• Journal of Korean Society of Forest Science
• /
• v.76 no.4
• /
• pp.376-389
• /
• 1987

Higher fungi (Basidiomycetes) were collected from forest stands of Kwangnung Experimental Forest, Kwangnung, Kyonggido, during the summer and fall seasons of 1976, 1984, 1985 and 1986. A total of 257 species and varieties in 104 genera were identified. Saprophytic fungi accounted for 135 species in 75 genera while mycorrhizal fungi accounted for 120 species in 28 genera. Two parasitic species were collected and they were root parasites. Among the mycorrhizal species Amanita, Russula, Lactarius, and species in the Boletaceae were the dominant taxa. The mycorrhizal fungi appear to be an important component in the essential elements needed for forest management in Korea. A reevaluation of the higher fungi previously described in Korea was made and we report 77 higher fungi previously undescribed from Korea. This is from a single experimental forest and suggests that there are many unreported higher fungi which play an important role in Korean forests.

• Journal of agriculture & life science
• /
• v.46 no.2
• /
• pp.27-33
• /
• 2012

In this study, we evaluated environmental-friendly disease control methods using essential oils, antagonistic bacteria, or cooking oil and egg yolk mixture (COY). Among the five tested plant essential oils, Eucalyptus approximans oil shown the highest effect to control of the downy mildew disease (60% disease control value). Interestingly enough, well known antagonistic bacteria against various fungal pathogens were appeared lower control efficiency than plant essential oils. In field conditions, the cooking oil and yolk mixture (COY) treatments shown significantly decreased the downy mildew incidence with 0.3% (disease conttrol value 84%) and 0.5% concentration (disease control value 91%). Our findings suggest that the COY has a great potential as the eco-friendly downy mildew disease control formulation.

• The Korean Journal of Mycology
• /
• v.47 no.3
• /
• pp.259-269
• /
• 2019

Brown spot and sheath rot of rice are caused by fungal pathogens such as Curvularia lunata, Cochliobolus miyabeanus, and Sarocladium oryzae, and cause losses such as reduced rice yield and quality, which is an enormous problem with serious long-term effects. To search biological control agents of phytopathogenic fungi, five kinds of useful Bacillus-like isolates which are excellent in extracellular enzyme activity and produce siderophore were selected from paddy soil of Sunchang in Korea. The selected isolates were tested for excellent antifungal activity against three of the phytopathogenic fungi that frequently occur in rice, and JSRB 177 strain had the most excellent antifungal activity. Based on the experimental results, JSRB 177 is finally selected as a candidate for biological control and identified to Bacillus subtilis through 16S rRNA sequence analysis. In addition, physiological characteristics of JSRB 177 confirmed by analysis of carbohydrate fermentation patterns and enzyme production ability. Based on the above results, JSRB 177 is expected to be used as a biological control agent for the rice pathogenic fungi. In the future, further studies related to industrialization such as port test and establishment of mass production process are needed.

• Asian Journal of Turfgrass Science
• /
• v.21 no.2
• /
• pp.147-154
• /
• 2007

In March of 2004, gray snow mold (Typhula blight) caused by Typhula spp. occurred on perennial ryegrass (Lolium perenne L.) and Kentucky bluegrass (Poo pratensis L.) at MuJu golf courses in Jeonbuk Province. Leaves in the affected areas were matted together and frequently covered with white to grayish mycelia. Sclerotia were formed on the leaf blade, leaf sheath, or crown regions. The fungus isolated from the diseased leaf formed whitish mycelium, clamp connections, and light pink to brown, irregular-shaped small sclerotia of less than 1.4 mm in diameter, which are characteristic to Typhula incarnata. Optimum temperature ranges for mycelial growth were $5^{\circ}C$ to $15^{\circ}C$. The causal organism was confirmed to be T. incarnata as the partial sequence of its ribosomal RNA ITS1 (internal transcribed spacer) region was 91% homologous to those of T. incarnata in GenBank database. Out of the 14 fungicides tested fur antifungal activity in vitro, 10 fungicides including iprodione, tebuconazole, polyoxin D, flutolanil, hexaconazole, tolclofos-methyl, fosetyl-Al, mepronil, pencycuron+tebuconazole, and fenarimol completely inhibited fungal growth at their recommended concentrations. In the field test, these fungicides and others such as thifluzamide and thiram effectively controlled the gray snow mold of turfgrass with some variable degrees of control efficacies.

• Journal of Sericultural and Entomological Science
• /
• v.45 no.2
• /
• pp.112-115
• /
• 2003

NIAST has developed the artificial cultivation technique of entomopathogenic fungus, Isaria sinclairii by using the silkworm. NIAST is planning to provide the silkworm farmers the guidance of the technique. Screening of pharmacologically active principles of I. sinclairii is needed to register the fungal breeding, attain the approval of food materials, and ultimately position the fungus as a high value-added product. The result of investigating the characteristics of Isaria sinclairii was showed that conidia were irregularly long oval-shaped and measured 6.33$\times$3.33 $\mu\textrm{m}$ in average The optimal condition of cultivating hyphae was pH 5.0 in acidity and 21- $25^{\circ}C$ in temperature. And the best condition of producing conidia was pH 6.0 in acidity and 16-$20^{\circ}C$ in temperature.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.5
• /
• pp.841-846
• /
• 2011

This study was conducted to obtain the information about injury caused by continuous cropping of peony (Paeonia lactiflora). Soil physico-chemical properties, characteristics of microbial distribution and diversities in the continuous cropped field with peony were analyzed. As the results, pH and organic matter content were higher in the continuous cropping soil than those in the first cropping soil. Bulk density was decreased but porosity was increased in the continuous cropping soil. As the cultivation period was lengthened in years, the populations of bacteria and actinomyces were gradually decreased, whereas fungal population was increased. It was shown that the metabolic diversity patterns of the microbial communities in the continuous cropping soil differed from that of the first cropping soil. These results indicate that deterioration of soil quality such as physico-chemical properties including a soil depth, bulk density, porosity and soil pH is related with a continuous cultivation periods, and also affect a microbial population, especially fungi.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.3
• /
• pp.479-484
• /
• 2011

Fatty acid methyl ester (FAME) profiles were used to describe differences in soil microbial communities influenced by conventional farming system (CFS), conventional farming system without pesticides (CFSWP), and organic farming system (OFS) for strawberry cultivation in controlled horticultural land. In comparison to the CFS soils, the average soil microbial biomasses of in the OFS soils were approximately 1.2 times for total FAMEs ($195nmol\;g^{-1}$), 1.4 times for total bacteria ($58nmol\;g^{-1}$), 1.5 times for Gram-negative bacteria ($27.3nmol\;g^{-1}$), 1.2 times for Gram-positive bacteria ($26.1nmol\;g^{-1}$), and 1.5 times for actinomycetes ($2.8nmol\;g^{-1}$). The microbial communities of total bacteria (p<0.05) and Gram-negative bacteria (p<0.05) in the OFS and CFSWP soils were significantly higher larger than those in the CFS soils. However, fungal structure was significantly greater in CFS than in OFS and CFSWP (p<0.05). In principal component analyses of soil microbial communities, our findings suggest that actinomycetes should be considered as potential factor responsible for the clear microbial community differentiation observed between OFS and CFS in controlled horticultural land.

• Clinical and Experimental Pediatrics
• /
• v.64 no.10
• /
• pp.504-510
• /
• 2021

Population screening of newborns is an extremely important and informative diagnostic approach that allows early identification of babies who are predisposed to the development of a number of serious diseases. Some of these diseases are known and have effective treatment methods. Neonatal screening enables the early diagnosis and subsequent timely initiation of therapy. This helps to prevent serious complications and reduce the percentage of disability and deaths among newborns and young children. Primary immunodeficiency diseases and primary immunodeficiency syndrome (PIDS) are a heterogeneous group of diseases and conditions based on impaired immune system function associated with developmental defects and characterized by various combinations of recurrent infections, development of autoimmune and lymphoproliferative syndromes (genetic defects in apoptosis, gene mutation Fas receptor or ligand), granulomatous process, and malignant neoplasms. Most of these diseases manifest in infancy and lead to serious illness, disability, and high mortality rates. Until recently, it was impossible to identify children with PIDS before the onset of the first clinical symptoms, which are usually accompanied by complications in the form of severe coinfections of a viral-bacterial-fungal etiology. Modern advances in medical laboratory technology have allowed the identification of children with severe PIDS, manifested by T- and/or B-cell lymphopenia and other disorders of the immune system. This review discusses the main existing strategies and directions used in PIDS screening programs for newborns, including approaches to screening based on excision of T-cell receptors and kappa-recombination excision circles, as well as the potential role and place of next-generation sequencing technology to increase the diagnostic accuracy of these diseases.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.34 no.3
• /
• pp.92-101
• /
• 2021

Objectives : Otomycosis is superficial fungal infection of external auditory canal accompanied with itching, pain, discharge, hearing loss, tinnitus and ear fullness. And chronic inflammation like chronic suppurative otitis media exacerbates otomycosis. The purpose of this study is to report one case of Korean medical treatment for otomycosis with chronic suppurative otitis media. Methods : This study was conducted with one case of otomycosis with chronic suppurative otitis media patient hospitalized in Ophthalmology, Otolaryngology & Dermatology Clinic of Korean medical Hospital. This patient was treated with Korean medical treatment including herbal medicine, acupuncture, pharmacopuncture and venesection. After the treatment, we took picture of external auditory canal by otoscope and evaluated subjective symptoms. Results : After the treatment, this patient had improved condition of external auditory canal and subjective symptoms. Conclusions : This study suggests that Korean medical treatment may be effective for otomycosis with chronic suppurative otitis media.

• Journal of Plant Biotechnology
• /
• v.47 no.4
• /
• pp.337-344
• /
• 2020

Plant growth-promoting microorganisms promote plant growth by supplying nutrients to roots and interacting with the intrinsic factors in plants through volatile organic compounds (VOCs). In this study, we evaluated the effect of UOS, plant growth-promoting fungi (PGPF) isolated from previous study, on the growth of Nicotiana tabacum L. var Xanthi nc. Phylogenetic analysis and GC-MS were used to identify the fungal species and the VOCs emitted by the UOS, respectively. The fresh weight of UOS-treated Nicotiana tabacum L. was 3.8 and 4.2-fold higher than that of the control groups grown in vertical and I-plates, respectively. Moreover, in the UOS-treated plants, the length of the primary root was half and the number of lateral roots were twice compared to those in control plants. The UOS was identified as Phoma sp. by studying spore and mycelial morphology and using phylogenetic analysis. GC-MS revealed that the VOC emitted by the UOS was hexamethylcyclotrisiloxane (D3). These results suggest that the UOS of Phoma sp. influences plant growth and root development through D3. We expect this UOS and its VOC, D3 to be utilized in the future to increase growth and enhance yield for other plants.