• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.2
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• pp.150-156
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• 1995

Planting date is the most important factor in determining winter survival of Crop plants. The objective of this study was to explore the effect of planting date on the development of freezing tolerance and winter survival of canola. Six winter cultivars were planted at three different dates during the fall on the Michigan State University Research Farm at East Lansing, MI. Freezing tolerance was determined by ion leakage tests every 15 days after planting until middle of November. Winter survival was evaluated by counting the live plants in the fall and next spring. Planting date as well as cultivar treatment had a significant effect on freezing tolerance and winter survival A different pattern in development of freezing tolerance was observed for different planting dates. There was a high correlation between freezing tolerance and winter survival suggesting that freezing tolerance could be a useful predictor for winter survival.

• Plant Resources
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• v.1 no.1
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• pp.38-42
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• 1998

One of the most attractive short-term possibilities for increasing freezing tolerance of winter crops may be the application of chemicals. This research was conducted to determine the effect of two plant growth regulators. Terpal-C and Cerone on freezing tolerance and winter survival of canola. Three cultivars were planted on the michigan state University Agronimy Farm at East Lansing. MI. on Sept. 10. 1992 and 1993 Chemicals were applied to one-month-old plants when they reached the 5 leaf stage. Ion leakage tests for freezing tolerance were conducted before and after chemical treatment. Winter survival was evaluated by counting the plant standing in the fall and spring. Neither of the chemicals. Terpal-C. inhibited natural cold hardening.

• Plant Biotechnology Reports
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• v.5 no.1
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• pp.61-69
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• 2011

The dehydration-responsive element binding proteins (DREB1)/C-repeat (CRT) binding factors (CBF) function as transcription factors and play an important role in agricultural biotechnology and molecular biology studies of drought and freezing stress tolerance. We generated transgenic Lolium perenne plants containing the PCR-cloned Arabidopsis DREB1A/CBF3 gene (AtDREB1A/CBF3) to study the function of this gene construct in drought and freezing tolerance in a species of turfgrass. Compared to the control, AtDREB1A/CBF3 transgenic L. perenne plants showed enhanced drought and freezing stress tolerance. The activities of the enzymes superoxide dismutase (SOD) and peroxidase (POD) were higher in transgenic plants than in the non-transgenic plant control. These results demonstrate that the expression of the AtDREB1A/CBF3 gene in transgenic L. perenne plants enhanced drought and freezing tolerance and that the increased stress tolerance was associated with the increased activities of antioxidant enzymes. These results are relevant to stress biology and biotechnology studies of turfgrass.

• Journal of Sericultural and Entomological Science
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• v.39 no.1
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• pp.12-16
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• 1997

Effects of calcium on mulberry growth and freezing tolerance were examined by water culture. Calcium was supplied by folar spray with the levels of 0, 5, and 40 ppm. Muberry stems developed by 130 cm at Ca2+ 40ppm, 82 cm at Ca2+ 5 ppm and 23 cm at Ca2+ 0 ppm. Muberry roots also developed vigorously at Ca2+ 40 ppm, but did poorly at Ca2+ 5 ppm and changed to brown in color, and died becoming necrosis at Ca2+ 0 ppm. Content of calcium in leaves and barks were increased at Ca2+ 40 ppm compared with at Ca2+ 5 ppm. Total sugar, RNA, proline and phospholipid at Ca2+ 40 ppm were also more increased than those at Ca2+ 5 ppm. Mulberry stems grown at Ca2+ 40 ppm showed a sufficient tolerance at -10 for 24 hours while stems grown at Ca2+ 5 ppm did a weak tolerance at the same conditions.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.5
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• pp.407-412
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• 2003

In order to measure an antifreezing tolerance, antifreeze proteins accumulated upon cold acclimation in apoplast were analyzed. As Dongborilho were cold-acclimated for 3 to 74 days there was an abrupt increase in apoplastic proteins up to 30 days and then decrease to the similar levels. Among the known antifreeze proteins, CLP produced in E. coli. and TLP purified from apoplast were used to generate antisera that allow to measure and localize the proteins in leaves of barley. The CLP of 27.7 kDa and TLPs of 6, 26, 27, 30, and 31 kDa were increased in their amounts in apoplast as cold treatment being longer. There was a correlation among the amounts of those proteins accumulated in apoplast and freezing tolerance as shown in field and ion leakage tests for five cultivars. The deposit of CLP was localized in the marginal area and the area adjacent to leaf vescular bundle cells in an increasing manner according to duration of cold acclimation but no variation was observed in terms of it's distribution. Based on the close correlation between levels of antifreeze proteins and degrees of freezing tolerance, the immunological methods was to develop to estimate a freezing tolerance in barley.

• Journal of Sericultural and Entomological Science
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• v.22 no.1
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• pp.7-25
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• 1980

Some substances and freezing tolerance in the mulberry (Morus species) branch have been studied on the basis of varietal differences and harvesting times along with harvesting methods in autumn. The results obtained are summarized as follows; 1. The highest freezing tolerance was shown in the varieties of Yongcheon-chou, Jasan, Kang-weon No. 3 and Ichihei, the medium in Roso. Kairyonezumigaeshi, Yanagida and Kokuso No. 28, and the lowest in Ichinose, Mokuso, Kokuso No. 21 and Suweousang No. 3. 2. There was a signifiant negative correlation (r= -0.59＊) between death atop percentage in the field and the temperature required to kill 50% of the mulberry buds (T$_{50}$) with the harvesting times and methods in autumn. Cold hardening occurred in the early through the end of September with the peak at the mid-september. During this period, leaf harvest decreased freezing tolerance with remarkable decrease due to picking all the leaves and leaving several leaves at the base of branch. Greater cold hardening was induced by leaving several leaves after topping. 3. Negative correlations were observed between freezing tolerance and the contents of soluble (r =-0.70＊) and crude (r= -0.70＊) protein. However, positive correlations were shown between freezing tolerance and total carbohydrate contents per crude (r=0.31＊) and per soluble (r=0.71＊) protein . There were also positive correlations between freezing tolerance and total sugar (r=0.67＊) and RNA content (r=0.99＊＊). No relationships of dry matter. fat. total carbohydrate and DNA contents were observed to the freezing tolerance. 4. Such sugars as raffinose. lactose, sucrose, glucose, fructose. arabinose. xylose. ribose (assumed) and rhamnose were detected in winter mulberry branch. Major sugars such as sucrose, glucose, and fructose were supposed to have higher relationship to the freezing tolerance than the other sugars. 5. Late harvesting increased RNA content except in the case of total leaf picking at mid-September. Leaf picking decreased RNA content. Some amount of RNA was, however, maintained by leaving several leaves after topping Leaving upper-middle leaves of a branch showed high RNA content. Leaving young leaves at the top and the overmatured leaves at the base showed low content. A positive correlation (r=0.51＊) was noted between RNA content and freezing tolerance in the different harvesting methods.s.

• BMB Reports
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• v.30 no.1
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• pp.21-25
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• 1997

The growth. freezing resistance and electrophoretic protein patterns of carrot callus cultures were investigated following treatment with NaCl for various' intervals at 20$^{\circ}C$. Following 7 day exposure to 250 mM NaCl. freezing tolerance increased, which was measured by 2.3.5-triphenyl tetrazolium chloride (TTC) assay and fresh weight was reduced compared to control cells. Changes of electrophoretic patterns of total and boiling stable proteins were investigated using one or two dimensional gel system. Several proteins with molecular weight of 43 and 21 kDa increased by NaCl treatment. The most prominent change was detected in 21 kDa protein. The steady state level of this protein increased in NaCl treated cells, but decreased in control cells. Twenty one kDa protein was detected only in the NaCl treated cell when boiling stable protein was analyzed. The isoelectric point of 21 kDa protein was identified as 5.7. The timing of increase of 21 kDa protein was correlated to freezing resistance which implied the role of this protein in the induction of freezing resistance of the cell.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.357-363
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• 2018

The objective of this study was to investigate whether exogenous proline (Pro) could confer freezing tolerance of spinach and determine fluctuations of free amino acids in spinach leaf tissues under freeze-induced stress. Treatment with Pro (10 mM) resulted in more accumulation of Pro (~2.6-fold) in Pro-treated spinaches compared to untreated ones. These Pro-pretreated spinaches were more freezing-tolerant, showing more turgid leaves and petioles compared to untreated controls. However, when spinaches pre-treated with or without Pro were subjected to freezing, there was no significant difference in overall amino acid contents, emphasizing the role of Pro as an osmoprotectant. Freezing stress prompted intensification of total amino acid contents irrespective of pretreatment with Pro. Asp, Glu, Ala, and Val were the most abundant free amino acids due to increased protein degradation and nitrogen mobilization for plant survival under freezing stress. Arg, a precursor for the synthesis of polyamines in plants, was profoundly enhanced under freezing stress. This implies that Arg plays an important role in modulating freezing tolerance. Gly, Leu, and Ile were maintained at relatively low levels in all treatments. However, Ser, Tyr, and Lys as primary constituents of dehydrins were accumulated under freezing stress, suggesting that they might play a role in increasing cryoprotective activity under freezing stress.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.04a
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• pp.170-170
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04b
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• pp.153-153
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• 2002