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Large scale splitter-less FFD-SPLITT fractionation: effect of flow rate and channel thickness on fractionation efficiency (대용량 중력장 SPLITT Fractionation: 분획효율에 미치는 채널 두께와 유속의 영향)

  • Yoo, Yeongsuk;Choi, Jaeyeong;Kim, Woon Jung;Eum, Chul Hun;Jung, Euo Chang;Lee, Seungho
    • Analytical Science and Technology
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    • v.27 no.1
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    • pp.34-40
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    • 2014
  • SPLITT fractionation (SF) allows continuous (and thus a preparative scale) separation of micronsized particles into two size fractions ('fraction-a' and 'fraction-b'). SF is usually carried out in a thin rectangular channel with two inlets and two outlets, which is equipped with flow stream splitters at the inlet and the outlet of the channel, respectively. A new large scale splitter-less gravitational SF (GSF) system had been assembled, which was designed to eliminate the flow stream splitters and thus is operated by the full feed depletion (FFD) mode (FFD-GSF). In the FFD mode, there is only one inlet through which the sample is fed. There is no carrier liquid fed into the channel, and thus prevents the sample dilution. The effects of the sample-feeding flow rate, the channel thickness on the fractionation efficiency (FE, number % of particles that have the size predicted by theory) of FFD-GSF was investigated using industrial polyurethane (PU) latex beads. The carrier liquid was water containing 0.1% FL-70 (particle dispersing agent) and 0.02% sodium azide (used as bactericide). The sample loading rate was varied from about 4 to 7 L/hr with the sample concentration fixed at 0.01%. The GSF channel thickness was varied from 900 to $1300{\mu}m$. Particles exiting the GSF channel were collected and monitored by optical microscopy (OM). Sample recovery was monitored by collecting the fractionated particles on a $0.45{\mu}m$ membrane filter. It was found that FE of fraction-a was increased as the channel thickness increases, and FE of fraction-b was increased as the flow rate was increased. In all cases, the sample recovery has higher than 95%. It seems the new splitter-less FFD GSF system could become a useful tool for large scale separations of various types of micron-sized particles.

Seismic Performance of Hollow Rectangular Precast Segmental Piers (프리캐스트 중공 사각형 철근콘크리트 교각의 내진성능)

  • Lee, Jae-Hoon;Park, Dong-Kyu;Choi, Jin-Ho;Shin, Sung-Jin
    • Journal of the Korea Concrete Institute
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    • v.24 no.6
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    • pp.705-714
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    • 2012
  • Precast reinforced concrete bridge columns with hollow rectangular section were tested under cyclic lateral load with constant axial force to investigate its seismic performance. After all the precast column segments were erected, longitudinal reinforcement was inserted in the sheath prefabricated in the segments, which were then mortar grouted. Main variables of the test series were column aspect ratio, longitudinal reinforcement ratio, amount of lateral reinforcement, and location of segment joints. The aspect ratios were 4.5 and 2.5, and the longitudinal steel ratios were 1.15% and 3.07%. The amount of lateral reinforcement were 95%, 55%, 50%, and 27% of the minimum amount for full ductility design requirements in the Korean Bridge Design Code. The locations of segment joints in plastic hinge region were 0.5 and 1.0 times of the section depth from the bottom column end. The test results of cracking and failure mode, axial-flexural strength, lateral load-displacement relationship, and displacement ductility are presented. Then, safety of the ductility demand based seismic design in the Korean Bridge Design Code is discussed. The column specimens showed larger ductility than expected, because buckling of longitudinal reinforcing bar was prevented due to confinement developed not only by transverse steel but also by sheath and infilling mortar.

Comuarative Evaluation on Strength of Several Grout-filled Splice Sleeve (각종 그라우트 충전식 철근이음의 내력에 대한 비교평가)

  • Kim Hyong-Kee
    • Journal of the Korea Concrete Institute
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    • v.16 no.5 s.83
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    • pp.635-643
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    • 2004
  • In this study, the tensile strength of 321 full-sized grout-filled splice steeve specimens were compared and analyzed in order to afford the data for a reasonable and economical design of this system. The experimental variables analyzed in this study were embedment length of reinforcing bars, compressive strength of grout, sleeve geometry, loading pattern and final failure mode of specimen. Following main conclusions are obtained : 1) The strength of grout-filled splice sleeve tends to be improved with increasing compressive strength of grout and embedment length of reinforcing bars. Specially this tendency appears apparent in specimens of bond failure rather than rebar failure. 2) The results of this study show that the sleeve geometry have influence on the bond strength of grout-filled splice sleeve. 3) The grout-filled splice sleeve of bond failure don't show the difference of tensile strength according to size of rebar. 4) It is verified that the tensile strength required in ACI and domestic code is retained either when the compressive strength of grout over 70 MPa is used with embedment length of reinforcing bars over 4.5d or when the compressive strength of grout over 80 MPa is used with embedment length of rebars over 3.9d. 5) It is verified that the tensile strength required in AIJ code is retained in case when the embedment length of reinforcing bars is 0.8 times the rebar diameter longer than in ACI code.

Wine quality grading by near infrared spectroscopy.

  • Dambergs, Robert G.;Kambouris, Ambrosias;Schumacher, Nathan;Francis, I. Leigh;Esler, Michael B.;Gishen, Mark
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1253-1253
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    • 2001
  • The ability to accurately assess wine quality is important during the wine making process, particularly when allocating batches of wines to styles determined by consumer requirements. Grape payments are often determined by the quality category of the wine that is produced from them. Wine quality, in terms of sensory characteristics, is normally a subjective measure, performed by experienced winemakers, wine competition judges or winetasting panellists. By nature, such assessments can be biased by individual preferences and may be subject to day-to-day variation. Taste and aroma compounds are often present in concentrations below the detection limit of near infrared (NIR) spectroscopy but the more abundant organic compounds offer potential for objective quality grading by this technique. Samples were drawn from one of Australia's major wine shows and from BRL Hardy's post-vintage wine quality allocation tastings. The samples were scanned in transmission mode with a FOSS NIR Systems 6500, over the wavelength range 400-2500 ㎚. Data analysis was performed with the Vision chemometrics package. With samples from the allocation tastings, the best correlations between NIR spectra and tasting data were obtained with dry red wines. These calibrations used loadings in the wavelengths related to anthocyanins, ethanol and possibly tannins. Anthocyanins are a group of compounds responsible for colour in red wines - restricting the wavelengths to those relating to anthocyanins produced calibrations of similar accuracy to those using the full wavelength range. This was particularly marked with Merlot, a variety that tends to have relatively lower anthocyanin levels than Cabernet Sauvignon and Shiraz. For dry white wines, calibrations appeared to be more dependent on ethanol characteristics of the spectrum, implying that quality correlated with fruit maturity. The correlations between NIR spectra and sensory data obtained using the wine show samples were less significant in general. This may be related to the fact that within most classes in the show, the samples may span vintages, glowing areas and winemaking styles, even though they may be made from only one grape variety. For dry red wines, the best calibrations were obtained with a class of Pinot Noir - a variety that tends to be produced in limited areas in Australia and would represent the least matrix variation. Good correlations were obtained with a tawny port class - these wines are sweet, fortified wines, that are aged for long periods in wooden barrels. During the ageing process Maillard browning compounds are formed and the water is lost through the barrels in preference to ethanol, producing “concentrated” darkly coloured wines with high alcohol content. These calibrations indicated heaviest loadings in the water regions of the spectrum, suggesting that “concentration” of the wines was important, whilst the visible and alcohol regions of the spectrum also featured as important factors. NIR calibrations based on sensory scores will always be difficult to obtain due to variation between individual winetasters. Nevertheless, these results warrant further investigation and may provide valuable Insight into the main parameters affecting wine quality.

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SUPPLY-DEMAND, COMMERCIAL DISTRIBUTION AND TRANSACTION OF THE CULTURED TUNA IN JAPAN - EMPHASIZING ON THE GLOBAL EXPANSION OF THE TUNA-FARMING BUSINESS -

  • Yamamoto, Naotoshi;Kameda, Kazuhiko;Nishida, Akari;Kitano, Shinichi
    • The Journal of Fisheries Business Administration
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    • v.39 no.1
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    • pp.87-114
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    • 2008
  • The cultured tuna production which has suddenly expanded at the short time and the demand for it attract attention. Farming mode, distribution transactions, change of the market (domestic and international) and the price trend are reviewed from the Japan's position which is the biggest consuming country. This paper tries to describe the current status of the food system related to the cultured tuna. Japanese government began the development of the tuna culture technology in 1970. It was by the Fisheries Agency's project. Kinki University which is the large scale private university in Japan participated in the project. After that, 32 years have passed. Kinki University established the full farming of the bluefin tuna in August, 2002. On the other hand, in 1974, one Japanese private enterprise began its tuna farming business in Canada. Kinki University gave this company technical cooperation. Also, in the early stages of the 90s, as for the policy of the overseas fishery cooperation foundation, it supported the tuna farming business in Australia. It is very clear to understand that the long-term technological-development has supported the take-off scene of the tuna culture business not only in foreign countries but also in Japan. The total shipment scale of the cultured tuna expanded very much within about 10 recent years. However, the decrease of the wild tuna catch, the reinforcement of the fisheries regulation and the tuna body to dwarf are remarkable now. Under the condition as the mentioned above, Japan's tuna consumption, especially, in the market at the fatty meat of tuna of the cultured tuna is building up firm status. At present, the Mediterranean Sea coastal countries, Australia, Mexico and Japan have the tuna farming sites. Australia farms the southern bluefin tuna. The others do the bluefin tuna. About for 3 years, Japan farms the juvenile of the tuna. The global production areas are as follows. 8 coastal countries of the Mediterranean Sea; 18,000 tons (61 % of the cultured tuna quantity in foreign countries), Mexico; 4,500 ton (15%), Australia; 7,000 tons (24%). In 2003, Japan has 32 managements and 39 offices for tuna farming. In Japan, Kyushu and Okinawa district, the share shows itself as 80 % of the domestic production quantity. Especially, the share of Amami-oshima Island in Kagoshima Prefecture exceeds 60 %. Therefore, this island has the maximum production scale of Japan. The amount of supply of BT and SBT was 56,000 tons in 2004. In Abroad, the tuna farming business forms a fixed connection between the importer and the wholesaler which have their office in Japan. In the field of the capital composition, the payment in advance, transaction and the way of settlement, each maintains their fixed relation. The market conditions of the cultured tuna are supported by "the decline of price level" and "the expansion of the general public consumption segment". These lead a team merchandising, and it is supported by the fixed business connection of each. This makes the profit of each business which are on the cultured tuna distribution. However, they have competition on the power balance among them.

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The Effects of Various Extracellular Matrices on Motility of Cultured MC3T3-E1 Cell (다양한 세포외기질이 배양 골아세포의 이동에 미치는 영향)

  • Park, Beyoung Yun;Seo, Sang Woo;Lee, Won Jai;Ryu, Chang Woo;Rah, Dong Kyun;Son, Hyun Joo;Park, Jong Chul
    • Archives of Plastic Surgery
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    • v.32 no.2
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    • pp.143-148
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    • 2005
  • Chemotactic migration of bone forming cell, osteoblast, is an important event during bone formation, bone remodeling, and fracture healing. Migration of cells is mediated by adhesion receptors, such as integrins, that link the cell to extracellular matrix ligands, type I collagen, fibronectin, laminin and depend on interaction between integrin and extracellular ligand. Our study was designed to investigate the effect of extracellular matrix like fibronectin, laminin, type I collagen on migration of osteoblast. Migration distance and speed of MC3T3-E1 cell on extracellular matrix-coated glass were measured for 24 hours using 0.01% type I collagen, 0.01% fibronectin, 100 microliter/ml laminin. The migration distance and speed of MC3T3-E1 cell was compared using a video-microscopy system. To determine migration speed, cells were viewed with a 4 phase- contrast lens and video recorded. Images were captured using a color CCD camera and saved in 8-bit full-color mode. The migration distance on 0.01% type I collagen or 0.01% fibronectin was longer than that on $100{\mu}l/ml$ laminin-coated glass. The migration speed on fibronectin-coated glass was 68 micrometer/hour which was fastest. The migration speed on type I collagen-coated glass was similar with that on fibronectin-coated glass. The latter two migration speeds were faster than that on no-coated glass. On the other hand, the average migration speed on laminin-coated glass was 37micrometer/hour and not different from that of control group. In conclusion, the extracelluar matrix ligands such as type I collagen and fibronectin seem to play an important role in cell migration. The type I collagen or fibronectin coated scaffold is more effective for migration of osteoblast in tissue engineering process.

Extraction of the ship movement information by a radar target extractor (Radar Target Extractor에 의한 선박운동정보의 추출에 관한 연구)

  • Lee, Dae-Jae;Kim, Kwang-Sik;Byun, Duck-Soo
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.38 no.3
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    • pp.249-255
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    • 2002
  • This paper describes on the extraction of ship's real-time movement information using a combination full-function ARPA radar and ECS system that displays radar images and an electronic chart together on a single PC screen. The radar target extractor(RTX) board, developed by Marine Electronics Corporation of Korea, receives radar video, trigger, antenna bearing pulse and heading pulse signals from a radar unit and processes these signals to extract target information. The target data extracted from each pulse repetition interval in DSPs of RTX that installed in 16 bit ISA slot of a IBM PC compatible computer is formatted into a series of radar target messages. These messages are then transmitted to the host PC and displayed on a single screen. The position data of target in range and azimuth direction are stored and used for determining the center of the distributed target by arithmetic averaging after the detection of the target end. In this system, the electronic chart or radar screens can be displayed separately or simulaneously and in radar mode all information of radar targets can be recorded and replayed In spite of a PC based radar system, all essential information required for safe and efficient navigation of ship can be provided.

Fast Algorithm for Disparity Estimation in ATSC-M/H based Hybrid 3DTV (ATSC-M/H 기반의 융합형 3DTV를 위한 양안시차 고속 추정 알고리즘)

  • Lee, Dong-Hee;Kim, Sung-Hoon;Lee, Jooyoung;Kang, Dongwook;Jung, Kyeong-Hoon
    • Journal of Broadcast Engineering
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    • v.19 no.4
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    • pp.521-532
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    • 2014
  • ATSC-M/H based hybrid 3DTV, which is one of the service compatible 3DTV system, has considerable quality gap between the left and right views. And CRA(Conditional Replenishment Algorithm) has been proposed to deal with the issue of resolution mismatch and improve the visual quality. In CRA, the disparity vectors of stereoscopic images are estimated. The disparity compensated left view and simply enlarged right view are compared and conditionally selected for generating the enhanced right view. In order to implement CRA, a fast algorithm is strongly required because the disparity vectors need to be obtained at every layer and the complexity of CRA is quite high. In this paper, we adopted SDSP(Small Diamond Search Pattern) instead of full search and predicted the initial position of search pattern by examining the spatio-temporal correlation of disparity vectors and also suggested the SKIP mode to limit the number of processing units. The computer simulation showed that the proposed fast algorithm could greatly reduce the processing time while minimizing the quality degradation of reconstructed right view.

Epizootiological Study of Salmonella Infection on Piggery: I. Study on Distribution, Occurrence, Serovars and Biovars (양돈장(養豚場)에 있어서 Salmonella감염증(感染症)의 역학적(疫學的) 연구(硏究) : I. 발생(發生) 및 오염상황(汚染狀況), 혈청형(血淸型)과 Salmonella typhimurium의 생물형(生物型))

  • Choi, Won-pil;Lee, Hi-suk;Yeo, San-geon;Lee, Hun-jun;Jung, Suk-chan
    • Korean Journal of Veterinary Research
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    • v.26 no.1
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    • pp.49-59
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    • 1986
  • This paper deals with the distribution, reservoir and mode of spread of salmonella infection on 7 pig farms in Taegu, Gyeongbuk, Gyeongnam and Chungnam and a slaughter house in Teagu during the period from May 1984 to May 1985. Isolated salmonella were examined for serotypes and biotyping of S. typhimurium. The results obtained were summarised as follows; 1. Of total 7,995 samples from 7 pig farms and a slaughter house, 319 salmonella were isolated from 234 samples (2.9%) and their serovar strains were S. derby 77, S. infantis 41, S. enteritidis 20, S. typhimurium 18, S. bredeney 16, S. london 14, S. paratyphi B 9, S. anatum 8, S. montevideo 8, S. senftenberg 7, S. thompson 6, S. pullorum 4, S. paratyphi A 1 and untypable 70. 2. The incidence rate of diarrhea of piglets, weaned pigs and fattening pigs was 9.8%, 2.3% and 0.5%, respectively whereas the rate by salmonella infection was 4.2%, 1.2% and 11.3%, respectively. 3. The isolation rate of salmonella was higher in summer and autumn. 4. The isolation rate of salmonella varied from 1.1% to 4.5% in 7 pig farms, it was higher in sewages(4.4%), weaned pigs(3.7%), boars(3.7%) and other(3.7%) included soils, manure and wild rats according to samples. Three out of 7 pig farms were contaminated heavily with various serovars of salmonella. 5. The isolation rate of salmonella from pigs slaughtered was 8.1%, it was 13.6% in rectal contents and 1.6% in mensenteric lymph nodes. 6. Eighteen strains of S. typhimurium were classified into 3 different biovars(1, 10 and 10a) by the method of Brandis and were subdivided into 6 different full biovars(1a, 1d, 1dh, 3d, 26i and 26ei) by the method described by Duguid et al. Appearance of different biovars indicated the occurrence of different exotic infection sources on the farms.

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Signal-Characteristic Analysis with Respect to Backing Material of PVDF-Based High-Frequency Ultrasound for Photoacoustic Microscopy (광음향 현미경을 위한 PVDF 기반 고주파수 초음파 변환기의 흡음층 소재에 따른 신호 특성 분석)

  • Lee, Junsu;Chang, Jin Ho
    • Journal of the Korean Society for Nondestructive Testing
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    • v.35 no.2
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    • pp.112-119
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    • 2015
  • Photoacoustic microscopy is capable of providing high-resolution molecular images, and its spatial resolution is typically determined by ultrasonic transducers used to receive the photoacoustic signals. Therefore, ultrasonic transducers for photoacoustic microscopy (PAM) should have a high operating frequency, broad bandwidth, and high signal-reception efficiency. Polyvinylidene fluoride (PVDF) is a suitable material. To take full advantage of this material, the selection of the backing material is crucial, as it influences the center frequency and bandwidth of the transducer. Therefore, we experimentally determined the most suitable backing material among EPO-TEK 301, E-Solder 3022, and RTV. For this, three PVDF high-frequency single-element transducers were fabricated with each backing material. The center frequency and -6 dB bandwidth of each transducer were ascertained by a pulse-echo test. The spatial resolution of each transducer was examined using wire-target images. The experimental results indicated that EPO-TEK 301 is the most suitable backing material for a PAM transducer. This material provides the highest signal magnitude and a reasonable bandwidth because a large portion of the energy propagates toward the front medium, and the PVDF resonates in the half-wave mode.