• Title/Summary/Keyword: frequency-response

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Infection Structures on the Infected Leaves of Potato Pre-inoculated with Bacterial Strains and DL-3-amino Butyric Acid after Challenge Inoculation with Phytophthora infestans

  • Kim, Hyo-Jeong;Jeun, Yong-Chull
    • The Plant Pathology Journal
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    • v.23 no.3
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    • pp.203-209
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    • 2007
  • Infection structures were observed using a fluorescence microscope at the penetration sites on the leaves of potato plants pre-inoculated with the bacterial strains Pseudomonas putida TRL2-3, Micrococcus luteus TRK2-2, and Flexibacteraceae bacterium MRL412, which mediated an induced systemic resistance on potato plants against late blight disease caused by Phytophthora infestans. In order to compare the infection structures on the leaves expressing systemic acquired resistance, the leaves of potato plants pre-treated with DL-3-amino butyric acid (BABA) were also observed after challenge inoculation with the same pathogen. The infection structures were investigated. The total number of germination and appressorium formation of P. infestans were counted. Furthermore, the frequencies of fluorescent epidermal cells at the penetration sites, which indicate a defense response of plant cell, were estimated. There were no differences on the germination rates of the fungal cysts among the untreated control, BABA pre-treated, and bacterial strains pre-inoculated plants. However, appressorium formation was slightly decreased on the leaves of BABA pre-treated plants compared to those of untreated as well as bacterial strains pre-inoculated plants. Furthermore, the frequencies of fluorescent cells of BABA pre-treated and bacterial strains pre-inoculated were higher than that of untreated plants, indicating an active defense reaction of the host cells against the fungal attack. On the other hand, the pre-treatment with BABA caused a stronger fluorescent of epidermal cells at the penetration sites compared to the pre-inoculation with the bacterial strains. Interestingly, the frequency of fluorescent cells by BABA, however, was lower than that by the bacterial strains. Based on the results it is suggested that the infection structures showing resistance reaction on the leaves of potato plants were different between by pre-inoculation with bacterial strains and by pre-treatment with BABA against the late blight pathogen.

Study on the Dynamic Characteristics of a Manual Transmission Using Linear Models (선형모델을 이용한 수동변속기의 동적 특성 연구)

  • Yoon, Jong-Yun;Lee, Iljae
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.23 no.3
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    • pp.240-248
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    • 2013
  • Torsional vibrations, such as the gear rattle of the manual transmission in vehicle systems, are correlated with the firing stroke from the engine. These vibro-impacts can be examined based upon linear time-invariant analysis. In order to understand the gear dynamics, a specific manual transmission with a front-engine front-wheel drive configuration is investigated. A method to reduce the degrees of freedom is suggested based upon the eigensolutions and frequency response functions, which will lead to the development of an efficient matrix size. The dynamic characteristics of single- and dual-mass flywheels are then compared. The effect of the dual-mass flywheel is investigated based upon the mobility analysis, which will lead to understanding of the concepts for avoiding vibro-impacts. A linear time-invariant system model is examined by employing the effective clutch stiffness from a two-stage clutch damper. Thus, the relationship between the dynamic characteristics and the clutch damper can be predicted by assuming a combination of different stage stiffness levels.

Stable Expression of TMV Resistance and Responses to Major Tobacco Diseases in the Fifth Generation of TMV CP Transgenic Tobacco

  • Park, Seong-Weon;Lee, Ki-Won;Lee, Cheong-Ho;Kim, Sang-Seock;Park, Eun-Kyung;Choi, Soon-Yong
    • Journal of the Korean Society of Tobacco Science
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    • v.20 no.1
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    • pp.66-70
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    • 1998
  • TMV resistant lines (TRLs) originated from the Blo plant of Nicotiana tabacum cv. NC82 transformed with TMV coat protein cDNA which initially showed delayed disease symptom were selected for increased resistance in each subsequent generation. The result of field experiment of the transgenic tobacco lines in the fifth generation for TMV resistance and their response to other tobacco diseases (black shank, bacterial wilt, and powdery mildew) is described in this report. When fifteen TRLs of the fifth generation were tested for TMV resistance by mechanically inoculating the individual plants, over 95 percent of the plants of 6 lines showed complete resistance even 8 weeks after the inoculation. Average frequency of the resistant plants in TRLs of the fifth generation 8 weeks after the inoculation was 87%. Stable insertion and expression of TMV coat protein cDNA in the fifth generation of the transgenic tobacco plant were confirmed by PCR and immunoblot hybridization, respectively. All TRLs were resistant to the black shank but were susceptible to the bacterial wilt disease and the powdery mildew to the same degree as non-transgenic NC82 was. Therefore, it was indicated that the phenotypes related at least to disease resistance were not changed in the transgenic tobacco. Key words : TMV CP cDNA, TMV resistant tobacco plant, transformation.

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Effect of Bupleurum falcatum on the immune system (시호 추출물이 면역계 세포의 활성에 미치는 영향)

  • Cho, Jeong-gon;Kim, Jong-myeon
    • Korean Journal of Veterinary Research
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    • v.34 no.4
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    • pp.769-779
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    • 1994
  • The root of Bupleurum falcatum L.(BF) has been widely used in oriental medicine as a major camponent in many prescriptions for chronic hepatitis, renal disease, tuberculosis and some other infectious diseases. Many attempts have done to investigate the therapeutic effects of these principles. However, any kinds of screenig on immune regulatory- and antitumor- effects of BF has not been reported. The present study, therefore, was undertaken to investigate the BF-effects on cellular- and humoral-immune responses, phagocytic activities of macrophages, lymphokine- and Immunoglobulin(Ig)-production of lymphocytes, tumorigenesis of implanted sarcoma 180 cells and B16 melanoma cells, and proliferations of some tumor cell lines(Fsa II, 3LL and EL4). BF increased phagocytic activities of mouse peritoneal macrophages in a dose- and time-dependent fashion. Arthus reaction and antibody responses to SRBC were slightly enhanced but delayed hypersensitivity was depresed when BF was injected before- and after-SRBC sensitization. BF inhibited the proliferative responses of human tonsillar lymphocytes to PHA- and Con A-stimulation but slightly augmented the response of these cells to Staphylococcus aureus Cowan 1(SAC)-activation. Ig secretion of human mononuclear cells activated with SAC was slightly increased by BF. BF significantly augmented the SAC-induced IL 6 production of human mononuclear cells but not influenced Con Ainduced IL 2 secretion. NK cell activities of mouse splenocytes were somewhat increased when BF was pretreated and this responses were due to the increment of binding affinities of effector cells to target cells and of lytic activities of effector cells against target cells. In vitro BF significantly inhibited the proliferations of cancer cells such as Fsa II, 3LL and EL4 strains. BF decreased not only the frequency of tumor induction but also the tumor size per sarcoma 180 or B16 cell-implanted mouse. Taken together, these results indicate that BF is one of the potential immunomodulator, and suggest its possibility to be used as a desirable antitumor agent.

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APPLICATION OF 3D TERRAIN MODEL FOR INDUSTRY DISASTER ASSESSMENT

  • Kim, Hyung-Seok;Cho, Hyoung-Ki;Chang, Eun-Mi;Kim, In-Hyun;Kim, In-Won
    • Proceedings of the KSRS Conference
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    • 2008.10a
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    • pp.3-5
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    • 2008
  • An increase in oil and gas plants caused by development of process industry have brought into the increase in use of flammable and toxic materials in the complex process under high temperature and pressure. There is always possibility of fire and explosion of dangerous chemicals, which exist as raw materials, intermediates, and finished goods whether used or stored in the industrial plants. Since there is the need of efforts on disaster damage reduction or mitigation process, we have been conducting a research to relate explosion model on the background of real 3D terrain model. By predicting the extent of damage caused by recent disasters, we will be able to improve efficiency of recovery and, sure, to take preventive measure and emergency counterplan in response to unprepared disaster. For disaster damage prediction, it is general to conduct quantitative risk assessment, using engineering model for environmental description of the target area. There are different engineering models, according to type of disaster, to be used for industry disaster such as UVCE (Unconfined Vapour Cloud Explosion), BLEVE (Boiling Liquid Evaporation Vapour Explosion), Fireball and so on, among them, we estimate explosion damage through UVCE model which is used in the event of explosion of high frequency and severe damage. When flammable gas in a tank is released to the air, firing it brings about explosion, then we can assess the effect of explosion. As 3D terrain information data is utilized to predict and estimate the extent of damage for each human and material. 3D terrain data with synthetic environment (SEDRIS) gives us more accurate damage prediction for industrial disaster and this research will show appropriate prediction results.

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Analysis of the dentistry-related contents uploaded on YouTube Korea (YouTube 내의 치과 관련 한국어 컨텐츠 현황 분석 및 활용 방안)

  • Jo, Jaehyun;Kwon, Hyuckjun;Jung, Seoyeon;Hu, Kyung-Seok;Jung, Il-Young;Seo, Jeong-Taeg
    • The Journal of the Korean dental association
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    • v.57 no.12
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    • pp.728-735
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    • 2019
  • Objective: Previous studies suggested the potential influence of YouTube videos regarding dentistry on the mass population. However, there was not any clear investigation for Korean population. We aimed to systemically analyze the type of the dentistry-related videos uploaded on YouTube Korea and the accounts used for uploading, and to assess their effect on the view count of the content. Methods: Classification, type of the accounts, and view count of the videos listed by the keyword 'dentistry' were analyzed, which were uploaded on YouTube Korea platform from September 2017 to April 2019. Kruskal-Wallis test with post hoc analysis was used to assess the effect of the classification of the videos and the type of accounts on the view count. Results: 1.026 videos were enrolled to the analysis. Primary classification of the videos was information/education, advertisement, life, news, child contents, autonomous sensory meridian response, broadcast, cartoon/game, humor, and music. Secondary classification of the videos was dental experience, advertisement, role-playing, information/education., humor, cartoon/game, child contents, life, and broadcast. Type of the accounts was dentistry associates, general public, media company, and government office (sorted by frequency). Subject of the most videos (93.6%) was general public. There was statistically significance in the view count of the videos according to the primary and secondary classifications, the account used for uploading, and target subject of the videos. Conclusion: Dentists and their associates should recognize the importance of YouTube platform and try to monitor and intervene the dentistry-related contents, considering its huge impact on the general public.

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The Study on the improvement of dental technician practical examination I (치과기공사 실기시험 개선에 관한 조사 연구 I)

  • Bae, Eun-Jeong;Kim, Woong-Chul;Chung, In-Sung;Nam, Sang-Yong;Kim, Ji-Hwan
    • Journal of Technologic Dentistry
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    • v.34 no.4
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    • pp.413-421
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    • 2012
  • Purpose: The purpose of this study is to investigate the improvement measure of dental technician practical examination. Methods: A survey was performed on 111 dental technicians who were self-written questionnaire. The results were analyzed by SPSS 12.0. The collected data was analyzed by frequency and chi-square test. Results: Questions most necessary work tools with the articulator was the highest with 55.9%, Electric wax carver(16.2%), heating clear(14.4%), CAD/CAM program(9.0%) was in the order. The need for additional practical test in response to actions included maxillary and mandibular comments occlusal work that you have to wax up the highest rate of 63.8%. Items most in need of improvement was strengthen of clinically oriented(48.0%), separation of written and practical(14.6%), control the degree of difficulty(11.4%) were the next order. Conclusion: Based on the results of this study, the current dental technician did not show major problems in the practical test. However, clinicians should be strengthened mainly. To do this, the use of the articulator is essential.

PLL Equivalent Augmented System Incorporated with State Feedback Designed by LQR

  • Wanchana, Somsak;Benjanarasuth, Taworn;Komine, Noriyuki;Ngamwiwit, Jongkol
    • International Journal of Control, Automation, and Systems
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    • v.5 no.2
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    • pp.161-169
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    • 2007
  • The PLL equivalent augmented system incorporated with state feedback is proposed in this paper. The optimal value of filter time constant of loop filter in the phase-locked loop control system and the optimal state feedback gain designed by using linear quadratic regulator approach are derived. This approach allows the PLL control system to employ the large value of the phase-frequency gain $K_d$ and voltage control oscillator gain $K_o$. In designing, the structure of phase-locked loop control system will be rearranged to be a phase-locked loop equivalent augmented system by including the structure of loop filter into the process and by considering the voltage control oscillator as an additional integrator. The designed controller consisting of state feedback gain matrix K and integral gain $k_1$ is an optimal controller. The integral gain $k_1$ related to weighting matrices q and R will be an optimal value for assigning the filter time constant of loop filter. The experimental results in controlling the second-order lag pressure process using two types of loop filters show that the system response is fast without steady-state error, the output disturbance effect rejection is fast and the tracking to step changes is good.

Analysis of Smart Antenna Performance Improving the Robustness of OFDM to Rayleigh Fading (레일리 페이딩 내구성을 개선시키는 OFDM 스마트안테나의 성능 분석)

  • Hong, Young-Jin
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.48 no.4
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    • pp.53-60
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    • 2011
  • In order to augment the robustness of OFDM system to Rayleigh multipath fading, there exist two smart antenna algorithms, namely, Pre-FFT smart antenna and Post-FFT smart antenna. After the mathematical modeling of both smart antenna algorithms, computer simulations have been carried to compare and analyze the performance of generalized eigen problem based Pre-FFT algorithm and the performance of Wiener solution based Post-FFT algorithm. It has been shown that the Post-FFT smart antenna far outperforms the Pre-FFT smart antenna due to the computational complexities. Especially it is so when the multipath signal arrives at beyond the guard interval and a rich co-channel interferer is introduced. Performance of a subcarrier clustering method proposed to lessen the computing load has been compared to that of a typical Wiener solution based Post-FFT smart antenna. Performance comparison between MRC(Maximum Ratio Combining) diversity based Post-FFT algorithm and typical Post-FFT algorithm has also been carried.

Decision Feedback Equalizer Based on LDPC Code for Fast Processing and Performance Improvement (고속 처리와 성능 향상을 위한 LDPC 코드 기반 결정 궤환 등화기)

  • Kim, Do-Hoon;Choi, Jin-Kyu;Ryu, Heung-Gyoon
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.23 no.1
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    • pp.38-46
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    • 2012
  • In this paper, we propose a decision feedback equalizer based on LDPC(Low Density Parity Check) code for the fast processing and performance improvement in OFDM system. LDPC code has good error correcting capability and its performance approaches the Shannon capacity limit. However, it has longer parity check matrix and needs more iteration numbers. In our proposed system, MSE(Mean Square Error) of signal between decision device and decoder is fed back to equalizer. This proposed system can improve BER performance because it corrects estimated channel response more accurately. In addition, the proposed system can reduce complexity because it has a lower number of iterations than system without feedback at the same performance. Simulation results evaluate and show the performance of OFDM system with the CFO and phase noise in multipath channel.