• 생명과학회지
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• 제17권9호통권89호
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• pp.1294-1297
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• 2007

국내 시판 무 (Baekwoon) 의 씨앗으로부터 항 진균 단백질들을 (RAP-l,2)분리 하였으며[12] 이들 항 진균 단백질을 MALDI-TOF실험결과, 2S storage albumin, Rs-AFP등 지하부 식물의 defensin protein과[15] 일치함을 확인하였고 이에 시판되는 7종의 각각의 무 씨앗으로부터 조 단백질과 Total RNA를 분리 하여 항 효모 (Saccharomyces cerevisiae, Candida albicans.) 및 항 곰팡이 (Botrytis cenma)에 대한 항 진균성을 실험한 결과 항 곰팡이 활성은 모든 품종에서 보였으나 항 효모 활성은2 종 (Myungsan, Baekwoon) 의 무에서만 보였 다 . 또한 기존에 알려진 항 진균 단백질 (Rs-AFP)의 유전자를 Gene Bank/EMBL 에서 획득하여 씨앗으로부터 분리한 Total RNA 에 RT-PCR 한결과, 7종 중 2종은 0.2kb 의 산물이 보이지 않았다. 이들 Ks-AFP 유전자산물을 염기서열을 분석하였으며 이 염기서열에서 얻어진 아미노산 서열을 Clustal W를 이용한 pairwise alignment 분석에 의해 국내 시판 무 의 품종간 각clone의 계통수를 분석한 결과를 보고한다.

• 한국균학회지
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• 제42권1호
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• pp.79-85
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• 2014

태안반도에 자생하는 해안식물인 해국(Aster sphathulifolius Maxim.), 갯개미취(Aster tripolium L.), 갈대(Phragmites australis), 갯꾸러미풀(Puccinellia nipponica Ohwi) 의 뿌리로부터 총 42주의 내생진균이 분리되었다. 그리고 분리된 내생진균의 ITS지역의 염기서열을 이용하여 동정한 결과 모두 자낭균문에 속하며, 8종류의 목(Botryosphaeriales, Capnodiales, Diaporthales, Dothideales, Eurotiales, Helotiales, Hypocreales, and Pleosporales)과 13종류의 속(Alternaria, Aureobasidium, Cadophora, Cladosporium, Davidiella, Diaporthe, Fusarium, Gibberella, Macrophomina, Metarhizium, Neosartorya, Penicillium, and Phoma)으로 분류되었으며, Eurotiales목의 Penicillium속의 분포비율이 가장 높게 존재하였다. Shannon 다양성지수를 비교한 결과 4종의 해안식물 중 갯개미취에서 분리한 내생진균의 다양성이 가장 높게 확인되었다.

• 한국균학회지
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• 제41권2호
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• pp.67-73
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• 2013

가야시대(경남 의령, 아라가야) 돌덧널무덤 5호와 6호의 부장품 속에서 채취한 12개의 토양시료에서 곰팡이균 9종과 세균 70종을 순수분리하였다. 분리된 미생물의 16S 및 18S rDNA 염기서열을 분석한 결과, 5호 고분에서 곰팡이 5 균주, 세균 10 속, 22 균주가 발견되었으며, 6호 고분에서는 곰팡이 1 균주, 세균 6 속, 28 균주가 발견되었다. 5호 고분의 높은 미생물다양성으로 미루어 5호 고분이 6호 고분보다는 좀 더 미생물 성장에 좋은 기후조건(여름이나 초가을)에 조성되었을 것으로 추정되었다. 또한 5호 고분에 부장된 대도의 표면에서 동물의 피부에 서식하는 Staphylococcus warneri와 갯벌에서 서식하는 해양성세균 인 Bacillus aquimaris와 같은 독특한 세균이 발견됨에 따라, 매장당시의 의식에 육류와 해산물이 사용되었음을 추정할 수 있다. 결론적으로 본 연구는 미생물학적 연구가 역사학 연구의 도구가 될 수 있음을 보여주었다.

• Mycobiology
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• 제42권2호
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• pp.147-151
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• 2014

We investigated the diversity of the foliar endophytes of Korean ginseng. Endophytic fungi were isolated from healthy leaves of mountain-cultivated ginseng (MCG) and field-cultivated ginseng (FCG) at 4 sites in Chungbuk Province. A total of 24 species of fungal endophytes were identified using molecular approaches. Additionally, the diversity of these endophytic fungi was compared between MCG and FCG. The major isolated endophytes were Edenia gomezpompae and Gibberella moniliformis in the MCG and FCG samples, respectively. The results suggest that ginseng endophytes have different community structures in different environments, and this understanding may prove useful in ginseng cultivation.

• Journal of Ecology and Environment
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• 제32권1호
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• pp.13-18
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• 2009

This study was conducted to investigate ectomycorrhizal (ECM) diversity on Pinus densiflora seedlings in a disturbed pine forest. Pine seedlings less than one year old were collected from disturbed and undisturbed sites in the Mt. Songni region. The belowground ECM fungal communities colonizing P. densiflora seedlings were studied using morphotyping and DNA sequencing. The relative abundance of ECM root tips was significantly higher in the undisturbed sites than in the disturbed sites, and the ECM species diversity was lower in the disturbed sites than in the undisturbed sites. In addition, the ECM community composition was significantly different in the disturbed and undisturbed forest sites.

• Mycobiology
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• 제36권1호
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• pp.19-23
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• 2008

Red pepper (Capsicum annum L.) roots and soils representing different agricultural management practices such as conventional (CON), no-chemical (NOC), and organic farming systems (ORG) were collected from 32 farm field sites in Kyunggi, Korea to investigate the effects of these agricultural practices on arbuscular mycorrhizal (AM) symbiosis. ORG inoculum significantly increased plant growth compared to inoculum from CON and NOC. A community analysis of AM fungi (AMF) using morphological features of spores revealed that AMF spore abundance and species diversity were significantly higher in ORG than in CON. Additionally, a community analysis of AMF colonizing roots using a molecular technique revealed higher AMF diversity in ORG than in CON. These results suggest that agricultural practices significantly influence AM fungal community structure and mycorrhizal inoculum potential.

• Mycobiology
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• 제43권4호
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• pp.444-449
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• 2015

Arbuscular mycorrhizal fungi (AMF) are ubiquitous in the rhizosphere and form symbiotic relationships with most terrestrial plant roots. In this study, four strains of Rhizophagus clarus were cultured and variations in their growth characteristics owing to functional diversity and resultant effects on host plant were investigated. Growth characteristics of the studied R. clarus strains varied significantly, suggesting that AMF retain high genetic variability at the intraspecies level despite asexual lineage. Furthermore, host plant growth response to the R. clarus strains showed that genetic variability in AMF could cause significant differences in the growth of the host plant, which prefers particular genetic types of fungal strains. These results suggest that the intraspecific genetic diversity of AMF could be result of similar selective pressure and may be expressed at a functional level.

• Journal of Microbiology
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• 제45권4호
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• pp.367-370
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• 2007

Microbial diversity in decaying maize stalk was characterized by constructing and analyzing rRNA gene clone library. Total 47 OTUs were obtained from 82 bacterial clones, including Proteobacteria (64.6%), Actinobacteria (30.5%), Bacteroidetes (2.4%) and Firmicutes (2.4%). Most proteobacterial clones were members of Rhizobium, Pseudomonas and Stenotrophomonas. Eighty-four percent of Actinobacteria was related to Microbacterium. Only 14 OTUs were identified from 124 fungal clones, including Ascomycota (88%) and Basidiomycota (12%). Sixty percent of Ascomycota were members of Eupenicillium and Paecilomyces but all Basidiomycota were close to Kurtzmanomyces nectairei.

• Mycobiology
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• 제51권6호
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• pp.436-444
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• 2023

Aspergillus species play a crucial role in terrestrial environments as degraders and are well known for producing various secondary metabolites. Recently, Aspergillus species have been discovered in marine environments, exhibiting adaptability to high salinity and producing diverse secondary metabolites with valuable properties. However, limited research has focused on their marine diversity, leading to inaccurate species identification. The current study addresses this gap by investigating diverse marine habitats in the Republic of Korea, including sediment, seawater, seaweed, and marine animals. From three coasts of the Korean Peninsula, 472 Aspergillus strains were isolated from the various marine habitats. A total of 41 species were accurately identified using multigenetic markers: internal transcribed spacer, calmodulin, and b-tubulin. The findings underscore the importance of accurate identification and provide a basis for elucidating the functional role of marine-derived Aspergillus species in marine ecosystems.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.19-19
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• 2014

This study included the analysis of mushroom data collected from Mt. Chiak in Gangwon-do using various methods. Former studies of Korean mushrooms are limited by regional characters and there is less species diversity among the regions. This study tried to find a way for the forecast of mushroom distribution and appearance by indexes of species diversity. The indexes used in this study include the number of fungi (N), the number of species (S), similarity index (C), richness index (R1, R2), variety index (V1, V2), evenness index (E1, E2, E3, E4, E5), and dominance index (D1) to analyze variety of species diversity. Analyses of data of fungi using a multistage cluster sampling indicate that the average value of C for years was higher than the average value of C for areas. The mushrooms consisted of 208 species in 686 individuals in limited fungal collection from 2002 to 2003. One hundred thirty nine species in 393 individuals were collected in 2002, and 122 species 293 individuals were collected in 2003. The individuals collected in 2003 were smaller than 2002's individuals. Similarity, richness, and variety indexes' values of 2003 were reduced than 2002's values but dominance index of 2003 was increased than 2002's value. Generally the species diversity of the environment to evaluate the index of similarity, richness, and variety was a higher index; dominance index was lower than that of the surrounding environment, suggesting a good diversity. As a result, the occurrence of mushrooms in the surrounding environment and the various factors seem fell in 2002 compared to 2003. The majority genus of the limited fungal collection was Mycena genus in 63 individuals; the majority species was Laccaria laccata in 34 individuals. Ninety three species in 106 individuals were collected by the extended collection and the majority genus of the extended collection was Amanita genus in 17 individuals; the majority species was Amanita citrina (Schaeff.) Pers. which was found in 5 individuals. This demonstrates that periodical similarity's value was 0.159 is higher than special similarity's 0.119. This indicates that the probability of the appearance of same mushrooms in the same area in following year is higher than the probability of the appearance of same mushrooms in the surrounding area in same year. The value of coefficient of variation (CV), in which the amount of change is much or less by N is higher than the CV value by S. CV value of dominance index(D) was the highest r point among other indexes, and evenness index (E) was the lowest point among other indexes. The correlation matrix with 66 combinations between the indexes, the combinations with correlations was 46 combinations. These results revealed that indexes of R1, V2, and E1 were proper to represent species diversity of fungi based on the correlation matrix and the theory of statistical independence which means there is no or less mutual association. This research would contribute to the study about variable living creature by measuring method and in the future this would be used to figure out regulation about fungi with their correlation, values in ecosystem, develop improving new models about agricultural fungi species and numbers by investigating agricultural variable species.