• BMB Reports
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• v.46 no.7
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• pp.358-363
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• 2013

In this paper, we firstly reported a C-type lectin cDNA clone of 1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR and RACE techniques. The full-length cDNA contains an open reading frame encoding 308 amino acid residues which has two different carbohydrate-recognition domains (CRDs) arranged in tandem. To investigate the biological activities in the innate immunity, recombinant Ap-CTL was expressed in E. coli with a 6-histidine at the amino-terminus (Ap-rCTL). Besides acted as a broad-spectrum recognition protein binding to a wide range of PAMPs and microorganisms, Ap-rCTL also had the ability to recognize and trigger the agglutination of bacteria and fungi. In the proPO activation assay, Ap-rCTL specifically restored the PO activity of hemolymph blocked by anti-Ap-rCTL antibody in the presence of different PAMPs or microorganisms. In summary, Ap-rCTL plays an important role in insect innate immunity as an pattern recognition protein.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.8
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• pp.2555-2560
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• 1996

Inspecting the dimensional accuracy of a car-body in assembly line is a very important process to assure high productivity. Now there exist two common inspecting methods in practice. One is to measure a sampled car-body with three dimensional measuring machine, and the other is to measure car-body with three dimensional measuring machine, and the other is to measure car-body in assembly line using many sensors fixed to a large jig frame. The formal method takes too long to inspect a sampled car-body of a same sort, and cannot therefore give an useful error trend for the whole production. On the other hand, the latter lacks flexibility and is very cost-intensive. By using industrial robots and sensors, an in-line Car-Body Measuring(CBM) system which ensured high flexiblity and sufficient accuracy was developed. This CBM cell operates in real production line and measures the check points by the non-contact type using camera and laser displacement sensor(LDS). This system can handle about 15 Measuring points within a cycle time of 40 seconds. A process computer controls whole process such as data acquisition file handling and data analysis. Robot arms changes in length due to ambient temperature fluctuation affecting the measuring accuracy. To compensate this error, a robot arm calibration process was developed.

• Proceedings of the Botanical Society of Korea Conference
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• 1996.06a
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• pp.68-78
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• 1996

Two types of immunoloigcal probes, anti-ABBP Abs, have been developed. The purified ABBP from ABA-C1-BSA-sepharose 4B column was identified by PAGE and appeared in one band of about 56KD, as well as showed a specific binding ability and a high affinity for ABA (Kd2.0$\times$10-9 mol/L). Unexpectedly, the existence of rRNA with a length of around 300 nucleotides could be found, when the ABBP was digested with proteinase K and identified by eletrophorsis on an agarose gel (1%). As a result, about 120 cDNA clones coding maize 17s RNA and only one cDNA clone coding ABBP (24cDNA) were obtained from 200,000 seperated phage plaques by the anti-ABBP pAbs. 24cDNA had 1075bp and contained an open reading frame coding 254 amino acids. The anti-idiotypic Ab raised against an ABA MAb showed the ability of either mimicking ABA or competing with ABA. The localization of ABBPs in plant cell was investigated.

• International Journal of Industrial Entomology and Biomaterials
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• v.23 no.2
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• pp.223-229
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• 2011

Cyclophilins are originally identified as cytosolic binding protein of the immunosuppressive drug cyclosporine A. They have an activity of peptidyl prolyl cis/trans-isomerases (PPIase), which may play important roles in protein folding, trafficking, assembly and cell signaling. In this study, we report the cloning and characterization of a Bombyx mori cyclophilin A (bCypA) cDNA. The full-length cDNA of bCypA consist of 947 nucleotides with a polyadenylation signal sequence AATAAA and contain an open reading frame of 498 nucleotides encoding a polypeptide of 166 amino acids. The deduced amino acid sequence of bCypA shares a central peptidyl prolyl cis/trans-isomerase and a cyclosporin-A-binding domain with other cyclophilin sequences. Relative quantification real-time (RT) PCR analysis shows that mRNA transcripts of bCypA are detected in all the investigated tissues and highest expression level in the skin of 3-day-old 5 instar larva. Also, bCypA had PPIase activity on the proline-containing peptides. Accordingly, we suggest that bCypA is a new member of the cyclophilin A (CyPA) family and will be useful for quality control of bioactivity recombinant proteins with proline-containing peptides.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.37 no.3
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• pp.9-17
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• 2000

In this paper we implement computer simulation system of 4FSK signal MODEM using Quadrature detector and analyze overall tranceiver system. We follow the FLEX wireless paging system standards and construct premodulation filter and data frame. We propose an efficient open loop symbol timing recovery algorithm which takes advantage of 128 bit length preamble pattern and also propose a 32 bit UW pattern which Is based on the optimal UW detection method, and excellent aperiodic autocorrelation characteristic. The BER simulation in the fading channel as well as AWGN is performed with BCH coding and Interleaving to the Quadrature detector system and it is shown that a high coding fain occurs in the fading channel rather than AWGN channel.

• The Journal of the Acoustical Society of Korea
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• v.37 no.2
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• pp.110-117
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• 2018

In this paper, we propose a low delay window switching MDCT (Modified Discrete Cosine Transform) method for speech/audio coder. The window switching algorithm is used to reduce the degradation of sound quality in non-stationary trasient duration and to reduce the algorithm delay by using the low delay TDAC (Time Domain Aliasing Cancellation). While the conventional window switching algorithms uses overlap-add with different lengths, the proposed method uses the fixed overlap add length. It results the reduction of algorithm delay by half and 1 bit reduction in frame indication information by using 2 window types. We apply the proposed algorithm to G.729.1 based on MDCT in order to evaluate the performance. The propose method shows the reduction of algorithm delay by half while speech quality of the proposed method maintains same as the conventional method.

• Korean Journal of Optics and Photonics
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• v.17 no.2
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• pp.149-158
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• 2006

We have analyzed various tolerances of the multi-configurative microscopic system for inspecting the wire-bonding of a reed frame by using the Gaussian bracket method and the equivalent lens method. The tolerances for the curvature and the thickness, which are axial symmetric tolerances, are given by varying the back focal length within a fecal depth under diffraction-limited conditions. Moreover, by using the trial and error method, the axial non-symmetric tolerances for decenter and tilt are established by assigning the 5% variation of MTF(modulation transfer function) at the spatial frequency of 50 lp/mm and at the field angle of 0.7 field. As the tolerances with the most probable distribution are distributed within the range of the decay rate of less than 5% independent of the probability distribution of tolerances, we can achieve completely the desired design performances of the multi-configurative microscopic system by using the various ranges of these tolerances.

• Animal cells and systems
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• v.15 no.1
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• pp.29-36
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• 2011

Insect lysozymes are basic, cationic proteins synthesized in fat body and hemocytes in response to bacterial infections and depolymerize the bacterial cell wall. The c-type lysozyme of the insect Spodoptera litura (SLLyz) is a single polypeptide chain of 121 residues with four disulfide bridges and 17 rare codons and is approximately 15 kDa. The full-length SLLyz cDNA is 1039 bp long with a poly(A) tail, and contains an open reading frame of 426 bp long (including the termination codon), flanked by a 54 bp long 5' UTR and a 559 bp long 3' UTR. As a host for the production of high-level recombinant proteins, E. coli is used most commonly because of its low cost and short generation time. However, the soluble expression of heterologous proteins in E. coli is not trivial, especially for disulfide-bonded proteins. In order to prevent inclusion body formation, GST was selected as a fusion partner to enhance the solubility of recombinant protein, and fused to the amplified products encoding mature SLLyz. The expression vector pGEX-4T-1/rSLLyz was then transformed into E. coli BL21(DE3)pLysS for soluble expression of rSLLyz, and the soluble fusion protein was purified successfully. Inhibition zone assay demonstrated that rSLLyz showed antibacterial activity against B. megaterium. These results demonstrate that the GST fusion expression system in E. coli described in this study is efficient and inexpensive in producing a disulfide-bonded rSLLyz in soluble, active form, and suggest that the insect lysozyme is an interesting system for future structural and functional studies.

• Journal of the Korea institute for structural maintenance and inspection
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• v.15 no.1
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• pp.271-280
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• 2011

This study intends to analytically evaluate the vibration serviceability of the bridges for each long-span type having the same span length and road width using the Meister vibration sensation curve. With MIDAS, a structural analysis program, bridges were modeled using the girders as the frame element and slabs as the plate element. The transient analysis was performed using the moving loads of the design vehicles. This study presents the analytical process of reviewing the vibration serviceability during the design of long-span bridges. It involves the comparison of the vibration serviceability of different bridge types by applying the lagging-behind and acceleration amplitude from transient analysis to Meister curve. The result confirms that the process is appropriate.

• Environmental Analysis Health and Toxicology
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• v.26
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• pp.5.1-5.11
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• 2011

Objectives: In order to identify the possibility of slender bitterling (SB) (Acheilognathus yamatsutae) being used as a test species for estrogenic endocrine disrupting chemicals (EEDCs), we carried out the cloning and sequence characterization of the estrogen receptor (ER). Methods: The ER from a slender bitterling was obtained by reverse transcriptase-polymerase chain reaction (RT-PCR), 5'- and 3'-rapid amplification of cDNA ends (5'-RACE and 3'-RACE) and T-vector cloning. The expression of ER mRNA was also analyzed in six tissues (brain, liver, kidney, gill, gonad, and intestines) by real-time PCR. Results: We obtained an ER from the slender bitterling. The SB ER cDNA was 2189 base pairs (bp) in length and contained a 1707 bp open reading frame that encoded 568 amino acid residues. The SB ER amino acid sequence clustered in a monophyletic group with the $ER{\alpha}$ of other fish, and was more closely related to zebrafish $ER{\alpha}$(88% identity) than to the $ER{\alpha}$ of other fish. The SB ER cDNA was divided into A/B, C, D, E and F domains. The SB ER has conserved important sequences for ER functions, such as the DNA binding domain (D domain), which are consistent with those of other teleosts. Conclusions: The ER of the slender bitterling could provide basic information in toxicological studies of EEDCs in the slender bitterling.