• Title/Summary/Keyword: function level evolution

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Isolation of HRD3 gene, a homologous RAD3 gene from fission yeast Schizosaccharomyces pombe

  • Choi, In-Soon;Jin, Yong-Hwan;Park, Sang-Dai
    • Environmental Mutagens and Carcinogens
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    • v.16 no.2
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    • pp.77-82
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    • 1996
  • The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA-RNA helicase activies. To examine the extent of conservation of structure and function of RAD3 during eukaryotic evolution, we have cloned the RAD3 homolog, HRD3, from the distantly related yeast Schizosaccharomyces pombe. Here, we report the partial cloning and characterization of HRD3 gene (Homologous of RAD3 gene) which was isolated by PCR amplification using conserved domain of Saccharomyces cerevisiae RAD3 gene. Chromosomal DNA isolated from S. pombe had similar restriction patterns to those from S. cerevisiae, as determined by Southern blot analysis. The 2. 8 kb transcript of mRNA was identified by Northern hybridization. The level of transcript did not increase upon UV-irradiation, suggesting that the HRD3 gene in S. pombe is not UV-inducible.

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A New Approach to Adaptive HFC-based GAs: Comparative Study on Crossover Genetic Operator (적응 HFC 기반 유전자알고리즘의 새로운 접근: 교배 유전자 연산자의 비교연구)

  • Kim, Gil-Sung;Choi, Jeoung-Nae;Oh, Sung-Kwun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.57 no.9
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    • pp.1636-1641
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    • 2008
  • In this study, we introduce a new approach to Parallel Genetic Algorithms (PGA) which combines AHFCGA with crossover operator. As to crossover operators, we use three types of the crossover operators such as modified simple crossover(MSX), arithmetic crossover(AX), and Unimodal Normal Distribution Crossover(UNDX) for real coding. The AHFC model is given as an extended and adaptive version of HFC for parameter optimization. The migration topology of AHFC is composed of sub-populations(demes), the admission threshold levels, and admission buffer for the deme of each threshold level through succesive evolution process. In particular, UNDX is mean-centric crossover operator using multiple parents, and generates offsprings obeying a normal distribution around the center of parents. By using test functions having multimodality and/or epistasis, which are commonly used in the study of function parameter optimization, Experimental results show that AHFCGA can produce more preferable output performance result when compared to HFCGA and RCGA.

Correlative Changes between Photosynthetic Activities and Chlorophyll Fluorescence in Wheat Chloroplasts Exposed to High Temperature

  • Young-Nam Hong
    • Journal of Plant Biology
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    • v.37 no.1
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    • pp.37-42
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    • 1994
  • Correlative changes between photosynthetic O2 exchange rates and room temperature Chl fluorescence were investigated in wheat (Triticum aestivum L.) chloroplasts treated with high temperature for 5 min. With increasing treatment temperature, photosynthetic O2 evolution rate mediated by PSII was decreased, showing 50% inhibition at 38$^{\circ}C$ (I50). But PSI activity measured by O2 uptake rates was stimulated as a function of increasing temperature. Dark level fluorescence (Fo)-temperature (T) analysis showed that fluorescence rising temperature (Tr), critical temperature (Tc), and peak temperature (Tp) was 38, 43, and 52$^{\circ}C$, respectively. Quenching analysis of Chl fluorescence showed that both the oxidized fraction of plastoquinone (qQ) and degree of thylakoid membrane energization (qNP) increased up to 4$0^{\circ}C$ and then declined dramatically. These results suggest that Tr is correlated with temperature showing a 50% of inhibition of photosynthesis and under mild high temperature stress, qNP is worth regarding as indicator for heat-induced damage of photosynthesis.

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An elastoplastic bounding surface model for the cyclic undrained behaviour of saturated soft clays

  • Cheng, Xinglei;Wang, Jianhua
    • Geomechanics and Engineering
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    • v.11 no.3
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    • pp.325-343
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    • 2016
  • A total stress-based bounding surface model is developed to predict the undrained behaviour of saturated soft clays under cyclic loads based on the anisotropic hardening modulus field and bounding-surface theories. A new hardening rule is developed based on a new interpolation function of the hardening modulus that has simple mathematic expression and fewer model parameters. The evolution of hardening modulus field is described in the deviatoric stress space. It is assumed that the stress reverse points are the mapping centre points and the mapping centre moves with the variation of loading and unloading paths to describe the cyclic stress-strain hysteresis curve. In addition, by introducing a model parameter that reflects the accumulation rate and level of shear strain to the interpolation function, the cyclic shakedown and failure behaviour of soil elements with different combinations of initial and cyclic stresses can be captured. The methods to determine the model parameters using cyclic triaxial compression tests are also studied. Finally, the cyclic triaxial extension and torsional shear tests are performed. By comparing the predictions with the test results, the model can be used to describe undrained cyclic stress-strain responses of elements with different stress states for the tested clays.

Characterization of HRD3, a Schizosaccharomyces pombe Gene Involved in DNA Repair and Cell Viability

  • Choi, In-Soon
    • Animal cells and systems
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    • v.7 no.2
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    • pp.159-164
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    • 2003
  • The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. The RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA and DNA-RNA helicase activities. To examine the extent of conservation of structure and function of a S. pombe RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (homolog of RAD3 gene). Southern blot analysis confirmed that S. pombe chromosome contains the same DNA as HRD3 gene and this gene exists as a single copy in S. pombe. The transcript of 2.8 kb was detected by Northern blot analysis, The level of transcripts increased by ultraviolet (UV) irradiation, indicating that HRD3 is one of the UV-inducible genes in S. pombe. Furthermore, the predicted partial sequence of HRD3 protein has 60% identity to S. cerevisiae RAD3 gene. This homology was particularly striking in the regions identified as being conserved in a group of DNA helicases. Gene deletion experiments indicate that the HRD3 gene is essential for viability and DNA repair function. These observations suggest evolutionary conservation of other protein components with which HRD3 might interact in mediating its DNA repair and viability functions.

Modeling for Evolution of a 3-dimensional Structure on Semiconductor Substrate (반도체 기판 위의 3차원 구조에 대한 형상 진화 모델링 연구)

  • Jung, Hyun-Su;Won, Tae-Young
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.37 no.12
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    • pp.24-28
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    • 2000
  • This paper reports a new calculation method of three dimensional deposeition rate by level set method. To model an advancement of the surface efficiently, we have developed a new iteration method to re-initialize the level set function. For calculating etching and deposition rate by direct flow, we have developed a visibility test module and a refraction and re-sputtering model. Sputter deposition rate with shadow effect and surface refraction is calculated. We report that difference of profiles in cases that sticking coefficient are 1.0 and 0.3. We report that the difference of the deposition rate on bottom of the hole is caused by a difference of visible angle by the shadowing effect.

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Enzyme Kinetics Based Modeling of Respiration Rate for 'Fuyu' Persimmon (Diospyros kaki) Fruits (효소반응속도론에 기초한 단감의 호흡 모델에 관한 연구)

  • Ahn, Gwang-Hwan;Lee, Dong-Sun
    • Korean Journal of Food Science and Technology
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    • v.36 no.4
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    • pp.580-585
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    • 2004
  • Respiration of 'Fuyu' persimmon (Diospyros kaki) fruits were measured in terms of oxygen consumption rate and carbon dioxide evolution by closed system experiments at 0, 5, and $20^{\circ}C$. Enzyme kinetics-based respiration model was used to describe respiration rate as function of $O_2\;and\;CO_2$ gas concentrations $(R=V_m[O_2]/K_m+(1+[CO_2]/K_i)[O_2])$, and Arrhenius equation was applied to analyze temperature effect. $V_m\;and\;K_m$ increased, while $K_i$ decreased, with increasing temperature. $K_m\;of\;O_2$ consumption was greater than that of $CO_2$ evolution at equal temperature. Inhibitory effect of reduced $O_2$ level on $O_2$ consumption was more prominent than that on $CO_2$ evolution. Activation energy of respiration decreased with reduced $O_2$ and elevated $CO_2$ concentrations. Activation energy of $CO_2$ evolution was greater than that of $O_2$ consumption. Permeable package experiments verified respiration model parameters by showing good agreement between predicted and experimental gas concentrations in package.

A Call Admission Control Algorithm in 3GPP LTE System for Guarantee of Packet Delay (패킷 지연 보장을 위한 LTE 시스템의 호 수락 제어 알고리즘)

  • Bae, Sueng-Jae;Choi, Bum-Gon;Lee, Jin-Ju;Kwon, Sung-Oh;Chung, Min-Young
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.6A
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    • pp.458-467
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    • 2009
  • Long Tenn Evolution (LTE) is the next generation mobile phone technology which has being standardized by the Third Generation Partnership Project (3GPP). In the existing mobile communication networks, voice traffic is delivered through circuit switched networks. In LTE, however, all kinds of traffic are transferred through IP based packet switched networks which has best-effort characteristic. Therefore, providing QoS in LTE system is difficult. In order to provide QoS in LTE, RRM is very important. Especially, in part of RRM, call admission control (CAC) performs an important function to reduce network congestion and guarantee a certain level of QoS for on-going calls. In this paper, we propose a CAC algorithm in order to provide QoS for various kinds of services in LTE system. The performance of the proposed algorithm is evaluated with various simulation environments. The results show that the proposed algorithm provides QoS through rejections of requested calls. Especially, the proposed CAC algorithm can be satisfied with packet delay requirement defined in LTE specification.

An Uncertainty Assessment of Temperature and Precipitation over East Asia (동아시아 기온과 강수의 불확실성 평가)

  • Shin, Jin-Ho;Kim, Min-Ji;Lee, Hyo-Shin;Kwon, Won-Tae
    • Proceedings of the Korea Water Resources Association Conference
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    • 2008.05a
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    • pp.299-303
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    • 2008
  • In this study, an uncertainty assessment for surface air temperature(T2m) and precipitation(PCP) over East Asia is carried out. The data simulated by the intergovermental Panel on Climate Change (IPCC) Fourth Assessment Report (AR4) Atmosphere-Ocean coupled general circulation Model (AOGCM) are used to assess the uncertainty. Examination of the seasonal uncertainty of T2m and PCP variabilities shows that spring-summer cold bias and fall warm bias of T2m are found over both East Asia and the Korea peninsula. In contrast, distinctly summer dry bias and winter-spring wet bias of PCP over the Korea peninsula is found. To investigate the PCP seasonal variability over East Asia, the cyclostationary empirical orthogonal function(CSEOF) analysis is employed. The CSEOF analysis can extract physical modes (spatio-temporal patterns) and their undulation (PC time series) of PCP, showing the evolution of PCP. A comparison between spatio-temporal patterns of observed and modeled PCP anomalies shows that positive PCP anomalies located in northeastern China (north of Korea) of the multi-model ensemble(MME) cannot explain properly the contribution to summer monsoon rainfalls across Korea and Japan. The uncertainty of modeled PCP indicates that there is disagreement between observed and MME anomalies. The spatio-temporal deviation of the PCP is significantly associated with lower- and upper-level circulations. In particular, lower-level moisture transports from the warm pool of the western Pacific and corresponding moisture convergence significantly contribute to summer rainfalls. These lower- and upper-level circulations physically consistent with PCP give a insight of the reason why differences between modeled and observed PCP occur.

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LOW-LEVEL RADIO EMISSION FROM RADIO GALAXIES AND IMPLICATIONS FOR THE LARGE SCALE STRUCTURE

  • KRISHNA GOPAL;WIITA PAUL J.;BARAI PARAMITA
    • Journal of The Korean Astronomical Society
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    • v.37 no.5
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    • pp.517-525
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    • 2004
  • We present an update on our proposal that during the 'quasar era' (1.5 $\le$ z $\le$ 3), powerful radio galaxies could have played a major role in the enhanced global star-formation, and in the widespread magnetization and metal pollution of the universe. A key ingredient of this proposal is our estimate that the true cosmological evolution of the radio galaxy population is likely to be even steeper than what has been inferred from flux-limited samples of radio sources with redshift data, when an allowance is made for the inverse Compton losses on the cosmic microwave background which were much greater at higher redshifts. We thus estimate that a large fraction of the clumps of proto-galactic material within the cosmic web of filaments was probably impacted by the expanding lobes of radio galaxies during the quasar era. Some recently published observational evidence and simulations which provide support for this picture are pointed out. We also show that the inverse Compton x-ray emission from the population of radio galaxies during the quasar era, which we inferred to be largely missing from the derived radio luminosity function, is still only a small fraction of the observed soft x-ray background (XRB) and hence the limit imposed on this scenario by the XRB is not violated.