• Asian-Australasian Journal of Animal Sciences
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• 제30권6호
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• pp.811-818
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• 2017

Objective: This experiment was conducted to investigate the effects of fungal treated oil palm fronds (FTOPF) on performance, carcass traits, meat quality, and muscle chemical composition. Methods: Eighteen growing crossbred male goats (Thai Native${\times}$Anglo Nubian) with $18.7{\pm}2.0kg$ of initial body weight (BW) were stratified and blocked by BW in a randomized complete block design. Three diets containing 30% of oil palm fronds (OPF) either untreated (UOPF) or treated with Lentinussajor-caju (FTOPF) with or without urea (FTOPFU) were used as roughage sources in total mixed rations (TMRs). The diets were offered ad libitum and weight gain was determined. At the end of the experimental period, the harvest data and carcass characteristics of the goats were recorded, and muscular longissimus dorsi composition was determined. Results: No significant effect of fungal treated (FT) inclusion was observed in any of the feed intake, growth performance, and carcass characteristics. Likewise, no apparent effects on carcass composition and muscle chemical composition were detected in this study, except for hind leg and chump were affected (p<0.05) by FT inclusion. Conclusion: In conclusion, feeding of fungal (Lentinussajor-caju) treated oil palm frond in TMR diet did not affect performance and carcass characteristics in finishing goats.

• Natural Product Sciences
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• 제13권3호
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• pp.258-262
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• 2007

The antifungal activity of the essential oil fraction from Thymus magus, and its major component thymol, against Candida albicans was investigated in vitro and in vivo. The combined effects of the oils and clotrimazole, a commonly used antifungal drug for treatment of external candidiasis, were evaluated in this study. In experimental vaginal candidiasis the essential oil fraction of T. magnus resulted in relatively milder inhibition of fungal growth following the inoculation of test mice compared to clotrimazole. However, new fungal growth was not detected up to 12 days after cessation of treatment. In contrast, in a similar experiment using clotrimazole, C. albicans was detected in the $12^{th}$ day post-treatment with the sample. This result indicates that T. magnus oil could be a promising drug to control vaginal candidiasis. In checkerboard titer tests, the combination of clotrimazole with the essential oil fraction of T. magus or T. quinquecostatus resulted in significant synergism, with FIC indices between 0.14 and 0.27 against C. albicans, while clotrimazole combined with thymol, the major component of these oils, produced only an additive effect, with FIC indices ranging between 0.50 and 1.00. Thus, the prominent synergistic effects of clotrimazole combined with T. magus essential oil indicate that these compounds may be an effective treatment for C. albicans infections.

• 한국미생물·생명공학회지
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• 제29권4호
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• pp.253-257
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• 2001

편백의 정유성분을 추출하여 수종의 병원성세균과 진균을 대상으로 항세균 및 항진균효과를 실험하여 다음과 같은 결과를 얻었다. 편백정유는 그람 양성균인 S epidermidis, 그람 음성균인 V parahemolyticus와 P. aeruginosa에 대해서는 200 ppm부터 항세균효과를 나타냈고, P putida에 대해서는 400ppm 부터 항세균효과를 나타냈다. 항세균효과가 나타난 균주들은 편백정유의 농도가 증가할수록 효과가 높게 나타났으며 통계적으로 유의성이 관찰되었다(p<0.05) 또한 편백정유는 효모형 곰팡이인 C, albicans에 대하여 200 ppm부터 항진균효과를 나타냈으며, 사상형 곰팡이인 A, mali와 A, nidulans에 대해서는 400 ppm부터 F. oxyporum 에 대해서 100 ppm 부터 항진균효과를 보였고 편백정유의 농도가 증가할수록 균주의 성장억제대가 증가되었다. 따라서 편백을 항균제나 방부제로 사용할수 있는 가능성을 확인하였다.

• 한국토양비료학회지
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• 제43권4호
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• pp.490-497
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• 2010

근권토양으로부터 고추역병균을 포함한 다양한 식물 병원성 곰팡이에 대하여 항균활성이 강한 세균을 분리하였다. 이 세균은 16S rRNA gene서열 분석 결과 Lysobacter enzymogens로 동정되었고 LE429로 명명 하였다. LE429는 chitinase, ${\beta}-1$, 3-glucanase, protease, gelatinase, lipase 및 항생물질과 같은 다양한 이차대사산물을 분비하였다. 항생물질은 diaon HP-20 및 sephadex LH-20 컬럼크로마토그래피 및 HPLC로 정제하여, GC-EI 및 GC-CI분석을 통하여 phenylacetic acid로 동정되었다. Field 실험에서 LE429의 고추 병해 억제 효과를 조사하기 위해 LE429배양액(CB), Neem oil 용액 (NO), LE429배양액과 Neem oil 용액을 섞은 혼합액(CB+NO), 그리고 대조구로서 물(CON)을 각각 고추에 처리하였다. 고추의 수량구성요소는 일반적으로 CB 처리구가 가장 높았고, CB+NO, CON 그리고 NO 순서로 나타났다. CB 처리구에서 병원성 곰팡이는 강하게 억제 되었지만, 몇몇 해충이 발견되었다. NO 처리구에서는 해충은 발견 되지 않았지만, 병원성 곰팡이가 발견 되었다. 하지만, CB+NO 처리구에서 병원성 곰팡이 및 해충이 전혀 발견 되지 않았다. 결론적으로, 2차 대 사산물을 생산하는 LE429와 Neem oil의 혼합액은 고추에 발생하는 병원성 곰팡이와 해충에 대한 좋은 생물학적 방제제가 될 수 있다고 사료된다.

• The Plant Pathology Journal
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• 제33권6호
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• pp.582-588
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• 2017

The objective of this study was to determine inhibitory activities of four volatile plant essential oils (cinnamon oil, fennel oil, origanum oil and thyme oil) on in vitro growth of Fusarium oxysporum f. sp. fragariae causing Fusarium wilt of strawberry plants. Results showed that these essential oils inhibited in vitro conidial germination and mycelial growth of F. oxysporum f. sp. fragariae in a dose-dependent manner. Cinnamon oil was found to be most effective one in suppressing conidial germination while fennel oil, origanum oil and thyme oil showed moderate inhibition of conidial germination at similar levels. Cinnamon oil, origanum oil and thyme oil showed moderate antifungal activities against mycelial growth at similar levels while fennel oil had relatively lower antifungal activity against mycelial growth. Antifungal effects of these four plant essential oils in different combinations on in vitro fungal growth were also evaluated. These essential oils demonstrated synergistic antifungal activities against conidial germination and mycelial growth of F. oxysporum f. sp. fragariae in vitro. Simultaneous application of origanum oil and thyme oil enhanced their antimicrobial activities against conidial germination and fungal mycelial growth. These results underpin that volatile plant essential oils could be used in eco-friendly integrated disease management of Fusarium wilt in strawberry fields.

• KSBB Journal
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• 제8권3호
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• pp.295-299
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• 1993

Shake flask를 사용하여 M. piperita 세포의 현탁 배양에서의 fungal elicitor, pluronic F-B8, methylcellulose의 영향에 대하여 연구하였다. 그 결과 Rhodotorula rubra라는 균주에서 추출한 fungalelicitor를 처리하여 약 2배 정도 박하정유 생산의 증가를 관찰하였고 100 rpm의 교반속도에서 낮은 농도의 Pluronic F-68, methylcellulose 첨가에 의해 박하세포의 성장이 증진되었다.

• 한국식품영양과학회지
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• 제19권6호
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• pp.636-640
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• 1990

For a studyon the production of ${\gamma}$-linolenic acid(GLA) by fungi 3 fungal strains were isolated from soil. Their cell growth lipid content and fatty acid composition were compared in shake flask culture. Among these fungi the fungus designated as FA-007 has high lipid content(21.1%) and GLA content(15.6% of total fatty acids) The fungal strain FA-007 was tentatively identified as Mucor sp. on the basis of morphological characteristics, Fungal oil produced by this fungus was composed of 75.2% neutral lipid 5.3% glycolipid and 19.5% phospholipid. Although the GLA content in phospholipid was higher than it in neutral lipid the GLA content in neurtal lipid was high as 15.5%.

• The Plant Pathology Journal
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• 제40권2호
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• pp.192-204
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• 2024

In this study, the efficacy of the essential oil of Mentha longifolia, Achillea arabica and Artemisia absinthium plants were evaluated against important soil-borne fungal pathogens as Verticillium dahliae, Rhizoctonia solani, and Fusarium oxysporum. Essential oils were obtained from plants by hydrodistillation method and the chemical components of essential oils were determined by analyzing by gas chromatography-mass spectrometry. The main components found as piperitone oxide (13.61%), piperitenone oxide (15.55%), pulegone (12.47%), 1-menthone (5.75%), and camphor (5.75%) in M. longifolia, á-selinene 13.38%, camphor 13.34%, L-4-terpineneol 8.40%, (-)-á-Elemene 7.01%, 1,8-cineole 4.71%, and (-)-spathulenol 3.84% in A. arabica, and á-thujone (34.64%), 1,8-cineole (19.54%), pulegone (7.86%), camphene (5.31%), sabinene (4.86%), and germacrene-d (3.67%) in A. absinthium. The antifungal activities of the oils were investigated 0.05, 0.1, 0.25, 0.5, 1.00, and 2.00 μl/ml concentrations with the contact effect method. M. longifolia oil (1.00 and 2.00 μl/ml) has displayed remarkable antifungal effect and provided 100% inhibition on mycelial growth of V. dahliae, R. solani and F. oxysporum. The results obtained from this study may contribute to the development of new alternative and safe methods against soil-borne fungal pathogens.

• 한국식품영양과학회지
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• 제21권5호
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• pp.573-579
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• 1992

숙시닐화가 균체 단백질(Aspergillus fumigatus)의 여러가지 기능적 특성에 미치는 영향을 검토하였다. 균체 단백질은 succinic anhydride 2.5와 10% 첨가에 20.7 과 85.3%가 숙시닐화되었다. 숙시닐화된 균체 단백질은 흡광도, 핵산 및 탄수화물의 양은 감소하였으나 단백질소, 단백질 추출율은 증가되었다. 숙시닐화된 균체 단백질의 질소용해도는 milk casein과 soy flour 보다 32와 51% 증가되었다. 유화활성도와 유화 안정성은 균체 단백질의 숙시닐화 비율에 따라서 증가 되었으며, 80% 숙시닐화된 균체 단백질은 약 8.4배 증가율을 나타내어 숙시닐화를 증가시킴에 따라 유화활성도와 유화안정성이 증가 되었다. 숙시닐화 시킨 균체 단백질은 숙시닐화 시키지 않는 균체 단백질, milk casein 및 soy flour에 비하여 향상된 기능적 특성을 나타내었다.

• Journal of the Korean Wood Science and Technology
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• 제32권2호
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• pp.50-57
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• 2004

본 실험은 건조되지 않은 목재를 고온의 아마인유에 침지처리함으로써 목재건조와 동시에 발수효과를 얻고자 실시하였다.함수율 90% 이상의 소나무생재를 온도 150℃의 아마인유에서 6시간 침지처리하였을 때 목재함수율은 10%까지 떨어졌으며, 목재 축방향의 깊이에 관계없이 목재단면의 20%에 해당하는 아마인유가 침투하였다. 이때 목재의 강도적 손실은 발생하지 않는 것으로 나타났다. 목재표면의 천공처리는 목재의 내부할열방지에 효과적이었고, 아마인유의 깊은 침투를 유도할 수 있었다. 흡수성 시험 결과 처리재는 무처리재에 비하여 높은 발수효과를 나타냈다. 변색균 및 부후균에 대한 항균효력시험은 처리재 자체로서 균에 대한 독성 기작이 없었으나, 백색부후균 및 갈색부후균에 대해서는 무처리재보다 낮은 중량감소율을 나타내는 등 방부효력의 상승이 있었다.