• Journal of Communications and Networks
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• 제18권2호
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• pp.190-200
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• 2016

In this paper, we consider some aspects of binding properties that bind an anonymous user with messages. According to whether all the messages or some part of the messages are bound with an anonymous user, the protocol is said to satisfy the full binding property or the partial binding property, respectively. We propose methods to combine binding properties and anonymity-based authenticated key agreement protocols. Our protocol with the full binding property guarantees that while no participant's identity is revealed, a participant completes a key agreement protocol confirming that all the received messages came from the other participant. Our main idea is to use an anonymous signature scheme with a signer-controlled yet partially enforced linkability. Our protocols can be modified to provide additional properties, such as revocable anonymity. We formally prove that the constructed protocols are secure.

• BMB Reports
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• 제39권6호
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• pp.686-695
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• 2006

Interleukin-2 enhancer binding factor 2 (ILF2) was reported to regulate transcription of interleukin-2 (IL-2), a central cytokine in the regulation of T-cell responses. This property of ILF2 was well characterized in human and mammals, but little is known in bony fish. In this paper, an ILF2 homologue was cloned and well characterized from Tetraodon nigrovirid is for the further investigation of the function of ILF2 in bony fish. The full-length Tetraodon ILF2 cDNA was 1380 bp in size and contained an open reading frame (ORF) of 1164 bp that translates into a 387 amino-acid peptide with a molecular weight of 42.9 kDa, a 5' untranslated region (UTR) of 57 bp, and a 3' UTR of 159 bp containing a poly A tail. The deduced peptide of Tetraodon ILF2 shared an overall identity of 58%~93% with other known ILF2 sequences, and contained two N-glycosylation sites, two N-myristoylation sites, one RGD cell attachment sequence, six protein kinase C phosphorylation sites, one amino-terminal RGG-rich single-stranded RNA-binding domain, and a DZF zinc-finger nucleic acid binding domain, most of which were highly conserved through species compared. Constitutive expression of Tetraodon ILF2 was observed in all tissues examined, including gill, gut, head kidney, spleen, liver, brain and heart. The highest expression was detected in heart, followed by liver, head kidney and brain. Stimulation with LPS did not significantly alter the expression of Tetraodon ILF2. Gene organization analysis showed that the Tetraodon ILF2 gene have fifteen exons, one more than other known ILF2 genes in human and mouse. Genes up- and down-stream from the Tetraodon ILF2 were Rpa12, Peroxin-11b, Smad4, Snapap and Txnip homologue, which were different from that in human and mouse.

• BMB Reports
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• 제49권9호
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• pp.497-501
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• 2016

Wide-line ¹H NMR intensity and differential scanning calorimetry measurements were carried out on the intrinsically disordered 73-residue full transactivation domain (TAD) of the p53 tumor suppressor protein and two peptides: one a wild type p53 TAD peptide with a helix pre-structuring property, and a mutant peptide with a disabled helix-forming propensity. Measurements were carried out in order to characterize their water and ion binding characteristics. By quantifying the number of hydrate water molecules, we provide a microscopic description for the interactions of water with a wild-type p53 TAD and two p53 TAD peptides. The results provide direct evidence that intrinsically disordered proteins (IDPs) and a less structured peptide not only have a higher hydration capacity than globular proteins, but are also able to bind a larger amount of charged solute ions.

• 한국식품영양학회지
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• 제36권4호
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• pp.264-273
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• 2023

Physicochemical properties and storage stability of plant-based alternative meat prepared with low-fat soybean powder (LPAM) treated by supercritical-CO₂ and those of full-fat soybean powder (FPAM) were compared. Ash and crude protein contents were higher in LPAM than in FRAM. Water absorption capacity and oil absorption capacity were significantly higher in LPAM than in FPAM. Water binding capacity was higher in LPAM than in FPAM during a 20 days storage period at 5℃ and pH was significantly lower in LPAM than in FPAM after a 5~10 days storage period. Hardness, gumminess and chewiness significantly increased with the increase in the storage period, and the three were significantly higher in LPAM than in FPAM after 10 days and 20 days of storage. The acid value showed no remarkable difference according to the storage period in LPAM; however, it was significantly higher in FPAM than in LPAM after 20 days of storage. The peroxide value and TBA value were significantly increased according to the storage period, and were significantly lower iin LPAM than in FPAM during all the storage periods. Therefore, the use of low-fat soybean powder may be effective in improving oxidative stability during storage in the production of plant-based alternative meat.

• 한국중재학회지:중재연구
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• 제15권1호
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• pp.113-146
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• 2005

Bankruptcy proceedings serve the purpose of the collective satisfaction of the debtor's creditors through the realisation of the debtor's assets and the distribution of the proceeds therefrom. Upon the adjudication bankruptcy, the debtor's right to administer and dispose of the property belonging to the bankruptcy estate shall be vested in the administrator. If a mutual contract was not or not completely fulfilled by the debtor and the other party at the time of the adjudication of bankruptcy, the administrator has right to choose wether to fulfil or terminate the contractual relation. Legal acts that have been conducted prior to the adjudication of bankruptcy and that are detrimental to the debtor's creditors may be contested by the administrator. However, these effects of bankruptcy will have not great influence on the arbitration agreement between the debtor and another party. An arbitration agreement that has been conducted prior to the adjudication of bankruptcy is binding the administrator as an universal legal successor of debtor. Only the arbitration agreement directly disadvantageous to the debtor's creditors may be contested by the administrator. Furthermore, it is not at the discretion of administrator whether or not to submit the dispute to arbitration because an arbitration agreement does not belong under the category of Art. 50 Korean bankruptcy Act which demands a mutual contract. Arbitral proceeding upon the property of the bankruptcy estate and pending for the debtor as plaintiff or against the debtor as defendant at the date of the adjudication of bankruptcy may be taken up at the given status by the administrator. This leads to a change of the party. If a duly summoned party fails to appear in arbitration court, the arbitrator, if satisfied there is no valid excuse, may continue the proceedings and make the award as if all the parties were present. This may be disadvantagious to the debtor's creditors because the arbitral award have the same effects on the participants as the final and conclusive judgement of the court. Even if there is a change of party on side of debtor to the administrator in bankruptcy, the arbitral proceedings will not be automatically postponed or suspended. The matter of how to proceed is at discretion of administrator, when the parties haven't agree on the arbitral proceedings. He can continue the arbitral proceedings without to grant an adjournment of hearing. However, an arbitration award may be challenged by a party dissatisfied and set aside by the court based upon the misconduct that violates the basic rights of the parties to a fair hearing. The arbitrator must treat the parties equally in the arbitral proceedings and give each party a full opportunity to present his case. The arbitrator, therefore, will carefully exercise his discretion in determining whether to continue the arbitral proceedings or to grant a postponing. In the practice, the arbitral proceedings may be usually postponed to grant due process.

• 미생물학회지
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• 제38권3호
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• pp.153-161
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• 2002

Sox4는 DNA 결합 도메인인 HMG-box와 전사 활성 도메인인 serine rich region (SRR)과 아직 그 기능이 알려져 있지 않은 glycine rich region (GRR)등의 세 개의 기능 도메인을 가지는 전사인자이다. 전사인자인 Sox4는 생체 내 초기 분화 시 중요한 역할을 하는 유전자로 알려져 있으나 여전히 그 정확한 생리적 기능 및 세포 내에서 이 유전자 산물이 전사 활성 에 어떻게 관여하는지 그 정확한 기전은 잘 밝혀져 있지 않다. 이에 본 연구에서는 Sox4의 생리적 기능을 이해하고 세포 내에서 Sox4의 기능 연구에 유용하게 사용할 수 있는 항체를 제조하기 위해 Sox4를 대장균에서 발현, 정제하였다. 모든 기능 도메인을 다 포함하는 Sox4는 대장균에서 발현 시 대부분 절단되는 양상을 나타내었다. Sox4의 각 도메인을 대장균에서 GST-융합 단백질로 발현, 정제하여 그 발현 양상을 비교해 본 바 N-말단을 제거한 Sox4 ($\Delta$HMG)의 경우 67 kDa 크기의 단백질이 생성되므로 이 단백질을 항원으로 이용하여 Sox4의 GRR에 특이적으로 반응하는 항체를 제조하였다. 또한, 67 kDa 크기의 단백질 외에 34 kDa 크기에서 GST-융합 단백질이 관찰되었다. 이 밴드는 Sox4 (GRR)를 발현, 정제시에도 관찰되는 동일한 크기의 밴드이며 thrombin과의 반응을 통해 7 kDa 크기로 절단되는 Sox4 밴드이다. 그러므로 이 들 결과로부터 GRR 내에 단백질 분해효소의 표적 부위가 존재함을 확인할 수 있었다. 본 연구결과는 아직 그 기능이 밝혀져 있는 않은 Sox4의 새로운 도메인인 GRR이 단백질 분해 효소의 표적 부위로 작용하여 Sox4의 안정성을 조절함으로써 Sox4의 활성에 중요한 역할을 수행할 수 있다는 가능성을 제시하고 있다.은 억제 회복 효과는 $Mg^{2+}$에 의한 리보자임의 td intron 구조적 안정성에 기인하는 것으로 추정된다.력이 뛰어난(adaptable) 인간적 요구사항을 충족시켜야 한다. 셋째, 다이내믹 시미트리는 역(逆, reciprocity)의 원리와 보상(補賞, complement)의 원리를 제 1의 구성원리로 하며 공간에서 서로에 대한 역과 공통성(common property)을 갖고 자기유사를 지닐 때 연속체(continuum)를 손상하지 않고 전체공간을 유기체적으로 분절한다.같은 pattern 이었다. 그러나 JR89주에서는 280kb 가 나타나지 않아 다른 분리균주와 구분되었다.과 밀가루국(麴) 사이에 차(差)가 별(別)로 없었다.果)에서 총지질(總脂質)을 구성(構咸)하는 지방산(脂肪酸) 조성(組成)은 $C_{18:2}$산(酸), $C_{16:0}$산(酸)의 순(順)으로 그 함량(含最)이 맞은데 비(比)하여 각획분(各劃分)의 지질(脂質)을 구성(構成)하는 지방산(脂肪酸) 조성(組成)은 $C_{16:0}$산(酸), $C_{18:2}$산(酸)의 순(順)으로 그 함량(含量)이 많은 것으로 나타났으며 동결건조후(凍結乾燥後) 저장(貯藏)하는 동안에$C_{18:2}$산(酸), $C_{18:3}$산(酸)의 함량(含量)이 계속(繼續) 감소(減少)하고 있었다. 5. 4-monomethylsterol fraction에는 cycloartenol(20.6%)이 비교적(比較的) 높은 함량(含量)으로 함유(含有)되어 있었고, 그 외(外) cyclolaudenol, cycloeucalenol 및 citrostadienol 등이 함유(含有)되어 있었다. 6. 4-desmethylsterol fraction에