• 한국자원식물학회지
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• 제25권1호
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• pp.80-88
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• 2012

천연색소를 이용한 천연 색조화장품 개발을 위한 기초자료로 활용하고자, 15종의 천연색소를 사용하여 제조한 화장료 조성물에 대하여 항균활성 및 항산화능을 조사하였다. 실험에 사용한 6종의 균주 중 Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes, Vibrio parahaemolyticus, Aspergillus flavus에 대해 대부분의 색소혼합조성물에서 항균활성이 나타나는 것으로 확인되었으며, Proteus vulgaris에서는 다른 균주에 비해 항균활성이 상대적으로 낮았다. 색소종류별로는 자색고구마, 여주, 파프리카, 뽕잎, 지치 등의 색소 조성물에서 다른 색소 조성물에서보다 더 높은 항균활성을 보였다. 또한 DPPH 라디컬 소거능은 양파껍질, 파프리카, 적양배추, 뽕잎, 포도과피, 오디, 레드비트 색소 함유 조성물에서 상대적으로 높은 활성을 보였으며, 아질산염 소거능에 있어서는 특히 pH 1.2에서 적양배추, 포도과피, 오디, 백년초를 제외한 대부분의 색소에서 70% 이상의 높은 활성을 나타냈다. 다양한 천연색소를 이용하여 수행한 본 실험을 통해 천연색소의 높은 항균효과와 항산화능을 확인할 수 있었으며, 향후 이러한 기능성을 활용한 안전한 천연 색조화장품 개발에 유용한 자료가 될 것으로 기대한다.

• 농업과학연구
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• 제40권4호
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• pp.395-403
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• 2013

Plant factory has drawn attention in many countries in the world due to capability of environmental control not only for better yield and quality, but also for increase in functional and medicinal components of the products. In this paper, an experimental plant factory was constructed for various tests under different environmental conditions, and the operations were evaluated. A production room was constructed with adiabatic materials with dimensions of $6,900{\times}3,000{\times}2,500$ mm ($L{\times}W{\times}H$). Four sets of $2,890{\times}600{\times}2,320$ mm ($L{\times}W{\times}H$) production frame unit, each with 9 light-installed beds and an aeroponic fertigation system, resulting in 36 beds, were prepared. Accuracy and response were evaluated for each environmental control component with and without crops. Air temperature, humidity, $CO_2$ concentration, light intensity, frequency, and duty ratio, fertigation rate and scheduling were controllable from a main control computer through wireless communication devices. When the plant factory was operated without crop condition, the response times were 8 minutes for change in temperature from 20 to $15^{\circ}C$ and 20 minutes from 15 to $20^{\circ}C$; 7 minutes for change in humidity from 40 to 65%; and 4 minutes for change in $CO_2$ concentration from 450 to 1000 ppm. When operated for 24 hours with crop cultivation; average, maximum, and minimum values of temperatures were 20.06, 20.8, and $18.8^{\circ}C$; humidity were 66.72, 69.37, and 63.73%; $CO_2$ concentrations were 1017, 1168, and 911 ppm, respectively. Photosynthetic Photon Flux Density was increased as the distance from the light source decreased, but variability was greater at shorter distances. Results of the study would provide useful information for efficient application of the plant factory and to investigate the optimum environment for crop growth through various experiments.

• 한국자원식물학회지
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• 제21권1호
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• pp.12-18
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• 2008

제주도에 자생하는 양치식물 25종을 대상으로 항산화활성, ACE 저해활성, APN 저해활성 및 $\alpha$-amylase 저해활성을 검색하였다. 항산화활성은 쇠고비와 일색고사리에서 비교적 높아서 항산화 음료로 애용되고 있는 차나무 잎보다도 더 높았다. ACE 저해활성과 APN 저해활성은 전체적으로 낮았으나, 쇠고비에서 각각 26.7%와 28.2%으로 비교적 높은 저해활성을 나타내었다. $\alpha$-Amylase 저해활성은 10종이 50% 이상의 높은 활성으로 보였으며, 그 중에서 쇠고비와 홍지네고사리의 저해활성이 특히 높았다. 이상의 결과로부터 쇠고비는 항산화활성뿐만 아니라 ACE 저해활성, APN 저해활성과 $\alpha$-amylase 저해활성 모두 다른 식물에 비해 높아 생리활성 물질의 분리 및 동정 등 식품소재 및 식물생약으로 이용하기 위한 후속 연구가 기대된다.

• Environmental Engineering Research
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• 제11권5호
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• pp.266-276
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• 2006

Commonly, the flocculant is dissolved in process or recycle water in industrial plant which has many ionic materials. Therefore, the polymer degradation in aqueous solution by chemical, mechanical or bacteriological may occur, sometimes rapidly. Even if the same flocculant is dissolved, the flocculation characteristics and the properties of dissolving polymer varied with the kind of dissolving water. In this study, we try to estimate the interaction between flocculants and ionic materials in dissolving water using self inversing emulsion polymer; polyacrylamide-co-trimethyl ammonium ethyl acrylate chloride flocculants which have varying molecular weights and structures at a several conditions. The polymeric flocculant is dissolved in artificial dissolving water with Potassium Chloride (PC), Calcium Chloride anhydrous (CC), Potassium Hydroxide (PH), Sodium Chloride (SC), Sodium Bromate (SB) and Iron (II) Sulfate Heptahydrate (IS) as ionic sources. Experimental results indicate that the cationic and anionic ions in dissolving water induce the hydrolysis, degradation of cationic functional group and uncoiling of polymeric flocculants, therefore, the flocculation efficiency decreased by undesired polymer. According that result, it is important to estimate not only its structures and physical properties but also the qualities of dissolving water to optimize the efficiency.

• 한국자원식물학회지
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• 제26권3호
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• pp.374-382
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• 2013

The objective of this study was determined to evaluate ${\alpha}$-amylase, ${\alpha}$-glucosidase, pancreatic lipase inhibition in vitro and DPPH radical scavenging activity of the several Korean resources plants. The ${\alpha}$-amylase inhibitory activity of Salicornia herbacea, Erythronium japonicum (flower) and Phragmites communis (root) in water extract showed relatively high 62.8%, 66.5% and 69.3%, respectively. The ${\alpha}$-amylase inhibitory activity of Citrus junos (pericarp) and Cornus officinalis in methanol extract was found to have an effect with 32.8% in Citrus junos (pericarp) and 60.9% in Cornus officinalis. Corylopsis coreana in both water and methanol extract had the highest ${\alpha}$-glucosidase inhibitory activity of 81.7% and 89.5%, while the extract of Portulaca oleracea, Ficus carica and Citrus junos was not measured ${\alpha}$-glucosidase inhibitory activity at given experiment concentration. Depending on the extraction solvent and the plant species, it was observed that there was a significant difference in ${\alpha}$-glucosidase inhibitory activity. The pancreatic lipase inhibitory activity showed relatively higher in the methanol extract than water extract except pericarp of Citrus junos. The DPPH radical scavenging activity of selected plants was much difference between measured plant species, and showed that the increase was proportional to the concentration. These results suggested that selected plants had the potent biological activity on carbohydrate, lipid Inhibitory activity and antioxidant activity, therefore these plant resources could be a good materials to develop medicinal preparations, nutraceuticals or health functional foods for diabetes or obesity.

• 한국산학기술학회논문지
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• 제15권8호
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• pp.5176-5185
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• 2014

본 연구는 국내에서 재배되고 있는 달래의 특성을 조사하여 농가의 소득증대 및 유전자원확보를 목적으로 2010년부터 2012년 5월까지 광역단위로 달래를 수집하여 특성을 조사하였다. 달래의 특성분석은 달래가 지닌 특유의향기성분을 SPME 방법으로 분석하고, 분획물의 항산화 효과와 HaCaT, HepG2, HCT116, PC3 세포를 이용한 암세포 생장 억제 효과를 보았으며, 우리나라에 자라는 달래를 지역별로 수집하여 그 성분을 분석 및 비교하였다. 그 결과 달래에서는 총 42개의 향을 함유하고 있다고 나왔고 특히 탄화수소와 유기산의 함량이 높은 것으로 나타났으며 항산화 효과와 암세포의 성장억제 효과를 확인할 수 있었다. 이러한 달래의 높은 생리활성효과를 볼 때 향후에는 우리나라 특성화작물로써 달래를 중점적으로 육성시킬 필요성이 확인되었다.

• 한국육종학회지
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• 제41권1호
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• pp.1-8
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• 2009

This study was carried out to breed and develop high quality and functional nutrient tomato with multi disease resistance as well as a stable growing adaptation for fresh market usage under protected plastic houses cultivation. The materials were used 5 inbred lines and their 6 hybrids of large tomato group, which have been bred and developed from 1999 to 2007 in Division of Plant Resource Department of Chungnam National University. Fruit weight showed hybrid vigor effect that $F_1$ hybrids weighed more than their parent lines, fruit shape formed three type of oblate, deep oblate and globe shape, in firmness and pericarp thickness have got a high significant correlation, inbred DN611 line was measured the most firm fruit with 6.04 mm pericarp thickness. In fruit color at maturity, pink color crossed to red color appeared all red fruit color in the $F_1$ hybrids, it means red skin color is a dominant gene compared to pink skin color is a recessive gene in tomato, while between fruit skin color and shoulder part color showed no any co-relationship. The sugar content and titratable acid of $F_1$ hybrids inherited an intermediate data of their parent lines, the flavor of KP543 inbred line and the hybrid (JB535 x KP543) revealed the better taste with high brix and proper titratable acid content$^{*}$. In beta carotene content DN611 line showed 2~3 times higher than other materials so that its 3 hybrids contained an increased level of beta carotene, lycopene content was not so much difference among inbred lines and $F_1$ hybrids, of them MD508 contained higher of 8.72 mg and hybrid (JB535 x JA517) had 8.05 mg lycopene content per 100 g fruit, overall pink skin color and red skin color measured a higher lycopene content than yellow and orange skin color at ripe stage. In disease resistance test by PCR marker for Fusarium race2 (I2), Nematode (Mi1), ToMV ($Tm2^2$), Cladosporium (Cf9), (JB535 x JA517) hybrid have got multi-resistance with homozygote band in Nematode, ToMV, Cladosporium and heterozygote band in Fusarium race2. Through this breeding program we could select high quality and functional nutrient with multi resistant $F_1$ hybrids and inbred lines in tomato which are two best hybrids (JB535 x MD508), (JB535 x JA517), additionally developed high beta carotene inbred line DN611 and increased the level of lycopene inbred line MD508. These results will be very useful to make a high quality tomato variety continuously.

• Natural Product Sciences
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• 제27권2호
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• pp.115-121
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• 2021

Lentinus edodes contains functional metabolites such as polysaccharopeptides, lectins, and secondary metabolites. Fermented soybean paste is representative fermented materials in Korea, and is gradually increasing due to various biological activities. In the present study, ethanol extracts of fermented products with/without L. edodes were designated as SPL and SP, and prepared to develop safer and therapeutic functional foods with antioxidant and anti-inflammatory activities for treatment of inflammatory disorders. SP and SPL extracts exhibited antioxidant effects via inhibiting radical activities. Inflammatory mediators, nitric oxide (NO), prostaglandin E₂ (PGE₂), interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, and inducible nitric oxide synthase (iNOS) production and nuclear factor-kappa B (NF-κB) activation were down-regulated by two extracts. SPL extract more strongly enhanced the antioxidant and anti-inflammatory activities than SP extract. Its' activities shown more longer fermentation period and more strong inhibitory effects. Taken together, our results suggested that fermented product with medicinal plant has synergic effect and SPL can be a potential candidate for treatment of inflammatory bowel diseases.

• Journal of Microbiology and Biotechnology
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• 제33권10호
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• pp.1329-1336
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• 2023

Microbial fermentation is often used to improve the functionality of plant-based food materials. Herein, we investigated changes in the physicochemical and functional properties of cabbage during yeast fermentation to develop new products using fermented cabbage. Among the 8 types of food-grade yeast, both Saccharomyces cerevisiae and Saccharomyces boulardii fermented 10% cabbage powder solution (w/w) the most effectively, leaving no soluble sugars after 12 h of fermentation. In addition, the yeast fermentation of cabbage resulted in functionally positive outcomes in terms of sulforaphane content, antioxidant properties, and anti-inflammatory activity. Specifically, the yeast-fermented cabbages contained about 500% more sulforaphane. The soluble fraction (5 ㎍/ml) of yeast-fermented cabbage had no cytotoxicity in murine RAW 264.7 cells, and the radical-scavenging capacity was equivalent to 1 ㎍/ml of ascorbic acid. Moreover, cabbage fermented with S. boulardii significantly suppressed both lipopolysaccharides (LPS)-induced nitric oxide production and LPS-induced reactive oxygen species production in RAW 264.7 cells, suggesting a potential anti-inflammatory effect. These results support the idea that yeast fermentation is promising for developing functionally improved cabbage products.

• 한국식품영양과학회지
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• 제34권3호
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• pp.374-379
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• 2005

본 연구에서는 건강증진을 위해 섭취하는 다양한 식품목록을 수집하고, 최근 고시된 건강기능식품의 기준 및 규격에 관한 규정에 언급된 원료와 그렇지 않은 원료로 나누어 성별, 나이별 섭취양상을 비교함으로써 기능성에 대한 규정과 제조 관리 기준이 없는 식품 섭취 가 상대적으로 많은 집단을 찾아내고 그들이 주로 섭취하는 원료를 밝힘으로써 넓은 의미의 건강식품의 안전성 확보에 도움이 되고자하였다. 설문조사는 전국 5개 도시에서 건강증진용 식품 섭취가족이 있는 20세 이상 여성을 대상으로 하여 2004년 6월부터 8월 사이에 실시하였으며 응답자가 가계 구성원의 건강증진용 식품 섭취경험에 대해 응답하도록 하였다. 1,493명의 응답자가 대답한 가계 구성원은 환인을 포함하여 5,173명이었으며 평균나이 34.6세로 1세부터 95세까지가 대상이 되었으며 남녀 비율이 각각 48.2%, 51.8%였다. 조사된 식품 16,474개의 주원료를 원료특성에 따라 분류했을 때 영양보충용 제품이 17.60%로 가장 많았고, 과실류, 생약재 및 민간요법재, 홍삼과 인삼 제품, 기타원료, 보신식품, 채소류, 차류, 곡류 및 두류, 유산균 및 발효식품, 해조류, 버섯류, 종실류 및 견과류 순으로 분류되었다. 이들 식품원료 중 고시 원료로 분류되는 원료는 49.20%였고 그렇지 않은 원료가 50.80%였다. 건강증진을 위해 섭취한 식품 수는 1인당 평균 3.19개로 남자보다 여자가 더 많은 수의 식 품을 섭취했으며 40대가 섭취한 식품수가 가장 많았다. 고시 원료를 포함한 식품에 대해서는 1인당 평균 1.58개의 식품을 섭취해서 건강증진을 위해 섭취한 식품수의 절반 정도를 차지했다. 1인당 섭취한 건강증진용 식품수가 가장 많은 40-50대 여자들과 1인당 섭취한 식 품 중 고시 원료에 해당되지 않는 건강증진용 식품을 더 많이 섭취한 30-50대의 남자들의 건강증진용 식품의 섭취실태에 대한 지속적인 관심이 필요하며, 이들이 주로 섭취하는 생약재 및 민간요법재, 보신식품, 버섯류와 채소류, 종실류 통 과학적 행정적으로 기능성이 인정되지 않는 식품 원료에 대한 안전성 보장 방안 마련도 필요할 것으로 생각된다