• Title/Summary/Keyword: functional module

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In vitro functional assenssment of bioartificial liver system using immobilized porcine hepatocyte spheroids

  • Lee, Ji-Hyun;Lee, Doo-Hoon;Yoon, Hee-Hoon;Jung, Doo-Hee;Park, Jung-Keug;Kim, Sung-Koo;Lee, Kwang-Woong;Lee, Suk-Koo
    • 한국생물공학회:학술대회논문집
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    • 2003.10a
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    • pp.305-306
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    • 2003
  • To treat fulminant hepatic failure (FHF) patients, various extracorporeal bioartificial liver (BAL) systems have been developed. Several requirements should be met for the development of BAL systems: hepatocytes should be cultured in a sufficiently high density; their metabolic functions should be of a sufficiently high level and duration; and the BAL systems module should permit scaling-up and aseptic handling. Several investigators have found that freshly isolated primary hepatocytes can be cultured into three dimensional, tightly packed, freely suspended, multicellular aggregates, or spheroids. These specialized cell structures exhibited enhanced liver specific functions and a prolonged differentiated state compared to cells maintained in a monolayer culture. Cells in spheroids appear to mimic the morphology and ultrastructure of the in vivo liver lobule. The ability of hepatocytes to organize into three-dimensional structures was hypothesized to contribute to their enhanced liver-specific activities. In this study, the ammonia removal rate and urea secretion rate of pig hepatocytes spheroids encapsulated in Ca-alginate bead were determined. A packed-bed bioreactor with encapsulated pig hepatocytes was devised as BAL support system. The efficacy of the system was evaluated in vitro.

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Mass Customization Oriented Modular Design of Office-chair Considering Human Body Size (다양한 신체사이즈를 고려한 사무용의자 대량맞춤생산 지향 모듈화 설계방법론)

  • Hwang, Sang-Chul;Kim, Jin-Ho;Choi, Young
    • Journal of the Korean Society for Precision Engineering
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    • v.27 no.4
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    • pp.63-71
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    • 2010
  • Modular design is a very important design methodology that allows designers to develop a product family at low product development and production cost. This design methodology is also very powerful for products that require frequent design changes due to customer requirements. Most research on modular design is focused on the modularization based on functional decomposition, physical interface and manufacturing process of parts. In this paper, we propose a modularization method that takes size of human body parts into consideration for products which have physical interactions with the products such as office chairs and sport machines. Evaluation of modular design is based on dependence measurement between every pair of components in the design. In addition we proposed a module sizing method for the maximization of customer satisfaction in MC(Mass Customization) environment.

A Study on Test Module of Smart Cards in Electronic Payment Systems (전자지불시스템에서 이용되는 스마트카드의 시험 모듈 구성에 대한 연구)

  • 김윤정;이기한
    • The Journal of Society for e-Business Studies
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    • v.9 no.1
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    • pp.303-320
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    • 2004
  • Smart card is a useful tool used in electronic payment systems and it is very important to test whether a smart card operates correctly. In this paper, we analyze previous researches on testing smart cards, such as ISO/IEC and KS standard documents, and Guideline of Card Quality Test. We also propose the functional test results done on the Highpassplus card of Korea Highway Corporation. By testing the Hipgpassplus card we can get card systems with reliable functionality and security. Furthermore, this can help developing more reliable security systems. The test results of the Highpassplus card proposed in this paper are the first research on testing smart cards in services in Korea and we expect that the test methods of smart card will be advanced based on our results.

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A Multi-Dimensional Thermal-Hydraulic System Analysis Code, MARS 1.3.1

  • Jeong, Jae-Jun;Ha, Kwi-Seok;Chung, Bub-Dong;Lee, Won-Jae
    • Nuclear Engineering and Technology
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    • v.31 no.3
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    • pp.344-363
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    • 1999
  • A multi-dimensional thermal-hydraulic system analysis code, MARS 1.3.1, has been developed in order to have the realistic analysis capability of two-phase thermal-hydraulic transients for pressurized water reactor (PWR) plants. As the backbones for the MARS code, the RELAP5/MOD3.2.1.2 and COBRA-TF codes were adopted in order to take advantages of the very general, versatile features of RELAP5 and the realistic three-dimensional hydrodynamic module of COBRA-TF. In the MARS code, all the functional modules of the two codes were unified into a single code first. Then, the source codes were converted into the standard Fortran 90, and then they were restructured using a modular data structure based on "derived type variables" and a new "dynamic memory allocation" scheme. In addition, the Windows features were implemented to improve user friendliness. This paper presents the developmental work of the MARS version 1.3.1 including the hydrodynamic model unification, the heat structure coupling, the code restructuring and modernization, and their verifications.their verifications.

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A Study on Design and Implementation of Hangul-NAVTEX Simulator (한글 NAVTEX시뮬레이터 설계 및 구현에 관한 연구)

  • 이헌택;김기문
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.3 no.4
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    • pp.819-830
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    • 1999
  • NAVTEX system is an international automated direct-printing service, broadcast on 5181kHz and 490kHz, for the promulgation of navigational and meteorological warnings and urgent information to ships. With our government's adoption of the international convention for SAR(Search and Rescue) in 1993, various trials for the installation of NAVTEX system have been executed by the government committee, relating laboratory and experts. An important consideration of the installation for NAVTEX system is the availability that could broadcast messages written in korean letter. Also, the receiver which can process the signal demodulated from the two frequencies, 518kHz and 490kHz, should be developed and supplied in domestic. In this paper, the code table and algorithm for conversions between NAVTEX characters and Korean Letters are studied, and signal processing techniques of code conversion are developed. Circuit design and implementation of the NAVTEX simulator using the Direct Digital Synthesizer are discussed, code conversion algorithm and signal processing technique of the NAVTEX transmission are programmed in its circuits. For evaluating the its functional characteristics, receiving module which has I-Q channel structure is designed. From the measurements of simulator, the characteristics show the frequency stability of the $(\pm)2Hz$ and Spurious free dynamic range is -63dBc. And the simulator can generate simultaneously wanted signal and several interfere signals. So, its capability is valuable for designers of the transmitting system and NAVTEX receiver, for provider as testing facilities of the type approval.

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Road-friendliness of Fuzzy Hybrid Control Strategy Based on Hardware-in-the-Loop Simulations

  • Yan, Tian Yi;Li, Qiang;Ren, Kun Ru;Wang, Yu Lin;Zhang, Lu Zou
    • Journal of Biosystems Engineering
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    • v.37 no.3
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    • pp.148-154
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    • 2012
  • Purpose: In order to improve road-friendliness of heavy vehicles, a fuzzy hybrid control strategy consisting of a hybrid control strategy and a fuzzy logic control module is proposed. The performance of the proposed strategy should be effectively evaluated using a hardware-in-the-loop (HIL) simulation model of a semi-active suspension system based on the fuzzy hybrid control strategy prior to real vehicle implementations. Methods: A hardware-in-the-loop (HIL) simulation system was synthesized by utilizing a self-developed electronic control unit (ECU), a PCI-1711 multi-functional data acquisition board as well as the previously developed quarter-car simulation model. Road-friendliness of a semi-active suspension system controlled by the proposed control strategy was simulated via the HIL system using Dynamic Load Coefficient (DLC) and Dynamic Load Stress Factor (DLSF) criteria. Results: Compared to a passive suspension, a semi-active suspension system based on the fuzzy hybrid control strategy reduced the DLC and DLSF values. Conclusions: The proposed control strategy of semi-active suspension systems can be employed to improve road-friendliness of road vehicles.

Incorporation of Nasutitermes takasagoensis Endoglucanase into Cell Surface-Displayed Minicellulosomes in Pichia pastoris X33

  • Ou, Jingshen;Cao, Yicheng
    • Journal of Microbiology and Biotechnology
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    • v.24 no.9
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    • pp.1178-1188
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    • 2014
  • In this study, the yeast Pichia pastoris was genetically modified to assemble minicellulosomes on its cell surface by the heterologous expression of a truncated scaffoldin CipA from Clostridium acetobutylicum. Fluorescence microscopy and western blot analysis confirmed that CipA was targeted to the yeast cell surface and that NtEGD, the Nasutitermes takasagoensis endoglucanase that was fused with dockerin, interacted with CipA on the yeast cell surface, suggesting that the cohesin and dockerin domains and cellulose-binding module of C. acetobutylicum were functional in the yeasts. The enzymatic activities of the cellulases in the minicellulosomes that were displayed on the yeast cell surfaces increased dramatically following interaction with the cohesin-dockerin domains. Additionally, the hydrolysis efficiencies of NtEGD for carboxymethyl cellulose, microcrystal cellulose, and filter paper increased up to 1.4-fold, 2.0-fold, and 3.2-fold, respectively. To the best of our knowledge, this is the first report describing the expression of C. acetobutylicum minicellulosomes in yeast and the incorporation of animal cellulases into cellulosomes. This strategy of heterologous cellulase incorporation lends novel insight into the process of cellulosome assembly. Potentially, the surface display of cellulosomes, such as that reported in this study, may be utilized in the engineering of S. cerevisiae for ethanol production from cellulose and additional future applications.

Development of a Driving Simulator for Telematics Human-Machine Interface Studies (텔레매틱스 HMI 연구를 위한 드라이빙 시뮬레이터의 개발)

  • Koo, Tae-Yun;Kim, Bae-Young;Shin, Hee-Jong;Son, Young-Tak;Suh, Myung-Won
    • Transactions of the Korean Society of Automotive Engineers
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    • v.17 no.4
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    • pp.16-23
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    • 2009
  • Driving simulators are useful tools not only to test the components of future cars but also to evaluate the telematics service and HMI (Human-Machine Interface). However driving simulators cannot be implemented to test and evaluate the telematics service system because the GPS (Global Positioning System) which contains basic functional support for the telematics module do not work in the VR (virtual reality) environment. This paper presents a method to implement telematics service to a driving simulator by developing the GPS simulator which is able to emulate GPS satellite signals consist of NMEA-0183 protocol and RS232C communication standards. It is expected that the driving simulator with the GPS simulator can be used to study HMI and human-factor evaluations of the commercial telematics system to realize the HiLES (Human-in-the-Loop Evaluation System).

The Communication Method at the Auto-Startup System using TCP/IP and VXI and Expert System(G2)

  • Kim, Jung-Soo;Joon Lyon
    • Transactions on Control, Automation and Systems Engineering
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    • v.1 no.2
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    • pp.141-146
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    • 1999
  • This paper describes the communication method of an auto-startup system. The Auto-Startup system is designed to operate a nuclear power plant automatically during the startup operation . In general , the operations during startup in existing plant have only been manually controlled by the operator. The manual operation caused to the operator mistake. The Auto-Startup system consists of the Distributed Control System(DCS) and G2 (Expert System). Also, Functional Test Facility(FTF) provides the plant's real-data for an Auto-Startup system. So, it is necessary to develop the communication method between these systems. We developed two methods ; one is a network and the other is a hardwire line. To communicate between these systems (DCS-G2 and DCS-FTF) , we developed the communication program. In case of DCS-FTF, we used the TCP/IP and VXI. BUt, in case of DCS-G2 , we , what it called , developed the bridge program using the GSI(G2 Standard Interface). We test to check the function of the important parameter, in time, for analysis of the developed communication method. The results are a good performance when we check the communication time of important parameter. We conclude that Auto-startup system could save heat-up time about at least 5 hours and reduced the change of the reactor operation and trip.

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Exploration of Molecular Mechanisms of Diffuse Large B-cell Lymphoma Development Using a Microarray

  • Zhang, Zong-Xin;Shen, Cui-Fen;Zou, Wei-Hua;Shou, Li-Hong;Zhang, Hui-Ying;Jin, Wen-Jun
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.3
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    • pp.1731-1735
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    • 2013
  • Objective: We aimed to identify key genes, pathways and function modules in the development of diffuse large B-cell lymphoma (DLBCL) with microarray data and interaction network analysis. Methods: Microarray data sets for 7 DLBCL samples and 7 normal controls was downloaded from the Gene Expression Omnibus (GEO) database and differentially expressed genes (DEGs) were identified with Student's t-test. KEGG functional enrichment analysis was performed to uncover their biological functions. Three global networks were established for immune system, signaling molecules and interactions and cancer genes. The DEGs were compared with the networks to observe their distributions and determine important key genes, pathways and modules. Results: A total of 945 DEGs were obtained, 272 up-regulated and 673 down-regulated. KEGG analysis revealed that two groups of pathways were significantly enriched: immune function and signaling molecules and interactions. Following interaction network analysis further confirmed the association of DEGs in immune system, signaling molecules and interactions and cancer genes. Conclusions: Our study could systemically characterize gene expression changes in DLBCL with microarray technology. A range of key genes, pathways and function modules were revealed. Utility in diagnosis and treatment may be expected with further focused research.