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Molecular cloning, expression and characterization of a squalene synthase gene from grain amaranth (Amaranthus cruentus L.)

  • Park, Young-Jun;Nemoto, Kazuhiro;Matsushima, Kenichi;Um, Han-Yong;Choi, Jung-Hoon;Oh, Chan-sung;Nishikawa, Tomotaro
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.89-89
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    • 2017
  • A gene encoding squalene synthase from grain amaranth was cloned and characterized. The full-length cDNA was 1805-bp long and contained a 1248-bp open reading frame encoding a protein of 416 amino acids with a molecular mass of 47.6 kDa. Southern blot analysis revealed that the A. cruentus genome contained a single copy of the gene. Comparison of the cDNA and genomic sequences indicated that the amaranth SQS gene had 12 introns and 13 exons. All of the exons contributed to the coding sequence. The predicted amino acid sequence of the SQS cDNA shared high homology with those of SQSs from several other plants. It contained conserved six domains that are believed to represent crucial regions of the active site. We conducted qRT-PCR analyses to examine the expression pattern of the SQS gene in seeds at different developmental stages and in several tissues. The amaranth SQS gene was low levels of SQS transcripts at the initial stage of seed development, but the levels increased rapidly at the mid-late developmental stages before declining at the late developmental stage. These findings showed that the amaranth SQS is a late-expressed gene that is rapidly expressed at the mid-late stage of seed development. In addition, we observed that the SQS mRNA levels in stems and roots increased rapidly during the four- to six-leaf stage of development. Therefore, our results showed that the expression levels of SQS in stem and root tissues are significantly higher than those in leaf tissues. In present study provides useful information about the molecular characterization of the SQS clone isolated from grain amaranth. Finally, a basic understanding of these characteristics will contribute to further studies on the amaranth SQS.

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A Diamond Web-grid Search Algorithm Combined with Efficient Stationary Block Skip Method for H.264/AVC Motion Estimation (H.264/AVC 움직임 추정을 위한 효율적인 정적 블록 스킵 방법과 결합된 다이아몬드 웹 격자 탐색 알고리즘)

  • Jeong, Chang-Uk;Choi, Jin-Ku;Ikenaga, Takeshi;Goto, Satoshi
    • Journal of Internet Computing and Services
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    • v.11 no.2
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    • pp.49-60
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    • 2010
  • H.264/AVC offers a better encoding efficiency than conventional video standards by adopting many new encoding techniques. However, the advanced coding techniques also add to the overall complexity for H.264/AVC encoder. Accordingly, it is necessary to perform optimization to alleviate the level of complexity for the video encoder. The amount of computation for motion estimation is of particular importance. In this paper, we propose a diamond web-grid search algorithm combined with efficient stationary block skip method which employs full diamond and dodecagon search patterns, and the variable thresholds are used for performing an effective skip of stationary blocks. The experimental results indicate that the proposed technique reduces the computations of the unsymmetrical-cross multi-hexagon-grid search algorithm by up to 12% while maintaining a similar PSNR performance.

The Problem and Challenges of Pest Control in Korea (우리나라의 쥐 및 위생해충 방제에 대한 문제점과 개선방향)

  • 전순표
    • Journal of environmental and Sanitary engineering
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    • v.7 no.2
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    • pp.129-140
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    • 1992
  • Rats and Cockroaches are the most problematic pests of our living environment, Rats generally fall under three categories, namely, the Norway rats, the roof rats and the mouses, Among these three types, the roof rats are increasing rapidly in number and their habitats are spreading quickly in the city area, where as the Norway rats are decreasing both in number and habitat. There are numerous damages that these pests create in our society, The rat induce damages to computers and other information processing equipments, sometimes cause light-out, and other financial losses and other sanitary problems. The cockroaches, apart from inducing serious sanitary problems, is an insect that creates insectophobia. decently, it has been revealed that cockroaches are also the main carriers of allergic diseases. Among the pesticides employed against rats in Korea, Beta - fluoro acetate(1080) was initially used after the National Liberalization. Later, the Korean government imported anticoagulant rodenticide for its control. This was followed by a brief, unsuccessful utilization of Zinc Phosphide. Since the begin of 1980s, with the advent of pest control companies, first generation anticoagulant rodenticide were developed in Korea and recently followed by a widespread use of pesticide that utilizes second generation anticoagulant material, the "Brodifacoum" . Among insecticides employed against cockroaches, "Fenitrothion" "Dusban" "DDVP" "Diazinon" and others have been used. They were gradually being replaced by the use of synthesised pyrethroid insecticides until recently. Today there is a wide spread use of bait poisons, the "hydramethylnon" Nevertheless, full prevention and control can not be realized with the sole use of the pesticides. In the future, hygine and proofing, combined with affective pest management, must be incorporated in order to bring about more satisfactory and fruitful preventive measures.

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Schwannoma of the Orbit

  • Kim, Kwang Seog;Jung, Jin Woo;Yoon, Kyung Chul;Kwon, Yu Jin;Hwang, Jae Ha;Lee, Sam Yong
    • Archives of Craniofacial Surgery
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    • v.16 no.2
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    • pp.67-72
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    • 2015
  • Background: A schwannoma is a benign, slow-growing peripheral nerve sheath tumor that originates from Schwann cells. Orbital schwannomas are rare, accounting for only 1% of all orbital neoplasms. In this study, we retrospectively review orbital schwannomas and characterize clinical, radiologic, and histologic features of this rare entity. Methods: A retrospective review was performed to identify patients with histologically confirmed orbital schwannoma, among a list of 437 patients who had visited our hospital with soft tissue masses within the orbit as the primary presentation between 2010 and 2014. Patient charts and medical records were reviewed for demographic information, relevant medical and family history, physical examination findings relating to ocular and extraocular sensorimotor function, operative details, postoperative complications, pathologic report, and recurrence. Results: Five patients (5/437, 1.1%) were identified as having histologically confirmed orbital schwannoma and underwent complete excision. Both computed tomography (CT) and magnetic resonance imaging (MRI) studies were not consistent in predicting histologic diagnosis. There were no complications, and none of the patients experienced significant scar formation. In two cases, patients exhibited a mild postoperative numbness of the forehead, but the patients demonstrated full recovery of sensation within 3 months after the operation. None of the five patients have experienced recurrence. Conclusion: Orbital schwannomas are relatively rare tumors. Preoperative diagnosis is difficult because of its variable presentation and location. Appropriate early assessment of orbital tumors by CT or MRI and prompt management is warranted to prevent the development of severe complications. Therefore, orbital schwannomas should be considered in the differential diagnosis of slow-growing orbital masses.

Gyro-Mouse for the Disabled: 'Click' and 'Position' Control of the Mouse Cursor

  • Eom, Gwang-Moon;Kim, Kyeong-Seop;Kim, Chul-Seung;Lee, James;Chung, Soon-Cheol;Lee, Bong-Soo;Higa, Hiroki;Furuse, Norio;Futami, Ryoko;Watanabe, Takashi
    • International Journal of Control, Automation, and Systems
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    • v.5 no.2
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    • pp.147-154
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    • 2007
  • This paper describes a 'gyro-mouse', which provides a new human-computer interface (HCI) for persons who are disabled in their upper extremities, for handling the mouse-click and mouse-move function. We adopted the artificial neural network to recognize a quick-nodding pattern of the disabled person as the gyro-mouse click. The performance of our gyro-mouse was evaluated by three indices that include 'click recognition rate', 'error in cursor position control', and 'click rate per minute' on a target box appearing at random positions. Although it turned out that the average error in cursor positioning control was 1.4-1.5 times larger than that of optical mouse control, and the average click rate per minute was 40% of the optical mouse, the overall click recognition rate was 93%. Moreover, the click rate per minute increased from 35.2% to 44% with repetitive trials. Hence, our suggested gyro-mouse system can be used to provide a new user interface tool especially for those persons who do not have full use of their upper extremities.

Fast motion estimation scheme based on Successive Elimination Algorithm for applying to H.264 (H.264에 적용을 위한 SEA기반 고속 움직임 탐색 기법)

  • Lim Chan;Kim Young-Moon;Lee Jae-Eun;Kang Hyun-Soo
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.42 no.2 s.302
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    • pp.151-160
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    • 2005
  • In this paper, we propose a new fast motion estimation algorithm based on successive elimination algorithm (SEA) which can dramatically reduce heavy complexity of the variable block size motion estimation in H.264 encoder. The proposed method applies the conventional SEA in the hierarchical manner to the seven block modes. That is, the proposed algorithm can remove the unnecessary computation of SAD by means of the process that the previous minimum SAD is compared to a current SAD for each mode which is obtained by accumulating sum norms or SAD of $4\times4$ blocks. As a result, we have tighter bound in the inequality between SAD and sum norm than in the ordinary SEA. If the basic size of the block is smaller than $4\times4$, the bound will become tighter but it also causes to increase computational complexity, specifically addition operations for sum norm. Compared with fast full search algorithm of JM of H.264, our algorithm saves 60 to $70\%$ of computation on average for several image sequences.

A fast block-matching algorithm using the slice-competition method (슬라이스 경쟁 방식을 이용한 고속 블럭 정합 알고리즘)

  • Jeong, Yeong-Hun;Kim, Jae-Ho
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.38 no.6
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    • pp.692-702
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    • 2001
  • In this paper, a new block-matching algorithm for standard video encoder is proposed. The algorithm finds a motion vector using the increasing SAD transition curve for each predefined candidates, not a coarse-to-fine approach as a conventional method. To remove low-probability candidates at the early stage of accumulation, a dispersed accumulation matrix is also proposed. This matrix guarantees high-linearity to the SAD transition curve. Therefore, base on this method, we present a new fast block-matching algorithm with the slice competition technique. The Candidate Selection Step and the Candidate Competition Step makes an out-performance model that considerably reduces computational power and not to be trapped into local minima. The computational power is reduced by 10%~70% than that of the conventional BMAs. Regarding computational time, an 18%~35% reduction was achieved by the proposed algorithm. Finally, the average MAD is always low in various bit-streams. The results were also very similar to the MAD of the full search block-matching algorithm.

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A Study on the Fabrication of 1W Power Amplifier for IMT2000 Repeater Using Nonlinear Analysis (비선형 해석법을 이용한 IMT2000 중계기용 1W 전력증폭기 제작 연구)

  • 전광일
    • Journal of the Institute of Electronics Engineers of Korea TE
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    • v.37 no.2
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    • pp.83-90
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    • 2000
  • A simple low-cost and small size 1.88-198 GHz Band RF power amplifier module is developed for IMT2000 repeater. The power amplifier consists of two stage amplifiers that the first stage amplifier is drive amplifier using discrete type P-HEMT (ATF-34143, 800 micron gate width, Agilent Technologies) and the second is power amplifier with 300Bm 1dB gain compression point using GaAs FET(EFA240D-SOT89, 2400 micron gate width, Excelics Semiconductor). this power amplifier module feature a 29.5dBm 1dB gain compression point, 29.5dB gain, 42dBm 3rd order intercept point(OIP3) and -10dB/-l2dB input/output return loss over the 1880-1980 MHz. This PA module is fully integrated using MIC technology into a small size and design by full nonlinear design technologies. The dimensions of this PA module are 42(L) $\times$ 34(W) mm.

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Quantitative Analysis of Phosphors Decay Characteristic for Flicker-free Display System Design (플리커 저감 디스플레이 시스템 설계를 위한 인광체 잔광 특성의 정량적 해석)

  • Kwon, Yong-Dae;Choi, Duk-Kyu;Han, Chan-Ho;Lee, Gwang-Soon;Kim, Eun-Su;Lee, Sang-Hoon;Sohng, Kyu-Ik
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.37 no.6
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    • pp.9-16
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    • 2000
  • The perception of flicker on the computer display devices depends upon the temporal waveform of the phosphor decay characteristic, the frame rate, and the display size. The lowest frequency at which flicker is not perceived is called the critical fusion frequency or critical frequency. Critical fusion frequency is evaluated by the display illuminance and the modulation (m) defined as the ratio of the amplitude of first harmonic frequency to the DC of the waveform. In this paper, we analyze the relationship bet ween the critical fusion frequency, relating to the decay characteristic of the phosphors and luminance on the monitor, and the frame frequency. Also under considering the viewing angle, we presented the frame frequency that is less sensitive to the full size of the display device.

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Emulated Vision Tester for Automatic Functional Inspection of LCD Drive Module PCB (LCD 구동 모듈 PCB의 자동 기능 검사를 위한 Emulated Vision Tester)

  • Joo, Young-Bok;Han, Chan-Ho;Park, Kil-Houm;Huh, Kyung-Moo
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.46 no.2
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    • pp.22-27
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    • 2009
  • In this paper, an automatic functional inspection system EVT (Emulated Vision Tester) for LCD drive module PCB has been proposed and implemented. Typical automatic inspection system such as probing methods and vision-based systems are widely known and used, however, there exist undetectable defects due to critical timing factors which they may miss to catch from LCD equipments. Especially typical vision-based systems have inconsistency on acquisition of images so that distinction between gray scales can be difficult which results in low level of performance and reliability on the inspection results. The proposed EVT system is pure hardware solution. It directly compares pattern signals from a pattern generator to output signals from LCD drive module. It also inspects variety of analog signals such as voltage, resistance, wave forms and so forth. The EVT system not only shows high performance in terms of reliability and processing speed but reduces costs on inspection and maintenance. Also, full automation of entire production line can be realized when EVT is applied in in-line inspection processes.