• Title/Summary/Keyword: freezing storage

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Changes in the $Ca^{2+}\;and\;Mg^{2+}$ - dependent Adenosine Triphosphatase Activity and Ultrastructure of Marine Fishes by Partial Freezing III. Changes in the Ultrastructure of Muscle Tissues of Yellowtail during Low-temperature Preservation (a해산어의 부분동결에 의한 $Ca^{2+}\;및\;Mg^{2+}$ -dependent Adenosin Triphosphatase 활성 및 근섬유의 미세구조 변화 III. 저온저장 과정중 방어 근육조직의 미세구조의 변화)

  • 최경호;박찬성
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.6
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    • pp.629-636
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    • 1991
  • Yellowtail fishes(Seriola quinqueeradiata) were submitted to the storages using ice-cooling($0^{\circ}C$), partial freezing($-3^{\circ}C$) and freezing $-20^{\circ}C$) method. Changes in the structures of muscle during storage at different temperatures were investigated. The ice-cooling and partial freezing storage caused early decomposition of glycogen granules and mitochondrial inner membrane, but it was accorded to much slower manner comparing with that of ice-cooling storage. The scars of ice crystals were appeared after three days of storage. The number and size of the crystal increased as progressing of the storage. They were circular and mostly located between fibers. When using the freezing storage, glycogen granules were mostly found from the muscle cell even after fourteen days of storage. Mitochonidral inner membrane maintained their integrity. The scars of ice crystals were also found, however, different from those of partial freezing storage. Their existing sites were random and their shapes were irregular. In many cases, they located in the fiber and had keen edges. Fibers were broken mostly at the Z-lines on fourteen days of storage. From these results, it was concluded that partial freezing storage can repress autolytic enzymic action and can reduce the physical damage from ice crystals which is caused by freezing.

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Mirror-Switching Scheme for High-Speed Embedded Storage Systems (고속 임베디드 저장 시스템을 위한 복제전환 기법)

  • Byun, Si-Woo;Jang, Seok-Woo
    • Transactions of the Society of Information Storage Systems
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    • v.7 no.1
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    • pp.7-12
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    • 2011
  • The flash memory has been remarked as the next generation media of portable and desktop computers' storage devices. Their features include non-volatility, low power consumption, and fast access time for read operations, which are sufficient to present flash memories as major data storage components for desktop and servers. The purpose of our study is to upgrade a traditional mirroring scheme based on SSD storages due to the relatively slow or freezing characteristics of write operations, as compared to fast read operations. For this work, we propose a new storage management scheme called Memory Mirror-Switching based on traditional mirroring scheme. Our Mirror-Switching scheme improves flash operation performance by switching write-workloads from flash memory to RAM and delaying write operations to avoid freezing. Our test results show that our scheme significantly reduces the write operation delay and storage freezing.

Storage Characteristics of Rough Rice by Storage Method (저장방법에 따른 벼의 저장특성)

  • Lee, J.S.;Hong, H.K.;Kang, T.H.;Li, H.;Ham, T.M.;Kim, Y.H.;Han, C.S.
    • Journal of Biosystems Engineering
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    • v.33 no.3
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    • pp.167-172
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    • 2008
  • The objective of this study was to investigate the adequate storage method that was able to maintain the high quality of rough rice according to storage methods and period. The quality change of the rough rice during the storage period was evaluated by storage method such as cooling bin using winter cold air, ordinary temperature bin, freezing, refrigeration and indoor storage. Moisture content, brown rice whiteness, hardness, crack ratio and germination ratio were measured in this study. Moisture content of rough rice stored in cooling bin using winter cold air and ordinary temperature bin were decreased by 0.07% and 0.42%, respectively, which were lower than the other storage method. The hardness of brown rice increased in order of storage method such as winter cooling bin, normal bin, freezing storage, refrigeration storage and indoor storage. Crack ratio by indoor and ordinary temperature bin storage were increased by 2.68% and 3.63%, respectively, whereas cooling bin using winter cold air, refrigeration and freezing storage showed below 1.0%. The highest germination rate was found in cooling bin using winter cold air. As a result, cooling bin using winter cold air can be evaluated for the adequate storage method of rough rice.

Changes in Quality during Frozen Storage of Meat with Thermal Equalized Freezing (균온처리 동결에 의한 식육의 저장중 품질변화)

  • Jeong, Jin-Woong;Lee, Ho-Jun;Park, Noh-Hyun
    • Korean Journal of Food Science and Technology
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    • v.31 no.3
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    • pp.688-696
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    • 1999
  • Changes in quality during frozen storage of meat with thermal equalized freezing and various freezing methods were investigated. When beef were frozen at freezing rate of $0.39{\sim}0.66\;cm/h$, average diameter of ice crystal were about $30{\sim}50\;{\mu}m$ and showed broken tissues or irregular cracks. At freezing velocity of $1.14{\sim}2.26\;cm/h$, ice crystals of about $10{\sim}30\;{\mu}m$ was formed mainly inside or between fiber and slight destruction of tissues was occurred. The average diameter (D) of the ice crystals were related to the characteristic freezing time $(t_c)$ by the equation: $D({\mu}m)=4.089+26.88logt_c\;(r^2=0.913)$. Beef with still-air freezing showed higher drip loss than methods of immersion and thermal equalized freezing. Also, drip loss of pork was relatively lower than beef and showed highest value to 7.39% during storage on 40 days at air-blast freezing method. No apparent change of pH during storage of frozen beef and pork by freezing methods were detected. However, least changes for sample with thermal equalized freezing was found compare to sample with still-air and air blast freezing in VBN and TBA value. The fluctuation of frozen storage temperature did not cause noticeable changes on pH and water content. However, drip loss, VBN and TBA values were increased slowly as frequency of fluctuation increased.

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Short Term Storage and Cryopreservation of Trumpet Shell Charonia sauliae Sperm

  • Kang, Kyoung-Ho;Seon, Seung-Chun;Zhou, Bin
    • The Korean Journal of Malacology
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    • v.24 no.1
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    • pp.51-57
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    • 2008
  • With the purpose to estimate the possibility of short-term storage and cryopreservation for sperm of Charonia sauliae, which is a potential preparation for its artificial reproduction and further research, in this study, protocols for short-term storage and cryopreservation of trumpet shell sperm was optimized. The effects of different immobilizing solutions, dilution ratios were estimated for short-term storage. And the effects of different cryoprotectant extenders and freezing rates were estimated for cryopreservation in terms of motility and survival of sperm. The results indicated that the artificial sea water of 350 mOsmol/kg is a better immobilizing solution and sperm which was diluted at a ratio of 1:1 (v/v) had higher motility and survival rate during short-term storage. The effect of 5% dimethyl sulfoxide was significantly better than those of other cryoprotectant extenders. And a freezing rate of $-20^{\circ}C\;min^{-1}$ showed better effect than other freezing rates. In conclusion, this study optimized some key factors of the short-term and cryopreservation of C. sauliae sperm, which can provide valuable data for germ-plasm conservation and artificial propagation of C. sauliae.

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Quality Characteristics of Makgeolli during Freezing Storage (냉동저장에 따른 막걸리의 품질특성)

  • Lee, Jin-Won;Shim, Jae-Yong
    • Food Engineering Progress
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    • v.14 no.4
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    • pp.328-334
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    • 2010
  • Recently the enhancement and development of makgeolli processing to extend shelf life are constantly accomplished. However, the standardization to restrict microorganisms including cold chain system and sterilizing system has not been established yet. Therefore, the objective of this study was to investigate the storage stability of makgeolli using quick freezing (QF) and slow freezing (SF) storage methods. The storage period was 40 days. Every 10 days, the samples were taken from the quick and slow freezing storage chamber. And then the samples were put into a $10^{\circ}C$ refrigerator for 24 hr to thaw them. The final samples were evaluated for chemical experiments and microbial cell counts. As a result, reducing sugar content was dramatically increased after 10 days for all of the samples. In titratable acidity and color values case, these values did not significantly change by storage time. In case of lactic acid bacteria and yeasts for all the samples, there was a decreasing tendency with storage time. Especially, in case of lactic acid bacteria, the changes from the beginning microbial cell counts ($4.1{\times}10^7$ CFU/mL) for QF and SF after 20 days were $3.6{\times}10^6$ CFU/mL and $1.8{\times}10^4$ CFU/mL, respectively. This result showed that the freezing methods could restrict the microbial growth in makgeolli.

Effect of Initial Freshness of the Japanese Spotted Mackerel on Freezing Storage of the Fish (점고등어의 초기선도(初期鮮度)가 동결저장(凍結貯藏)에 미치는 영향(影響))

  • Park, Chan-Sung;Choi, Kyoung-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.3
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    • pp.213-221
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    • 1986
  • Japanese spotted mackerels(Scomber tapeinoephalus) were stored at $0^{\circ}C$ for 7days as the maximum, and then, they were stored again at $-3.5^{\circ}C$(partial freezing) or $-20^{\circ}C$(freezing), respectively. During storage by partial freezing, the fishes with a longer period of prestorage at $0^{\circ}C$ showed an earlier increase in the number of bacterial cells on their skin, however, it was not apparent in the freezing storage. K value of the fishes with 7 days of prestorage increased from 20% to 65.4% for 36 days of partial freezing storage, that of the fishes prestored for 0 and 4 days were 39.9% and 53.2% respectively. On contrastly, no drastic increase in K value was observed in the fishes of freezing storage. Content of volatile nitrogen of the fish muscle prestored for 4 days gradually increased from 10mg% to 29.4mg% and 17.2mg% during 36 days of partial freezing and 83 days of freezing storage respectively, that of the fishes with 7 days of prestorage showed no significant increase, moreover, it was decreased within early period of both of the storages. Free drip from the fishes with partial fleering was higher almost 5 times than that from the fishes with freezing, the highest free drip was observed from the fishes with 4 days of prestorage.

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Effects of Freezing Rate and Storage Temperature on the Degree of Retrogradation, Texture and Microstructure of Cooked Rice (동결속도 및 저장온도가 취반된 쌀의 노화도, 조직감 및 미세구조에 미치는 영향)

  • Choi, Sung-Gil;Rhee, Chul
    • Korean Journal of Food Science and Technology
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    • v.27 no.5
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    • pp.783-788
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    • 1995
  • Cooked rices were frozen at four different rates(3, 5, 7 and 12 hr) of maximum ice crystal formation zone and stored at $-20^{\circ}C\;and\;-70^{\circ}C$ for 3 months. Freezing rate, storage temperature and storage period all affected the degree of retrogradation of cooked rice. As the maximum ice crystal formation zone increased from 3 hrs to 12 hrs, the degree of retrogradation of cooked rice increased from 14.9% to 40.0%. Further retrogradation occurred during the freezing storage and cooked rice stored at $-20^{\circ}C$ retrograded faster than that held at $-70^{\circ}C$. The hardness and adhesiveness of frozen cooked rice thawed in $40^{\circ}C$ water were measured. Hardness of the frozen cooked rice was higher than that of non-frozen sample and was higher at lower freezing rate. However, the hardness of cooked rice decreased after 3 months of storage. On the other hand, the adhesiveness decreased during the freezing processing, and adhesiveness decreased more rapidly at a higher freezing rate. However, the adhesiveness of cooked rice increased after 3 months of the storage, and the level of decrease was higher at $-70^{\circ}C$ than at $-20^{\circ}C$. After 3 months of storage, ice crystal size of frozen cooked rice became larger by recrystalization than that of frozen sample prior to storage. Microstructure of cooked rice was damaged by ice crystal formation and its growth when observed by scanning electron microscope.

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EFFECT OF DIFFERENT STORAGE TEMPERATURES (INCLUDE CONTROL FREEZING POINT AND PARTIALLY FREEZING STORAGE) ON FLAVOR AND ATP-RELATED COMPOUNDS OF PORK LOIN CHOPS

  • Lin, L.C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.3
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    • pp.417-422
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    • 1993
  • This study was designed to determine the changes of ATP-related compounds, especially the concentration of IMP, and compares the relationship between IMP and flavor of pork loin chops during storage as three different storage temperatures (include $4^{\circ}C$ CF and PF). Pork loin chops were kept under $4^{\circ}C$ $-1.5{\pm}0.5^{\circ}C$ (control freezing storage) and $-3{\pm}0.5^{\circ}C$ (partially freezing storage). The changes of TBA values, ATP-related compounds, pH values, in CF stored samples were higher than PF stored samples, but it had lower TBA values than $4^{\circ}C$ storage. The IMP concentration reached their peak after 2 days in $4^{\circ}C$, 5 days in CF and 7 days in PF storage, and the ATP, ADP, AMP contents of the loin chops showed minimum, respectively. Flavor of meat sensory score for $4^{\circ}C$ stored samples were more intense (p<0.05) than CF and PF samples on day 2 of storage. However, after storage for 5 days, flavor scores for CF samples were more intense (p<0.05) than $4^{\circ}C$ and PF samples. Flavor scores for PF samples were more intense (p<0.05) than $4^{\circ}C$ and CF on day 7 of storage. As the meat with the peak of IMP contents was most preferred, it was considered that the content of IMP was related to the flavor of meat and that CF, PF had influence on the IMP content.

The Effect of Freezing Rates on the Physico-Chemical Changes of Beef during Frozen Storage at $-20^{\circ}C$ (동결속도에 따른 쇠고기의 냉동저장중 이화학적 변화)

  • Kim, Young-Ho;Yang, Seung-Yong;Lee, Moo-Ha
    • Korean Journal of Food Science and Technology
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    • v.20 no.3
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    • pp.447-452
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    • 1988
  • In order to study effect of freezing rates on the quality changes such as pH, TBA value, free fatty acids and protein extractability, cylindrical chopped beef logs with 10cm of diameter and 10cm of height were frozen at three freezing rates(0.97cm/hr, 2.05cm/hr, 3.71cm/hr)using air blast freezer. Physicochemical changes of frozen meat were investigated during forzen storage at $-20^{\circ}C$ for 16weeks. Results on pH change showed $0.1{\sim}0.2unit$ increase at the 16th week of the frozen storage and the change was smaller with the increasing freezing rates. Free fatty acids content and TBA value also were increased during forzen storage, but they were minimal at 3.71cm/hr freezing rate. Correlation coefficient between TBA value and free fatty acids content were highly significant(r=0.804). After 16weeks of storage, extractibilities of salt soluble protein were decreased by 17.7%, 6.1% and 1.6% at freezing rates of 0.97, 2.05 and 3.71cm/hr, respectively. On the other hand, extractabilities of water soluble protein were decreased by 26.0%, 21.2% and 18.5%, respectively. The effect of freezing rates on the protein extractability appeared to be greater in salt soluble protein than in water soluble protein, but freezing denaturation was more rapid in water soluble protein.

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