• Title/Summary/Keyword: freezing, thawing

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Geomorphological Processes of Jaein Waterfall and Dissection Valley in Hantangang Lava Plateau, Central Korea (한탄강 용암대지의 재인폭포와 개석곡의 지형 형성)

  • Lee, Min-Boo;Lee, Gwang-Ryul
    • Journal of The Geomorphological Association of Korea
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    • v.24 no.3
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    • pp.47-59
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    • 2017
  • This study aims to analyze geomorphologically Jaein Falls in Hantangang Lava Plateau for the landform structure, landform classification, falls dissection and recession. The height and recession length of the Falls are approximately 18m and 340m. after dissection valley arrive at area of the Jijangbong Volcanic Rock Complex of Mesozoic era, the fall will disappear. The structure of the dissection valley shows totally well vertical columnar joint near falls and plunge pool, colluvial talus deposits toward lower reach by the freezing-thawing and wet-dry activities, and alluvial cone and delta in estuary to Hantangang River. The Falls' age date and recession rate in the valley maybe relate to the age of the lava plateau. The estimated recession rate of the fall should be 8.75m/ka to 2.3m/ka, depending on the age 500ka to 40ka in lava plateau.

Crack-healing and durability performance of self-healing concrete with microbial admixture (미생물 혼입 자기치유 콘크리트의 균열 치유성능 및 내구성능)

  • Chu, Inyeop;Woo, Sang-Kyun;Lee, Byung-Jae;Lee, Yun;Lee, Hyo-Sub
    • KEPCO Journal on Electric Power and Energy
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    • v.7 no.2
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    • pp.295-299
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    • 2021
  • Recently, interest in maintenance has been increasing due to the enlargement and aging of infra structures. Therefore, a new paradigm is required to secure and improve the durability of structures differentiated from the past. Accordingly, research on smart concrete incorporating the concept of self-healing into concrete is being actively conducted. In this study, the crack healing performance and durability performance of self-healing concrete applied with a hydrogel containing biomineral-forming microorganisms were evaluated. As a result of evaluating the dispersion of the hydrogel in concrete, it was confirmed that the hydrogel was well distributed in concrete matrix with a dispersion coefficient of 0.35 to 0.46. The crack healing performance evaluation was verified by a water permeability test, and showed a recovery rate of 95% or more at the age of 28 days, confirming the applicability of self-healing concrete. The durability performance of self-healing concrete was evaluated in terms of resistance to penetration of chloride ion and freezing and thawing. Regardless of the mixing of the hydrogel, the same level of durability performance was shown for various compressive strength level. Therefore, it was confirmed that the microbial admixture did not affect concrete durability. In the future, long-term crack healing performance and durability verification studies should be supplemented.

Ecogeological Description of Sanyang Gotjawal, Jeju Island, Korea

  • Yi, Yun-Jae;Kim, Dae-Shin;Ko, Suk-Hyung;Lee, Keun Chul;Lee, Jung-Sook;Kim, Jong-Shik
    • Korean Journal of Environmental Agriculture
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    • v.41 no.3
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    • pp.217-221
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    • 2022
  • BACKGROUND: Gotjawal forest on Jeju Island is characterized by uneven topography that developed as a result of freezing-thawing weathering process and irregular substrates caused by numerous lava-flow collapses. Sanyang Gotjawal, located in southwest of Jeju Island, is a well-developed forest with a long history. In addition to photographs, there is a need for a good way to describe the unique features of Gotjawal, including its geology and vegetation. METHODS AND RESULTS: We illustrated the area's natural features using Clip Studio Paint 1.12.0. To reveal its complexity, we separated the vegetation and geological features in the illustrations. CONCLUSION(S): We drew a cross-section of Gotjawal's unique layers, including lava flows. In addition to an eco-geological approach, we described the dominant vegetation and geological characteristics of the three forest layers (trees, shrubs, and herbs) in Gotjawal.

Assessing the long-term durability and degradation of rocks under freezing-thawing cycles

  • Seyed Zanyar Seyed Mousavi;Mohammad Rezaei
    • Geomechanics and Engineering
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    • v.34 no.1
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    • pp.51-67
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    • 2023
  • In this research, the degradation rate of physical properties of the Angouran pit bedrock (calc-schist) is first investigated under the specific numbers of freeze-thaw (F-T) cycles. Then, the durability of calc-schist specimens against the F-T cycle number (N) is examined considering the mechanical parameters, and using the decay function and half-time techniques. For this purpose, point load strength (IS(50)), second durability index (Id2), Brazilian tensile strength (BTS), and compressive (VP) and shear (VS) wave velocities of calc-schist specimens are measured after 0, 7, 15, 40, and 75 N. For comparing the degradation rate of mechanical properties of available rock types on the Angouran mine walls, these tests are also carried out on the limestone and amphibolite schist specimens beside the calc-schist. According to test results, the exponential regression models are developed between the mechanical parameters of rock specimen's and N variable. Also, the long-term durability of each rock type versus N is studied using the decay function and half-time techniques. Results indicated that the degradation rate differs for the above rock types in which amphibolite schist and calc-schist specimens have the highest and least resistance against the N, respectively. The obtained results from this study can play a key role in the optimal design of the mine's final walls.

Optimal mixing proportion of bottom-ash-based controlled low strength material for high fillability

  • Youngsu Lee;Taeyeon Kim;Bongjik Lee;Seongwon Hong
    • Geomechanics and Engineering
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    • v.38 no.6
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    • pp.541-551
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    • 2024
  • Bottom ash classifies as a hazardous industrial-waste material that adversely affects human health. This study proposes its mixing with controlled low strength materials (CLSM) as a probable recycling approach. To this end, experiments have been performed to investigate the applicability of bottom-ash-based CLSM that comprises eco-friendly soil binders, water, fly ash, and a combination of bottom ash and weathered granite soil. The physical and chemical properties of the weathered granite soil, bottom ash, fly ash, and soil binders are analyzed via laboratory tests, including X-ray diffraction and scanning electron microscopy. To determine an appropriate CLSM mixing proportion, the flowability test is first performed on three mixture types having three replacement ratios of fly ash each. Subsequently, compressive-strength tests are performed. Based on the results of these tests, four mixtures are selected for the freeze-and-thaw test to determine the appropriate mixing proportion. Finally, the ground model and soil-contamination tests are performed to examine the field applicability of the mixture. This study confirms that bottom-ash-based CLSM causes negligible soil contamination, and it satisfies the prescribed performance requirements and contamination standards in Korea.

The Effect of Modified Cryopreservation Method on Viability of Frozen-thawed Primordial Germ Cell on the Korean Native Chicken (Ogye) (한국재래닭 (오계) 원시생식세포에 있어 동결방법의 개선이 융해 후 생존율에 미치는 영향)

  • Kim, Hyun;Kim, Dong Hun;Han, Jae Yong;Choi, Sung Bok;Ko, Yeoung-Gyu;Do, Yoon Jung;Seong, Hwan-Hoo;Kim, Sung Woo
    • Journal of Animal Science and Technology
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    • v.55 no.5
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    • pp.427-434
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    • 2013
  • This study was conducted to establish methods for preserving chicken primordial germ cells (PGCs) for long-term storage in liquid nitrogen and for developmental engineering or preservation of species. The purpose of this study is to clarify the effects of fetal bovine serum (FBS) or chicken serum (CS) treatment on the viability of cryopreserved PGCs from Korean Native Chicken (Ogye). PGCs separated from a germinal gonad of an early embryo at day 5.5-6 (stage 28) were suspended in a freezing medium containing freezing and protective agents (dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol). The values from 0, 5, 10, and 15 % DMSO plus FBS treatment were 21.6, 30.36, 36.42, 50.39, and 48.36 %, respectively. The viability of PGCs after freeze-thawing was significantly higher for 10% EG plus FBS treatment than for 10% EG + FCS treatment (p<0.05) (64.36% vs. 50.66%). This study establishes a method for preserving chicken PGC that enables systematic storage and labeling of cryopreserved PGC in liquid nitrogen at a germplasm repository and an ease of entry into a database. In the future, the importance for this new technology is that poultry lines can be conserved while work is being conducted to improve the production of germline chimeras.

Expression of EGFP in Bovine Embryos after Intracytoplasmic Sperm Injection using Spermatozoa Co-cultured with Exogenous DNA (소 난자에 있어서 외래유전자가 도입된 정자의 직접 주입에 의한 EGFP 의 발현)

  • Lee, . H. C.;S. J. Uhm;S. Y. Ann;H. J. Chung;Park, H. D.;Lee, H. T.;K. S. Chung
    • Korean Journal of Animal Reproduction
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    • v.24 no.4
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    • pp.439-449
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    • 2000
  • This study was to investigate the expression of transgene after co-injection of spermatozoon and EGFP gene into mature oocytes in cattle. From frozen semen, spermatozoa were treated by DTT with 0.03% Tween-20, freezing and thawing or 0.02% Triton X-100 to disrupt their plasma membranes. The sperm injected oocytes were co-cultured with bovine oviduct epithelial cells in CRlaa, and expression of EGFP in embryos were observbed under epifluorescent microscope. Two pronuclei were formed in oocytes injected with sperm treated by DTT (44.6%), DTT-Tween-20 (48.4%), DTT-freezing (44.4%) and DTT-Triton X-100 (42.9%). Cleavage and blastocyst formation rates of bovine oocytes which injected with sperm treated by DTT, DTT-Tween-20, DTT-freezing, and DTT-Triton X-100 were 49.1, 58.5, 43.9, and 48.4% and 10.2, 13.0, 6.8, and 6.5%, respectively. Although the most of embryos were showing mosaicism, embryos expressing EGFP gene were observed in all treated groups. Therefore, these results indicate that membrane disrupted sperm could interact with exogenous DNA, and that this procedure may be useful to introduce foreign gene into bovine oocytes.

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Type and Characteristics of Debris Landform in Mt. Mudeung (무등산 암설지형의 유형과 특징)

  • Oh, Jong-Joo;Park, Seoung-Phill;Seong, Yeong-Bae
    • Journal of the Korean association of regional geographers
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    • v.18 no.3
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    • pp.253-267
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    • 2012
  • The study looked into the type and characteristics of debris landforms in Mt. Mudeung. By focusing on the representative area, we aimed to categorize the debris landforms based on the morphologic and genetic characteristcis. The types of debris areas in Mt. Mudeung can be divided into the exposed debris type, mixed type of matrix, and the boulder-hidden type. Supply of block in the debris slope area displays different features depending on types of rocks. For the stony slopes of andesite, the block must be moved from the columnar joint or cliff in the upper part. The andesite debris slopes display dominant edge shape while displaying no round shape. The granite stony slopes display dominant round shape and the present exposed slope was assumed to be formed as the core stone which was deep weathered moved along slope during the periglacial era and the matrix was removed after post-glacial era. The movements of blocks are assumed to be caused by solifluction process. The joint area where granite and andesite areas meet, granite is located beneath andesite area, and this implies that blocks were actively freezing and creeping by solifluction and freezing and thawing at that time. It can be assumes that the granite matrix formed plain slope and then andesite boulder covered up the slope. Currently, the blocks in the stony slopes of Mt. Mudeung shows almost no mobility and the stony slopes created under periglacial climate can be considered to be fossil landform.

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Analysis on Adequacy of the Satellite Soil Moisture Data (AMSR2, ASCAT, and ESACCI) in Korean Peninsula: With Classification of Freezing and Melting Periods (인공위성 기반 토양 수분 자료들(AMSR2, ASCAT, and ESACCI)의 한반도 적절성 분석: 동결과 융해 기간을 구분하여)

  • Baik, Jongjin;Cho, Seongkeun;Lee, Seulchan;Choi, Minha
    • Korean Journal of Remote Sensing
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    • v.35 no.5_1
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    • pp.625-636
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    • 2019
  • Soil moisture is a representative factor that plays a key role in hydrological cycle. It is involved in the interaction between atmosphere and land surface, and is used in fields such as agriculture and water resources. Advanced Microwave Scanning Radiometer 2 (AMSR2), Advanced SCATterometer (ASCAT), and European Space Agency Climate Change Initiative (ESACCI) data were used to analyze the applicability and uncertainty of satellite soil moisture product in the Korean peninsula. Cumulative distribution function (CDF) matching and triple collocation (TC) analysis were carried out to investigate uncertainty and correction of satellite soil moisture data. Comparisons of pre-calibration satellite soil moisture data with the Automated Agriculture Observing System (AAOS) indicated that ESACCI and ASCAT data reflect the trend of AAOS well. On the other hand, AMSR2 satellite data showed overestimated values during the freezing period. Correction of satellite soil moisture data using CDF matching improved the error and correlation compared to those before correction. Finally, uncertainty analysis of soil moisture was carried out using TC method. Clearly, the uncertainty of the satellite soil moisture, corrected by CDF matching, was diminished in both freezing and thawing periods. Overall, it is expected that using ASCAT and ESACCI rather than AMSR2 soil moisture data will give more accurate soil moisture information when correction is performed on the Korean peninsula.

Development of Bovine Embryos after Vitrified-Thawed with Electron Microscope Grid and Open Pulled Straws

  • Lee, Y. J.;D.H. Ko;Lee, H. T.;K. S. Chung
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.76-76
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    • 2001
  • The objective of this study was to optimize the vitrification method of in vitro produced bovine embryos. Thus, in vitro produced embryos at 8 cell, morula and blastocyst stages were vitrified on electron microscope grids (EM grids) or in open pulled straws (OPS) with EG5.5 (5.5 M ethylene glycol, 1.0 M sucrose and 10% FBS in m-DPBS medium) freezing solution and their survival rates after thawing were compared. The embryos on EM grids or in OPS were briefly exposed to EG5.5 freezing solution and plunged directly into liquid nitrogen within 30 to 35 sec. Post-thawed embryos were serially diluted in 0.5, 0.25 and 0.125 M sucrose in m-DPBS, each for 1 min, and then cultured in CRI aa medium supplemented with 10% FBS. Embryonic survival rate was assessed as re-expanded and hatched rates of those embryos after warming. The rates of re-expansion embryos did not significantly different between EM grid (8 cell: 42.10%, morula: 66.66% and blastocyst: 77.08%) and OPS (8 cell: 47.36%, morula: 61.90% and blastocyst: 83.33%) methods. In addition, the hatched rates in EM grid (8 cell: 31.57%, morula: 57.14% and blastocyst: 72.91%) were similar to those in OPS (8 cell. 34.21%, morula: 50.00% and Blastocyst: 77.08%). Interestingly, even at the same blastocyst stage, the in vitro survival of day 7 embryos (EM grid: 79.48 and OPS: 87.18%) was higher than those of day 8 embryos (EM grid: 72.10 and OPS: 82.06%). The total cell number of blastocyst developed in vitro after vitrification was examined with Hoechst 33342 staining to compare the embryo quality among different treatment groups. The total cell number of blastocyst was not significantly different between vitrified groups (EM grid: 162.4$\pm$8.0 and OPS: 158.4$\pm$7.1) and unvitrified control (168.0$\pm$5.6). These results indicate that both vitrification containers can provide the high rate of embryo survival. Moreover, the OPS container may not need a cap to protect the container from floating after immersion in L$N_2$. Therefore, this study suggest that bovine embryos can be cryopreserved easily, effectively and successfully by vitrification method using EM grid or OPS with EG5.5 freezing solution. In the future, the Pregnancy rate would be investigated after transfer of our vitrified embryos into the appropriated recipients.

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