• 한방안이비인후피부과학회지
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• 제35권4호
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• pp.75-94
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• 2022

Objectives : To identify the active ingredient of Poncirus Trifoliata Immaturus and to explore the mechanism expected to potentially act on dermatitis accompanied by pruritus. Methods : We conducted the network pharmacological analysis. We selected effective ingredients among the active compounds of Poinciri Fructus Immaturus. We found the target protein of the selected active ingredient, disease(dermatitis accompanied by pruritus) and fexofenadine. Then we established the network between the proteins which Poinciri Fructus Immaturus and fexofenadine intersected with disease respectively, and the coregene was also extracted. After that, the active pathways in the human body involving the groups and coregenes were searched. Results : Total of 7 active ingredients were selected, and 202 target proteins were collected. There were 756 proteins related to inflammatory skin disease accompanied by pruritus, and 75 proteins were related to fexofenadine. 42 proteins crossed by Poinciri Fructus Immaturus with a disease, and 31 proteins crossed by fexofenadine with a disease. 12 proteins were found as a coregene from the proteins that cross Poinciri Fructus Immaturus and disease. Coregenes are involved in 'Nitric-oxide synthase regulator activity', 'Epidermal growth factor receptor signaling pathway'. 2 groups that extracted are invloved in 'Fc receptor signaling pathway', 'Central carbon metabolism in cancer', 'Phosphatidylinositol 3-kinase complex, class IB', and 'omega-hydroxylase P450 pathway'. Conclusion : It is expected that Poinciri Fructus Immaturus will be able to show direct or indirect anti-pruritus and anti-inflammatory effects on skin inflammation accompanied by pruritus in the future. And it is also expected to have a synergy effect with fexofenadine on skin disease.

• 동의생리병리학회지
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• 제16권1호
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• pp.67-71
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• 2002

This study investigated the relaxation effects of Crataegi Fructus(CF, Crataegus pinnatifida Bunge) on the contraction evoked by phenylephrine in rabbit carotid artery, and also analyzes antioxidative status in vitro. CF revealed siginificant relaxation on phenylephrine-induced arterial contraction. It's relaxant effect statistically significant in both in the presence of endothelium and absence of endothelium, but statistically exerted more strong relaxation in the presence of endothelium. CF increased in vitro nitric oxide(NO) production in dose-dependent manner. Also, they reduced malondiaidehyde(MDA) concentrations, phosphatidyl choline-liposome(PCOOH) contents, linoleic acid-induced lipid peroxidation and exerted 1,1-diphenyl-2- picryl-hydrazyl(DPPH) radical scavenging effect, in vitro. These results indicate that Crataegi Fructus would be effective in relaxing arterial contraction and it's antioxidative effects may be involved in endothelium-dependent relaxation of artery via vascular protective properites.

• 동의생리병리학회지
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• 제16권5호
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• pp.1060-1065
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• 2002

LC20 phosphorylation and PKC α play an important role in modulation of contractile activity of smooth muscle. Besides, myosin phosphatase is also related with smooth muscle contraction in signaling pathways. We previously demonstrated that Crataegi Fructus inhibited phenylephrine-induced contraction and which might be implicated in nitrite formation(Son et al., 2002). In this study, we investigated the effects of butanol fraction of Crataegi Fructus(BFFC) on the localization of α-protein kinease C(PKC α) and myosin phosphatase subnits(MPs) in freshly isolated single ferret potal vein cells, and phosphorylation of LC20 during phenylephrine stimulation. In PKC α and MPs localization, BFFC blocked its translocation from the cytosol to the cell membrane by treatment of phenylephrine. BFFC have also dephosphorylated LC20 phosphorylation by phenylephrine stimulation under basal level, but no significant. These results indicate that the relaxation effect of BFFC is associated with inhibition of PKC α activation and MPs dissociation, and thus myosin phosphatase activity may be increased.

• 동의생리병리학회지
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• 제16권5호
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• pp.911-913
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• 2002

Rubi Fructus (fruit of Rubus coreanus Miq.), oriental medicine, has been used for remedy of the liver diseases and for tonic. In order to investigate the oxygen free radical, a harmful factor of aging, in liver of rats fed diets supplemented with the Rubi Fructus water extract(RX), Sprague-Dawley male rats have been fed a diet supplemented with 3% or 5% RX for a month. In rats fed 3% RX supplemented diet, hepatic cytochrome P-450 contents appeared to be increased, and catalase and superoxide dismutase activities were significantly increased compared with the control. There was no difference in glutathione peroxide and glutathione-S-transferase activities between the rats fed RX supplemented diets and the control diet. In conclusion, it is likely that rats fed a diet supplemented with RX may have the oxygen free radicals detoxication potential.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.195-200
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• 2003

The growth of S. aureus was inhibited by combination of natural products bearing antioxidative capacity and combined two, three and four kinds of them. S. aureus was controlled by Paeonia Japonica, Galla Rhois, Geranii Herba, alone, and two mixed combinations of Paeonia japonica and Scutellaria Baicalensis George, Paeonia japonica and Theae Folium, Paeonia japonica and Chelidonium Majus L, Paeonia japonica and Geranii Herba, Paeonia japonica and Schizandrae Fructus, and three mixed combinations of Paeonia Japonica and Scutellaria Baicalensis George and Geranii Herba, Paeonia Japonica and Scutellaria Baicalensis George and Chelidonium Majus L., Paeonia Japonica and Scutellaria Baicalensis George and Theae Folium, and four mixed combinations of Scutellaria Baicalensis George and Chelidonium Majus L. and Theae Folium and Schizandrae Fructus, Paeonia Japonica and Scutellaria Baicalensis George and Chelidonium Majus L. and Schizandrae Fructus, Scutellaria Baicalensis George and Geranii Herba and Theae Folium and Schizandrae Fructus. As these combinations of natural products will activate some parts of body, they may be applied to pharmaceutical applications, functional foods, antiaging tea, also expected to control bacterial growth for fermentative beverage bearing multifunction.

• 동의생리병리학회지
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• 제34권1호
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• pp.1-6
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• 2020

Arctii Lappa Fructus has the numerous health benefits, including antioxidant, anti-inflammatory, and anti-carcinogenic properties. Skin lipids are one of several factors that maintain epidermal barrier function. This study was to explore the lipogenic effect by ethanol extract of Arctii Lappa Fructus (EAF) in sebocytes. First, it was confirmed that EAF exhibited high antioxidant activity and collagenase activity inhibition. We found that cholesterol and triglyceride levels of cells by EAF were increased significantly in a dose-dependent manner. Moreover, EAF increased the expression of transcription factor sterol regulatory element-binding protein-1 (SREBP-1) in the cells. These results suggest that EAF induces lipogenesis in cells through the activation of SREBP-1.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.202-202
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• 1996

The antihistaminic action of eighteen herbal medicines was investigated by the radioligand binding and functional assays. The hexane fractions of Trichosanthis radix, Mori cortex radicis and Evodiae fructus dose-dependently inhibited [$^3$H]mepyramine binding to H$_1$ receptor and histamine-induced contraction in guinea-pig brain homogenates and isolated guinea-pig ilea, respectively. Antihistaminic action of the hexane and ethylacetate fractions of Mori cortex radicis and the hexane fraction of Evodiae fructus was more potent than their antimuscarinic action evaluated from the inhibition of [$^3$H]QNB binding and carbachol response. The ethylacetate and chloroform fractions and six known flavonoids from Scutellariae radix also inhibited histamine-induced contraction, but antihistaminic potencies of these fractions and compounds were almost identical with their antimuscarinic potencies. The hexane fractions of Mori cortex radicis and Evodiae fructus, as shown in ketotifen, inhibited selectively the increase of cutaneous vascular permeability induced by histamine. However, wogonin (SC-1) from Scutellariae radix was a nonselective inhibitor for the effect of histamine and serotonin on the vascular permeability. These results demonstrate that the hexane and ethylacetate fractions of Mori cortex radicis and the hexane fraction of Evodiae fructus have the selective histamine H$_1$ receptor blocking activities.

• 대한한의학방제학회지
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• 제22권1호
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• pp.1-11
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• 2014

Objectives : The purpose of this study is to find out herb-pair prototype composed of Pericarpium Zanthoxyli in "Donguibogam", to investigate origin of them. Methods : We researched the usages of Pericarpium Zanthoxyli compatibility as following procedure : 1. Choosing a related words such as Pericarpium Zanthoxyli(蜀椒, 川椒, 花椒) 2. Searching prescriptions: We searched prescriptions containing the related words in "Dongeuibogam" through "wook system", analyzed the selected prescription and found out herb-pair prototype composed of Pericarpium Zanthoxyli. Results : We found 12 fomulas in which Pericarpium Zanthoxyli herb pair was combinated and We made a Table 1. for the description of name of fomula, indication and herb configuration. Conclusions : Pericarpium Zanthoxyli was paired with below herbs; 檳榔(Arecae Semen), 細辛(Asari Radix), 苦楝根(Meliae Radix), 蔥白(Allii Fistulosi Bulbus), 茴香(Foeniculi Fructus), 巴豆(Crotonis Semen), 蓽撥(Piperis Longi Fructus), 露蜂房(Vespae Nidus), 肉桂(Cinnamomi Cortex), 艾葉(Artemisiae Argyi Folium), 細茶(Camellia sinensis), 雄黃(Realgar), 杏仁(Armeniacae Amarum Semen).

• 동의생리병리학회지
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• 제18권2호
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• pp.484-489
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• 2004

Objective : In order to study the effects of Herba ChelidoniiㆍClematis Florida ThunbㆍChaenomelis Fructus complex herbal acupuncture on anti-inflammation and liver in rats with arthritis induced Freund's complete adjuvant. Method : We performed several experimental items : that is edema value, numbers of WBC. total protein, total bilirrubin, SGOT, SGPT. Result: The change in the right plantar edema shows that Control group was reduced by 0% but, ST group ,Sample A group and Sample B group were reduced by 287.2%, 343.0%, 272.5% respectively. In the WBC count, ST group, Sample A group, Sample B group showed a decrease with statistical significance as compared with control group. In the total protein, Sample B group showed a decrease with statistical significance as compared with control group. In the total bilirrubin, SGOT, SGPT, None showed noxious variation with statistical significance as compared with control group. Conclusion : As a result of this experiment, it is concluded that Herba ChelidoniiㆍClematis Florida ThunbㆍChaenomelis Fructus complex herbal acupuncture showed that therapeutic effect of anti-inflammation in adjuvant arthritis and non-toxic effect of liver.

• 대한한의학방제학회지
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• 제18권1호
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• pp.155-167
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• 2010

Objectives : In order to investigate the antidepressant effects of Forsythiae Fructus(FF), we performed the Forced Swimming Test(FST). Also the expressions of corticotropin-releasing factor (CRF), c-Fos and tyrosine hydroxylase(TH) were measured by immunohistochemical method at paraventricular nucleus(PVN), ventral tegmental area(VTA) and locus coeruleus(LC). Methods : Spraque-Dawley rats were administered FF extract(100 mg/kg, 400 mg/kg) intragastrically three times prior to the FST. Results : The duration of immobility in the FST was significantly decreased in the FF 100 mg/kg, 400 mg/kg groups(p<0.05). The expression of CRF was significantly reduced in the FF 100 mg/kg and 400 mg/kg groups(p<0.001). c-Fos expression was significantly decreased at PVN in the FF 100 mg/kg group(p=0.069). TH expression at VTA was significantly increased in the FF 100 mg/kg and 400 mg/kg groups(p<0.05). TH expression at LC was not significantly changed(p=0.346). Conclusion : According to the results, it can be suggested that Forsythiae Fructus has antidepressant effect via the decreased immobility through the reduction of CRF and c-Fos expression at PVN.