Kim, Jeong-Hee;Shin, Il Sheob;Cho, Kang-Hee;Kim, Se Hee;Kim, Dae-Hyun;Hwang, Jeong Hwan
Korean Journal of Breeding Science
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v.42
no.5
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pp.534-539
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2010
'Hongro' is early-mid maturing cultivar with good quality like 'Tsugaru' and it has not preharvest drop. The CAX1 gene was introduced into Korean apple cultivar 'Hongro' by Agrobacterium tumefaciens LBA4404 harboring pBI121 to obtain transgenic apple with enhanced Ca level. The CAX1 gene playing the role of $H^+/Ca^{2+}$ transporter from Arabidopsis thaliana increases Ca concentration in several plants. Regenerated transgenic lines were confirmed by polymerase chain reaction (PCR) analysis and Southern blot analysis of genomic DNA for the existence of CAX1 gene. Southern blot analysis of 'Hongro' transformants showed that two putative transgenic lines were integrated with CAX1 gene in genomic DNA. The CAX1 comparative expression levels of two transgenic lines were higher than that of non-transformant evaluated by comparative quantification analysis using a real-time PCR. These two lines were multiplied in vitro, and micro-grafted on apple rootstocks 'M.9' in the isolated greenhouse. Since two years after micro-grafting, the fruits came into bearing. Compared to Ca level of the non-transgenic 'Hongro', that of the CAX1 transgenic 'Hongro' in the flesh and leaves was higher.
Jung Gun Cho;Sung Ku Kang;Seung Heui Kim;Sang Kun Park;Yong Bum Kwack
Journal of Practical Agriculture & Fisheries Research
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v.25
no.4
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pp.84-89
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2024
Light is an important component among which plays a crucial role in determining the production and quality of fruit trees. Since the disturbance of light directly leads to reduced photosynthetic efficiency, their damage can be increased especially in fruit trees such as Japanese apricots with a short growing time. In this study, we investigated how the effects of shading condition can affect the production and quality of Japanese apricots according to increased damages by light disturbance in the main orchard complex. The average photosynthetically active radiation (PAR) level in Japanese apricots was rapidly dropped as the shading time was increased compared to the control (304 μmol/m2/s) and the PAR level decreased to 142 μmol/m2/s after shaded for eight hours. The maximum photosynthetic efficiency, with a PAR value of 900 to 1,000 μmol/m2/s, corresponds to the time period without shading and the time period with 2 hours of shading, and these times range from 11 a.m. to 3 p.m. And the time period for shading for 4 hours was from 1:00 p.m. to 2:00 p.m., and under conditions of shading for 6 and 8 hours, the effect was a low amount of light. There was no difference in the weight of Japanese apricots during 2 hours shading time, however, it was significantly reduced as shading time were increased. The difference of the acid content and L/D ratio was not significant on shading time, but the SSC was decreased as times going on. In conclusion, our results indicate that the shading for more than 2 hours make negative effects to decrease the weight and SSC and the yield and affects directly to drop in fruit quality.
Effect of gibberllin on the breaking of bud dormancy of root and growth of aerial parts were investigated under laboratory and field condition for the prolongation of shoot growth duration, shortening of fruit bearing age and the increase of root yield. Drop application of GA (0.5ml of 50ppm) on rhizome of one year old root broke bud dormancy better than by low temperature. Soaking for one hour of one year old roots which wintered in the field in GA (50-200ppm) greatly accelerated the emergence of new buds while kinetin was only effective at low level (50ppm). GA substantially increased stem length in early stage and petiole length later on while kinetin increased stem diameter. Under the field condition with polythylene film tunnel (PET) in early spring the soaking in GA (50ppm for 1 hour) of rhizome of 4 year old root with replanting and dropping GA (50ppm, 1ml) on rhizome without replanting brought earlier emergence (29days) in comparison with that in the usual field. PET alone caused 14 day-early emergence. GA increased the length of stem and petiole only in early stage and replanting decreased only petiole length in later stage. Soaking in GA with replanting caused the Pronounced decrease in peduncle length, percentage of (ruin set and dry weight of reproductive organ (fruits and peduncle). Dropping without replanting showed significant decrease only in dry weight of reproductive organ. Fruit maturing was 20 days earlier than in usual held with little difference between GA and PET. It is well expected that GA could be used for early emergence of bud, shortening of root dormancy period, thinning of fruit and higher root yield according to application amount and methods.
Horticulture, Environment, and Biotechnology : HEB
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v.59
no.6
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pp.815-826
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2018
The intense drought affecting olive production in Northern Chile underscores the need to research non-traditional irrigation strategies to obtain the best crop performance. Accordingly, this study aimed to obtain preliminary data to guide future research on this topic. Different water replenishment levels on crop evapotranspiration ($ET_c$ ; 13.5, 27.0, 40.5, and 54%) were established in a young orchard, cv. Arbequina, from the end of fruit drop (EFD) to full bloom in the next season. We evaluated the influence of plant water status (${\Psi}_{stem}$ ) and crop load, considered as function of fruit number divided by trunk cross-sectional area, on reproductive and productive variables using multiple linear regressions. Our results show that crop load and ${\Psi}_{stem}$ measured from EFD to harvest affected yield components. Nevertheless, ${\Psi}_{stem}$ had the strongest influence on fruit size, pulp development, oil accumulation, and yield. Oil content and yield were reduced by 54% and 50% for each MPa, respectively, from ${\Psi}_{stem\;EFD-H}$ -1.8 MPa, an effect that intensified as crop load increased. During the period of flower development (September-November), the number of flowers per inflorescence and percentage of perfect flowers were reduced when ${\Psi}_{stem}$ was less than -2.0 MPa. These preliminary results showed that bud differentiation, inflorescence and flower formation are highly sensitive to water deficit.
The aim of this study was to investigate the quality characteristics of Karedduk, Korean rice cakes, containing cactus (as Opuntia humifusa) fruit powder at a concentration of 0, 2, 4, 6 and 8% during three days of storage. The moisture content of the cooked cake just before storage ranged from 45.55~49.70%, indicating that the moisture content decreased as the amount of the powder added inceased. The L value, which is a measure of the lightness of the cake color decreased as the amount of added powder increased. In addition, the L value sharply decreased after one day of storage relative to its valve just after the cake was cooked. However, no significant changes were observed at longer storage times except at a fruit powder concentration of 2%. The a value, which is a measure of the redness, was significantly increased with an increase in the amount of added powder. The b value, which is a measure of the yellowness, was the same for all the treatments regardless of the amount of the powder added. The a and b values did not display any regular patterns in terms of the amount of powder added as a function of storage time. In regards to mechanical quality characteristics, the hardness and the gumminess of the cake just cooked tended to be increase with an increase in the amount of added powder indicating that no significant differences between the non-treatments and the treatments existed. The hardness of the cake did not change after two days of storage relative to when they were just cooked at all powder concentrations: however, the hardness of the cake after three days of storage was found to drop significantly with an increase in the amount of added powder. The cohesiveness of the cake right after cooking tended to decrease with an increase in the amount of added powder. The springiness of the cake right after it was cooked and on the second day of storage, did not significantly different as a function of powder concentration. The chewiness of the cake right after cooking increased with an increase in the amount of added powder. In the sensory test, the color and the flavor of the cake containing a powder concentration of 6% was found to be the highest. In addition and the chewiness significantly with an increase in the amount of added powder. In both taste and overall acceptability, the cake containing a powder concentration of 4% was determined to be the highest, showing no significant differences in taste relative to the cakes made with a powder concluded that it would be the best to cook Karedduk which a of cactus (Opuntia humifusa) fruit powder concentration of 4~6%.
Fruit drop due to Eurytoma maslovskii infestations of Japanese apricot (Prunus mume) is a serious economic issue in most parts of Jeonnam Province, with the exception of the coastal areas such as Wando, Shinan, Yeosu, and Muan. The average incidence of fruit drop was 67% in 2013 and 33.3% in 2014. E. maslovskii larvae overwinter inside the pits of Japanese apricots. Larval survival rates decrease to less than 30% after a rainy summer season. The eggs are long oval of 0.68 mm, 0.29 mm and ivory white. Mature larva is of 6.56 mm, 3.18 mm. Free pupa are black, adult females and males of 6.97 mm, 4.90 mm lengths, respectively, while the ovipositor is 0.64 mm in length. Adult emergence occurs from early April to early May, when Japanese apricot fruits are from 4 mm to 17 mm in diameter. Adults persist for 13.5 d after emergence, and the sex ratio of females to males was found th be 45.9:54.1. Although up to four eggs may be oviposited per fruit, only one larva will ultimately survive, ans the larvae are cannibalistic. The period during which E. maslovskii is able to oviposit on Japanese apricots only lasts from mid- to late April (fruit diameter: 12~16 mm). When the diameter of the fruit is >16 mm, the pit of the Japanese apricot hardens and larvae have difficultly penetrating the stone. Therefore, the most effective method of controlling this pest is to spray orchards with a control agent 2~3 times, at 5 d intervals, beginning in mid- April.
In order to evaluate the modified installation methods of roof ventilation devices, derived from the previous experiment ('investigation into the optimum capacity of roof ventilation devices and their deployment'), the conventional and modified (improved) roof ventilation systems were installed in the single-span plastic greenhouse for growing oriental melons. The roof vents ($60{\varphi}$) and roof fans (maximum air capacity of $38m^3/min$) were installed in the spacing of 15m (FT, modified 'side vent+roof fan' ventilation) and 6m (TT, modified 'side vent+roof vent' ventilation) respectively on the roof of greenhouses for the modified roof ventilation treatments, and 20m (FC, conventional 'side vent+roof fan' ventilation) and 8m (TC, conventional 'side vent+roof vent' ventilation) for the conventional ones. The stem diameter, leaf blade lengh, petiole length, and leaf width were lower in the FT and TT treatments than those in the conventional treatments, FC and TC. Although the fruit weight and total yields were slightly lower in the FT and TT treatments, the marketable fruit ratio (%) were higher, as a result of increased fruiting ratio (%) in these treatments, than those of FC and TC. The marketable yields (kg/10a) in the FT and TT treatments were 8,391 kg/10a and 7,283 kg/10a, which were respectively 661 kg/10a and 487 kg/10a higher than those in the treatments of FC and TC. The modified installation methods of roof fan resulted in production of more female flowers and lower fruit drop ratio (%) compared to conventional meathods. In the treatment of the conventional ventilation with roof vent, the fruit weight, fruit length & width, and flesh thickness were higher than in other treatments, but there were no significant differences in the fruit width and flesh thickness among the treatments.
This study aims to develop and apply a standard management self-diagnostic table for forestry households to better manage their astringent persimmon production. The diagnostic table consisted of 3 categories (general status of a forestry household, management performance indicators, level of management) and 18 subcategories. The current management status across 241 households was surveyed among 10 chief astringent persimmon producing municipalities. Scores from the 18 subcategories were aggregated for a total score to evaluate and compare different levels of management. The respondents scored an overall average of 57.4, 62% of which were placed between 40 and 60. Upon close examination Chungcheongnam-do scored a very low score in the 'management system-tree height' subcategory with respect to the average score potentially because of the large population of aged trees in the region. The national average in the 'production skills-time of fruit load adjustment' subcategory was only 1.96 because unlike sweet persimmons astringent persimmons adjust their own load by inducing physiological fruit drop. The national average in the 'management/sales skills-material purchase' subcategory was 2.01; over 60% of the respondents indicated a preference for independent selection and purchase for most materials. The households preferring a group purchase tended to utilize the regional co-op.
For this study, we designed and produced mulberry low-fat ice cream supplemented with synbiotics (MLF-ISS). The sensory characteristics and physical, chemical, and microbiological qualities of MLF-ISS were then determined. Mulberry juice inoculated with or without probiotic (Lactobacillus plantarum TISTR 926 and Saccharomyces boulardii CNCM I-745) was also tested at 37℃ for 24 h to determine probiotic growth rate, pH, total anthocyanin content (TAC), total phenolic content (TPC), and antioxidant activity (AA). Only the TAC of mulberry juice inoculated with S. boulardii CNCM I-745 increased considerably (p < 0.05) among these parameters. MLF-ISS was produced with varied mulberry fruit concentrations (0, 10, 20, 30, or 40%) (w/w). The MLF-ISS prepared with 30% mulberry fruit (w/w) (30-MLF-ISS) had a higher score in appearance, color, and sweetness (p < 0.05) when sensory qualities were measured using the 9-point hedonic scale method. In the CIE lab system (L*, a*, b*), the color values of 30-MLF-ISS were 27.80 ± 0.26, 12.99 ± 0.59, and 1.43 ± 0.05, respectively. The 30-MLF-ISS was also subjected to a proximate analysis. The melting rate of 30-MLF-ISS was 0.29 ± 0.03 g/min and the time it took for the first drop to fall was 37.00 ± 7.00 min. TAC, TPC, and AA of 30-MLF-ISS were observed to alter significantly (p < 0.05) during varied intervals of storage at - 18℃ (0, 30, and 60 days). The viability of probiotics in 30-MLF-ISS slightly decreased after storage at -18℃ for 8 weeks, but remained about 6 log CFU/g. During storage at -18℃ for 0 and 120 days, no pathogenic bacteria were detected in 30-MLF-ISS. These findings show that 30-MLF-ISS has nutritional and functional value, is free of foodborne pathogenic bacteria, is safe for consumers' health, and is suitable for application in the ice cream and related food industries.
Salinity problems are caused from the accumulation of soluble salts in the root zone. These excess salts reduce plant growth and vigor by altering water uptake and causing ion-specific toxicities or imbalances. In this investigation, green pepper cultivation technique using mixture bed of soil and rice hull and surface drop fertigation system was examined to prolong the productivity of salt-affected plastic film house soils. Green pepper growth was better in the mixture bed of soil and rice hull comparing to the conventional soil cultivation. Especially root growth was much better and the root had more thin root system in the mixture bed of soil and rice hull. The better growth of root may be due to the better physical conditions and lower EC in the mixture bed of soil and rice hull where nutrient supply was well-managed with fertigation system. In the cultivation with mixture bed of soil and rice hull, fruit yield of green pepper was significantly higher; increased by 43% in comparison to the conventional soil cultivation. Pepper cultivation technique using mixture bed of soil and rice hull and surface drop fertigation system is expected to be a useful method for maintaining and prolonging the productivity of salt-affected plastic film house soils.
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