• Journal of the Korean Wood Science and Technology
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• v.49 no.4
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• pp.384-393
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• 2021

Recently, due to environmental and toxicity issues, there has been increasing attention on research regarding natural products that can reduce the use of chemical fertilizers. Wood extracts derived from the biorefining process contain various fertilizer ingredients. HPLC analysis revealed that wood extract contains approximately 5.2% hemicellulosic sugar. The growth of lettuce (Lactuca sativa) upon treatment with wood extract (extract obtained from steam-exploded pine) or water-soluble fertilizers containing different nutrients was analyzed in this study. After two weeks, the growth characteristics of lettuce as affected by wood extract or water-soluble fertilizers were significantly different. The effect of water-soluble fertilizers containing ascorbic acid, magnesium sulfate, citric acid, potassium nitrate, amino acids, or seaweed extract was less desirable than that of wood extracts regarding plant height (18.6 cm), number of leaves (10), leaf length (14.1 cm), shoot fresh wight (9.8 g/plant), root fresh weight (0.8 g/plant) and shoot dry weight (0.6 g/plant). The plant height, number of leaves, leaf length, shoot fresh wight, root fresh weight, shoot dry weight of water-soluble fertilizers containing wood extract were significantly different compared to the control (plant height :13.5 cm, number of leaves : 7, leaf length : 9.4 cm, shoot fresh wight : 5.3 g/plant, root fresh weight : 0.7 g/plant, shoot dry weight : 0.4 g/plant, root dry weight : 0.07 g/plant). From these results, it was concluded that wood extract can be used as a potential water-soluble fertilizer to increase the yield of leafy vegetables.

• Journal of agriculture & life science
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• v.46 no.4
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• pp.129-139
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• 2012

This study was carried out to evaluate the chemical properties and functional characteristics, such as general composition and bioactivity compounds contents of fresh and blanched (at $95^{\circ}C$, for 5min) garlic shoot from Namhae. Also, evaluated antioxidant and antimicrobial activities of water and ethanol extract of fresh and blanched garlic shoot. The moisture content of fresh garlic steam was $ 85.14{\pm}0.35%$, crude protein and crude lipid were $0.79{\pm}0.26%$ and $2.96{\pm}0.03%$ respectively. Vitamin C content was higher in fresh garlic shoot ($7.07{\pm}0.84mg/100g$) than blanched. Total phenol and total chlorophyll contents were respectively $16.93{\pm}1.17mM/g$ and $6.70{\pm}0.46mg/g$ in fresh garlic shoot. Allicin content of blanched garlic shoot was $128.63{\pm}1.59mM/g$. This content was 1.82 times higher than the fresh garlic shoot. Total pyruvate content was higher in fresh garlic shoot ($24.63{\pm}1.59mM/g$), but thiosulfinate was higher in fresh garlic shoot. Total flavonoide was the highest in water extract of blanched garlic shoot ($3.67{\pm}0.00mM/g$). ABTS radical scavenging activity of water extract form blanched garlic shoot was $85.09{\pm}0.28%$, which was higher than the other extracts. NO radical scavenging activity of ethanol extract from blanched garlic shoot was significantly higher than the extracts from fresh garlic shoot. Antibacterial activity to S. aureus, S. enterica, B. cereus and E. coli was only indicated in water extract of fresh garlic shoot.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.505-510
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• 2010

This study was conducted to refine a micropropagation method of water hyacinth (Eichhornia crassipes) in vitro. When young shoots were cultured on media with various concentrations of BA or TDZ alone, LS medium containing $5.0\;mgl^{-1}$ BA was found favorable for shoot proliferation from young shoots with a mean of 4.2 shoots. Using BA together with IAA, more shoots were obtained on LS medium containing $5.0\;mgl^{-1}$ BA and $1.0\;mgl^{-1}$ IAA with a mean of 5.7 shoots. In liquid medium, number of shoots and fresh weight per explant increased significantly. The best shoot proliferation and increasing of fresh weight were achieved on LS liquid medium containing $5.0\;mgl^{-1}$ BA and $1.0\;mgl^{-1}$ IAA with 6.9 shoots and more than 4,000 mg fresh weight. Of the different concentrations of LS salt, double strength of LS medium provided the highest shoot proliferation with 7.3 shoots, and fresh weight with 5,539 mg per explant. Shoot proliferation on LS medium containing $50\;gl^{-1}$ sucrose had better results with 8.7 shoots and 5,979 mg per explant in fresh weight than other conditions. In conclusion, the optimal level for shoot proliferation and biomass increase of water hyacinth was attained with the application of the double strength of LS medium containing $5.0\;mgl^{-1}$ BA, $1.0\;mgl^{-1}$ IAA and $50\;gl^{-1}$ sucrose.

• Journal of Bio-Environment Control
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• v.16 no.4
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• pp.388-394
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• 2007

To product the edible fresh shoots of Sedum sarmentosum in summer season, 4 local strains were cultivated with cutting propagation under 0, 30, 50, 70, 90% shading. Effect of shading on shoot growth, leaf chroma value, SPAD value, and bitterness were investigated at 35 days after cutting. Plant height, the number of node and leaf stem diameter in $30{\sim}90%$ shading were increased than in the control, and Pohang local strain had little-overgrowth shoot, thicker stem, a few brenches per shoot, and larger leaf compared to the other local strains. Fresh and dry weight under shading were increased than in the control, dry weight of pohang local strain was highest in 50% shading. Hunter L and b values were decreased out of proportion to higher shading rate. SPAD value in $50{\sim}90%$ shading was significantly lower than in the control. The bitterness of fresh shoot was decreased out of proportion to higher shading rate. In Summer season, producing the fresh shoot of S. sarmentosum was excellent in 50% shading, and Pohang local strain among 4 local strains was more stable and good in yield and quality under shading.

• Korean Journal of Environmental Agriculture
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• v.29 no.4
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• pp.408-416
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• 2010

To evaluate the effectiveness of AMF on the growth of horticultural crops, we compared mycorrhizal and non-mycorrhizal plants, perilla (P. frutescens Britt.), that were inoculated with AMF propagules. In the early stages of growth of perilla, compared to the AMF- perilla seedlings, in AMF+ perilla seedlings at 3 weeks after sowing, leaf length and width increased 17% and 29%, leaf area increased 28%, and shoot fresh weight increased 33%, root total length increased 1%, and chlorophyll content increased 3%. Further at 10 weeks after sowing, compared to the AMF- perilla plants, in perilla plants inoculated with AMF at the sowing and transplanting stages, leaf area increased 21% and 19%, shoot length increased 19% and 17%, root fresh weight increased 17% and 20%, and chlorophyll content increased 5.1% and 4.8%, respectively. Moreover, at 14 weeks after sowing, compared to the AMFperilla plants, in perilla plants inoculated with AMF at the sowing and transplanting stages, the number of leaves increased 16% and 20%, root fresh weight increased 16% and 17% significantly. Further, leaf fresh weight increased 9% and 11%, shoot diameter increased 4.5% and 7.3%, and chlorophyll content increased 1.5% and 2.5%, respectively. The levels of many macronutrients and micronutrients were tended to be significantly higher in AMF+ plants than in AMF- plants, supporting the association between AMF and enhanced growth of plants grown from AMF+ seedlings.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.1
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• pp.115-120
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• 1981

Effect of soil water on the growth of Panax ginseng(2 years old) was investigated through pot experiment. the results were as follows. 1. Optimum soil moisture content for root yield appeared to be 65.5% of field capacity(22.1% fresh weight basis) and at 31.5%(10.7% fresh weight basis) relative growth rate was nil. 2. Under suboptimum condition of soil moisture, emergence of shoot and leaf unfolding was delayed. The rate of emergence of shoot and leaf area was also decreased while missing shoot rate was increased. 3. Root yield was positively correlated with leaf area per plant(r=0.91 **), stem diameter (r=0.73**), stem length(r=0.71 **) fresh top yield(r=0.93**) and negatively with missing shoot rate(r=-0.77**). 4. Fresh root weight showed negative correlation(r=-0.80**) with water content of root indicating that tissue is more compact when grown at sufficient water.

• Korean Journal of Plant Tissue Culture
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• v.25 no.1
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• pp.63-68
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• 1998

Multiple shoots obtained in MS medium suppler with 5.0 mg/L BA though shoot-tip culture. The frequency of vitrified shoot was lower on Bacto-agar medium than on Gelrite as gelling agent. Addition of activated charcoal at concentrations of 0.1~0.3% reduced vitrification and markedly increased shoot growth, and formation and growth of roots, but significantly reduced the number of shoots formed. The ratio of fresh weight to dry weight was decreased by increasing light intensity and agar concentration. Eight-tenths times of macroelement of MS medium was observed to be effective for shoot formation. Addition of IAA effectively promoted shoot formation in both shoot tip and node-bud explants. Supplement of 5.0 mg/L BA, 0.3 mg/L IAA to MS medium was most effective in shoot proliferation on shoot tip and node-bud explants.Multiple shoots obtained in MS medium suppler with 5.0 mg/L BA though shoot-tip culture. The frequency of vitrified shoot was lower on Bacto-agar medium than on Gelrite as gelling agent. Addition of activated charcoal at concentrations of 0.1~0.3% reduced vitrification and markedly increased shoot growth, and formation and growth of roots, but significantly reduced the number of shoots formed. The ratio of fresh weight to dry weight was decreased by increasing light intensity and agar concentration. Eight-tenths times of macroelement of MS medium was observed to be effective for shoot formation. Addition of IAA effectively promoted shoot formation in both shoot tip and node-bud explants. Supplement of 5.0 mg/L BA, 0.3 mg/L IAA to MS medium was most effective in shoot proliferation on shoot tip and node-bud explants.

• Journal of Korean Society of Forest Science
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• v.78 no.1
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• pp.55-60
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• 1989

To find the nutritive values of the edible bamboo shoot (Playllostachys edulis) produced in Korea, the component analysis was conducted using the fresh and canned bamboo shoot. As the principal component ; water, protein, lipid, sucrose, ash, cellulose and calorie content were measured in fresh bamboo shoot and canned bamboo shoot. Also the content of minerals ; K, P, Ca, Fe and Vitamins were analysed and compared between fresh and canned bamboo shoot. The contents of component were devided into upper, middle and bottom bamboo parts. The contents of protein, lipid, ash, cellulose and calories of raw bamboo shoot were higher than those of canned shoot. Generally, the components of bamboo shoot produced in Korea were not significantly different to those of Ueda's results in Japan. But the contents of lipid, calcium and potassium were higher in Korean bamboo shoot.

• Journal of Bio-Environment Control
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• v.16 no.3
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• pp.240-246
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• 2007

To evaluated the effect of low temperature storage on quality of fresh shoot of Sedum sermentosum, weight loss, Hunter L, a and b value, chlorophyll, water content, and general appearance were investigated during storage at 20, 10, and $5^{\circ}C$. Just after harvesting, fresh shoots ($10{\pm}2cm$ length) were stored with wrap film (linear LDPE)-packaged condition using the Styrofoam dish ($20{\times}10cm$, which 40 fresh shoots per dish). The fresh weight of 40 shoots was gradually decreased according to days after storage and higher storage temperature. The weight loss of fresh shoots showed significant difference (8.7-25.3%) between just before storage and 10 days after storage, and the degree of weight loss was more severer in higher storage temperature. Wanju local strain showed the lowest weight loss, and the most severe case was Pohang local strain. SPAD value related to chlorophyll content was rapidly decreased after 4 and 6 days at 20 and $10^{\circ}C$ storage, respectively. It was slowly decreased according to days after storage at $5^{\circ}C$. Yellowish color in bottom leaves of shoot appeared after 4, 6 and 8 days at 20, 10, and $5^{\circ}C$ storages, respectively. Hunter L and a values were gradually increased after storage, and it showed significant difference between just before storage and 10 days after storage. The total rate of discoloration over 50% or decay shoot was largely increased as higher temperature. It was significantly correlated to the water content of fresh shoot among local strains $(P{\leq}0.01)$. The freshness of shoot after storage was maintained in order of Wanji, Gunsan, Wando and Pohang local strain. The results indicated that the freshness was maintained for 8 days after $5^{\circ}C$ storage, when the fresh shoot was stored with wrap film-packaged condition.

• Journal of Korean Society of Forest Science
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• v.96 no.2
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• pp.178-182
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• 2007

Rauvolfia serpentina (L.) Benth. ex Kurz is a medicinal plant and an endangered tropical rainforest plant species. Since the field cultivation that aims to fulfill the industrial needs is never accomplished, tissue culture appears to be the most feasible way to improve the quality and quantity of R. serpentina. This experiment used two kinds of explants (roots and shoots) to induce optimal root formation in different combinations of auxin and photoperiod. Each explants exhibited different responses on given treatments. Differentiated root could be produced from explants cultured in IBA 20 mg/L with and without light. The highest number of roots, root length and root weight induced from shoot explants were effective on MS medium containing IBA 20 mg/L and incubated under dark condition, while highest total weight (callus and root) from root explants cultured on MS medium supplemented 10 mg/L IBA and 10 mg/L NAA and incubated under day length (11/13 hr). The root induced from shoot explants produced the highest major alkaloid content. The highest content of ajmaline (2.17 ppm fresh weight) and reserpine (1.30 ppm fresh weight) were observed in shoot explants cultured in MS medium containing combination of IBA 10 mg/L and NAA 10 mg/L and incubated under dark condition, yohimbine (1.47 ppm fresh weight) was in the shoot explants cultured in MS medium containing NAA 20 mg/L and incubated under day length, while serpentine was absent.