• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.177-179
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• 2002

The process for the production of mannitol with fructose (5% to 25%) using Leuconostoc mesenteroides NRRL B-1149 was investigated. Optimization study for mannitol production was carried out in 8 liter batch or fed-batch cultures at $28^{\circ}C$, pH 5.0, without aeration. When 5% fructose was used in a batch culture fermentation, the yield of mannitol was 78% of theoretical. As the concentration of fructose was increased to 10% in a batch culture, the yield was reduced to 59.6% of theoretical. Using a fed-batch fermentation with 10% fructose, the yield was increased to 81.9%. When 15% fructose was used for a fed batch fermentation 5% fructose was initially added and the last 10% fructose was supplied continuously. The final yield of mannitol was 83.71% of theoretical. When 20% fructose was used, the yield was more higher, 89.48%.

• Applied Biological Chemistry
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• v.27 no.3
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• pp.158-162
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• 1984

In order to estimate the basic data for the processing suitability of fruits, the contents of free sugars in citrus fruits(14 varieties) cultured in Jeju island, and peaches(4), grapes(4), pears(3) and apples(6) obtained from a local market were investigated by HPLC. The sucrose was the most abundant sugar in citrus fruits and peaches, and followed by fructose and glucose in order. The sugar contents in citru fruits was a wide difference according to the variety. In citrus fruit, the sucrose content was about 53 to 65% to total free sugars and the ratio of fructose to glucose was 1.0/0.8-1.1. The sugars present in grapes and pears were fructose of most abundance, glucose and sucrose in order. Jangshiprang pear showed 2 times higher fructose content than other variety. The free sugar in Taeyang and Hongok(Jonathan) apples were fructose, glucose and sucrose in order of abundance which fructose, sucrose and glucose in Golden Fuji and Mutsu.

• Korean Journal of Pharmacognosy
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• v.33 no.4 s.131
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• pp.384-388
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• 2002

The effects of the ethanol (EtOH)-extract of Allium wageki were studied on the changes of body weight (BW), systolic blood pressure (SBP), triacylglycerol (TG), cholesterol, and insulin levels in Sprague-Dawley rats in which high fructose feeding elicited hyperinsulinemia, hypertriglyceridemia, and hypertension. SBP and BW were measured for 7 weeks on the fructose diet, fructose/EtOH-extract, vehicle- treated, or vehicle/EtOH-treated rats. The EtOH-extract of Allium wageki blocked the increases of SBP ($119.4{\pm}3.7\;mmHg$ in fructose/EtOH-extract of Allium wageki; $141{\pm}4.3 \;mmHg$ in fructose diet, p<0.01) and BW ($368{\pm}11.4\;g$ in fructose/EtOH-extract of Allium wageki; $402{\pm}15.7\;g$ in fructose diet, p<0.05) by high fructose-diet. The increases of TG, total cholesterol, and insulin levels were also blocked by administration of EtOH-extract of Allium wageki in fructose-induced hypertensive rats. These results suggest that EtOH-extract of Allium wageki has protect effects on the increases of TG, total cholesterol, insulin level, and blood pressure in fructose-induced hypertensive rats.

• Journal of Microbiology and Biotechnology
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• v.26 no.9
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• pp.1579-1585
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• 2016

Sugar esters are valuable compounds composed of various sugars and fatty acids that can be used as antibacterial agents and emulsifiers in toothpaste and canned foods. For example, fructose fatty acid esters suppress growth of Streptococcus mutans, a typical pathogenic bacterium causing dental caries. In this study, fructose laurate ester was chosen as a target material and was synthesized by a transesterification reaction using Candida antarctica lipase B. We performed a solvent screening experiment and found that a t-butanol/dimethyl sulfoxide mixture was the best solvent to dissolve fructose and methyl laurate. Fructose laurate was synthesized by transesterification of fructose (100 mM) with methyl laurate (30 mM) in t-butanol containing 20% dimethyl sulfoxide. The conversion yield was about 90%, which was calculated based on the quantity of methyl laurate using high-performance liquid chromatography. Fructose monolaurate (M_r 361) was detected in the reaction mixture by high-resolution mass spectrometry. The inhibitory effect of fructose laurate on growth of oral or food spoilage microorganisms, including S. mutans, Bacillus coagulans, and Geobacillus stearothermophilus, was evaluated.

• The Korean Journal of Food And Nutrition
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• v.29 no.6
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• pp.889-898
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• 2016

This study was to evaluate the characteristics of bread and the rheology of flour dough containing sugar alcohols, after addition of fructose. In the farinogram tests, the addition of sugar alcohol changed the stability and mixing tolerance index. The stability and mixing tolerance index of farinogram increased as the amount of sugar alcohols increased. Amylograms revealed that the increase in gelatinization temperature and maximum viscosity of wheat flour dough with sugar alcohols was more than that of controls. Extensogram of dough with sugar alcohols exhibited higher extensibility and resistance. After fermentation treatment, the dough volumes prepared with only sorbitol and xylitol were lesser than those prepared after addition of fructose. The volume of loaf and specific volume of bread containing sugar alcohols with fructose significantly increased. The breads containing sugar alcohols showed greater taste, flavor and texture scores, for breads prepared with either sorbitol with fructose or xylitol with fructose, compared to breads without fructose. Overall preference scores by sensory evaluation of bread containing sugar alcohols with fructose were higher than bread with only sugar alcohols. These results indicate that the addition of fructose improves the flavor of bread containing sugar alcohols.

• Microbiology and Biotechnology Letters
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• v.9 no.1
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• pp.45-50
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• 1981

The effect of growth and the carbon sources including the molar ratio of fructose to glucose was studied for the maximization of inosine-5'-monophosphate (5'-IMP) production from Brebibacterium ammoniagenes D-21530. According to experimental results, fructose was more efficient to 5'-IMP accumulation than glucose, while the latter was better for the cell growth than the former. To synchronously use glucose and fructose as carbon source is to optimally control the cell growth and maximum production of 5'-IMP without change of other conditions. The optimal weight percent of fructose to sum of glucose and fructose was 20～40%, and the productivity improvement over the utilization of fructose was about 40%. And also the optimality of purine base such as adenine and guanine were considered. The optimal concentrations of adenine and guanine were near 50㎎/l.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.141.1-141
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• 2003

향미가 우수한 음나무 차를 제조하기 위한 최적의 기질물질 선별 실험으로 당, 아미노산 첨가하여 관능검사를 실시하였다. 여러 종류의 당(fructose)과 아미노산(threonine)을 첨가 및 혼합하여 그 혼합용액에 3시간 침지시킨 후 12시간 동안 건조하여 10$0^{\circ}C$, 4분 동안 볶음처리하여 중심합성계획에 의해 관능검사를 실시하였다. 그 결과 색상에 관한 관능평가에서 fructose 12%, threonine 2% 첨가한 음나무 껍질이 가장 우수한 것으로 나타났으며, fructose 9%, threonine 1.5% 첨가한 음나무피가 향미에서 가장 높은 관능평가를 나타내었다. 맛에서는 fructose 6%, threonine 1% 첨가한 음나무피가 가장 높은 것으로 나타났다. 또한, 전반적 기호도에서는 fructose 6%, threonine 3%와 fructose 6%, threonine 1% 첨가가 우수한 것으로 나타났다. 관능적 품질 평가에서는 아미노산보다 당의 함량에 따라 영향을 많이 받는 것으로 나타났으며 당의 함량이 높을수록 관능적 품질평가도 높은 것으로 나타났다. 특히 맛에 대한 관능평가에서 당의 함량이 증가할수록 높았으며 전반적인 기호도에서는 당과 아미노산의 함량이 증가할수록 감소하다가 일정시점 이후로는 오히려 증가하였다.

• The Journal of Korean Academy of Prosthodontics
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• v.44 no.1
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• pp.124-134
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• 2006

Statement of problem: Streptococcus produces energy and forms extracellular polysaccharides by metabolizing sucrose. Insoluble glucan, a kind of extracellular polysaccharide, is the important material of dental plaque. Fructose affects the metabolism of sucrose. Purpose: The purpose of this study was to evaluate the effect of fructose on the metabolism of sucrose in Streptococcus mutans. Materials and methods: To determine the effect of fructose on the formation of artificial plaque by Streptococcus mutans Ingbritt, S. mutans and fructose were placed in beakers containing M17 broth and sucrose. The wires were hung on frameworks inserted into cork stoppers, and then immersed in each of the beakers. After the incubation with gentle shaking, each wire was weighed. To analyze the effect of fructose on the sucrose metabolism by S. mutans or glucosyltransferase, S. mutans and fructose were placed in M17 broth containing sucrose. After the incubation. the remaining sucrose and polymers were analysed by thin layer chromatography. Results: The following results were obtained; 1. When Streptococcus mutans was cultured in the media containing 3% sucrose for 8 hours, the mean weight of formed artificial plaque on the wires was $124.3{\pm}3.0mg$, whereas being reduced to $20.7{\pm}10.2mg$ in the media added with 3% sucrose and 4% fructose(p<0.05) 2. When the control containing glucose was added with sucrose, the optical density of Streptococcus mutans solution cultured for 24 hours was not increased compared with the control, while being increased by adding with fructose. 3. When Streptococcus mutans was incubated in the media added with sucrose and fructose for 8 hours, the number of viable cells was increased compared with the media added with sucrose. 4. The amount of remained sucrose was increased in Streptococcus mutans culture supernatant of media added with sucrose and fructose than with sucrose only. but the amount of produced insoluble glucan was decreased. 5. The amounts of remained sucrose and produced soluble glucan were increased in the culture of glucosyltransferase-contained media added with sucrose and fructose than with sucrose only, but the amount of produced insoluble glucan was decreased . Conclusion: These results indicated that the sucrose metabolism and the production of insoluble glucan were inhibited in Streptococcus mutans by adding fructose in the media containing sucrose.

• Journal of Embryo Transfer
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• v.32 no.3
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• pp.139-146
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• 2017

The objective of this study was to determine the effect of fructose that was supplemented to a chemically defined in vitro maturation (IVM) medium on oocyte maturation and embryonic development after parthenogenesis in pigs. The base medium for in vitro maturation (IVM) was porcine zygote medium (PZM) that was supplemented with 0.05% (w/v) polyvinyl alcohol (PVA) or 10% (v/v) porcine follicular fluid (pFF). In the first experiment, when immature pig oocytes were matured in a chemically defined medium that was supplemented with 5.5 mM glucose or with 1.5, 3.0 and 5.5 mM fructose, 3.0 mM fructose resulted in a higher nuclear maturation (91.5%) than 1.5 and 5.5 mM fructose (81.9 and 81.9%, respectively) but showed a similar result with 5.5 mM glucose (94.2%). However, there was no significant differences among groups in the embryo cleavage (89.4-92.4%), blastocyst formation (37.5-41.1%), and mean cell number of blastocyst (30.8-34.2 cells). Fructose at the concentration of 3.0 mM (1.08 pixels/oocyte) resulted in a higher intra-oocyte glutathione (GSH) content than 1.5 and 5.5 mM fructose (1.00 and 0.87 pixels/oocytes, respectively) while the cumulus cell expansion was not influenced. In the second experiment, effect of individual and combined supplementation of a chemically defined maturation medium with 5.5 mM glucose or 3.0 mM fructose was examined. No significant effect was found in the nuclear maturation (86.3-92.6%). Embryo cleavage was significantly increased by the combined supplementation with glucose and fructose (95.2%) compared to that with 3.0 mM fructose only (85.7%) while blastocyst formation (37.3-42.8%) and embryonic cell number (33.3-34.1 cells) were not altered. Effect of supplementation of pFF-containing medium with glucose and fructose + glucose was examined in the third experiment. No significant effect by the supplementation with glucose and fructose or glucose alone was observed in the nuclear maturation of oocytes (90.7-94.1%) and blastocyst formation (51.0-56.5%). Our results demonstrate that 3.0 mM fructose was comparable to 5.5 mM glucose in supporting in vitro oocyte maturation and embryonic development after parthenogenesis and could be used as an alternative energy source to glucose for in vitro maturation of pig oocytes.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.6
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• pp.705-709
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• 1996

A simplified dilutor for cock spermatozoa at ambient temperature was achieved by adjusting the 5% concentration of fructose in isotonic saline. Motility of cock spermatozoa was arrested completely for maximum 6 hrs without affection the survivability of spermatozoa by employing this sugar. To study the effect of high concentration of fructose on fertility, sperm were inseminated with or without fructose at different hrs. Fructose from semen samples was removed by centrifugation. High fertility obtained in the hens inseminated with fructose free sperm (washed). In addition, washed sperm maintained the 85.00% fertility for 6 hrs in winter season ($17-21^{\circ}C$) and 82.67% fertility for 3 hrs in summer season ($31-35^{\circ}C$). Whereas control groups showed 47.33 and 25.33% fertility in winter and summer season respectively. No significant difference was found in percent motility and live counts between the control and washed experimental groups during winter season. However, these measures differed significantly in summer. Washing of cock spermatozoa more than once, high speed centrifugation and more duration for centrifugation proved harmful to fertility. It may be concluded that fructose (5%) can be used as a motility or metabolic inhibitor of spermatozoa for short-term storage of cock semen at ambient temperatures.