• Proceedings of the Zoological Society Korea Conference
• /
• 1995.10b
• /
• pp.171.1-171
• /
• 1995

• Proceedings of the Zoological Society Korea Conference
• /
• 1997.10b
• /
• pp.287.1-287
• /
• 1997

No Abstract, See Full Text

• 소프트웨어공모대전
• /
• s.5
• /
• pp.175-187
• /
• 2000

• The Korea Genome Conference
• /
• 2003.09a
• /
• pp.60.2-60.2
• /
• 2003

• Bulletin of the Korean Chemical Society
• /
• v.18 no.1
• /
• pp.3-5
• /
• 1997

• Bulletin of the Korean Chemical Society
• /
• v.17 no.12
• /
• pp.1107-1108
• /
• 1996

• Journal of Microbiology and Biotechnology
• /
• v.6 no.2
• /
• pp.142-144
• /
• 1996

To clone the gene coding for steroid $\Delta^1$-dehydrogenase of Arthrobacter simplex, its genomic library was constructed with a , $\lambda$gt11 expression vector and immunoscreened with antiserum against the enzyme. One positive clone was found to carry a 1.6-kb EcoR I restriction endonuclease fragment of A. simplex DNA. The restriction map of the 1.6-kb EcoR I fragment was determined after cloning of the DNA into pBS vector.

• Journal of Microbiology and Biotechnology
• /
• v.7 no.5
• /
• pp.299-304
• /
• 1997

Expression in the periplasm of Escherichia coli of cloned heavy and light chain cDNAs for Fab fragment of a murine monoclonal antibody MabB9 (${\gamma}2b$, K), specific for human plasma apolipoprotein B-100 of LDL, was studied. For the purpose, a vector for two-cistronic expression of the heavy chain cDNA, at the 5' terminus, and light chain cDNA, at the 3' terminus, was constructed using the signal sequences, pelB (for heavy chain) and ompA (for light chain) in a pET vector system. The constructed vector was transformed into E. coli BL21(DE3). The expressed heavy chain (25 kDa) and light chain (23 kDa) of the antibody molecule were detected in total cell extracts as well as in the periplasmic extracts of E. coli.

• Journal of Microbiology and Biotechnology
• /
• v.3 no.2
• /
• pp.91-94
• /
• 1993

A 3-isopropylmalate dehydrogenase (3-IPMD) gene was cloned from Kluyveromyces fragilis. pJK104 could complement Escherichia coli leuB and Saccharomyces cerevisiae leu2 auxotrophs. The coding region was subcloned and the nucleotide sequence was determined. A 1.8 Kb EcoRI/SphI fragment of pJK104 subcloned in pUC18 could still complement the leuB mutation. An open reading frame of 1164 bp that corresponds to a polypeptide of 387 amino acids was found in the cloned fragment. The homology between the 3-isopropylmalate dehydrogenase of S. cerevisiae and that of K. fragilis was 68.13％ in nucleotides.

• The Journal of Korean Society of Virology
• /
• v.26 no.2
• /
• pp.173-179
• /
• 1996

The gene encoding gIII of bovine herpesvirus type 1 (BHV-1) PQ strain was cloned and expressed in baculovirus. Although the gIII gene is located in Hind III I fragment as the case of the other BHV-1 strains, differences in size and restriction endonuclease site within the fragment were identified. The gIII expression was predominantly detected on the surface on insect cells by indirect immunofluoresecnce assay using monoclonal antibody. The western blotting analysis also revealed the presence of expressed protein of a similar molecular size to the original gIII protein. The immunogenicity of expressed protein were tested in guinea pigs. The immunized guinea pigs with expressed protein developed the neutralizing antibodies against BHV-1.