• International Journal of Industrial Entomology and Biomaterials
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• 제15권1호
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• pp.23-33
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• 2007

A simple and reliable cryo technique using desiccation and slow freezing of winter dormant buds was employed for 238 core collection of mulberry germplasm collected from diverse geographical regions and maintained under tropical conditions in the ex situ field gene bank to develop long-term biodiversity conservation for ensuring sustainable utilization of these valuable resources. Desiccation and freezing tolerance of bud grafts and excised shoot apices in the axillary buds of different Morus species under in vivo and in vitro condition indicated species-specific variation and most of the wild Morus species were found sensitive. In vitro regeneration and cryopreservation($-196^{\circ}C$) protocols using differentiated bud meristem like axillary winter dormant buds were worked out for a wide range of Morus species, land races, wild and cultivated varieties. Successful cryopreservation of mulberry winter dormant buds of different accessions belonging to M. indica, M. alba, M. latifolia, M. cathayana, M. laevigata, M. nigra, M. australis, M. bombycis, M. sinensis, M multicaulis and M. rotundiloba was achieved. Among wild species Morus tiliaefolia, and M. serrata showed moderate recovery after cryopreservation. Survival rates did not alter after three years of cryopreservation of different Morus species. ISSR markers were used to ascertain the genetic stability of cryopreserved mulberry, which showed no difference detected among the plantlets regenerated from frozen apices in comparison to the non-frozen material.

• 한국분말재료학회지
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• 제23권1호
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• pp.49-53
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• 2016

The present study demonstrates the effect of freezing conditions on the pore structure of porous Cu-10 wt.% Sn prepared by freeze drying of $CuO-SnO_2$/camphene slurry. Mixtures of CuO and $SnO_2$ powders are prepared by ball milling for 10 h. Camphene slurries with 10 vol.% of $CuO-SnO_2$ are unidirectionally frozen in a mold maintained at a temperature of $-30^{\circ}C$ for 1 and 24 h, respectively. Pores are generated by the sublimation of camphene at room temperature. After hydrogen reduction and sintering at $650^{\circ}C$ for 2 h, the green body of the $CuO-SnO_2$ is completely converted into porous Cu-Sn alloy. Microstructural observation reveals that the sintered samples have large pores which are aligned parallel to the camphene growth direction. The size of the large pores increases from 150 to $300{\mu}m$ with an increase in the holding time. Also, the internal walls of the large pores contain relatively small pores whose size increases with the holding time. The change in pore structure is explained by the growth behavior of the camphene crystals and rearrangement of the solid particles during the freezing process.

• Clinical and Experimental Reproductive Medicine
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• 제26권2호
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• pp.127-135
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• 1999

Cryopreservation is able to store the surplus pre-embryos for freezing and furthermore thawing and transfer in a subsequent cycle. Cryopreserving cells which are maintaining their viability are the very complex process. This study has been carried out in order to find the effects of cryopreservation steps, freezing media and embryonic stages on the rates of viability and development of cryopreserved mouse embryos. Female ICR mice ($6{\sim}8$ weeks old) were induced to superovulate by sequential intraperitoneal injection of 5 IU PMSG and 5 IU hCG 48h apart. Mouse embryos were collected according to its developmental stage after the injection of hCG. Embryos were cryopreserved not only by cryoprotectant step (1 step${\sim}$4 step) but also in a variety of media (HTF, IVF medium, D-PBS) and cell stage. The results were as follows: There is no clear advantage in these freezing media of rapid method, but 4 cell and 8 cell of slow method (2, 3, 4 step) have advantage in D-PBS. The development of embryos according to cell stage become greater in 8 cell stage. In the treatment steps of cryopreservation, the development of embryo to blastocyst was similar among rapid method, but the development of 4 cell and 8 cell embryos to blastocyst according to slow method was better than rapid method.

• 한국도로학회논문집
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• 제18권1호
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• pp.63-72
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• 2016

PURPOSES : The use of roller-compacted concrete pavement (RCCP) is an environmentally friendly method of construction that utilizes the aggregate interlock effect by means of a hydration reaction and roller compacting, demonstrating a superb structural performance with a relatively small unit water content and unit cement content. However, even if an excellent structural performance was secured through a previous study, the verification research on the environmental load and long-term durability was conducted under unsatisfactory conditions. In order to secure longterm durability, the construction of an appropriate internal air-void structure is required. In this study, a method of improving the long-term durability of RCCP will be suggested by analyzing the internal air-void structure and relevant durability of roller-compacted concrete. METHODS : The method of improving the long-term durability involves measurements of the air content, air voids, and air-spacing factor in RCCP that experiences a change in terms of the kind of air-entraining agent and chemical admixture proportions. This test should be conducted on the basis of test criteria such as ASTM C 457, 672, and KS F 2456. RESULTS : Freezing, thawing, and scaling resistance tests of roller compacted concrete without a chemical admixture showed that it was weak. However, as a result of conducting air entraining (AE) with an AE agent, a large amount of air was distributed with a range of 2~3%, and an air void spacing factor ranging from 200 to $300{\mu}m$ (close to $250{\mu}m$) coming from PCA was secured. Accordingly, the freezing and thawing resistance was improved, with a relative dynamic elastic modulus of more than 80%, and the scaling resistance was improved under the appropriate AE agent content rate. CONCLUSIONS : The long-term durability of RCCP has a direct relationship with the air-void spacing factor, and it can be secured only by ensuring the air void spacing factor through air entraining with the inclusion of an AE agent.

• Journal of Animal Science and Technology
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• 제48권6호
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• pp.797-804
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• 2006

본 연구에서는 동결 보호제 EG l에 sucrose 첨가 농도에 따른 생존성의 실험의 결과를 요약하면 다음과 같다. 1.5 M EG와 1.8 M EG 만을 이용하여 동결융해 후 생존서의 조사한 결과 71.1%와 70.2%로 각각 나타났다. 총세포수에 있어서도 127±1.3개와 124±1.6개로 생존율과 총세포수에 있어서도 두 그룹간에는 유의적인 차이를 보이지 않았다. 1.5 M EG와 1.8 M EG에 0.1 M sucrose를 각각 첨가한 후 동결 보존하여 융해 하였을 때 생존율과 총세포수 조사 결과는 1.5 M EG에 0.1 M sucrose 처리구가 73.6% 그리고 1.8 M EG 에 0.1 M sucrose 첨가군은 76.9%의 결과를 보였으며 총세포수 에 있어서도 118±1.2 와 112±1.2 개의 결과를 보여 생존성과 총세포수에 있어서도 두 처리군 모두 유의적인 차이를 나타내지 않았으나 1.5 M EG 처리구에서 총세포수는 다소 높은 경향을 보였다. 1.5 M EG 와 1.8 M EG에 0.3 M sucrose를 첨가하여 각각 생존성과 총세포수 조사 결과는 70.8%와 88.7%의 생존율을 나타내어 1.8 M EG 에 0.3 M sucrose 처리구가 유의적으로(P<0.05) 높은 결과를 보였다. 따라서 소 체외수정란을 conventional slow-freezing 방법으로 동결 보존할 경우는 1.8 M EG 동결보호제에 0.3 M sucrose를 병행하여 사용하는 방법이 수정란을 최상의 상태로 유지할 수가 있어 수정란이식에 적용할 경우 효과적일 것으로 사료된다.

• 설비공학논문집
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• 제29권6호
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• pp.289-296
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• 2017

This study aimed to analyze the deck floor heating system of an outdoor swimming pool in terms of the thermal capacity/output and the surface temperature distribution based on the outdoor temperature, to design for anti-freezing during winter. Through the transient heat transfer simulation with PHYSIBEL and theoretical equations, the surface temperature distribution of the floor heating system at two outdoor conditions in Jeju, were calculated and evaluated. The results indicate that the specific thermal output required for maintaining $4^{\circ}C$ surface temperature at the design outdoor temperature of $0.1^{\circ}C$, was about $90W/m^2$. This performance analysis can be applied for future design criteria, including optimizations of system capacity and size.

• 한국식품위생안전성학회지
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• 제10권2호
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• pp.97-102
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• 1995

For improved preservation of boiled-dried anchovies, the current preservation method of corrugated-cardboard box packaging and freezing below -18$^{\circ}C$ was compared to the nylon/polyethylene(NY/PE) packaging along with cooling temperature ranging from 5$^{\circ}C$ to 1$0^{\circ}C$ as well as ambient condition for eight months by determining physicochemical quality of stored samples. Lipid oxidation of stored anchovies and their browning increased with storage time. The phenomenon was delayed under the conditions of lower temperature and air-tight packaging. As quality-indicative criteria of stored anchovies, carbonyl value(r=-0.989), browning (r=-0.949) and color a value(r=-0.989), browning(r=-0.965), Hunter's color b value(r=-0.949) and color a value(r=-0.940) showed a highly-negative correlations with organoleptic qualities of the samples. Based on the above results, air-tight packaging in a laminated film and subsequent storage at cooling temperature was found to be a possible alternative to the current freezing-storage of boiled-dried anchovies from the physicochemical point of view.

• Nuclear Engineering and Technology
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• 제12권2호
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• pp.88-98
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• 1980

원통형 관속을 흐르는 뜨거운 단상 유체의 응고 과정을 해석적인 방법과 실험적인 방법으로 연구 하였다. 파라핀초와 Wood's Metal을 뜨거운 유체로 사용하여 일련의 실험을 하였다. 이 실험 데이터로 부룩해븐 연구소에서 개발한 응고과정에 대한 기존 준정적 수리해석 모델을 증명하였다. 또한 이 실험결과, 수직관속을 순간적으로 흐르며 응고하는 과정에 미치는 여러가지 매개변수의 영향에 관한 자료를 얻게 되었다. 이 실험에 사용한 기구와 실험 방법 도 아울러 기술하였다. 녹은 유체의 순간적으로 흘러내리는 양에 대한 수학적 모델의 예측 결과를 실험데이터와 비교하기 위해 도표로 제시하였다. 또한, 수학적 모델을 고속증식로(LMFBR)에 사고가 일어 났을 경우에 응용하여 보았다.

• 한국도로학회논문집
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• 제20권3호
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• pp.11-18
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• 2018

PURPOSES : Durability of concrete is traditionally based on evaluating the effect of a single deterioration mechanism such as freezing & thawing action, chloride attack, carbonation and chemical attack. In reality, however, concrete structures are subjected to varying environmental exposure conditions which often results in multi-deterioration mechanism occurring. This study presents the experimental results on the durability of concrete incorporating air-cooled slag(AS) and/or water-cooled slag(WS) exposed to multi-deterioration environments of chloride attack and freezing & thawing action. METHODS : In order to evaluate durable performance of concretes exposed to single- and multi-deterioration, relative dynamic modulus of elasticity, mass ratio and compressive strength measurements were performed. RESULTS :It was observed that multi-deterioration severely affected durability of concrete compared with single deterioration irrespective of concrete types. Additionally, the replacement of cement by AS and WS showed a beneficial effect on enhancement of concrete durability. CONCLUSIONS : It is concluded that resistance to single- and/or multi-deterioration of concrete is highly dependent on the types of binder used in the concrete. Showing the a good resistance to multi-deterioration with concrete incorporating AS, it is also concluded that the AS possibly is an option for concrete materials, especially under severe environments.

• 조명전기설비학회논문지
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• 제27권2호
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• pp.95-101
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• 2013

This paper presents the comparison of the earth resistivity obtained from the measurements made with the three-electrode and four-electrode fall-of-potential techniques. The ${\rho}-a$ curve obtained from Wenner four-electrode method in undisturbed earth is in good agreement with the ${\rho}-l$ curve obtained from the three-electrode method based on the fall-of- potential method. However, The ${\rho}-a$ curve in disturbed earth with moisture and freezing is significantly different with the ${\rho}-l$ curve. The ${\rho}-a$ curve is considerably sensitive to the freezing and the moisture present in the earth surface compared to the ${\rho}-l$ curve. Thus to determine the actual earth resistivity, it is necessary to take into account the earth surface conditions when measuring the earth resistivity.