• 한국식품과학회지
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• 제22권5호
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• pp.514-519
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• 1990

양송이버섯의 신선도 연장을 위한 전리에너지 처리가 버섯의 품질에 관련된 휘발성 향기성분과 아미노산에 미치는 영향을 검토하였다. 시료의 주요 휘발성 성분으로 확인된 1-octen-3-ol(68%), benzaldehyde(13%), 3-octanone(8%), benzyl alcohol(5%), 3-octanol(2%), 1-octen-3-one(1%) 등의 eight-carbon 화합물과 방향족 화합물들을 2kGy의 감마선 조사와 저장조건(17일간, $9{\pm}1^{\circ}C,\;80{\pm}7%\;RH)$에 의해 함량의 증감현상이 나타났으나 대조시료와 감마선 조사시료에서 각각 추출된 휘발성 성분의 GC pattern에는 큰 차이를 보이지 않았다. 함유황유리아미노산은 2kGy의 감마선 조사에 의해 유의적으로 감소되었고, 그 밖의 대부분의 아미노산은 안정한 것으로 나타났다.

• Bulletin of the Korean Chemical Society
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• 제32권3호
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• pp.857-862
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• 2011

Kinetic studies of the reactions of N-methyl-Y-${\alpha}$-bromoacetanilides with substituted X-benzylamines have been carried out in dimethyl sulfoxide at $25.0^{\circ}C$. The Hammett plots for substituent X variations in the nucleophiles (log $k_N$ vs ${\sigma}_X$) are slightly biphasic concave upwards/downwards, while the Bronsted plots (log $k_N$ vs $pK_a$) are biphasic concave downwards with breakpoints at X = H. The Hammett plots for substituent Y variations in the substrates (log $k_N$ vs ${\sigma}_Y$) are biphasic concave upwards/downwards with breakpoints at Y = H. The cross-interaction constant $\rho_{XY}$ values are all negative: $\rho_{XY}$ = -0.32 for X = Y = electron-donating; -0.22 for X = electron-withdrawing and Y = electron-donating; -1.80 for X = electron-donating and Y = electronwithdrawing; -1.43 for X = Y = electron-withdrawing substituents. Deuterated kinetic isotope effects are primary normal ($k_H/k_D$ > 1) for Y = electron-donating, while secondary inverse ($k_H/k_D$ < 1) for Y = electronwithdrawing substituent. The proposed mechanisms of the benzylaminolyses of N-methyl-Y-${\alpha}$-bromoacetanilides are a concerted mechanism with a five membered ring TS involving hydrogen bonding between hydrogen (deuterium) atom in N-H(D) and oxygen atom in C = O for Y = electron-donating, while a concerted mechanism with an enolate-like TS in which the nucleophile attacks the ${\alpha}$-carbon for Y = electronwithdrawing substituents.

• The Korean Journal of Ecology
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• 제18권1호
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• pp.1-15
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• 1995

Alakline phosphatase activity (AP A) as a phosphorus deficiency measurement in flowing waters and of microhabitats (rocks, wood, leaves, and sediments) was measured and its relationship to flux of nutrients and response to rainfall events were determined for two geologically different streams in west Alabama from August to November. Results indicated water column AP A in both streams had a low correlation with levels of orthophosphate, total organic phosphorus, nitrate, ammonia, dissolved organic carbon, and discharge (r=0.075-0.583; n=g-IU. Communities on rock surfaces showed a higher AP A level than those on wood and leaves. Sediment passed through a $106{\mu}m$ sieve showed 2-9 times higher AP A level than material passed through $425{\mu}m$ sieve. The first storm after drought at Yellow Creek introduced substantial quantities of DOC (2.5 times baseflow concentrations) and $N0_3-N$ (5.8 times baseflow concentrations) which did not affect AP A significantly. The second storm at Little Schultz Creek caused minor changes in nutrient cocentrations; however $N0_3-N$ levels and AP A were drastically lower due to the dilution effect. Retention of stream water AP A at Yellow Creek and Little Schultz Creek on $0.45{\mu}m$ filter (54 and 43%, respectively) and $0.22{\mu}m$ (83 and 77% of total APA. respectively) indicated more free dissolved portion of the enzyme was present at Little Schultz Creek. Little Schultz Creek (with carbonate and with a higher productivity and biomass) showed a consistantly greater AP A activity $(132{\pm}54\;{\mu}M{\cdot}1^{-1}{\cdot}min^{-I};\;n=g)$ than Yellow Creek $(41{\pm}23\;{\mu}M{\cdot}1^{-I}{\cdot}min^{-I}$, with a sandstone substrate; n=l1, $p{\leq}O.OO1)$. Overall, a greater APA on all microhabitats and the presence of more dissolved enzyme in Little Schultz Creek during the study period may indicates it is more P deficient than Yellow Creek.

• Bulletin of the Korean Chemical Society
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• 제33권12호
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• pp.3943-3949
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• 2012

A series of aromatic hydrazides 3a-j were prepared by refluxing esters 2a-j with hydrazine hydrate in methanol, which were prepared by the esterification of 1a-j. Acetohydrazides 3a-j upon treatment with carbon disulfide and methanolic potassium hydroxide yielded potassium dithiocarbazate salts 4a-j, which on refluxing with hydrazine hydrate yielded substituted 4-amino-5-aryl-3H-1,2,4-triazole-3-thiones 5a-j. The target compounds 6a-j were synthesized by condensing furan-3-carboxylic acid in the presence of polyphosphoric acid under reflux. The structures of newly synthesized compounds were characterized by IR, $^1H$ NMR, $^{13}C$ NMR, elemental analysis and mass spectrometric studies. All the synthesized compounds were screened for their urease, acetylcholine esterase inhibition, antioxidant and alkaline phosphatase inhibition activity. Almost all of the compounds 6a-j showed good to excellent activities against urease and acetylcholine esterase more than the reference drugs. Compounds 6f and 6g were more potent scavenger of free radicals than the reference n-propyl gallate. Compound 6b and 6h showed excellent activities of alkaline phosphatase as compare to the reference $KH_2PO_4$.

• 대한치과보철학회지
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• 제41권3호
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• pp.342-350
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• 2003

Statement of problem. Titanium-Nickel alloy might be used in various prosthetic restorations since it has a unique property such as super-elasticity and high fatigue resistance. However, little is known about the casting ability of this alloy. Purpose. This in vitro study compared the casting accuracy and the porosity made with different investments and various sprue designs to ascertain what casting condition would be better for the fabrication of Ti-Ni cast restorations. Material and methods. A total of 70 Ti-Ni alloy crowns were made and divided into 7 groups of 10 copings on a metal master die. For measuring the effect of the sprue numbers, two groups with one and two 8-gauge sprues were compared. Moreover, the results of the conventional sprue and the double thickness sprues were compared. Three investments were used; carbon free phosphate bonded investment, titanium investment and gypsum bonded investment. The cast restorations were evaluated at 48 points on the entire circumferential margin with a stereomicroscope measuring in micrometers. Each crown was radiographically examined for casting defects and porosity. Data on casting accuracy were analyzed using two-way and Post hoc Scheffe's comparison to determine whether significant differences existed at the 95% confidence level. Student-Newman-Keuls test were performed to identify significant differences in the number of voids. Results. The double sprueing group and double thickness group had significantly less marginal discrepancy than the single sprueing group (P<.05 and P<.01, respectively). The castings with phosphate bonded investment showed the least marginal discrepancy and the smoothest surface. The castings invested in the gypsum bonded investment had the greatest gaps in margin and the largest failure rate. The double sprueing group and phosphate bonded investment group had significantly smaller void numbers and smaller void size than the other groups. Conclusion. Within the limitations of this in vitro study, the casting accuracy of the groups using thicker, double sprue design and the phosphate bonded investment was significantly superior. Moreover, void number and size were less than other groups.

• 한국재료학회지
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• 제5권6호
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• pp.657-666
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• 1995

Cu(hfac)$_2$,(Cu(II) hexafluoroacetylacetonate)를 프리커서로 하는 구리 화학증착에 대해 자유에너지 최소화법으로 열역학적 평형조성 계산을 수행하였다. Cu(hfac)$_2$-Ar계의 경우Cu(hfac)$_2$ 프리커서 자체의 열분해로부터 모든 공정조건에서 증착박막내로의 탄소 출입이 관찰되었다. Cu(hfac)$_2$-H$_2$,계에서는 Cu(hfac)$_2$-Ar계보다 낮은 온도에서 구리박막이 증착되며, H$_2$입력비 및 반응온도의 증가에 따라 응축상의 석출형태는 C(s)+CuF(s)로부터 C(s)+CuF(s)+Cu(s), C(s)+Cu(s), Cu(s), C(s)의 순으로 변화되는 것으로 나타났다.

• Korean Chemical Engineering Research
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• 제56권2호
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• pp.275-280
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• 2018

Methyl ester derived from coconut oil is very interesting to study since it contains free-fatty acid with chemical structure of medium carbon chain ($C_{12}-C_{14}$), so the methyl ester obtained from its part can be a biodiesel and another partially into biokerosene. The use of heterogeneous catalysts in the production of methyl ester requires severe conditions (high pressure and high temperature), while at low temperature and atmospheric conditions, yield of methyl ester is relatively very low. By using microwave irradiation trans-esterification reaction with heterogeneous catalysts, it is expected to be much faster and can give higher yields. Therefore, we studied the production of methyl ester from coconut oil using CaO catalyst assisted by microwave. Our aim was to find a kinetic model of methyl ester production through a transesterification process from coconut oil assisted by microwave using heterogeneous CaO catalyst. The experimental apparatus consisted of a batch reactor placed in a microwave oven equipped with a condenser, stirrer and temperature controllers. Batch process was conducted at atmospheric pressure with a variation of CaO catalyst concentration (0.5; 1.0; 1.5; 2.0, 2.5%) and microwave power (100, 264 and 400 W). In general, the production process of methyl esters by heterogeneous catalyst will obtain three layers, wherein the first layer is the product of methyl ester, the second layer is glycerol and the third layer is the catalyst. The experimental results show that the yield of methyl ester increases along with the increase of microwave power, catalyst concentration and reaction time. Kinetic model of methyl ester production can be represented by the following equation: $-r_{TG}=1.7{\cdot}10^6{_e}{\frac{-43.86}{RT}}C_{TG}$.

• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.199-206
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• 2012

Naturally immobilized tannase (tannin acyl hydrolase, E.C. 3.1.1.20) has many advantages, as it avoids the expensive and laborious operation of isolation, purification, and immobilization, plus it is highly stable in adverse pH and temperature. However, in the case of cell-associated enzymes, since the enzyme is associated with the biomass, separation of the pure biomass is necessary. However, tannic acid, a known inducer of tannase, forms insoluble complexes with media proteins, making it difficult to separate pure biomass. Therefore, this study optimizes the production of cell-associated tannase using a "protein-tannin complex" free media. An exploratory study was first conducted in shake-flasks to select the inducer, carbon source, and nitrogen sources. As a result it was found that gallic acid induces tannase synthesis, a tryptose broth gives higher biomass, and lactose supplementation is beneficial. The medium was then optimized using response surface methodology based on the full factorial central composite design in a 3 l bioreactor. A $2^3$ factorial design augmented by 7 axial points (${\alpha}$ = 1.682) and 2 replicates at the center point was implemented in 17 experiments. A mathematical model was also developed to show the effect of each medium component and their interactions on the production of cell-associated tannase. The validity of the proposed model was verified, and the optimized medium was shown to produce maximum cell-associated tannase activity of 9.65 U/l, which is 93.8% higher than the activity in the basal medium, after 12 h at pH 5.0, $30^{\circ}C$. The optimum medium consists of 38 g/l lactose, 50 g/l tryptose, and 2.8 g/l gallic acid.

• 분석과학
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• 제13권1호
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• pp.49-54
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• 2000

5단계 반응을 통하여 실리카겔의 8-hydroxyquinoline 유도체를 제조하였으며, 각 단계에서 얻은 생성물들을 IR과 NMR을 이용하여 분석하였다. 실리카겔 자체에 관한 IR 스펙트럼으로부터, 자유 hydroxyl기와 수소 결합된 hydroxyl기가 존재하는 것을 확인할 수 있었다. 첫 단계 반응에서는 N-H와 C-H의 IR 밴드와 APTS기에 있는 메틸렌 탄소의 NMR peak을 확인하였다. 둘째 단계에서는 카보닐, nitro, 방향족 탄소의 IR 밴드와 지방족, 방향족, 카보닐 탄소의 NMR peak을 관찰하였으며, 셋째 단계에서 $NO_2$가 $NH_2$로 환원되는 것을 IR과 NMR로 확인할 수 있었다. 마지막 단계에서는 4번째 단계에서 생성된 $N{\equiv}N$ IR peak이 사라지는 것으로부터 8-quinolinol이 고정화되는 것을 확인할 수 있었다.

• 한국표면공학회지
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• 제49권3호
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• pp.286-300
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• 2016

Pd-Ag alloy membranes have attracted a great deal of attention for their use in hydrogen purification and separation due to their high theoretical permeability, infinite selectivity and chemical compatibility with hydro-carbon containing gas streams. For commercial application, Pd-based membranes for hydrogen purification and separation need not only a high perm-selectivity but also a stable long-term durability. However, it has been difficult to fabricate thin, dense Pd-Ag alloy membranes on a porous stainless steel metal support with surface pores free and a stable diffusion barrier for preventing metallic diffusion from the porous stainless steel support. In this study, thin Pd-Ag alloy membranes were prepared by advanced Pd/Ag/Pd/Ag/Pd multi-layer sputter deposition on the modified porous stainless steel support using rough polishing/$ZrO_2$ powder filling and micro-polishing surface treatment, and following Ag up-filling heat treatment. Because the modified Pd-Ag alloy membranes using rough polishing/$ZrO_2$ powder filling method demonstrate high hydrogen permeability as well as diffusion barrier efficiency, it leads to the performance improvement in hydrogen perm-selectivity. Our membranes, therefore, are expected to be applicable to industrial fields for hydrogen purification and separation owing to enhanced functionality, durability and metal support/Pd alloy film integration.