• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.399-405
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• 2001

Urushiol, a natural pro-electrophilic quinone compound, has potential structural characteristics as antitumor chemotherapeutic agents. However, urushiol's use as an antitumor drug has some problems, because it is hardly miscible with an aqueous solution. Purified urushiol is highly viscous and soluble only in strong solvents. for this study, we prepared an urushiol-ethanol micro-emulsion with a unimodal size distribution by high-speed homogenization. This generated effective delivery of urushiol to its action wites, so that we could investigate its cytotoxic activity against cancer cells. Using a colony-forming assay, we were able to show that urushiol selectively inhibited the growth of the ovarian cancer cells PA-1 and 2774 at a concentration of $10^{-6}$, whereas it had only a negligible effect on normal CHO cells at the same concentration. The data suggest that urushiol may have potential as an effective antitumor agent in the treatment of ovarian cancer. In addition, we addressed the question of whether the specific cytotoxic effect of urushiol is linked to apoptosis, by DNA fragmentation and DAPI staining assays. The inhibitory effects of urushiol on the growth of ovarian cancer cells was found to be associated with DNA fragmentation and the fragmented nuclei formation, both of which represent markers for the induction of apoptosis. Therefore, the results suggested that urushiol affected its profound cytotoxicity by triggering apoptosis in ovarian cancer cells.

• 한국환경생태학회지
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• 제22권2호
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• pp.128-137
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• 2008

본 연구는 북한산 국립공원을 대상으로 등산로가 산림경관의 파편화에 미치는 영향을 분석한 후, 이를 토대로 소유역별 경관생태학적 건전성을 평가하였다. 등산로 개설에 의한 영향 분석 결과에 따르면, 산림패치의 크기는 감소한 반면 패치 및 가장자리 밀도는 증가한 것으로 나타났다. 또한, 패치형태는 보다 복잡해지고, 핵심지역의 면적은 뚜렷하게 감소하였고, 패치간의 근접도는 증가하는 것으로 나타났다. 한편, 소유역별 경관생태학적 건전성 평가 결과, 종합적인 건전성은 북한산유역이 3.7점으로 가장 높게 나타났으며, 구기유역이 1.6점으로 가장 낮게 평가되었다. 이상의 결과를 종합해 볼 때, 산림의 경관생태학적 보전과 관리를 위해서는 불필요한 등산로의 개설을 금지하고, 훼손 및 교란 지역에 대한 자연적인 생태학적 복원이 이루어져야 할 것으로 판단된다.

• Bulletin of the Korean Chemical Society
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• 제23권2호
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• pp.309-314
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• 2002

The ablation dynamics and cluster formation of $C_n^+$ ions ejected from 355 nm laser ablation of a graphite target in vacuum are investigated using a reflectron time-of-flight (RTOF) mass spectrometer. At low laser fluence, odd-numbered cluster ions with $3{\leq}n{\leq}15$ are predominantly produced. Increasing the laser fluence shifts the maximum size distribution towards small cluster ions, implying the fragmentation of larger clusters within the hot plume. The temporal evolution of $C_n^+$ ions was measured by varying the delay time of the ion extraction pulse with respect to the laser irradiation, providing significant information on the characteristics of the ablated plume. Above a laser fluence of $0.2J/cm^2$ , large cluster ions ($n{\geq}30$) are produced at relatively long delay times, indicating that atoms or small carbon clusters aggregate during plume propagation. The dependence of the intensity of ablated $C_n^+$ ions on delay time after laser irradiation shows that the most probable velocity of each cluster ion decreases with cluster size.

• 한국조경학회지
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• 제35권5호
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• pp.56-63
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• 2007

This study used the nearest feature model to connect forest patches within the western Geum River Basin. Due to many different forest patch sizes, 3 alternative methods were tested to determine the best way to establish an ecological network with forest patches. Alternative 1 used all forest patches to determine whether patches were large enough. Alternative 2 used forest patches over 10 ha in size. Alternative 3 used natural conservation indices to select forest patches containing better qualities in the natural conservation level. As a result 635 out of 724 patches of over 10 ha were selected for comparison. Alternative 1 showed that forest patches of less than 10 ha were outliers interrupting the establishment of the ecological network. They generated an unnecessary ecological network to link core areas to comparison features. The ecological network was improved by using forest patches greater than 10 ha in size(Alternative 2). Each comparison feature was much more hierarchically connected to core areas in Alternative 2 than in Alternative 1. Forest patches filtered by natural conservation indices were useful for obtaining the best ecological network. Alternative 3 clearly showed the connections in the ecological network between core areas and forest.

• 한국수정란이식학회지
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• 제10권1호
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• pp.45-51
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• 1995

In order to determine the optimum condition and timing for in vitro maturation of oocytes to metaphase of meiosis II (M II), the immatured follicular oocytes were recovered by puncturing the large(1.0~1.5 mm in diameter) and small(<1.0 mm in diameter) follicles in the ovaries of rabbits treated intramuscularly with a single dose of 100 TU PMSG 68 hours previously. The follicular oocytes were classified into three grades by the attachment of cumulus cells. The Grade I and II follicular oocytes from large follicles were cultured in BO-DM medium with 10% FCS, 35 $\mu$g /nl of FSH, 10 $\mu$g /ml of LH and 1 $\mu$g /ml of estradiol-17$\beta$ at 39t in a 5% $CO_2$ incubator for 11 to 23 hours. In 3 hours interval during the culture period, the oocytes were harvested and their cumulus cells were removed with hyaluronidase. The denuded oocytes were stained with Hoechst 33342 dye and their meiotic status and extrusion of the first polar body (PB) were examined under a fluorescence microscope. Also the fragmentation of the first PB and the distance between the first PB and nucleus were examined. The results obtained were as follows: 1. The mean recovery rate of follicular oocytes from the large and small follicles was 59. 9 and 31.3%, respectively. The mean number of oocytes recovered per rabbit and the Grade I percentage were 14.6 and 94.4% in large follicles, but 2.1 and 61.1% in small follicles, respectively. All the parameters examined were different significantly (p<0.05) between both the folliclular size. 2. Most of the follicular oocytes(86.8%) were matured in vitro to M II phase in 14 hours in Grade I oocytes, but the significantly(p<0.05) less oocytes(45.5%) were matured in Grade II oocytes. 3. The first PB was extruded in most of the oocytes(94.7%) in 14 hours of culture with the fragmentation rate of 29.6%, but the fragmentation rate of the first PB increased significantly (p<0.05) as the culture period for maturation was longer to 20 hours(63.5%). 4. The distance between the first PB and nucleus was increased linearly (p<0.05) as the maturation time passed from 14(7.1$\mu$rn) to 23 hours(58.4$\mu$m). 5. From the above results it was concluded that the optimum time for in vitro maturation culture might be 14 hours in the follicular oocytes from rabbit primed with PMSG for 68 hours, expecially when these follicular oocytes were used for recipient cytoplasms in embryo cloning.

• BMB Reports
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• 제33권2호
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• pp.133-137
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• 2000

To elucidate the effect of oxygen radicals on the molecular properties of proteins, the secondary and tertiary structure and molecular weight size of BSA and ${\beta}$-lactoglobulin were examined after irradiation of proteins at various doses. Gamma-irradiation of protein solutions caused the disruption of the ordered structure of protein molecules as well as degradation, cross-linking, and aggregation of the polypeptide chains. As a model system, BSA and ${\beta}$-lactoglobulin were used as a typical ${\alpha}$-helical and a ${\beta}$-sheet structure protein, respectively. A circular dichroism study showed that the increase of radiation decreased the ordered structure of proteins with a concurrent increase of aperiodic structure content. Fluorescence spectroscopy indicated that irradiation quenched the emission intensity excited at 280 nm. SDS-PAGE and a gel permeation chromatography study indicated that radiation caused initial fragmentation of proteins resulting in a subsequent aggregation due to cross-linking of protein molecules.

• BMB Reports
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• 제35권6호
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• pp.590-594
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• 2002

To elucidate the effect of gamma-irradiation on the molecular properties of myoglobin, the secondary and tertiary structures, as well as the molecular weight size of the protein, were examined after irradiation at various irradiation doses. Gamma-irradiation of myoglobin solutions caused the disruption of the ordered structure of the protein molecules, as well as degradation, cross-linking, and aggregation of the polypeptide chains. A SDS-PAGE study indicated that irradiation caused initial fragmentation of the proteins and subsequent aggregation, due to cross-linking of the protein molecules. The effect of irradiation on the protein was more significant at lower protein concentrations. Ascorbic acid protected against the degradation and aggregation of proteins by scavenging oxygen radicals that are produced by irradiation. A circular dichroism study showed that an increase of the irradiation decreased the a-helical content of myoglobin with a concurrent increase of the aperiodic structure content. Fluorescence spectroscopy indicated that irradiation increased the emission intensity that was excited at 280 nm.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2004년도 추계학술대회 논문집
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• pp.94-97
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• 2004

Study of a micro chemical plant consisted with droplet generation, digital sorting and separation was conducted. The droplet generation was quantitatively characterized. The digital sorting brought effective reaction by shortening the droplet size and the pitch of droplet generation simultaneously. To prevent the unfavorable higher order reaction, perfect separation of droplets was visualized.

• Toxicological Research
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• 제21권4호
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• pp.303-307
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• 2005

Nanomaterials, which ranges in size from 1 to 100 nm, have been used to create uqnique devices at the nanoscale level possessing novel physical and chemical functional properties. However, the toxicities of nanomaterials have not been fully tested and the risk of nanomaterials is emerging issues in these days. In this study, the cytotoxicity of copper nanoparticles was tested in cultured human bronchial epithelial cells. As a results, copper nanoparticles showed cytotoxicity similar with cupric ion and the apoptotic mechanisms of DNA fragmentation and caspase-3 activation were involved. Induction of heme oxygenase-1 and thioredoxin reductase by copper nanoparticles indicated that cytotoxicity of copper nanoparticles is likely to be mediated through oxidative stress.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2011년 춘계학술대회논문집
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• pp.291-293
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• 2011

In this paper, the problem of transonic aerodynamic characteristics of a NACA0012 airfoil is numerically investigated in the inviscid gas-droplet two-phase flow with the compressible two-fluid model. In the present study, the airfoil flight in the cloud is simulated by taking account of the viscous drag of the droplets, the heat transfer, the phase change, and the droplet fragmentation The two-fluid equation system is solved by the fractional-step method and the WAF-HIL scheme. The effects of size and volume fraction of the droplets on the flow characteristics of the airfoil in the cloud are elaborated and discussed.