Consumption has generally become more fragmented, hedonic and individual specific, satisfying not only functional but also emotional needs. In parallel, customer satisfaction is now thought to be both a cognitive and affective response, and the closely related concept of job satisfaction is commonly seen as an emotional reaction. The reasoning within quality management does, however, still lean heavily toward cognitive judgements (i.e. performance ratings), the emotional component clearly being under explored. Further, performance variables have shown not to be significant in predicting satisfaction for certain 'experience products', the effect fully mediated by emotions. As a consequence a cognitive judgement based quality concept has lost its ability to predict satisfaction, which clearly contradicts with the modem quality definition, stressing quality as the ability to satisfy the customer. Emotions have however entered the quality discourse and it has been proposed that having customers that are merely feeling satisfied will not suffice. Instead, there has been a plethora of executive exhortations in the trade press calling on business to 'delight the customer'. Strategies for doing so have however usually been imprecise and unclear, and the different drivers of delight and satisfaction are not well explored. This paper aims to complement the previous cognitive dominance by exploring the multiple emotional responses involved in customer satisfaction. A conclusion being that we currently are measuring something, in terms of satisfied, that is more or less independent of what we aim for, in terms of delight. It is also most likely that - depending on the situation, product, and person - other positive and negative emotions are more important outcomes of purchase and usage than merely satisfaction. It is questioned whether a single, summary response such as satisfaction is feasible or even desirable.
Complementation cloning of the argC, E, B, D, F, and G genes in Corynebacterium glutamicum was done by transforming the genomic DNA library into the corresponding arginine auxotrophs fo Escherichia coli. Recombinant plasmids containing 6.7 kb and 4.8kb fragments complementing the E. coli argB mutant were also able to complement the E. coli argC, E, A, D, and F mutants, indicating the clustered organization of the arginine biosynthetic genes within the cloned DNA fragments. The insert DNA fragments in the recombinant plasmids, named pRB1 AND pRB2, were physically mapped with several restriction enzymes. By further subcloning the entire DNA fragment containing the functions and by complementation analysis, we located the arg genes in the order of ACEBDF on the restriction map. We also determined the DNA nucleotide sequence of the fragment and report here the sequence of the argB gene. When compared to that with the mutant strain, higher enzyme activity of N-acetylglutamate kinase was detected in the extract of the mutant carrying the plasmid containing the putative argB gene, indicating that the plasmid contains a functional argB gene. Deduced amino acid sequence of the argB gene shows 45%, 38%, and 25% identity to that from Bacillus strearothermophilus, Bacillus substilus, and E. coli respectively. Our long term goal is genetically engineering C. glutamicum which produces more arginine than a wild type strain does.
The purpose of this study was examining the effects of family sandplay therapy with structural family therapy in improving the family strengths of children with emotional and behavioral disorders. First, we looked for a way to combine family sandplay therapy with structural family therapy and formulated an approach that could maximize the strengths of each theory and complement each other's weaknesses. And then we applied family sandplay therapy together with structural family therapy and analyzed its effects on family strengths. The subjects of this study were 10 families of children with emotional and behavioral disorders, and they were divided into an experimental group with 5 families and a control group with the other 5. To the experimental group received 16 sessions of family sandplay therapy with structural family therapy. In order to prove the effectiveness of family sandplay therapy with structural family therapy on family strengths, a family relationship( husband-wife relationship, parent-child relationship, and sibling relationship) scale and a family function(family cohesion, family adaptability) scale were used. The main findings of this study are as follows. First, the family relationships of the families with emotional-behavioral disorder children were improved after the application of family sandplay therapy with structural family therapy. Second, the application of family sandplay therapy with structural family therapy improved the family function of families with emotional-behavioral disorder children. Third, in the course of family sandplay therapy with structural family therapy, family sand tray was changed in a pro-healing direction, and family structure was also transformed from a dysfunctional structure to a functional one. As previously stated, family sandplay therapy with structural family therapy for families of children with emotional and behavioral disorders enhanced family strengths through improving family relationships and restoring family functions.
The Journal of the Korea institute of electronic communication sciences
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v.13
no.1
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pp.207-212
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2018
As a data analysis tool which is becoming popular in the Big Data era, R is rapidly expanding its user range by providing powerful statistical analysis and data visualization functions. Major advantage of R is its functional scalability based on open source, but its scale scalability is limited, resulting in performance degrades in large data processing. RHadoop, one of the extension packages to complement it, can improve data analysis performance as it supports Hadoop platform-based distributed processing of programs written in R. In this paper, we evaluate the validity of RHadoop by evaluating the performance improvement of RHadoop in real medical big data analysis. Performance evaluation of the analysis of the medical history information, which is provided by National Health Insurance Service, using R and RHadoop shows that RHadoop cluster composed of 8 data nodes can improve performance up to 8 times compared with R.
Chin, Byung-Doo;Suh, Min-Chul;Kim, Mu-Hyun;Kang, Tae-Min;Yang, Nam-Choul;Song, Myung-Won;Lee, Seong-Taek;Kwon, Jang-Hyuk;Chung, Ho-Kyoon;Wolk, Martin B.;Bellmann, Erika;Baetzold, John P.
Journal of Information Display
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v.4
no.3
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pp.1-5
/
2003
Laser-Induced Thermal Imaging (LITI) is a laser addressed patterning process and has unique advantages such as high-resolution patterning with over all position accuracy of the imaged stripes of within 2.5 micrometer and scalability to large-size mother glass. This accuracy is accomplished by real-time error correction and a high-resolution stage control system that includes laser interferometers. Here the new concept of hybrid system that complement the merits of small molecule and polymer to be used as an OLED; our system can realize easy processing of light emitting polymers and high luminance efficiency of small molecules. LITI process enables the stripes to be patlerned with excellent thickness uniformity and multi-stacking of various functional layers without having to use any type of fine metal shadow mask. In this study, we report a full-color hybrid OLED using the multi-layered structure consisting of small molecules and polymers.
Journal of Dental Rehabilitation and Applied Science
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v.19
no.3
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pp.239-245
/
2003
The occurrence and pattern of tooth wear are related to cultural, dietary, occupational, and geographic factors, Excessive occlusal wear can result in pulpal exposure, occlusal disharmony, functional inability. Surface loss can be differentiated into 3 general causal categories: i) mechanical loss, which includes attrition, ii) abrasion chemical loss including erosion, and finally iii) a proposed biomechanical category described as abfraction. Considering that mechanical wear is frequently asymptomatic, patients may have parafunctional habit. Structured clinical decision analysis in fixed prosthodontics, as other branches of dentistry do, allows the practitioner to consider the patient's problems more thoroughly based on the clinical data and extenuation factors. This discipline of decision making is intended to complement the experience level and educational background of the clinician in assisting the patient through the decision process. Additionally, CDA helps the clinician define not only the pre-existing condition of the patient prior to irreversible therapy, but also better treatment strategies for the patient over an extended period. The systematic nature of decision analysis stimulates the dentists to pay further attention to those factors, which is germane to the overall complexity of the case, and exclude factors, which have little influence on its final outcome. Further implementation of computerized databases, procedural outcome probabilities based on clinical and laboratory studies and the clinical experience of those who use it, may provide quite a promising future in the field of structured and formalized clinical decision analysis.
Nguyen, Ngoc Quy;Lee, Sang-Choon;Yang, Tae-Jin;Lee, Ok Ran
Journal of Ginseng Research
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v.41
no.3
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pp.403-410
/
2017
Background: Prenyltransferases catalyze the sequential addition of isopentenyl diphosphate units to allylic prenyl diphosphate acceptors and are classified as either trans-prenyltransferases (TPTs) or cis-prenyltransferases (CPTs). The functions of CPTs have been well characterized in bacteria, yeast, and mammals compared to plants. The characterization of CPTs also has been less studied than TPTs. In the present study, molecular cloning and functional characterization of a CPT from a medicinal plant, Panax ginseng Mayer were addressed. Methods: Gene expression patterns of PgCPT1 were analyzed by quantitative reverse transcription polymerase chain reaction. In planta transformation was generated by floral dipping using Agrobacterium tumefaciens. Yeast transformation was performed by lithium acetate and heat-shock for $rer2{\Delta}$ complementation and yeast-two-hybrid assay. Results: The ginseng genome contains at least one family of three putative CPT genes. PgCPT1 is expressed in all organs, but more predominantly in the leaves. Overexpression of PgCPT1 did not show any plant growth defect, and its protein can complement yeast mutant $rer2{\Delta}$ via possible protein-protein interaction with PgCPTL2. Conclusion: Partial complementation of the yeast dolichol biosynthesis mutant $rer2{\Delta}$ suggested that PgCPT1 is involved in dolichol biosynthesis. Direct protein interaction between PgCPT1 and a human Nogo-B receptor homolog suggests that PgCPT1 requires an accessory component for proper function.
Gold compounds depress phagocytic cell responses, including chemotaxis, and respiratory burst. However, the effects of gold compounds on the function of phagocytic cells are variable according to the preparation of medicine. In this study, effect of tetrachloroauric acid on activated neutrophil responses, including respiratory burst, lysosomal enzyme release and change of intracellular $Ca^{2+}$ level and on the synthesis of interleukin-8 and granulocyte-macrophage colony stimulating factor by macrophages was studied. This study further examines how gold compounds affect the activation processes. The respiratory burst stimulated by complement C5a, degraded IgG and PMA in neutrophils was inhibited by tetrachloroauric acid. In contrast to C5a and degraded IgG, PMA-stimulated superoxide production was weakly inhibited by tetrachloroauric acid. Staurosporine, genistein, EGTA and verapamil inhibited superoxide and $H_2O_2$ production caused by C5a and degraded IgG. PMA-stimulated superoxide production was inhibited by staurosporine but was not affected by genistein. Tetrachloroauric acid, genistein, EGTA and verapamil inhibited the release of acid phosphatase and myeloperoxidase, while the effect of staurosporine was not detected. The synthesis of interleukin-8 and granulocyte-macrophage colony stimulating factor by $interleukin-1{\beta}$ in macrophages was inhibited by tetrachloroauric acid. Preincubation with tetrachloroauric acid, genistein, EGTA and verapamil, the elevation of [$Ca^{2+}_i$] evoked by C5a was inhibited. Store-regulated $Ca^{2+}$ entry in thapsigargin-pretreated neutrophils was decreased by the addition of tetrachloroauric acid and genistein. The effect of staurosporine on intracellular $Ca^{2+}$ mobilization was not observed. In conclusion, tetrachloroauric acid may suppress neutrophil responses through its inhibitory action on elevation of intracellular $Ca^{2+}$ level and protein kinase C. It might exhibit an inhibitory effect on the action of protein tyrosine kinase. Tetrachloroauric acid depresses cytokine production by macrophages.
The Journal of the Convergence on Culture Technology
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v.7
no.1
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pp.134-139
/
2021
This paper discusses the types and functions of English subordinate clauses, whether English subordinate clauses (SC) are headed by a Complementizer (CP) or headed by a lexical (but not functional) Preposition (PP). Furthermore, unlike the standard classification, the current paper provides a finer-grained analysis and classification of English SCs. The traditional or prescriptive view on the functions of English SCs includes Noun SC such as complement clauses, Adjectival SC including relative clauses, and Adverbial SCs that cover a garden variety of subordinators. Added to the existing classification of subordination in English is what I notate as Verbless subordinate clause (V-less SC). Of these 4 different types of subordinate clauses with different functions, properties, and distributions, Subjectless Verbless subordinate clause is further divided into Smallest small clause (SSC) which accounts for English subordination mechanism more uniformly and consistently with respect to their clausal architecture, especially when the subordinate clause is neither PP nor CP.
Journal of the Korean Society of Clothing and Textiles
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v.31
no.12
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pp.1672-1681
/
2007
Cationization is effective to complement the defects of Tencel blended fabrics by introducing new functions. For this purpose, we used chitosan, which is congenial to the human body, free of pollution, and easily reacted. Then, we compared it to the Tencel single fabrics. To perform such effective cationization, the fabrics were treated with chitosan after NaOH pretreatment and enzyme treatment thereof. After that, the fabrics were treated with a crosslinking agent and a softner. The dyeing property of the cationized Tencel blended fabrics and reactive dye, which is a type of anionic dye, show a high concentration in neutral salt and excellent repulsive power between the fabrics and the decreased dyes. The dyeing property of the chitosan treated fabrics represented better performances than that of untreated fabric in the lower concentration of neutral salt. Meanwhile, when it was dyed with certain acid dyes, the dyeing property of the chitosan treated fabrics showed better results due to the reaction of an amine group, which was introduced by chitosan treatment. Thus, the verification of the cationization of the Tencel blended fabrics was performed. The washing fastness of the Tencel blended fabrics showed a little bit better than that of the Tencel single fabric, and it represented a better performance in the dye with a reactive dye than that of an acid dye.
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