• BMB Reports
• /
• v.39 no.5
• /
• pp.626-635
• /
• 2006

Lysophosphatidic acid acyltransferase (LPAAT) is an intrinsic membrane protein that catalyzes the synthesis of phosphatidic acid (PA) from lysophosphatidic acid (LPA). It is well known that LPAAT is involved in lipid biosynthesis, while its role in tumour progression has been of emerging interest in the last few years. To date, seven members of the LPAAT gene family have been found in human. Here we report a novel LPAAT member, designated as LPAAT-theta, which was 2728 base pairs in length and contained an open reading frame (ORF) encoding 434 amino acids. The LPAAT-theta gene consisted of 12 exons and 11 introns, and mapped to chromosome 4q21.23. LPAAT-theta was ubiquitously expressed in 18 human tissues by RT-PCR analysis. Subcellular localization of LPAAT-theta-EGFP fusion protein revealed that LPAAT-theta was distributed primarily in the endoplasmic reticulum (ER) of COS-7 cells. Furthermore, we found that the overexpression of LPAAT-theta can induce mTOR-dependent p70S6K phosphorylation on Thr389 and 4EBP1 phosphorylation on Ser65 in HEK293T cells.

• BMB Reports
• /
• v.40 no.3
• /
• pp.358-367
• /
• 2007

A novel mannose-binding tuber lectin with in vitro antiproliferative activity towards human cancer cell lines and antiviral activity against HSV-II was isolated from fresh tubers of a traditional Chinese medicinal herb, Typhonium divaricatum (L.) Decne by a combined procedure involving extraction, ammonium sulfate precipitation, ion exchange chromatography on DEAE-SEPHAROSE, CM-SEPHAROSE and gel-filtration on sephacryl S-200. The apparent molecular mass of the purified Typhonium divaricatum lectin (TDL) was 48 kDa. TDL exhibits hemagglutinating activity toward rabbit erythrocytes at 0.95 $\mu$g/ml, and its activity could be strongly inhibited by mannan, ovomucoid, asialofetuin and thyroglobulin. TDL showed antiproliferative activity towards some well established human cancer cell lines, e.g. Pro-01 (56.7 $\pm$ 6.8), Bre-04 (41.5 $\pm$ 4.8), and Lu-04 (11.4 $\pm$ 0.3). The anti-HSV-II activity of TDL was elucidated by testing its HSV-II infection inhibitory activity in Vero cells with $TC_50$ and $EC_50$ of 5.176 mg/ml and 3.054 $\mu$g/ml respectively. The full-length cDNA sequence of TDL was 1145 bp and contained an 813-bp open reading frame (ORF) encoding a 271 amino acid precursor of 29-kDa. Homology analysis showed that TDL had high homology with many other mannose-binding lectins. Secondary and three-dimensional structures analyses showed that TDL is heterotetramer and similar with lectins from mannose-binding lectin superfamily, especially those from family Araceae.

• Steel and Composite Structures
• /
• v.17 no.3
• /
• pp.215-236
• /
• 2014

The flexural behaviour of steel beams significantly affects the structural performance of the steel frame structures. In particular, the flexural overstrength (namely the ratio between the maximum bending moment and the plastic bending strength) that steel beams may experience is the key parameter affecting the seismic design of non-dissipative members in moment resisting frames. The aim of this study is to present a new formulation of flexural overstrength factor for steel beams by means of artificial neural network (NN). To achieve this purpose, a total of 141 experimental data samples from available literature have been collected in order to cover different cross-sectional typologies, namely I-H sections, rectangular and square hollow sections (RHS-SHS). Thus, two different data sets for I-H and RHS-SHS steel beams were formed. Nine critical prediction parameters were selected for the former while eight parameters were considered for the latter. These input variables used for the development of the prediction models are representative of the geometric properties of the sections, the mechanical properties of the material and the shear length of the steel beams. The prediction performance of the proposed NN model was also compared with the results obtained using an existing formulation derived from the gene expression modeling. The analysis of the results indicated that the proposed formulation provided a more reliable and accurate prediction capability of beam overstrength.

• Earthquakes and Structures
• /
• v.15 no.2
• /
• pp.193-202
• /
• 2018

This paper studied the probability of pounding occurred between decks and abutments of a long span high-pier continuous rigid fame bridge subjected to ground motions with local soil effect. A pounding probability analysis methodology has been proposed using peak acceleration at bedrock as intensity measure (IM) for multi-support seismic analysis. The bridge nonlinear finite element (FE) models was built with four different separation distances. Effect of actual site condition and non-uniform spatial soil profiles on seismic wave propagating from bedrock to ground surface is modelled. Pounding probability of the high-pier bridge under multi-support seismic excitations (MSSE) is analyzed based on the nonlinear incremental dynamic analysis (n-IDA). Pounding probability results under uniform excitations (UE) without actual local site effect are compared with that under MSSE with site effect. The study indicates that the required design separation length between deck and abutment under uniform excitations is larger than that under MSSE as the peak acceleration at bedrock increases. As the increase of both separation distance between deck and abutment and the peak acceleration, the probability of pounding occurred at a single abutment or at two abutments simultaneously under MSSE is less than that under UE. It is of great significance considering actual local site effect for determining the separation distance between deck and abutment through the probability pounding analysis of the high-pier bridge under MSSE.

• Journal of IKEEE
• /
• v.15 no.3
• /
• pp.198-204
• /
• 2011

In this letter, another analytical approach for the opportunistic amplify-and-forward (OAF) relay systems is proposed over Rayleigh fading channels. Based on set-operation at the selected relay node, its selection probability as the best relay is derived and then, the probability density function (PDF) of the received instantaneous signal-to-noise ratio (SNR) is expressed as a more tractable form in which the number of summations and the length of each summation are specified. Then, the average error rate, outage probability, and average channel capacity are obtained as approximated closed-forms. Simulation results are finally presented to validate that the proposed analytical expressions can be a unified frame work covering all Rayleigh fading channel conditions. Furthermore, it is confirmed that OAF schemes can outperform the other non-selective schemes on the average error rate, outage probability, and average channel capacity.

• Journal of the Korean Institute of Telematics and Electronics C
• /
• v.36C no.1
• /
• pp.1-11
• /
• 1999

In this paper, we proposed a design method of parameterized viterbi decoder, which automatically synthsizes the diverse viterbi deciders used in the digital mobile communication systems. It is designed to synthesize a viterbi decoder specified by user-provided parameters. Those parameters are constraint length, code rate generator polynomials of teh convolutional encoder, data rate and bits/frame of the data transmission, and soft decision bits of viterbi decoder. For the design of the parameterized viterbi decoder, we designed a user interface module C-language, and a viterbi decoder module in a hierarchical atructure using VHDL language and its generic statement. For the verification of the parameterized viterbi decoder, we compared our synthesized viterbi decoder with the conventional viterbi decoder which is designed for the IS-95 CDMA system. The proposed design method of the viterbi decoder will be a new method to obtain a required viterbi decoder in a very short time only by supplying the design parameters.

• Journal of the Institute of Electronics Engineers of Korea TC
• /
• v.44 no.10
• /
• pp.156-161
• /
• 2007

In a TDD system, the length of slots can be unequal, and the number of downlink (DL) and uplink (UL) slots per frame can be different as well. The advantage of using TDD is the capability to accommodate asymmetric high-bit-rate services for the DL and It, which will be one of the prominent features in 4G systems. This paper analyzes the performance of TDD system on mobile channel environments like indoor pedestrian and vehicular channel, and proposes optimum modulation/demodulation method in TDD system. A rectangular QAM (RQAM) used in various communication systems has good BER performance but the much more signal amplitudes also have become one of the barriers to implement receiver. While PSK receiver is implemented easily because it has a constant amplitude, but it's BER performance is worse than RQAM. APSK proposed in this paper integrates merits of RQAM and PSK, and minimizes demerits of then And a simple method is also proposed to demodulate the soft symbol. The results indicate that the proposed APSK has a little worse performance than RQAM but the dynamic range of APSK is about 4 dB, 8 dB better than RQAM at 16-ary, 64-ary modulation/demodulation respectively.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.18 no.2
• /
• pp.159-168
• /
• 2005

An improved stability design method for beam-columns of plane frames is proposed based on system buckling analysis and second-order elastic analysis. For this, the tangent stiffness matrix of beam-column elements is first derived using stability functions and a procedure for evaluating effective buckling lengths is reviewed using elastic system buckling analysis. And then the second-order analysis procedure is presented considering $P-\Delta$ effects and is compared with the closed-form solution through numerical examples. Design examples showing the validity of the proposed method we presented and their numerical results are compared with those obtained from the conventional stability design methods. Finally some useful conclusions are drawn.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.18 no.4 s.70
• /
• pp.395-404
• /
• 2005

This paper presents a reliable numerical procedure for nonlinear time-history analysis of space steel frames subjected to dynamic loads. Geometric nonlinearities of member (P-$\delta$) and frame (P-$\Delta$) are taken into account by the use of stability functions in framed stiffness matrix formulation. The gradual yielding along the member length and over the cross section is included by using a tangent modulus concept and a softening plastic hinge model based on the New-Orbison yield surface. A computer program utilizing the average acceleration method for the integration scheme is developed to numerically solve the equation of motion of framed structure formulated in an incremental form. The results of several numerical examples are compared with those derived from using beam element model of ABAQUS program to illustrate the accuracy and the computational efficiency of the proposed procedure.

• Korean Journal of Plant Resources
• /
• v.18 no.3
• /
• pp.374-381
• /
• 2005

A full-length cDNA encoding ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) has been isolated and its nucleotide sequence determined from root in ginseng plant (Panax ginseng). The rbcS cDNA of ginseng is 790 nucleotides long and has an open reading frame of 549 bp with deduced amino acid of 183 residues (pI 8.37), 20.5 kDa. The deduced amino acid sequence of rbcS matched to the previously reported rbcS protein genes and showed a high similarity with the $78\%$ identity with rbcS of Helianthus annuus (CAA68490). In the phylogenetic analysis based on the amino acid residues, the ginseng rbcS was clustered with H. annuus (CAA68490), C. morifolium (AA025119) and L. sativa (Q40250).