• 제목/요약/키워드: freshwater culture

검색결과 123건 처리시간 0.024초

Additions to the six taxa of the genus Cosmarium (Desmidiaceae, Charophyta) in Korea

  • Lee, Ok-Min
    • Journal of Ecology and Environment
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    • 제38권4호
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    • pp.629-636
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    • 2015
  • The samples were collected at lowland swamps, reservoirs, mountainous wetlands, and sphagnum bogs from 2012 to 2014. The followings were newly recorded in Korea: two species, three varieties, and one form, including six taxa of the genus Cosmarium. The newly recorded Korean species were Cosmarium bioculatum var. hians, C. bireme, C. pseudobiremum, C. nitidulum var. pseudorectangulare, C. trilobulatum f. retusum, and C. trilobulatum var. depressum. The flora of the genus Cosmarium contains 303 taxa in total in Korea. The specimens were cultured and deposited on the algal culture collection of Kyonggi University (ACKU) and National Institute of Botanical Resources (NIBR).

현장(現場)에 있어서 양식(養殖)넙치의 질병대책(疾病對策) (Control Methods of Diseased of Japanese Flounder, Paralichthys olivaceus, used in Fish Farms, in Japan)

  • 미즈노 요시츠구
    • 한국어병학회지
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    • 제6권2호
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    • pp.219-231
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    • 1993
  • The author introduces the preventive and therapeutic methods of diseases in Japanese flounder which have been conducted by the Fish Disease Laboratory, the Mikamewan Fishery Cooperative Union, Ehime Prefecture, since 1982. Prevention 1. Addition of a sand substrate in the culture pond was effective to prevent ulcer disease. 2. Bathing in 0.5ppm of copper ion was effective to prevent some parasites. 3. Low stocking density of the fish reduced an incidence of edwardsiellosis or gliding bacterial disease. 4. Removal of the diseased fish prevented thd spread of lyphoeystis. 5. So-called $\pi$-water was effective to prevent the fry from some diseases. 6. Immersion of the juvenile in a sodium nifrusylate solution during transportation was effective to prevent gliding bacterial disease. Therapy 1. Sodium nifrustylate or oxytetracycline was effective to cure gliding bacterial disease. 2. Bathing in formalin(150ppm) or freshwater was effective to cure scuticociliatidosis. 3. Erythromycin was effective to cure $\beta$-hemolytic Streptococcus sp. infection. 4. Bathing in a hydrogen peroxide solution(1.5%) was effective to cure white spot disease.

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광반응기와 Euglena gracilis Z를 이용한 이산화탄소 고정화 공정의 거동 특성 (The Behavior of a $CO_2$Fixation Process by Euglena Gracilis Z with a Photobioreactor)

  • 신항식;채소용;황응주;임재림;남세용
    • KSBB Journal
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    • 제15권6호
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    • pp.644-648
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    • 2000
  • Biological fixation of carbon dioxide using microalgae is known as an effective CO$_2$reduction technology. However, many environmental factors influence microalgal productivity. Optimal cultivation factors were determined for the green alga, Euglena gracilis Z, which offers high protein and vitamin E content for animal fodder. In batch culture in a photovioreactor, it was found that theinitial pH, temperature, CO$_2$concentration in air, and light intensity during the optimal cultivating conditions were 3.5, 27$^{\circ}C$, 5-10% and 520 ${\mu}$mol/㎡/s, respectively. When tap water and freshwater were used as cultivating media unsterilized tap water was found to be effective. A kinetic model was considered to determine the relationship between the specific growth rate and the light intensity. The half-velocity coefficient (K(sub)I) in the Monod model under photoautotrophic conditions was 978.9 ${\mu}$mol/㎡/s.

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A Study of Seven Unrecorded Species of Benthic and Subaerophytic Cyanobacteria (Cyanophyceae, Cyanophyta) in Korea

  • Yim, Byoung Cheol;Kim, Jee Hwan;Yoon, Sung Ro;Lee, Nam Ju;Lee, Ok Min
    • 환경생물
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    • 제36권3호
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    • pp.291-298
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    • 2018
  • Samples were collected from benthic and subaerophytic habitats of fresh and brackish water in Korea from April 2017 to April 2018. Accordingly, three genera and seven species were newly recorded in Korea. Three unrecorded genera were Cephalothrix, Cyanophanon and Toxifilum, and seven species were Anagnostidinema pseudacutissimum, Calothrix elenkinii, Cephalothrix komarekiana, Cyanophanon mirabile, Cyanosarcina chroococcoides, Leptolyngbya ectocarpi and Toxifilum mysidocida. Anagnostidinema pseudacutissimum and Cephalothrix komarekiana had been previously reported to inhabit freshwater, but these were found to be present in brackish water during the period of the present study. Also, Cyanosarcina chroococcoides previously reported to inhabit moorland waters and swamps, were found to be present in subaerophytic habitat.

Five Taxa of Newly Recorded Species of Scenedesmaceae (Sphaeropleales, Chlorophyceae, Chlorophyta) in Korea

  • Bang, Sung Do;Kim, Jee Hwan;Yim, Byeong Cheol;Lee, Ok Min
    • 환경생물
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    • 제36권3호
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    • pp.271-276
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    • 2018
  • Phytoplanktons were collected from various environments including small marshes, small ponds, reservoirs and brackish water from March 2017 to November 2017. In this study, five species of family Scenedesmaceae were newly recorded in Korea. The genus Desmodesmus was newly reported in Korea. The newly recorded species are Scenedesmus nanus, S. praetervisus, Desmodesmus costato-granulatus, D. lunatus and D. spinulatus. S. nanus and S. praetervisus are known to occur in freshwater, but they were found in the brackish water in this study.

Isolation and Characterization of Previously Undescribed Seventeen Fungal Species Belonging to the order Hypocreales in Korea

  • Jaeduk Goh;Yoosun Oh;Young-Hwan Park;Hye Yeon Mun;Sangkyu Park;Wonsu Cheon
    • 한국균학회지
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    • 제50권1호
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    • pp.1-29
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    • 2022
  • Hypocreales is one of the largest orders within the class Sordariomycetes in Ascomycota. Several species of this order are cosmopolitan and have a broad range of habitats. Here, we isolated several fungal strains from environmental samples, including freshwater sediment and plant litter. The strains were identified via molecular and phylogenetic analyses of rDNA and other DNA markers, such as TUB, RPB2, and EF1. The morphological characteristics of the fungi were investigated using microscopy, and culture characteristics were assessed from their growth on several media. We identified 17 species previously unrecorded in Korea: Dactylonectria hordeicola, Flavocillium bifurcatum, Fusarium luffae, Ilyonectria ilicicola, Ilyonectria qitaiheensis, Ilyonectria robusta, Lecanicillium aphanocladii, Nectria ulmicola, Neonectria lugdunensis, Ovicillium oosporum, Pseudonectria foliicola, Sarocladium spinificis, Scolecofusarium ciliatum, Trichoderma appalachiense, Trichoderma subviride, Trichoderma taiwanense, and Trichoderma tsugarense.

실내사육조건에서 한국산 물벼룩 종간 life cycle 비교 (Comparison of Life Cycle of Several Korean Native Freshwater Cladocerans in Laboratory Culture Conditions)

  • 김병석;박연기;박경훈;신진섭;김진화;윤성명;안용준
    • 농약과학회지
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    • 제10권3호
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    • pp.196-200
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    • 2006
  • 국내서식 물벼룩종을 이용한 표준생태독성시험법 개발을 위한 기초연구로서 국내환경에서의 대표성, 실내사육가능성 및 독성시험에 적합한 크기 등을 고려하여 선발된 4종의 한국산 물벼룩 Daphnia sp., Daphnia obtusa, Moina macrocopa, S.imocephalus vetulus과 Daphnia magna에 대한 실내사육조건에서의 life cycle을 조사하였다. 국내산 물벼룩 4종과 D. magna에 대한 실내사육조건에서의 전 생애에 걸친 생육특성을 조사한 결과 D. magna가 어미 1마리당 평균 358마리의 새끼를 출산하였고 국내산 물벼룩 중에는 Daphnia sp.가 297마리의 새끼를 출산하여 가장 출산새끼수가 많았으며 그 다음으로는 M. macrocopa, S. vetulus, D. obtusa 순이었다. 평균수명은 D. magna가 50일로 가장 길었으며 국내산 물벼룩 중에는 Daphnia sp.가 39일로 조사되었으며 그 다음으로는 S. vetulus, D. obtusa M. macrocopa, 순이었다. 특히 M. macrocopa의 경우 번식력이 우수하면서도 세대기간이 짧아 기존의 번식독성시험법에서 제시하는 21일보다 짧은 10일 정도의 단기간에 번식영향을 평가할 수 있을 것으로 판단된다.

Burkholderia sp. OS17의 항균활성 증진을 위한 배양최적화 (Antimicrobial activities of Burkholderia sp. strains and optimization of culture conditions)

  • 남영호;최아영;황병수;정유진
    • 미생물학회지
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    • 제54권4호
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    • pp.428-435
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    • 2018
  • 본 연구는 담수환경에서 항균활성을 보유한 미생물을 발굴하고, 활성 증진을 위해 배양조건을 최적화하는 것이다. 상주시 중동면 오상저수지에서 시료를 채취하여 38종의 미생물을 순수분리하였다. 16S rRNA 염기서열 분석에 근거하여 Proteobacteria강(22종), Actinobacteria강(7종), Bacteroidetes강(6종), Firmicutes강(3종)으로 구성되어있는 것을 확인하였다. 메티실린내성 황색포도상구군 등 10종의 유해미생물에 대한 항균활성을 보유한 Burkholderia sp. OS17 균주를 선발하였다. 항균활성 증진을 위한 상용배지, 온도, 초기 pH별 생육 및 항균활성 비교실험을 수행하였다. OS17 균주는 YPD 배지, $35^{\circ}C$, pH 6.5로 배양했을 때 가장 활성이 높았다. LB, NB, TSB, R2A 배지와 $20^{\circ}C$, $25^{\circ}C$ 배양했을 때는 생장은 가능하나 항균활성이 전혀 없었다. 이전결과를 바탕으로 YPD 배지, $35^{\circ}C$에서 배양하면서 5 L fermenter를 이용하여 생육, 항균활성, pH 확인을 통해 배양 48시간을 최적 배양시간으로 선정하였다. 항균활성을 보유한 미생물의 배양 최적화는 항균물질 생산에 영향을 미치고, 이는 상업적 응용에 이점으로 작용할 수 있다.

Magnesium Uptake by the Green Microalga Chlorella vulgaris in Batch Cultures

  • Ayed, Hela Ben Amor-Ben;Taidi, Behnam;Ayadi, Habib;Pareau, Dominique;Stambouli, Moncef
    • Journal of Microbiology and Biotechnology
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    • 제26권3호
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    • pp.503-510
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    • 2016
  • The accumulation (internal and superficial distribution) of magnesium ions (Mg2+) by the green freshwater microalga Chlorella vulgaris (C. vulgaris) was investigated under autotrophic culture in a stirred photobioreactor. The concentrations of the three forms of Mg2+ (dissolved, extracellular, and intracellular) were determined with atomic absorption spectroscopy during the course of C. vulgaris growth. The proportions of adsorbed (extracellular) and absorbed (intracellular) Mg2+ were quantified. The concentration of the most important pigment in algal cells, chlorophyll a, increased over time in proportion to the increase in the biomass concentration, indicating a constant chlorophyll/biomass ratio during the linear growth phase. The mean-average rate of Mg2+ uptake by C. vulgaris grown in a culture medium starting with 16 mg/l of Mg2+ concentration was measured. A clear relationship between the biomass concentration and the proportion of the Mg2+ removal from the medium was observed. Of the total Mg2+ present in the culture medium, 18% was adsorbed on the cell wall and 51% was absorbed by the biomass by the end of the experiment (765 h). Overall, 69% of the initial Mg2+ were found to be removed from the medium. This study supported the kinetic model based on a reversible first-order reaction for Mg2+ bioaccumulation in C. vulgaris, which was consistent with the experimental data.

강변공간의 문화축제 조성을 위한 융복합 기술의 활용 (Application on the Convergence Technology for Culture Festival Promotion of Riverside Space)

  • 연상호;이영욱
    • 문화기술의 융합
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    • 제1권4호
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    • pp.79-86
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    • 2015
  • 청풍호와 강변은 은 한국 반도의 중앙에 제천시에 위치하고 있으며, 1984년에 충주 수력발전댐 건설에 의해 형성된 청풍호수는 지금은 지난 30 년 동안 남한강 상류의 중심하천으로 만들어진 담수호처럼 변화되어져 있다. 최근 제천시 정부는 미래를 위한 자연치유 리조트와 엔터테인먼트 콘텐츠를 개발하고 있으며, 본 연구는 이 지역의 문화축제의 타당성 조사에 대한 비교에서 원격탐사 기술과 현장조사 계획을 사용하여 지리 조사 및 분석의 진행하였고, 매우 효과적인 것으로 입증 할 수 있는 항공사진과 원격탐사기법으로 자연환경과 지형분석을 실시하여 문화콘텐츠와 통합하고, 강변 공간에서 미래의 비즈니스 창출 및 관광자원의 조사를 통하여 신 브랜드의 시설과 이벤트를 구상하였다. 결과적으로, 보다 다양한 문화축제가 형성될 수 있도록 기존의 문화적인 콘텐츠에 새로운 첨단 디지털 영상기술을 융합실험으로 미래의 문화참여 관광의 새로운 방향을 제시하고자 하였다.