• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.10a
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• pp.173-173
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• 2003

With the introduction of DNA microarrays, a high throughput analysis of gene expression is now possible as a replacement to the traditional time-consuming Southern-blot analysis. This cDNA microarray should be ahighly favored technology in the area of molecular toxicology or analysis of environmental stresses.In this study, therefore, we developed a novel cDNA microarray for analyzing stress-specific responses in japanese Medaka fish. In the design and fabrication of this stress specific functional cDNA microarray, 123 different genes in Medaka fish were selected from eighteen different stress responsive groups and spotted on a 25${\times}$75 mm glass surface. After exposure of the fish to bisphenol A which is the one of the well-known endocrine disrupting chemicals (EDCs), over 1 or 10 days, the responses of the DNA chip were found to show distinct expression patterns according to the mode of toxic actions from environmental toxicants. As a results, they showed specific gene expression pattern to bisphenol A, additionally, the chemical spesific biomarkers could be suggested based on the chip analysis data. Therefore, this chip can be used to monitor stress responses of unknown and/or known toxic chemicals using Medaka fish and may be used for the further development of biomarkers by utilizing the gene expression patterns for known contaminants.

• Molecules and Cells
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• v.20 no.2
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• pp.210-218
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• 2005

The rice genome contains at least 28 EXPA (${\alpha}$-expansin) genes. We have obtained near full-length cDNAs from the previously uncharacterized genes. Analysis of these newly identified clones together with the 12 identified earlier showed that the EXPA genes contain up to two introns and encode proteins of 240 to 291 amino acid residues. The EXPA proteins contain three conserved motifs: eight cysteine residues at the N-terminus, four tryptophan residues at the C-terminus, and a histidine-phenylalanine-aspartate motif in the central region. EXPA proteins could be divided into six groups based on their sequence similarity. Most were strongly induced in two-day-old seedlings and in the roots of one-week-old plants. However, only 14 genes were expressed in the aboveground organs, and their patterns were quite diverse. Transcript levels of EXPA7, 14, 15, 18, 21, and 29 were greater in stems, while EXPA2, 4, 5, 6, and 16 were highly expressed in both stem and sheath but not in leaf blade. EXPA1 is leaf blade-preferential, and EXP9 is leaf sheath-preferential. Most of the root-expressed genes were more strongly expressed in the dividing zone. However, the Group 2 EXPA genes were also strongly expressed in both mature and dividing zones, while EXPA9 was preferentially expressed in the elongation zone. Fourteen EXPA genes were expressed in developing panicles, with some being expressed during most developmental stages, others only as the panicles matured. These diverse expression patterns of EXPA genes suggest that in general they have distinct roles in plant growth and development.

• Journal of the Ergonomics Society of Korea
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• v.33 no.5
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• pp.377-386
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• 2014

Objective: In this study, we used resting-state fMRI data to map differences in functional connectivity between a comprehensive set of 8 distinct cortical and subcortical brain regions in healthy controls and Internet addicts. We also investigated the relationship between resting state connectivity strength and the level of psychopathology (ex. score of internet addiction scale and score of Barratt impulsiveness scale). Background: There is a lot of evidence of relationship between Internet addiction and impaired inhibitory control. Clinical evidence suggests that Internet addicts have a high level of impulsivity as measured by behavioral task of response inhibition and a self report questionnaire. Method: 15 Internet addicts and 15 demographically similar non-addicts participated in the current resting-state fMRI experiment. For the connectivity analysis, regions of interests (ROIs) were defined based on the previous studies of addictions. Functional connectivity assessment for each subject was obtained by correlating time-series across the ROIs, resulting in $8{\times}8$ matrixs for each subject. Within-group, functional connectivity patterns were observed by entering the z maps of the ROIs of each subject into second-level one sample t test. Two sample t test was also performed to examine between group differences. Results: Between group, the analysis revealed that the connectivity in between the orbito frontal cortex and inferior parietal cortex, between orbito frontal cortex and putamen, between the orbito frontal cortex and anterior cingulate cortex, between the insula and anterior cingulate cortex, and between amydgala and insula was significantly stronger in control group than in the Internet addicts, while the connectivity in between the orbito frontal cortex and insula showed stronger negative correlation in the Internet addicts relative to control group (p < 0.001, uncorrected). No significant relationship between functional connectivity strength and current degree of Internet addiction and degree of impulsitivy was seen. Conclusion: This study found that Internet addicts had declined connectivity strength in the orbitofrontal cortex (OFC) and other regions (e.g., ACC, IPC, and insula) during resting-state. It may reflect deficits in the OFC function to process information from different area in the corticostriatal reward network. Application: The results might help to develop theoretical modeling of Internet addiction for Internet addiction discrimination.

• Preventive Nutrition and Food Science
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• v.19 no.3
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• pp.220-226
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• 2014

In the present report, the effects of blanching, steaming, and high temperature/high pressure processing (HTHP) on the amino acid contents of commonly consumed Korean root vegetables, leaf vegetables, and pulses were evaluated using an Automatic Amino Acid Analyzer. The total amino acid content of the samples tested was between 3.38 g/100 g dry weight (DW) and 21.32 g/100 g DW in raw vegetables and between 29.36 g/100 g DW and 30.55 g/100 g DW in raw pulses. With HTHP, we observed significant decreases in the lysine and arginine contents of vegetables and the lysine, arginine, and cysteine contents of pulses. Moreover, the amino acid contents of blanched vegetables and steamed pulses were more similar than the amino acid contents of the HTHP vegetables and HTHP pulses. Interestingly, lysine, arginine, and cysteine were more sensitive to HTHP than the other amino acids. Partial Least Squares-Discriminate Analyses were also performed to discriminate the clusters and patterns of amino acids.

• Journal of The Korean Society of Integrative Medicine
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• v.12 no.2
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• pp.11-23
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• 2024

Purpose : The purpose of this study was to investigate the effect of exercise using a suspension device (sling and total body resistance exercise; TRX) on physical functional performance and postural balance. Methods : An experimental study comparing 2 different suspension exercise was conducted on 16 healthy college students. 16 subjects were assigned to two groups. They were classified into 8 sling group and 8 TRX group. Miniplus was used to evaluate physical functional performance. In this study, isokinetic resistance mode was used to compare and analyze seven movement patterns. Biorescue was used to evaluate postural balance. The intervention exercises in this study are as follows. Standing lean forward (SLF) using a sling and TRX was performed 3 times a week for 3 weeks. The SLF was held for 10 seconds and then rested for 15 seconds, repeated a total of 10 times for 3 sets. Results : In the TRX group, significant increases were observed in physical functional performance (p<.05). Among the differences between groups, significant differences were confirmed on the front of the right arm, the back of the left arm, and the back of the right arm. In the sling group, significant increases were observed in left, right, front, and overall dynamic balance (p<.05). A significant increase in posterior dynamic balance was confirmed in the TRX group (p<.05). There was no significant difference between groups. Conclusion : Based on the results of this study, TRX was effective in improving physical functional performance, while the sling was effective in enhancing postural balance. However, confirming the effectiveness of slings and TRX in the relationship between physical functional performance and postural balance proved inadequate. Therefore, additional research should be conducted to verify the effects of suspension.

• Journal of KIISE
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• v.42 no.3
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• pp.341-360
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• 2015

While architectural patterns provide software development solutions by providing schemas for structural organizations of software systems based on empirical knowledge, Jackson's problem frames provide a method of analyzing software problems. Problem frames are useful to understanding the software development problem, by putting emphasis on the problem domain, rather than on the solution space. Research exists that relates problem frames and software architecture, but most of this research uses problem frames only to understand given problems. Moreover, none of the existing research derives architectural patterns by considering both problem frames and quality attributes. In this paper, we propose a software architecture design method for pattern-based architecture design, by matching problem frames and architectural patterns. To that end, our approach first develops the problem model based on the problem frames approach, and then uses it to match with candidate architectural patterns, from the perspectives of both functionality, and quality attributes. Functional matching uses the problem frame diagram to match the problem model of an architectural pattern. We conduct a case study to show that our approach can systematically decide the right architectural patterns, and provide a basis for fine-grained software architecture design.

• PNF and Movement
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• v.4 no.1
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• pp.9-18
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• 2006

Purpose: The purpose of this study was to investigate the effect of Proprioceptive Neuromuscular Facilitation (PNF) applied to the unilateral upper extremity on the muscle activation of contralateral lower extremity. Twenty-two healthy subjects (mean age of 23.7 years) participated in this study. Method : PNF patterns applied on the unilateral upper extremity in all subjects were the flexion/abduction/external rotation and lifting pattern. The hold and appoximation techniques for the irradiation were applied to end range of both patterns. Muscle activations in four patterns were measured in vastus medialis, tibialis anterior, rectus femoris, and gastrocnemius medial muscles of contralateral lower extremity using surface EMG system. Each EMG value in individual muscle was normalized for maximal voluntary contraction. The data were analyzed by one factor analysis of variance with repeated measure test. Result : There were significant differences in the between-subject effect (muscles) and within-subject effect (patterns) in comparison of muscle activation by application of PNF patterns (p<.05). The irradiation led to higher activation in the flexion/abduction/external rotation pattern than that of lifting pattern in all muscles (p<.05). The approximation techniques revealed more activations than these of hold technique in all muscles (p<.05). Conclusion : These results suggest that the application of PNF patterns to the unilateral upper extremity affect on the muscle activation of contralateral lower extremity and increase according to the intensity of resistance. This mechanism of contralateral effect might provide a help to the development of treatment method for the affected side and functional improvement for the patients who have damages of central nervous system or musculoskeletal problems by orthopedic injury.

• Fashion & Textile Research Journal
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• v.20 no.1
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• pp.50-62
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• 2018

The global volume of luxury fashion industries has continuously grown with the influx of young consumers in 20s and 30s, yet little research explores luxury consumption patterns of the young consumers. Given the crucial importance of socio-psychological shopping values in luxury, the current study explores the effects of self-congruity and functional congruity on purchase intentions of luxury fashion brands, and the moderation of self-construal in the mechanisms. An online study was conducted through Google Forms with a total of 227 Korean respondents of actual luxury consumption experiences. The data was analyzed using SPSS 18.0 program. The results of the study reveal the following: (i) Among the three factors of self-congruity, ideal and social self-congruity factors have significant positive effects on purchase intentions; (ii) actual self-congruity has no statistical effect on purchase intnetions of luxury fashion brands; (iii) functional congruity has a significant positive effect on purchase intentions of luxury fashion brands; (iv) no moderation effect of self-construal was found in the relationships between the three factors of self-congruity and purchase intentions; (v) the moderation effect of independent self-construal is significant but negative in the relationship between functional congruity and purchase intentions; (vi) the effect of intdependent self-construal is not found to be significant in the relationship between functional congruity and purchase intentions. Managerial and theoretical implications are discussed.

• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.115-124
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• 2010

As the completion of genome sequencing, large collection of expression data and the great efforts in annotating plant genomes, the next challenge is to systematically assign functions to all predicted genes in the genome. Functional genome analysis of plants has entered the high-throughput stage. The generations and collections of mutants at the genome-wide level form technological platform of functional genomics. However, to identify the exact function of unknown genes it is necessary to understand each gene's role in the complex orchestration of all gene activities in the plant cell. Gene function analysis therefore necessitates the analysis of temporal and spatial gene expression patterns. The most conclusive information about changes in gene expression levels can be gained from analysis of the varying qualitative and quantitative changes of messenger RNAs, proteins and metabolites. New technologies have been developed to allow fast and highly parallel measurements of these constituents of the cell that make up gene activity. We have reviewed currently employed technologies to identify unknown functions of predicted genes including map-based cloning, insertional mutagenesis, reverse genetics, chemical mutagenesis, microarray analysis, FOX-hunting system, gene silencing mutagenesis, proteomics and chemical genomics. Recent improvements in technologies for functional genomics enable whole-genome functional analysis, and thus open new avenues for studies of the regulations and functions of unknown genes in plants.

• Environmental and Resource Economics Review
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• v.28 no.3
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• pp.437-465
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• 2019

In this study, we propose a new method to forecast long-term energy demand in Korea. Based on Chang et al. (2016), which models the time varying long-run relationship between electricity demand and GDP with a function coefficient panel model, we design several schemes to retain objectivity of the forecasting model. First, we select the bandwidth parameters for the income coefficient based on the out-of-sample forecasting performance. Second, we extend the income coefficient using the functional principal component analysis method. Third, we proposed a method to reflect the elasticity change patterns inherent in Korea. In the empirical analysis part, we forecasts the long-term energy demand in Korea using the proposed method to show that the proposed method generates more stable long term forecasts than the existing methods.