• 한국해양환경ㆍ에너지학회지
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• 제14권4호
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• pp.301-306
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• 2011

저온 해수에너지를 이용한 난방사이클 및 이와 연계할 수 있는 적정 담수화사이클에 대하여 제안 및 해석을 수행하였다. 난방사이클은 기본 증기압축식 사이클을 이용하여 1단 및 2단압축사이클에 대해 해석을 수행하였고, 담수화사이클은 난방사이클과 연계할 수 있도록 설계하여 해석을 수행하였다. 난방사이클의 증발기 열원으로 5이하의 저온 해수열을 이용하였고, 난방수 온도는 60 이상 공급될 수 있도록 해석하였으며, 사이클 작동유체는 R-134a, R-1234 yf, R-600a를 적용하였다. 사이클 해석 결과, 2단압축사이클을 적용할 경우 1단압축사이클에 비해 모든 냉매에서 압축기 소요동력은 약 15.6% 감소하였고, 성능계수는 약 17.6% 향상되었다. 냉동법 담수화 사이클의 경우 R-134a에 대해서 난방수 60 조건일 때, 1단압축 사이클에 비해 2단압축사이클을 적용할 경우 담수 1kg 생산당 에너지가 약 19.8% 감소하는 것으로 나타났다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2003년도 가을 학술발표회 논문집
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• pp.54-57
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• 2003

Scaling deterioration of port concrete structures due to the combined effects of chemical actions by seawater and the freeze-thaw action is also a problem which has not yet been fundamentally solved. Furthermore, deterioration of concrete surface was considered as accelerate factor of concrete durability tended to decrease. Therefore, we considered the scaling measuring method and decreasing influence of durability of concrete according to kind of binders, such as OPC, Slag, Slag+Fa, due to freeze and haw of sea water. As a results of this study, it was effective method of scaling deterioration of concrete, and confirmed the salt deterioration resistance effect to use slag binder against to sea water.

• 한국구조물진단유지관리공학회 논문집
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• 제7권2호
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• pp.141-148
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• 2003

Concrete surface is a part that resists chiefly to carbonation by carbonic acid gas, affluence of rainwater and air, deterioration by freezing thawing, salt damage by seawater, and other chemical erosion etc. Therefore, this research analyzed physical characteristic and durable characteristic of concrete eliminated glut water and bubble of concrete surface using CON-SILK that is permeated with dehydration. As a research result, concrete using CON-SILK was improved, in comparison with concrete using general formwork, in physical performance and durability of concrete, as surface hardness, carbonation resistivity, and salt content permeation resistivity etc. Therefore, we could know that it is effective to use CON-SILK in performance elevation of concrete surface.

• Ocean and Polar Research
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• 제25권4호
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• pp.607-615
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• 2003

Many organisms are known to survive in icy environments. These include both over wintering terrestrial insects and plants as well the marine fish inhabiting high latitudes. The adaptation of these organisms is both a fascinating and important topic in biology. Marine teleosts in particular, can encounter ice-laden seawater that is approximately $1^{\circ}C$ colder than the colligative freezing point of their body fluids. These animals produce a unique group of proteins, the antifreeze proteins (AFPs) or antifreeze glycoproteins (AFGPs) that absorb the ice nuclei and prevent ice crystal growth. Presently, there are at least four different AFP types and one AFGP type that are isolated from a wide variety of fish. Despite their functional similarity, there is no apparent common protein homology or ice-binding motifs among these proteins, except that the surface-surface complementarity between the protein and ice are important for binding. The remarkable diversity of these proteins and their odd phylogenetic distribution would suggest that these proteins might have evolved recently in response to sea level glaciations just 1-2 million years ago in the northern hemisphere and 10-30 million years ago around Antarctica. Winter flounder, Pleuronectes americanus, has been used as a popular model to study the regulation of AFP gene expression. It has a built-in annual cycle of AFP expression controlled negatively by the growth hormone. The signal transduction pathways, transcription factors and promoter elements involved in this process have been studied in our laboratory and these studies will be presented.

• 한국패류학회지
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• 제20권1호
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• pp.17-26
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• 2004

In present study, attempts were made to preserve abalone (Haliotis discus hannai) sperm in liquid form at low temperature, to evaluate the effect of various diluents in short-term storage on sperm, and cryopreservation procedures were optimized for the cryoprotectants and freezing rates, as well as the motility, survival rate, and the ultrastructural changes of sperm after short-term storage and cryopreservation were observed. The abalone sperm reached maximum motility until about 4 min after activation. The motility was constant for about 16 min, after which it dropped gradually, and about 50 min later all motility ceased. In Hanks' balanced salt solution (HBSS, 300 and 400 mOsmol/kg) and 150, 250 and 350 mOsmol/k artificial seawater (ASW), the sperm was immotile. After 100% ASW was added, motility of those sperm, which are in 300, 400 mOsmol/kg HBSS, 250, 350 mOsmol/kg ASW, could be again restored incompletely. Sperm motility can be maintained for 20 days of cold storage only in ASW of 850 and 1200 mOsmol/kg. A high motility index of 3.5-4.5 was observed for the first 8 days in 850 and 950 mOsmol/kg ASW. In other diluents sperm motility was constant less than 10 days, and the motility index was obviously lower than that of sperm in 850 and 1200 mOsmol/kg ASW. After 20 days of cold storage, survival rates of 10.2%-20.7% were obtained in ASW and 300 mOsmol/kg HBSS, and that in 400 HBSS (65.3%) was significantly higher than others. The constant period of sperm motility stored in 850 mOsmol/kg ASW was obviously longer than that in 1200 mOsmol/kg ASW after 6 days of storage. The sperm plunged into liquid nitrogen all died except that sperm using 15% glycerol as cryoprotectant restored 10.4% of motility. The highest motility index (3.4) was obtained with 5% glycerol and freezing procedure: $-50^{\circ}C$/min from $20^{\circ}C$ to $-80^{\circ}C$.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2016년도 추계학술대회
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• pp.663-664
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• 2016

해수를 이용한 온도차 발전은 심층수와 표층수의 온도 차이를 이용한 암모니아(냉매)를 사용하여 폐쇄적인 액화와 기화를 반복하여 터빈을 돌리는 방식이다. 온도차 발전의 특성상 온도 차이가 클수록 에너지 발전 특성은 좋아진다. 이는 심층수 표층수의 온도차이가 커야 됨을 이야기 한다. 하지만 겨울이 되면 표층수와 심층수의 온도차이가 크지 않아 에너지 효율이 떨어지는 문제점이 있다. 그리고 해수 담수화기술은 지구의 97%를 차지하지만 우리가 먹을 수 없는 바닷물을 담수로 바꾸는 기술로, 해수 담수화기술로 생산된 담수는 선박내의 공업, 식수 등 각종 용수로 사용할 수 있다. 본 논문에서는 현재 문제가 되고 있는 계절에 따른 표층수의 온도 변화를 개선하기 위해 기존에 사용하고 있는 선박용 디젤엔진의 열에너지와 LNG의 냉열 에너지를 사용한 온도차 발전을 위해 LNG 운송 선박을 기준으로 LNG 운송 선박 하이브리드 엔진에 관한 연구와 냉열 에너지를 활용한 해수 냉동법으로 담수화하는 발전 및 담수화를 복합시킨 연구를 제시하고자 한다.

• 한국해양공학회지
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• 제35권5호
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• pp.369-381
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• 2021

The International Ice Patrol (IIP) was established after the Titanic collided with an iceberg off the eastern coast of Canada in 1912 and sank, killing more than 1,500 people. Recently, the IIP has analyzed satellite images and provided safe operation information to vessels by tracking the occurrence and movement of icebergs. A large number of recent arctic studies mainly deal with sea ice formed by freezing seawater related to sea routes and resource development. The iceberg that collided with the Titanic was land-based ice that dislodged from a glacier and fell into the sea. The properties of these two types of ice are different. In addition, vessels operating in ice-covered waters such as the Arctic sea have an ice-breaking function or minimum ice-strengthened functions. Ships operating on transatlantic routes including the eastern coast of Canada do not necessarily require ice-strengthened functions. Hundreds to thousands of icebergs are discovered each year near the area where the Titanic sank. In this study, the status of ship-iceberg collision accidents was investigated to provide useful information to researchers, and the physical and mechanical characteristics of icebergs were investigated and summarized.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.74-74
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• 2003

In present study, attempts were made to preserve abalone (Haliotis discus hannai) sperm in liquid form at low temperature, to evaluate the effect of various diluents in short-term storage on sperm, and cryopreservation procedures were optimized for the cryoprotectants as well as freezing rates, in terms of the motility and survival rate, and the ultrastructural changes of sperm after short-term storage and cryopreservation were observed. The abalone sperm reached maximum motility until about 4min after activation. The motility was constant for about 16min, after which it dropped gradually, and about 50min later all motility ceased. Threshold activation of sperm was found in 40% artificial seawater (ASW), and motility increased as the concentration of ASW increased. In Hanks balanced salt solution without calcium (Ca-Free HBSS, 300 and 400 mOsmol/kg) and 10%, 20%, and 30% ASW the sperm was immotile, and motility once again restored incompletely only in HBSS of 300 and 400 mOsmol/kg, 20% and 30% ASW after 100% ASW was added. Sperm motility was extended following 20 days of cold storage only in 70% and 100% ASW. A high motility index of 3.5-4.5 was observed for the first 8 days in 70% and 80% ASW. In other diluents sperm motility was constant less than 10 days, and the motility index was obviously lower than that of sperm in 70% and 100% ASW. After 20 days of cold storage survival rates of 10.2%-20.7% were obtained in ASW and 300 mOsmol/kg HBSS, and that in 400 HBSS (65.3%) was significantly higher than others. The constant period of sperm motility stored in 70% ASW was longer obviously than that in 100% ASW after 6 days of storage, and the time to maximum motility of sperm stored in 70% increased gradually, while the difference in which of sperm in 100% ASW was not significant. The sperm plunged into liquid nitrogen all died except that sperm using 15% glycerol as cryoprotectant restored 10.4% of motility. The highest motility index (3.4) was obtained with 5% glycerol and freezing procedure: $50^{\circ}C$/min from $20^{\circ}C$ to $-80^{\circ}C$.

• 한국수정란이식학회지
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• 제31권4호
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• pp.355-365
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• 2016

The cryopreservation of sperm has become the subject of research for successful artificial insemination technologies. Antifreeze proteins (AFPs), one of the factors necessary for effective cryopreservation, are derived from certain Antarctic organisms. These proteins decrease the freezing point of water within these organisms to below the temperature of the surrounding seawater to protect the organism from cold shock. Accordingly, a recent study found that AFPs can increase the motility and viability of spermatozoa during cryopreservation. To evaluate this relationship, we performed cryopreservation of boar sperm with AFPs produced in the Arctic yeast Leucosporidium sp. AFP expression system at four concentrations (0, 0.01, 0.1, and $1{\mu}g/ml$) and evaluated motility using computer assisted sperm analysis. DNA damage to boar spermatozoa was measured by the comet assay, and sperm membrane integrity and acrosome integrity were evaluated by flow cytometry. The results showed that motility was positively affected by the addition of AFP at each concentration except $1{\mu}g/ml$ (p<0.001). Although cryopreservation with AFP decreased the viability of the boar sperm using, the tail DNA analyses showed that there was no significant difference between the control and the addition of 0.1 or $0.01{\mu}g/ml$ AFP. In addition, the percentage of live sperm with intact acrosomes showed the least significant difference between the control and $0.1{\mu}g/ml$ AFP (p<0.05), but increased with $1{\mu}g/ml$ AFP (p<0.001). Our results indicate that the addition of AFP during boar sperm cryopreservation can improve viability and acrosome integrity after thawing.

• 한국양식학회지
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• 제11권1호
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• pp.67-75
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• 1998

감성돔 (Acanthopagrus schlegeli) 정자의 냉동 보존시 정자의 생리활성과 보존효과를 비교하기 위하여, 순환여과 사육시스템에서 사육한 전장 $25.9{\pm}1.7$ cm, 체중$292.8{\pm}53.7$ g의 성어로부터 얻은 정액을 재료로 하여 희석액별, 동해방지제별 해동후 정자의 활성, 생존율 및 알에 대한 수정능력을 평가하였다. 희석액별 냉동보존에서 해동정자의 생존율은 3% sodium citrate에서 $80{\pm}1.4$%로 가장 높았으나, 수정률은 5.4% glucose에서 $63.4{\pm}4.4$%로 가장 높았다. 동해방지제별 냉동보존에서 해동정자의 수정률은 5~15% glycerol을 동해방지제로 사용하였을 때, 50.1~69.4%로 DMSO 보다 나은 효과를 보였다. 감성돔 정자의 냉동보존을 위한 적정 희석액과 동해방지제는 5% glucose와 5% glycerol이었다. 냉동하지 않은 감성돔 정자의 머리는 구형으로 직경 $1.5{\pm}0.1$ ${\mu}$m, 길이 $1.3{\pm}0.1$${\mu}$m였으며, 치밀한 핵질로 채워져 있었다. 편모는 전형적인 9+2 구조를 나타냈다. 그러나 냉동후 해동시킨 정자중에서는 염색질의 과립화, 세포막의 이탈에 의한 그 용적이 커지는 구조변화를 보였다.