• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.5
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• pp.432-443
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• 2005

Microarray technology has contributed Significantly to the understanding of bacterial genetics and transcriptional regulation. One neglected aspect of this technology has been optimization of microarray-generated signals and quality of generated information. Full genome microarrays were developed for Clostridium acetobutylicum through spotting of PCR products that were designed with minimal homology with all other genes within the genome. Using statistical analyses it is demonstrated that Signal quality is significantly improved by increasing the hybridization volume. possibly increasing the effective number of transcripts available to bind to a given spot, while changes in labeled probe amounts were found to be less sensitive to improving signal quality. In addition to Q-RT-PCR, array validation was tested by examining the transcriptional program of a mutant (M5) strain lacking the pSOL1 178-gene megaplasmid relative to the wildtype (WT) strain. Under optimal conditions, it is demonstrated that the fraction of false positive genes is 1% when considering differentially expressed genes and 7% when considering all genes with signal above background. To enhance genomic-scale understanding of organismal physiology, using data from these microarrays we estimated that $40{\sim}55%$ of the C. acetobutylicum genome is expressed at any time during batch culture, similar to estimates made for Bacillus subtilis.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.8
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• pp.1138-1144
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• 2002

Recently GDI(Gasoline Direct Injection) engine is spot-lighted to achieve higher thermal efficiency under partial loads and better performance at full loads. To realize this system, it is essential to make both stratified combustion and homogeneous combustion. Spray pattern must be optimized according to injection timing because ambient pressure in combustion chamber is varied with crank angle. In this experimental study, two types of visualization system such as laser scattering method and schlieren method were developed to clarity the spray behavior during on intake stroke. As the ambient pressure increases, thepenetration length and spray angle show a tendancy to decrease due to rising resistance caused by the drag force of the ambient air. Distribution of injected fuel on intake stroke has a significant effect on homogeneous mixture in the cylinder. These results provide the information on macroscopic wall-wet growth in the cylinder and design factors for developing GDI injector.

• Transactions of the Korean Society of Automotive Engineers
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• v.9 no.5
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• pp.89-95
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• 2001

Recently GDI(Gasoline Direct Injection) engine is spotlighted to achieve higher thermal efficiency under partial loads and better performance at full loads. To realize this system, it is essential to make both stratified combustion and homogeneous combustion. When compared to PFI(Port Fuel Injection) engine, GDI engine needs more complicated control and optimal design with injection system. In addition, spray pattern must be optimized according to injection timing because ambient pressure in combustion chamber is also varied. Thus spray structure should be analyzed in details to meet various conditions. In this experimental study, two types of visualization system were developed to simulate compression stroke and intake stroke, respectively. With an increase of the ambient pressure, the penetration length tends to decrease due to rising resistance caused by the drag force of the ambient air. Spray characteristics impinged on the piston has a significant effect on mixture stratification around the spark plug. These results provide the information on macroscopic spray structure and design factors far developing GDI injector.

• 한국신재생에너지학회:학술대회논문집
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• 2005.06a
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• pp.243-247
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• 2005

This paper describes an on-going national R&D program for the development of a gas turbine/fuel cell hybrid power generation system and related R&D activities. The final goal of this program is to develop a 200kW-c1ass gas turbine/fuel cell hybrid power generation system and achieve high efficiency over $60\%$ (AC/LHV). In the first phase of the development, a sub-scaled 60kW-class hybrid system based on the 50kW-class microturbine and the 5kW SOFC will be developed for the purpose of concept proof of the hybrid system. Core components such as the microturbine and the SOFC system are being developed and parallel preparation for system integration is being carried out. Before the core components are assembled in the final system. operating characteristics of a hybrid system are investigated from a simulated system where a turbocharger (microturbine simulator) and a modified fuel cell burner test facility (fuel cell simulator) are employed. The 60kW demonstration unit will be built up and operated to provide the valuable information for the preparation of the final full scale 200kW hybrid system.

• International Journal of Quality Innovation
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• v.5 no.1
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• pp.34-42
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• 2004

In the traditional use of conjoint analysis, in order to evaluate the relative importance of several elements composing a service, interviewed customers are asked to express their judgement about different scenarios (specific combinations of elements). In order to reduce the number of possible scenarios, design of experiments methodology is usually exploited. Previous experiences show that, even a limited number of proposed scenarios cause difficulty in answering for the interviewed customer if the scenarios differ for elements of very low interest to him/her. Consequently, a high rate of abandon of the interview has been observed. In this study it is assumed that a service can be decomposed in several improvable elements and/or enriched with new "optionals". In both cases, what under study is assumed to be a set of dichotomous attributes. For each of these attributes, its marginal contribution to customer satisfaction has to be modelled and estimated. To obtain the required information, an opportune questionnaire is proposed to a sample of interviewed customers. An interviewing procedure consisting in a customer driven design of scenarios is followed, starting from the full-optional scenario and eliminating one by one the less satisfying elements. For each interviewed customer, a ranking of attributes is so obtained. Then, by asking the interviewed customer to evaluate on a metric scale the scenarios he previously selected, a rating of attributes can also be obtained. A case study conducted in collaboration with a public transportation company is presented. Contrarily to previous experiences, the abandon rate proved extremely reduced.y reduced.

• Parasites, Hosts and Diseases
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• v.54 no.3
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• pp.369-373
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• 2016

The 2 species of the genus Anoplocephala (Anoplocephalidae), A. perfoliata and A. magna, are among the most important equine cestode parasites. However, there is little information about their differences at the molecular level. The present study revealed that the mitochondrial (mt) genome of A. magna was 13,759 bp in size and 700 bp shorter than that of A. perfoliata. The 2 species includes 2 rRNA, 22 tRNA, and 12 protein-coding genes each. The size of each of the 36 genes was the same as that of A. perfoliata, except for cox1, rrnL, trnC, trnS2(UCN), trnG, trnH, trnQ, and trnP. In the full mitochondrial genome, the sequence similarity was 87.1%. The divergence in the nucleotide and amino acid sequences of individual protein-coding genes ranged from 11.1% to 16% and 6.8% to 16.4%, respectively. The 2 non-coding regions of the mt genome of A. magna were 199 bp and 271 bp in length, while the equivalent regions in A. perfoliata were 875 bp and 276 bp, respectively. The results of this study support the proposal that A. magna and A. perfoliata are separate species, consistent with previous morphological analyses.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.581-584
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• 2005

A district heating(DH) system supplies environmentally-friend heat and is appropriate for reduction of energy consumption and/or air pollutions. The objectives of this paper are to systematize data processing of transition temperature, investigate its effects on fatigue life of DH pipes and optimization for size of DH pipes. A relational database management system as well as reliable fatigue life evaluation procedures is established for Korean DH pipes. Also, since the prototypal evaluation results satisfied both cycle-based and stress-based fatigue criteria. Through the optimum design process, the cross section diminished 18.64% and the CUF diminished 23.35%. So, it can be used as useful information in the future for optimal design, operation and energy saving via setting of efficient condition and stabilization of water temperature.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.1095-1098
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• 2005

Most electronic chassis control systems until today have been designed with optimization on its own performance. However, According to the increase of the interest regarding a vehicle safety and development of information technique, the integration technique of current chassis systems is being emphasized. Each enterprise proposed it with name of GCC(Global Chassis Control) or UCC(Unified Chassis Control). This study realizes control algorithm of suspension and brake by using the vehicle model of low degree of freedom as the primary stage of realization of integrated chassis control system. The proposed algorithm build the simulation environment connected to the CarSim having full vehicle model of 27 degree of freedom for raising the thrust of results

• Transactions of Materials Processing
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• v.9 no.3
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• pp.233-241
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• 2000

It is well known that the quality and efficiency of the design of metal forming processes can be significantly improved with the aid of effective numerical simulations. In the present study, a two-and three-dimensional finite element simulation system, CAMP form, was developed for the analysis of metal forming processes in the PC environment. It is composed of a solver based on the thermo-rigid-viscoplastic approach and graphic user interface (GUI) based pre-and post-processors to be used for the effective description of forming conditions and graphic display of simulation results, respectively. In particular, in the case of CAMPform 2D (two-dimensional), as the solver contains an automatic remeshing module which determines the deformation step when remeshing is required and reconstructs the new mesh system, it is possible to carry out simulations automatically without any user intervention. Also, the forming analysis considers ductile fracture of the workpiece and wear of dies for better usage of the system. In the case of CAMPform 3D, general three-dimensional problems that involve complex die geometries and require remeshing can be analyzed, but full automation of simulations has yet to be achieved. In this paper, the overall structure and computational background of CAMPform will be briefly explained and analysis results of several forming processes will be shown. From the current results, it is construed that CAMPform can be used in providing useful information to assist the design of forming processes.

• Proceedings of the Korean Nuclear Society Conference
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• 1995.10a
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• pp.185-190
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• 1995

Conventional alarm system has many difficulties in the operator's identifying the plant status during special situations such as design basis accidents. To solve the shortcomings, an on-line alarm annunciator system, called dynamic alarm console (DAC), was developed. In the DAC, a signal is generated as alarm by the use of an adaptive setpoint check strategy based on operating mode, and time delay technique is used not to generate nuisance alarms. After alarm generation, if activated alarm is a level precursor alarm or a consequencial alarm, it would be suppressed, and the residual alarms go through dynamic prioritization which provide the alarms with pertinent priorities to the current operating mode. Dynamic prioritization is achieved by going through the system- and mode-oriented prioritization. The DAC has the alarm hierarchical structure based on the physical and functional importance of alarms. Therefore the operator can perceive alarm impacts on the safety or performance of the plant with the alarm propagation from equipment level to plant functional level. In order to provide the operator with the most possible cause of the event and quick cognition of the plant status even without recognizing the individual alarms, reactor trip status tree (RTST) was developed. The DAC and the RTST have been simulated with on-line data obtained from the full-scope simulator for several abnormal cases. The results indicated that the system can provide the operator with useful and compact information fur the earlier termination and mitigation of an abnormal state.