• 한국구조물진단유지관리공학회 논문집
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• 제16권3호
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• pp.92-98
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• 2012

본 연구는 터널의 유지, 보수, 보강에 필요한 가설공사용 스페이스 트러스 프레임 구조물을 소개한다. 기존 가설공사 시공방식과 장비들의 현황 및 문제점을 분석하고, 터널 설계의 핵심 구성요소를 파악함으로써, 제안된 새로운 가설 시공기술 시스템을 개발하는 과정을 순차적으로 설명한다. 그리고 개발된 가설 시스템을 발주처, 시공사, 터널 이용자 관점에서 사용성 및 경제성을 분석하고, 구조적인 안전성 검토와 최적단면 산정 평가를 상용 유한요소 해석프로그램인 ABAQUS 6.5로 구현하여 본 가설 시스템의 현장적용 기반을 구축한다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 춘계학술대회
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• pp.1047-1052
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• 2004

An efficient boundary-based technique is developed for addressing shape design sensitivity analysis in supercavitating flow problem. An analytical sensitivity formula in the form of a boundary integral is derived based on the continuum formulation for a general functional defined in potential flow problems. The formula, which is expressed in terms of the boundary solutions and shape variation vectors, can be conveniently used for gradient computation in a variety of shape design in potential flow problems. While the sensitivity can be calculated independent of the analysis means, such as the finite element method (FEM) or the boundary element method (BEM), the FEM is used for the analysis in this study because of its popularity and easy-touse features. The advantage of using a boundary-based method is that the shape variation vectors are needed only on the boundary, not over the whole domain. The boundary shape variation vectors are conveniently computed by using finite perturbations of the shape geometry instead of complex analytical differentiation of the geometry functions. The supercavitating flow problem is chosen to illustrate the efficiency of the proposed methodology. Implementation issues for and optimization procedure are addressed in this flow problem.

• 한국전산구조공학회논문집
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• 제21권1호
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• pp.59-71
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• 2008

본 논문에서는 지진하중하의 대형구조물의 시뮬레이션을 위해 실험과 해석을 병합한 분산공유 하이브리드 해석 및 실험소프트웨어 framework를 개발하였다. 제안된 소프트웨어 framework은 별도의 동적 그리고 정적 해석을 위한 프로그램의 개발이 필요 없기 때문에 일반 범용 유한요소해석 프로그램을 개발된 해석 및 실험 제어 프로그램과 interface API를 이용하여 사용할 수 있는 장점이 있다. 본 논문에서 개발된 소프트웨어 framework은 독자적 기능을 가진 module로 구성이 되어 있을 뿐만 아니라 개체지향형 프로그램 개념을 바탕으로 개발되었다. 예제를 통하여 개발된 시스템의 기능과 분산공유하이브리드 해석 및 실험에서의 유용성을 증명하였다.

• Advances in Computational Design
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• 제7권3호
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• pp.189-210
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• 2022

Deployable structures have the ability to shift from a compact state to an expanded functional configuration. By extension, reconfigurability is another function that relies on embedded computation and actuators. Linkage-based mechanisms constitute promising systems in the development of deployable and reconfigurable structures with high flexibility and controllability. The present paper investigates the deployment and reconfigurability of modular linkage structures with a pin and a sliding support, the latter connected to a linear motion actuator. An appropriate control sequence consists of stepwise reconfigurations that involve the selective releasing of one intermediate joint in each closed-loop linkage, effectively reducing it to a 1-DOF "effective crank-slider" mechanism. This approach enables low self-weight and reduced energy consumption. A kinematics and finite-element analysis of different linkage systems, in all intermediate reconfiguration steps of a sequence, have been conducted for different lengths and geometrical characteristics of the members, as well as different actuation methods, i.e., direct and cable-driven actuation. The study provides insight into the impact of various structural typological and geometrical factors on the systems' behavior.

• Structural Engineering and Mechanics
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• 제80권5호
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• pp.523-538
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• 2021

The mechanical behaviour and stability of coal mining engineering underground is significantly affected by ground water. In this study, nuclear magnetic resonance imaging (NMRI) technique was employed to determine the water distribution characteristics in coal specimens during saturation process, based on which the functional rule for water distribution was proposed. Then, using discrete element method (DEM), an innovative numerical modelling method was developed to simulate water-weakening effect on coal behaviour considering moisture content and water distribution. Three water distribution numerical models, namely surface-wetting model, core-wetting model and uniform-wetting model, were established to explore the water distribution influences. The feasibility and validity of the surface-wetting model were further demonstrated by comparing the simulation results with laboratory results. The investigation reveals that coal mechanical properties are affected by both water saturation coefficient and water distribution condition. For all water distribution models, micro-cracks always initiate and nucleate in the water-rich area and thus lead to distinct macro fracture characteristics. With the increase of water saturation coefficient, the failure of coal tends to be less violent with less cracks and ejected fragments. In addition, the core-wetting specimen is more sensitive to water than specimens with other water distribution models.

• Structural Engineering and Mechanics
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• 제85권5호
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• pp.621-633
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• 2023

The major objective of this paper is to study the receding contact problem between two functional graded layers under a flat indenter. The gravity is assumed negligible, and the shear moduli of both layers are assumed to vary exponentially along the thickness direction. In the absence of body forces, the problem is reduced to a system of Fredholm singular integral equations with the contact pressure and contact size as unknowns via Fourier integral transform, which is transformed into an algebraic one by the Gauss-Chebyshev quadratures and polynomials of both the first and second kinds. Then, an iterative speediest descending algorithm is proposed to numerically solve the system of algebraic equations. Both semi-analytical and finite element method, FEM solutions for the presented problem validate each other. To improve the accuracy of the numerical result of FEM, a graded FEM solution is performed to simulate the FGM mechanical characteristics. The results reveal the potential links between the contact stress/size and the indenter size, the thickness, as well as some other material properties of FGM.

• Molecules and Cells
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• 제46권7호
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• pp.399-413
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• 2023

cAMP responsive element-binding protein (CREB) is one of the most intensively studied phosphorylation-dependent transcription factors that provide evolutionarily conserved mechanisms of differential gene expression in vertebrates and invertebrates. Many cellular protein kinases that function downstream of distinct cell surface receptors are responsible for the activation of CREB. Upon functional dimerization of the activated CREB to cis-acting cAMP responsive elements within the promoters of target genes, it facilitates signal-dependent gene expression. From the discovery of CREB, which is ubiquitously expressed, it has been proven to be involved in a variety of cellular processes that include cell proliferation, adaptation, survival, differentiation, and physiology, through the control of target gene expression. In this review, we highlight the essential roles of CREB proteins in the nervous system, the immune system, cancer development, hepatic physiology, and cardiovascular function and further discuss a wide range of CREB-associated diseases and molecular mechanisms underlying the pathogenesis of these diseases.

• Wind and Structures
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• 제38권5호
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• pp.341-354
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• 2024

This paper presents a theoretical method to deal with the aerodynamic performance and pitch optimization of the horizontal axis wind turbine blades at low wind speeds. By considering a blade element, the functional relationship among the angle of attack, pitch angle, rotational speed of the blade, and wind speed is derived in consideration of a quasi-steady aerodynamic model, and aerodynamic loads on the blade element are then obtained. The torque and torque coefficient of the blade are derived by using integration. A polynomial approximation is applied to functions of the lift and drag coefficients for the symmetric and asymmetric airfoils respectively, where specific expressions of aerodynamic loads as functions of the angle of attack (which is a function of pitch angle) are obtained. The pitch optimization problem is investigated by considering the maximum value problem of the instantaneous torque of a blade as a function of pitch angle. Dynamic pitch laws for HAWT blades with either symmetric or asymmetric airfoils are derived. Influences of parameters including inflow ratio, rotational speed, azimuth, and wind speed on torque coefficient and optimal pith angle are discussed.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.20-20
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• 2017

Plant genomes include heterochromatic loci that consist of repetitive sequences and transposable elements. LTR retrotransposon is the major class of transposons in advanced plants in terms of proportion in plant genome. The elements contribute not only to genome size but also to genome stability and gene expression. A number of cases have been reported transposon insertions near genic regions affect crop traits such as fruit pigments, stress tolerance, and yields. Functional LTR retrotransposons produce extrachromosomal DNA from genomic RNA by reverse transcription that takes place within virus-like-particles (VLPs). DECREASED DNA METHYLATION 1 (DDM1) plays important roles in maintaining DNA methylation of heterochromatin affecting all sequence contexts, CG, CHG, and CHH. Previous studies showed that ddm1 mutant exhibits massive transcription of retrotransposons in Arabidopsis, but only few of them were able to create new insertions into the genome. RNA-dependent RNA POLYMERASE 6 (RDR6) is known to function in restricting accumulation of transposon RNA by processing the transcripts into 21-22 nt epigenetically activated small interfering RNA (easiRNA). We purified VLPs and sequence cDNA to identify functional LTR retrotransposons in Arabidopsis ddm1 and ddm1rdr6 plants. Over 20 LTR copia and gypsy families were detected in ddm1 and ddm1rdr6 sequencing libraries and most of them were not reported for mobility. In ddm1rdr6, short fragments of ATHILA gypsy elements were detected. It suggests easiRNAs might regulate reverse transcription steps. The highest enriched element among transposon loci was previously characterized EVADE element. It has been reported that active EVADE element is more efficiently silenced through female germline than male germline. By genetic analyses, we found ddm1 and rdr6 mutation affect maternal silencing of active EVADE elements. DDM1-GFP protein accumulated in megaspore mother cell but was not found in mature egg cell. The fusion protein was also found in early embryo and maternal DDM1-GFP allele was more dominantly expressed in the embryo. We observed localization of DDM1-GFP in Arabidopsis and DDM1-YFP in maize and found the proteins accumulated in dividing zone of root tips. Currently we are looking at cell cycle dependency of DDM1 expression using maize system. Among 10 AGO proteins in Arabidopsis, AGO9 is specifically expressed in egg cell and shoot meristematic cells. In addition, mutation of AGO9 and RDR6 caused failure in maternal silencing, implying 21-22 nt easiRNA pathway is important for retrotransposon silencing in female gametophyte or/and early embryo. On the other hand, canonical 24 nt sRNA-directed DNA methylation (RdDM) pathways did not contribute to maternal silencing as confirmed by this study. Heat-activated LTR retrotransposon, ONSEN, was not silenced by DDM1 but the silencing mechanisms require RdDM pathways in somatic cells. We will propose distinct mechanisms of LTR retrotransposons in germline and somatic stages.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.97-97
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• 2017

Plant genomes include heterochromatic loci that consist of repetitive sequences and transposable elements. LTR retrotransposon is the major class of transposons in advanced plants in terms of proportion in plant genome. The elements contribute not only to genome size but also to genome stability and gene expression. A number of cases have been reported transposon insertions near genic regions affect crop traits such as fruit pigments, stress tolerance, and yields. Functional LTR retrotransposons produce extrachromosomal DNA from genomic RNA by reverse transcription that takes place within virus-like-particles (VLPs). DECREASED DNA METHYLATION 1 (DDM1) plays important roles in maintaining DNA methylation of heterochromatin affecting all sequence contexts, CG, CHG, and CHH. Previous studies showed that ddm1 mutant exhibits massive transcription of retrotransposons in Arabidopsis, but only few of them were able to create new insertions into the genome. RNA-dependent RNA POLYMERASE 6 (RDR6) is known to function in restricting accumulation of transposon RNA by processing the transcripts into 21-22 nt epigenetically activated small interfering RNA (easiRNA). We purified VLPs and sequence cDNA to identify functional LTR retrotransposons in Arabidopsis ddm1 and ddm1rdr6 plants. Over 20 LTR copia and gypsy families were detected in ddm1 and ddm1rdr6 sequencing libraries and most of them were not reported for mobility. In ddm1rdr6, short fragments of ATHILA gypsy elements were detected. It suggests easiRNAs might regulate reverse transcription steps. The highest enriched element among transposon loci was previously characterized EVADE element. It has been reported that active EVADE element is more efficiently silenced through female germline than male germline. By genetic analyses, we found ddm1 and rdr6 mutation affect maternal silencing of active EVADE elements. DDM1-GFP protein accumulated in megaspore mother cell but was not found in mature egg cell. The fusion protein was also found in early embryo and maternal DDM1-GFP allele was more dominantly expressed in the embryo. We observed localization of DDM1-GFP in Arabidopsis and DDM1-YFP in maize and found the proteins accumulated in dividing zone of root tips. Currently we are looking at cell cycle dependency of DDM1 expression using maize system. Among 10 AGO proteins in Arabidopsis, AGO9 is specifically expressed in egg cell and shoot meristematic cells. In addition, mutation of AGO9 and RDR6 caused failure in maternal silencing, implying 21-22 nt easiRNA pathway is important for retrotransposon silencing in female gametophyte or/and early embryo. On the other hand, canonical 24 nt sRNA-directed DNA methylation (RdDM) pathways did not contribute to maternal silencing as confirmed by this study. Heat-activated LTR retrotransposon, ONSEN, was not silenced by DDM1 but the silencing mechanisms require RdDM pathways in somatic cells. We will propose distinct mechanisms of LTR retrotransposons in germline and somatic stages.