• BMB Reports
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• v.37 no.5
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• pp.538-545
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• 2004

A new CRT binding factor (CBF) gene designated Cbcbf25 was cloned from Capsella bursa-pastoris, a wild grass, by the rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbcbf25 was 898 bp with a 669 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 223 amino acids. The predicted CbCBF25 protein contained a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and a possible acidic activation domain in the C-terminal region. Bioinformatic analysis revealed that Cbcbf25 has a high level of similarity with other CBF genes like cbf1, cbf2, and cbf3 from Arabidopsis thaliana, and Bncbf5, Bncbf7, Bncbf16, and Bncbf17 from Brassica napus. A cold acclimation assay showed that Cbcbf25 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. Our study implies that Cbcbf25 is an analogue of other CBF genes and may participate in cold-response, by for example, controlling the expression of cold-regulated genes or increasing the freezing tolerance of plants.

• The Journal of the Acoustical Society of Korea
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• v.19 no.3
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• pp.35-44
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• 2000

In this paper, we propose a new trajectory model for characterizing segmental features and their interaction based upon a general framework of hidden Markov models. Each segment, a sequence of vectors, is represented by a trajectory of observed sequences. This trajectory is obtained by applying a new design matrix which includes transitional information on contiguous frames, and is characterized as a polynomial regression function. To apply the trajectory to the segmental HMM, the frame features are replaced with the trajectory of a given segment. We also propose the likelihood of a given segment and the estimation of trajectory parameters. The obervation probability of a given segment is represented as the relation between the segment likelihood and the estimation error of the trajectories. The estimation error of a trajectory is considered as the weight of the likelihood of a given segment in a state. This weight represents the probability of how well the corresponding trajectory characterize the segment. The proposed model can be regarded as a generalization of a conventional HMM and a parametric trajectory model. The experimental results are reported on the TIMIT corpus and performance is show to improve significantly over that of the conventional HMM.

• Korean Journal of Microbiology
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• v.48 no.4
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• pp.293-297
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• 2012

Of the 169 strains of microorganisms stored in Polar and Alpine Microbial Collection of Korea Polar Research Institute, 27 strains were selected for their chitinase activity on ZoBell plates supplemented with 0.4% colloidal chitin. Among them, PAMC 21693 strain have shown the highest chitinolytic enzyme activity toward pNP-$(GlcNAc)_1$ at low temperature and the highest growth rate at $4^{\circ}C$. We cloned a full-length chitinase gene of 2,857 bp which contains an open reading frame of 2,169 bp encoding 872-amino acid polypeptide. Recombinant chitinase protein was expressed in E. coli and its molecular weight was confirmed 96 kDa. In this paper, we suggest the potential use of cold-active chitinase from polar microorganisms in the field of biotechnology.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.2A
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• pp.85-95
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• 2012

This paper presents an investigation on the ultimate behavior of steel cable-stayed bridges using nonlinear finite element analysis method. Cable-stayed bridges exhibit various geometric nonlinearities as well as material nonlinearities, so rational nonlinear finite element analysis should be performed for investigation of the ultimate behavior. In this study, ultimate behavior of steel cable-stayed bridges was studied using rational ultimate analysis method. Nonlinear equivalent truss element and nonlinear frame element were used for modeling the cable, girder and mast. Moreover, refined plastic hinge method was adopted for considering the material nonlinearity of steel members. In this study, the 2-step analysis method was used. Before live load analysis, initial shape analysis was performed in order to consider the dead load condition. For investigation of the ultimate behavior of steel cable-stayed bridges, analysis models which span length is 920.0 m were used. Radiating type and fan type were considered as the cable-arrangement types. With various quantitative evidences such as load-displacement curves, deformed shapes, locations of the yield point or region, bending moment distribution and so on, the ultimate behavior of steel cable-stayed bridges was investigated and described in this paper.

• Fisheries and Aquatic Sciences
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• v.17 no.3
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• pp.325-337
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• 2014

To assess the functional structure of prepro-melanin-concentrating hormone (pMCH), we isolated and cloned pMCH (of-pMCH) mRNA from the brain of the olive flounder, Paralichthys olivaceus, and compared its amino acid sequence with those from other animals. In addition, to examine whether activation of the brain of-pMCH gene is influenced by background color, density, and feeding, we compared pMCH mRNA activities against different background colors (bright and dark) and at different densities (100% PCA and 200% PCA). To examine whether the pMCH gene is related with malpigmentation of blind-side skin and appetite, we compared pMCH gene expression between ordinary and hypermelanic flounders, and between feeding and fasting flounders. The of-pMCH cDNA was 405 bp in the open reading frame [ORF] and encoded a protein of 135 amino acids; MCH was 51 bp in length and encoded a protein of 17 amino acids. An obvious single band of the expected size was obtained from the brain and pituitary by RT-PCR. In addition, of-pMCH gene activity was significantly higher in the bright background only at low density (< 100% PCA) making the ocular skin of fish whitening, and in ordinary fish. However, the gene activity was significantly decreased in dark background, at high density (>200% PCA), and in hypermelano fish. These results suggest that skin whitening camouflage of the flounder is induced by high MCH gene activity, and the density disturbs the function of background color in the physiological color change. Moreover, our data suggest that a low level of MCH gene activity may be related to malpigmentation of the blind-side skin. In feeding, although pMCH gene activity was significantly increased by feeding in the white background, the pMCH gene activity in the dark background was not influenced by feeding, indicating that the MCH gene activity increased by feeding can be offset by dark background color, or is unaffected by appetite. In conclusion, this study showed that MCH gene expression is related to ocular-skin whitening camouflage and blind-skin hypermelanosis, and is influenced by background color and density.

• Journal of Korean Society of Steel Construction
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• v.15 no.3
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• pp.231-239
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• 2003

Many box girder bridges have been constructed during the past several decades due to their large bending and torsional rigidities as well as aesthetic considerations. However, box girders have shortcoming in that the cross section distorts under an eccentric loading and warps out of the section plane. Therefore, in order to reduce distortional stresses such as distortional warping and transverse bending normal stresses, diaphragms were generally installed in the box girders. Shapes of the diaphragms in steel-box-girder bridges constructed up to date were solid-plate, frame, and truss types. The objectives of this study using parametric study were to evaluate the appropriate stiffness ratio of intermediate diaphragms and then to propose the effective spacing and numbers of intermediate diaphragms based on the evaluated stiffness ratio. Target bridges for this study were straight continuous span bridges with a single-cell steel box section. The parameters for the parametric study were the shape of box section, the span numbers, the equivalent span length, the stiffness of intermediate diaphragms, and the spacing of intermediate diaphragms. From the results of the parametric study, the effective spacing and numbers as well as the stiffness ratio of the intermediate diaphragms will be presented.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.748-755
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• 2013

This study was conducted to find a salt tolerance gene in Brassica rapa. In order to meet this objective, we analyzed data from a KBGP-24K oligo chip [BrEMD (Brassica rapa EST and microarray database)] of the B. rapa ssp. pekinensis 'Chiifu' under salt stress (250 mM NaCl). From the B. rapa KBGP-24K microarray chip analysis, 202 salt-responsive unigenes were primarily selected under salt stress. Of these, a gene with unknown function but known full-length sequence was chosen to closely investigate the gene function. The selected gene was named BrSSR (B. rapa salt stress resistance). BrSSR contains a 285 bp open reading frame encoding a putative 94-amino acid protein, and a DUF581 domain. The pSL94 vector was designed to over-express BrSSR, and was used to transform tobacco plants for salt tolerance analysis. T1 transgenic tobacco plants that over-expressed BrSSR were selected by PCR and DNA blot analyses. Quantitative real-time RT PCR revealed that the expression of BrSSR in transgenic tobacco plants increased by approximately 3.8-fold. Similar results were obtained by RNA blot analysis. Phenotypic characteristics analysis showed that transgenic tobacco plants with over-expressed BrSSR were more salt-tolerant than the wild type control under 250 mM NaCl for 5 days. Based on these results, we hypothesized that the over-expression of BrSSR may be closely related to the enhancement of salt tolerance.

• Journal of Life Science
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• v.24 no.12
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• pp.1291-1300
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• 2014

Peroxiredoxins (Prxs) are a ubiquitous family of antioxidant enzymes that participate in a variety of biological processes, including $H_2O_2$-mediated signal transduction, molecular chaperoning, and mitochondrial function. In this study, we isolated and characterized a Prx 2 cDNA from abalone (Haliotis discus hannai). The abalone Prx 2 cDNA encoded a 199-amino acid polypeptide that belongs to a class of typical 2-Cys Prxs that contain peroxidatic and resolving cysteines. The deduced abalone Prx 2 protein showed strong homology (64-99%) with Prx 2 proteins from other species, including mollusk, fish, amphibians, and mammals, and it was most closely related to disk abalone (H. discus discus) Prx 2. Abalone Prx 2 mRNA was ubiquitously detected in tested tissues, and its expression was comparatively high in the mantle, gills, liver, foot, and digestive duct. The expression level of abalone Prx 2 mRNA was 106.7-fold, 51.9-fold, and 437.8-fold higher, respectively, in the gills, digestive duct, and liver than in the muscles. The expression level of abalone Prx 2 mRNA in the liver peaked at 6 hr postinfection with Vibrio parahemolyticus and decreased at 12 hr postinfection. The expression level of abalone Prx 2 mRNA in hemocytes was drastically increased at 1 hr postinfection with V. parahemolyticus. These results suggest that abalone Prx 2 is conserved through evolution and that it may play a role similar to that of its mammalian counterpart.

• Journal of Korean Society of Steel Construction
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• v.21 no.6
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• pp.597-608
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• 2009

In this paper, large-deformation elasto-plastic analysis of space frames that considersjoint connection properties is presented. This method is based on the large-deformation formula with finite rotation, which was developed initially for elastic systems, and is extended herein to include the elasto-plastic effect and the member joint connection properties of semi-rigid what?. The analytical method was derived from the Eulerian concept, which takes into consideration the effects of large joint translations and rotations. The localmember force-deformation relationships were obtained from the beam-column approach, and the change caused by the axial strain in the member chord lengths and flexural bowing were taken into account. The effect of the axial force of the member on bending and torsional stiffness, and on the plastic moment capacity, is included in the analysis. The material is assumed to be ideally elasto-plastic, and yielding is considered concentrated at the member ends in the form of plastic hinges. The semi-rigid properties of the member joint connection are considered based on the power or linear model. The arc length method is usedto trace the post-buckling range of the elastic and elasto-plastic problems with the semi-rigid connection. A sample non-linear buckling analysis was carried out with the proposed space frame formulations to demonstrate the potential of the developed method in terms of its accuracy and efficiency.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.7 no.2
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• pp.156-162
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• 1995

A numerical method for a 2-D oil boom model considering the flexibility of skirt has been developed The neater is assumed rigid and the skirt is tensioned membrane having a point mass at its end The fluid motion is potential. The kinematic condition which demands the continuity of the displacement is imposed at the joint between the floater and the skirt. The dynamic condition for the point mass is imposed at the bottom end of the skirt. The numerical method is based on the Green's function method in the frame of linear potential theory. It finds it's solution simultaneously from the total system of three equations, integral equation, the equation of motion of the floater and the equilibrium equation of the deformation of the skirt. Integral equation is derived by applying the Green's theorem to radiation potential and Green's function. Proper descretization of those three equations leads to the system of a linear algebraic equation. Due to the flexibility of skirt the motion of floater can be diminished in some range of wave frequency and furthermore the mechanism of resonance of the oil boom can be changed. The motion responses of various oil booms have been compared varying the length of the skirt and the point mass. The numerical method has been validated indirectly from the good correspondence between the motion responses of the flexible skirt model and the rigid skirt model in low frequency limit.