• Journal of Pharmaceutical Investigation
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• v.35 no.2
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• pp.75-80
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• 2005

A promising application of Lactococcus lactis is its use as live vehicles for production and delivery of heterologous proteins of vaccines and therapeutic substances. Because L. lactis has GRAS ('generally regarded as safe') status, we tested whether L. lactis could function as the carrier of the Ll protein of human papillomavirus (HPV) type 16. The RNA level expression of Ll gene was detected in L. Lactis. The Ll protein was expressed in L. lactis with Ll gene. The growth of strains L. lactis with an empty plasmid (pAMJ328) and L. lactis with Ll-encoding plasmid (pAMJ328-Ll) was slightly decreased in comparison with the growth of strains L. lactis (wild type). However, all the three strains of L. lactis maintained the ability to ferment sugars primarily into lactic acid, indicating that Ll protein did not affect the biochemical property of L. lactis. These results suggest that L. lactis, capable of carrying Ll protein, might be further developed as a biocompatible oral protein delivery system.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.62 no.3
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• pp.117-126
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• 2013

This paper is written for the development and application of boiler furnace pressure control algorithm and distributed control system of coal-fired power plant by the steps of design, coding, simulation test, site installation and site commissioning test. The control algorithms were designed in the shape of cascade control for two parts of furnace pressure control and induced draft fan pitch blade by standard function blocks. This control algorithms were coded to the control programs of distributed control systems. The simulator for coal-fired power plant was used in the test step and automatic control, sequence control and emergency stop tests were performed successfully like the tests of the actual power plant. The reliability was obtained enough to be installed at the actual power plant and all of distributed control systems had been installed at power plant and all signals were connected mutually. Tests for reliability and safety of plant operation were completed successfully and power plant is being operated commercially. It is expected that the project result will contribute to the safe operation of domestic new and retrofit power plants, the self-reliance of coal-fired power plant control technique and overseas business for power plant.

• Journal of KIISE
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• v.44 no.6
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• pp.637-642
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• 2017

Honey Encryption (HE) is a technique to overcome the weakness of a brute-force attack of the existing password-based encryption (PBE). By outputting a plausible plaintext even if the wrong key is entered, it provides message recovery security which an attacker can tolerate even if the attacker tries a brute-force attack against a small entropy secret key. However, application of a cipher that requires encryption padding to the HE present a bigger problem than the conventional PBE method. In this paper, we apply a typical block cipher (AES-128) and a stream cipher (A5 / 1) to verify the problem of padding through the analysis of the sentence frequency and we propose a safe operation method of the HE.

• Journal of Microbiology and Biotechnology
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• v.30 no.8
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• pp.1124-1131
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• 2020

Techniques used for the regulation of gene expression facilitate studies of gene function and treatment of diseases via gene therapy. Many tools have been developed for the regulation of gene expression in mammalian cells. The Lac operon system induced with isopropyl β-D-1-thiogalactopyranoside (IPTG) is one of the employed inducible systems. IPTG mimics the molecular structure of allolactose and has a strong affinity for the corresponding repressor. IPTG is known to rapidly penetrate into mammalian cells and exhibits low toxicity. In the present study, we developed a new inducible expression system that could regulate the expression of genes in mammalian cells using IPTG. Here we confirm that unlike other vector systems based on the Lac operon, this expression system allows regulation of gene expression with lactose in the mammalian cells upon transfection. The co-treatment with IPTG and lactose could improve the regulatory efficiency of the specific target gene expression. The regulation of gene expression with lactose has several benefits. Lactose is safe in humans as compared to other chemical substances and is easily available, making this technique very cost-effective.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.18 no.3
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• pp.77-83
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• 2010

"The prohibition of careless or reckless flight" is the regulation specified not only in the national air law but ICAO ANNEX and FAR. This article(item) has not been categorized properly unlike other items and the question such as why this is described as a fundamental and essential act can be answered only by the party subjected to administrative measures in case of Korea and this kind of violation is so rare that it is not easy to understand the legal meaning and the function of the term, "The prohibition of careless or reckless flight" In case of U.S where aviation cases are common, the distinction between the term "careless" or "reckless" operation depends on whether to recognize the given situation. Some incidents happened by failing to aware NOTAM, violating ATC, or T/W landing where a pilot did not recognize the violation itself are considered to be "Careless" flight. Others such as low altitude high speed flight, approximate flight, Rejecting ATC instruction where a pilot intends to or is remiss in safety are regarded as "Reckless" flight. For pilots who are required to take the highest level of care from preparing for flight to stopping engines or completely disembarking passengers from a plane, the clear understanding of the most basic concept of "careless" or "reckless" flight should be emphasized for the safe flight and it is the time for the authorities to set a standard for proper measures by definite legal interpretations.

• Korean Journal of Artificial Intelligence
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• v.4 no.1
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• pp.8-10
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• 2016

These days, citizens have a lot of ways to get access to IT. In the past, they tended to neglect IT that was thought to be difficult. But, currently, everyone can manufacture and get access not only software but also hardware when he has an idea. Arduino is used. Rinnai had recently released new product named Smart Sensor Range. Safe consumer who gave priority to the safety made new trend gave attention to fire prevention and smart sensor range. The ones who buy gas range prefer safety to economic advantage and/or fire power. The safety system does not always prevent fire accident. This study makes design and produces alarm that perceives temperature of pot when boiling. Not only temperature sensor but also alarm sensor was used to make alarm of boiling water and to give convenient living life. The arduino can be used at practical life to make products for various kinds of people. The invention can give convenience to housewives at kitchen, children and many persons making use of gas range. Another function can be added to develop. This arduino can develop a lot of products by using the study and other designs.

• Earthquakes and Structures
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• v.12 no.5
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• pp.583-589
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• 2017

In this study, result of a field investigation of railway traffic-induced vibrations is provided to examine acceptability levels of ground vibration and to evaluate the serviceability of a liquid-storage tank. Free field attenuation of the amplitudes as a function of distance is derived by six accelerometers and compared with a well-known half-space Bornitz's analytical solution which considers the loss of the amplitude of waves due to geometrical damping and material damping of Rayleigh. Bornitz's solution tends to overlap vertical free field vibration compared with in-situ measured records. The vibrations of the liquid-storage tank were compared with the USA, Federal Transportation Railroad Administration (FTA) criteria for acceptable ground-borne vibrations and with the criteria in DIN 4150-3 German standard. Comparing the thresholds stated in DIN 4150-3, absolute peak particle velocities are within the safe limits, however according to FTA velocity level at the top of the water tank exceeds the allowable limits. Furthermore, it is intended to indicate experimentally the effect of the kinematic interaction caused by the foundation of the structure on the free-field vibrations.

• The Journal of Pediatrics of Korean Medicine
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• v.31 no.3
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• pp.24-36
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• 2017

Objectives The purpose of this study is to provide clinical evidence of herbal medicine treatment for cerebral palsy by reviewing randomized controlled trials conducted in China. Methods We searched literatures dated up to 17 May, 2017 in China National Knowledge Infrastructure (CNKI), and evaluated methodological quality of those studies using 'Risk of Bias' tool. Results Sixteen studies were selected for analysis. These studies indicated that the total effective rate, motor function, self-care, muscle spasticity were significantly improved in the herbal medicine treatment group, as compared to the control group. The most-commonly-used herbs were Poria (茯?), Astragali Radix (黃?), Glycyrrhizae Radix (甘草), and Atractylodis Rhizoma Alba (白朮). There were no serious adverse events found that were associated with herbal medicine treatment. The methodological quality of included studies was generally unclear or low. Conclusions This study shows that the herbal medicine treatment can be effective and safe in treating cerebral palsy. Further well-designed clinical trials with high methodological quality and appropriate assessment tools need to be performed to solidify these findings.

• The KIPS Transactions:PartD
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• v.12D no.3 s.99
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• pp.499-506
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• 2005

A hash chain is a structure organized by hash function with high speed in computation. Systems using the hash chain are using extensively in various cryptography applications such as one-time passwords, server-supported signatures and micropayments. However, the most hash chain based on the system using pre-paid method provides anonymity but has the problem to increase payment cost. In this paper, we propose a new hash chain based on the micropayment system to keep user anonymity safe through blind signature in the withdrawal process of the root value without disclosing privacy information, and to improve efficiency by using secret key instead of public key in the system without the role of certificate.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2001.05b
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• pp.38-41
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• 2001

In this work, we have been studied the characteristics of each nitride film for the optimization of PMD(pre-metal dielectric) liner nitride process, which can applicable in the recent semiconductor manufacturing process. The deposition conditions of nitride film were splited by PO (protect overcoat) nitride, baseline, low hydrogen, high stress and low hydrogen, respectively. And also we tried to catch hold of correlation between BPSG(boro-phospho silicate glass) deposition and densification. Especially, we used FTIR area method for the analysis of density change of Si-H bonding and Si-NH-Si bonding, which decides the characteristics of nitride film. To judge whether the deposited films were safe or not, we investigated the crack generation of wafer edge after BPSG densification, and the changes of nitride film stress as a function of RF power variation.