• Journal of Microbiology and Biotechnology
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• v.6 no.3
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• pp.202-208
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• 1996

In order to investigate fermenting conditions and the microorganisms necessary for factory production of traditional Korean soy sauce, we manufactured soy sauce made by Bacillus species SSA3-2M1 and fused ST723-F31 with aeration (1/30 vvm, 113 vvm and 2/3 vvm) at $30^{\circ}C$ for 40 days. This method was chosen to investigate the changes of dissolved oxygen, pH, cell number, flavor and the taste components during fermentation. When air was supplied (2/3 vvm) to the fermentor during fermentation, the flavor of the soy sauce and the composition of taste components (free amino acids, free sugars and organic acids) were similar to that of traditional Korean soy sauce after 22 days. The results of our experiments indicates that the mass production of traditional Korean soy sauce is possible using Bacillus species SSA3-2M1 and fused ST723-F31 given sufficient aeration.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.5 no.7
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• pp.1354-1360
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• 2001

Calculated velocity distribution was used to visual information by image that is obtained from camera. The visual velocity of object that is obtained from this visual information were fused and experimented. That is, need motion of eye that motion of head that happen by external disturbance or move of camera itself to get stable image in environment that receive external disturbance can be compensated. In this treatise, algorithm that control gaze which vision and sense of equilibrium are fused in environment with external disturbance proposed, and thing that compare with that it controls gaze only that control gaze which vision and sense of equilibrium are fused in the experiment result and position deflection is few confirmed. This was because action of camera prop is effect that record conclusion error of the speed because the appearance speed is decreased being compensated by angular velocity sensor.

• Journal of Microbiology and Biotechnology
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• v.8 no.1
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• pp.34-41
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• 1998

A method was developed for the controlled expression of an antimicrobial peptide magainin in Escherichia coli. A series of concatemeric magainin genes was constructed with a gene amplification vector, and fused to the 3'end of malE gene encoding the affinity ligand, E. coli maltose-binding protein (MBP). The construct directed the synthesis of the fusion protein with the magainin polypeptide fused to the C-terminus of MBP. The fusion protein was expressed in a tightly regulatable expression system which was under the control of an invertible promoter. The MBP-fused magainin monomer was expressed efficiently. However, the expression level of the MBP-fused magainin in E. coli decreased with the increasing size of multimers possibly because of the transcription and translation inhibition by the multimeric peptides. After purification using an amylose affinity column, the fusion protein was digested by factor Xa at a specific cleavage site between the monomers. The recombinant magainin had an antimicrobial activity identical to that of synthetic magainin. This experiment shows that a biologically active, antimicrobial peptide magainin can be produced by fusing to MBP, along with a promoter inversion vector system.

• Korean Journal of Veterinary Research
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• v.43 no.4
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• pp.521-524
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• 2003

The sacrum fused with first caudal vertebra and ilium of a male German shepherd were observed macroscopically. The sacrum and first caudal vertebra were fused, however caudal articular process of the sacrum separated from the cranial articular process of the first caudal vertebra. On the other hand, partial fusion were also observed between the sacrum and ilium. The sacrum and ilium were fused from the cranioventral part of the sacropelvic surface in the wing of ilium to the lateral part of the right cranial articular surface in the sacrum.

• The Journal of Korean Academy of Prosthodontics
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• v.39 no.6
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• pp.633-640
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• 2001

Statement of problem. Relatively low success rate of root analogue implant system was supposed to be due to the time duration between extraction and implant installation. The use of three-dimensional computer tomography and the reconstruction of objects using rapid prototyping methods would be helpful to shorten this time. Purpose. This aim of this study was to evaluate the application possibility of the 3-dimensional computer tomography and the rapid prototyping to root analogue implants. Material and methods. Ten single rooted teeth were prepared. Width and height of the teeth were measured by the marking points. This was followed by CT scanning, data conversion and rapid prototyping model fabrication. 2 methods were used; fused deposition modelling and stereolithography. Same width and height of this models were measured and compared to the original tooth. Results. Fused deposition modelling showed an enlarged width and reduced height. The stereolithography showed more exact data compared with the fused deposition modelling. Smaller standard deviation were recorded in the stereolithographic method. Overall width error from tooth to rapid prototyping was 7.15% in fused deposition modelling and 0.2% in stereolithography. Overall height showed the tendency of reducing dimensions. Conclusion. From the results of this study, stereolithography seems to be very predictable method of fabricating root analogue implant.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.18-21
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• 2003

Fused Deposition Modeling (FDM) parts are made by piling up thin layers that cause the stair stepping effect at the surface of FDM parts. This effect brings about poor surface roughness of the part and requires additional post machining such as manual finishing that is detrimental to the part geometry and time consuming. Determining optimal build orientation for FDM parts can be one solution to minimize the post machining. However, by using the CAD model, calculating the optimal build orientation is impractical due to heavy computing process. In order to calculate the optimal build orientation with high speed. the surface roughness model based on measured data and interpolation is newly developed in this research. Also. the genetic algorithm (GA) is applied for acquiring reliable solution. Finally, It is verified from the test that the presented approach is very efficient for reducing the additional post machining process fer FDM parts.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.21 no.5
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• pp.761-766
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• 2012

Abrasive fluid jet polishing processes have been used for the polishing of optical surfaces with complex shapes. However, unstable and unpredictable polishing spots can be generated due to the fundamental property of an abrasive fluid jet that it begins to lose its coherence as the jet exits a nozzle. To solve such problems, MR fluid jet polishing has been suggested using a mixture of abrasives and MR fluid whose flow properties can be readily changed according to imposed magnetic field intensity. The MR fluid jet can be stabilized by imposed magnetic fields, thus it can remain collimated and coherent before it impinges upon the workpiece surface. In this study, MR fluid jet polishing characteristics of fused silica glass were investigated according to injection time and magnetic field intensity variations. Material removal rates and 3D profiles of the generated polishing spots were investigated. From the results, it can be confirmed that the developed MR fluid polishing system can be applied for stable and predictable precise polishing of optical parts.

• Design & Manufacturing
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• v.14 no.1
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• pp.36-41
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• 2020

Recently, due to the development of MEMS technology, researches for the production of effective micro structures and shapes have been actively conducted. However, the process technology based on chemical etching has a number of problems such as environmental pollution and time problems due to multi-process. Various processes to cope with this process are being studied, and one of the mechanical etching processes is the powder blasting process. This process is a method of spraying fine particles, which has the advantage of being an effective process in manufacturing hard brittle materials. However, it is also a process that adversely affects the material surface roughness and material properties due to the impact of the injection of fine particles. In this study, after fabricating micro-channels in fused silica glass with excellent optical properties among the hard brittle materials, we used the nano indentation system to analyze the micro parts using nano-particles as well as machinability and surface roughness analysis of the processed surface. The analysis was performed for the effective processing of powder blasting.

• Korean Journal of Veterinary Research
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• v.44 no.1
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• pp.1-5
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• 2004

The fused and deformed lumbar vertebrae (L) of Thoroughbred horses were observed macroscopically. Results revealed five L in two cases, and six L in three cases. In two cases, the last two lumbar vertebrae were fused with each other. One case of the fused lumbar vertebra was observed in a horse with five L and the other in that with six L. In one case, the facet of second last lumbar vertebra was articulated with its anterior lumbar vertebra. On the other hand, four cases had the caudal border on the lateral tip of the lumbar transverse process partially overlapped with the cranial border of the following lumbar transverse process. In these cases, cranial and caudal tubers were formed on the overlapped part of the lumbar transverse process.

• Korean Journal of Materials Research
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• v.21 no.9
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• pp.482-485
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• 2011

$La_{0.6}Sr_{0.4}MnO_3$ (LSMO) thin films, which are known as colossal magnetoresistance materials, were prepared on fused silica thin films by conventional RF magnetron sputtering, and the interfacial reactions between them were investigated by rapid thermal processing. Various analyses, namely, X-ray diffraction, transmission electron microscopy combined with energy adispersive X-ray spectrometry, and secondary ion mass spectrometry, were performed to explain the mechanism of the interfacial reactions. In the case of an LSMO film annealed at $800^{\circ}C$, the layer distinction against the underplayed $SiO_2$ was well preserved. However, when the annealing temperature was raised to $900^{\circ}C$, interdiffusion and interreaction occurred. Most of the $SiO_2$ and part of the LSMO became amorphous silicate that incorporated La, Sr, and Mn and contained a lot of bubbles. When the annealing temperature was raised to $950^{\circ}C$, the whole stack became an amorphous silicate layer with expanded bubbles. The thermal instability of LSMO on fused silica should be an important consideration when LSMO is integrated into Si-based solid-state devices.