%. When both cell types were pretreated with PLM-f74, the adhesion of U937 cells to IL-$1{\beta}$ stimulated HUVECs was completely suppressed by 121% at a concentration of 18.5 ug/mL. PLM-f74 blocked signal pathways from VEGFR2, PI3K, ${\beta}$-catenin and VE-cadherin to NF-kB based on western bolt analysis. And also inhibited IL-1-stimulated HUVEC expression of the adhesion molecules, ICAM-1 by 40%, VCAM-1 by 60%, and E-selectin by 70% at the same concentration noted above. New anti-angiogenic and anti-cell adhesion materials showing elevated antioxidative capacities and non-toxicity may be expected from these results.

• Journal of Microbiology and Biotechnology
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• v.16 no.10
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• pp.1544-1553
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• 2006

The halophilic enterobacteria, Enterobacteria cancerogenus, was isolated from the intestines of the fusiform fish (Trachurus japonicus) to yield a protein-like material termed PLM-f74. PLM-f74 was characterized by strong inhibition ratios to angiogenesis (82.8% at the concentration of $18.5{\mu}g/ml$) and elevated antioxidative capacities with low toxicity. The PLM-f74 is a glycoprotein comprised of saccharides and amino acids. PLM-f74 inhibited cell adhesion that non-activated U937 monocytic cell adhesion to HUVECs activated with $IL-1{\beta}$ by 78.0%, and the adherence of U937 cells treated with the PLM-f74 and stimulated with $IL-1{\beta}$ to unstimulated HUVECs decreased by 102%. When both cell types were pretreated with PLM-f74, the adhesion of U937 cells to $IL-1{\beta}$-stimulated HUVECs was completely suppressed by 121% at a concentration of $18.5{\mu}g/ml$. PLM-f74 blocked signal pathways from VEGFR2, PI3K, ${\beta}$-catenin, and VE-cadherin to NF-kB, based on western bolt analysis. It also inhibited IL-l-stimulated HUVEC expression of the adhesion molecules, ICAM-l by 40%, VCAM-l by 60%, and E-selectin by 70% at the same concentration noted above. New anti-angiogenic and anti-cell adhesion materials showing elevated antioxidative capacities, and non-toxicity may be expected from these results.

• Journal of Plant Biology
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• v.26 no.1
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• pp.7-16
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• 1983

Internodal vessel elements are studied in 20 genera, 75 species and 33 cultivars of Hibiscus rosa-sinensis belong to the Malvaceae. The minimum and maximum length and diameter range from 79~466 and 14~88 ${\mu}{\textrm}{m}$, respectively. The vessel elements are either cylindrical, fusiform, conical, clavate, oval, column-, drum-, fish and cup-like or erratic. Perforation plates are exclusively simple in all the species investigated, except occasionally scalariform in varieties of Hibiscus rosa-sinensis. In most of the species studied vessel elements have predominantly two perforation plates or occasionally one and three. Adjacen side wall thickening is commonly simple and border pitted, mixed, scalariform, helical and reticulate. The vessel elements vary in their size, shape, number and inclination of perforation plates and adjacent wall thickening.

• Journal of fish pathology
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• v.9 no.1
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• pp.21-31
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• 1996

Concerned to the Ichthyophthiriasis of aquacultural fishes, the developmental features of Ichthyophthirius multifiliis were studied in cultured Korean catfish, Silurus asotus, and rainbow trout, Oncorhynchus mykiss. In the morphological observation, the parasite developed on two kinds of parasitic and non-parasitic phases with 6 developmental stages termed phoront, trophont, protomont, tomont, tomite, and therone. The $60{\times}40-100{\times}70{\mu}m$ fusiform or spherical phoront for the invading stage has 34-38 meridional kineties and begins to develope buccal apparatus. The 80-$800{\mu}m$ spherical or amoeboid trophont for the vegetative stage has a horseshoe shape macronucleus, a inconspicuous cytostome and developmental contractile vacuoles. The 200-$800{\mu}m$ spherical protomont for the encysting stage has a inconspicuous macronucleus, abundant contractile vacuoles; and a fine gelatinous exocyst is exuded, the baccal apparatus begins to resorb. The tomont for the encysted dividing stage has a thick cyst wall, and the buccal apparatus is resorbed completely. A small 35-$50{\mu}m$ spherical tomite for each daughter cell has a cytostome end the conspicuous oral apparatus. The $25{\times}20-60{\times}40{\mu}m$ fusiform theront for the infective stage possesses a perforatorium in the anterior end, a cytostome in the mid-point respectively and has 34-38 meridional kineties. In the experiments of the reproductive, the excysted time is related to water temperature. Tomitogenesis takes 10-14 hours at $28^{\circ}C$, 12-15 hours at $26^{\circ}C$, 16-18 hours at $22^{\circ}C$, 24-28 hour at $18^{\circ}C$, 26-51 hours at $14^{\circ}C$, and 129 hours at $9^{\circ}C$ respectively.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.406-411
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• 2005

Fermented materials with enterobacteria isolated from fusiform fish, have strong anti-angiogenesis effect and anti-cell adhesion effect. PLM-f74 got from 74th fraction of size exclusion chromatography from fermented material, showed strong anti-cell adhesion effect between HUVECs and U937 monocytic cell. Adhesion of U937 cell to HUVEC stimulated with IL-1b was clearly inhibited by PLM-f74 in a dose-dependent manner by 12.1, 21.2, 50.9, and 78.2%, when U937 cells treated with each of the PLM-f74 and stimulated with PMA (100 mg/L) was added onto untreated and unstimulated HUVECs, adhesion was observed by 15.8, 31.9, 70.8, and 102%, when both cell types were pretreated with PLM-f74, the adhesion was prominently decreased by 83.7, 99.2, 110, and 120.8%, with 0.74, 3.7, 7.4, and 18.5ug/mL of PLM-f74, respectively. PLM-f74, also, reduced IL-1-stimulated HUVEC expression of adhesion molecules, VCAM-1, ICAM-1, and E-selectin dose-dependently by ELISA method.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.335-339
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• 2005

Enterobacteria, named ${\lambda}-bacteria$ isolated from fusiform fish bearing higher antioxidative capacity with ORP values, ${\lambda}-28$ strain bore higher anti-angiogenesis effect than other ${\lambda}-species$. Optimum growth condition of ${\lambda}-28$ bacterium was $25^{\circ}C$, neutral pH, Glc as a C-source, ammonium chloride as a N-source, and not effected with organic N-source.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.59 no.3
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• pp.189-195
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• 2023

This study quantitatively evaluated size selectivity for three netting shapes (T0; regular, T45, T90) and hanging ratio (35%, 70%) of T0 netting used for trawl codend. The size selectivity experiment was performed in a tank using a cube experimental model with a length of 50 cm on one side and 389 experimental individuals, jack mackerel (Trachurus japonicus). In the selectivity analysis, a selectivity curve was created based on the selection ratio using a logistic function, and the 25%, 50%, and 70% selection length and selection range (SR) were obtained. The T0 netting was 19.54 cm when the 50% selective length, which is a selectivity evaluation index, had a hanging ratio of 35%, a selection range of 0.51 cm, and 22.70 cm and 3.08 cm for the hanging ratio of 70%. The T45 netting was 24.34 cm and 2.13 cm, and the T90 netting was 23.51 cm and 2.84 cm. The results of the T45 netting and the T90 netting are similar, and the 50% selection length and selection range were relatively larger than the T0 netting. There was a significant difference in the correlation between the circumference of the inner circle of the mesh by the shape of the netting and the body girth of the experimental individual (Pearson test, r = 0.86, p < 0.05). There was no significant difference in the correlation between the selection ratio by the T0 netting, T45 netting, and T90 netting with a 70% hanging ratio (one-way ANOVA, p > 0.05). The results of this study showed that selectivity such as T45 netting and T90 netting appeared when the hanging ratio, which maximizes the area of T0 netting, was maintained at 70%.

• Korean Journal of Ichthyology
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• v.30 no.2
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• pp.75-83
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• 2018

This study is to observe species identification and early life history of Korean endemic species of Tanakia latimarginata and to use it as a basis for taxonomic studies. As a result of morphological identification, a dark band appeared at the margin of the anal fin, and the ovipositor color of the female was light orange. The shape of the egg was fusiform and sticky. The egg size (long${\times}$short diameter) averaged $4.41{\times}1.44mm$. The incubation time was 126 hours after the fertilization at an average water temperature of $21.0^{\circ}C$. Immediately after hatching, the larvae had egg yolk at an average total length of $5.91{\pm}0.18mm$ (n=5). At 18 days after hatching, the trunk fur was developed in the caudal fin with an average total length of $8.02{\pm}0.08mm$ (n=5). At 41 days after hatching, the larvae absorbed egg yolk at an average total length of $8.70{\pm}0.23mm$ (n=5). At 80 days after hatching, the average length of the fins was $12.6{\pm}0.28mm$ (n=5). The number of fin of the dorsal fin was iii.8, the anal fin iii.9~10, the caudal fin 19, lateral line scales 32~35 were similar to their brood stork.