• Title/Summary/Keyword: fragment

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Nucleotide Sequence of a Truncated Proteinase Inhibitor I Gene of Potato (감자에서 분리된 절단형 단백질분해효소 억제제 I 유전자의 염기서열)

  • 이종섭
    • Journal of Plant Biology
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    • v.33 no.4
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    • pp.303-307
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    • 1990
  • A genomic clone carrying a proteinase inhibitor I sequence was isolated and characterized. The clone contained a 0.7 kb EcoRI fragment hybridized with tomato inhibitor I cDNA. The nucleotide sequence of the EcoRI fragment revealed presence of a truncated form of a proteinase inhibitor I gene of potato. The truncated gene contained the 5' flanking region and the first exon of a functional proteinase inhibitor I gene. Although the 5' flanking region contained the regulatory sequences TATAAA and CCACT, a deletion of 40 bp occurred between them.

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Effective Query Processing on Streamed XML Fragments (스트림된 XML 조각들의 효율적인 질의 처리)

  • Ko, Hye-Kyeong
    • Journal of Information Technology Services
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    • v.12 no.2
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    • pp.257-268
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    • 2013
  • Query processing on streamed XML fragments is one of key issues in XML databases. In this paper, XFSeed (XML Fragment Processor with Seed label) is proposed to provide effective query processing by removing many redundant path evaluations and minimizing the number of fragments processed. The conducted experimental results reveal that the proposed scheme efficiently handles query processing and reduces memory usage.

Bending of DNA by cAMP Receptor Protein(CRP)

  • 정수열
    • Journal of Life Science
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    • v.4 no.3
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    • pp.119-123
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    • 1994
  • CRP에 의한 DNA bending의 첫 실험은 cAMP, CRP, DNA complex의 gelelectrophoresis에 의해 확인되었다. Bent DNA fragment의 이동은 같은 크기의 linear fragment보다 느리며, EH cAMP, CRP, DNA 결합위치에 따라서 상이한 이동 pattern을 나타낸다. 즉, DNA 단편의 중앙에 CRP binding site가 있을 때는 말단부분에 있을 때보다 이동도가 느리다. benting각의 크기는 일반적으로 안정한 복합체의 형성에 관계하며, 큰 각을 갖는 것이 보다 더 안정된 구조를 형성하여 전사가 촉진되는 것으로 밝혀져 있다. 본고에서는 CRP에 의한 bending과 CRP에 의한 전사조절 관계와 bending의 관계에 대하여 알아보았다.

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Traumatic Separation of Bipartite Patella Underlying Gout

  • Choi, Eun Seok;Sim, Jae Ang;Go, Jae Yun;Na, Young Gon
    • Journal of Trauma and Injury
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    • v.31 no.3
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    • pp.189-193
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    • 2018
  • Gouty arthritis is a common crystal arthropathy, but gout tophus in the bipartite patella is a rare condition. This report presented a traumatic separation of bipartite patellar fragment caused by mild trauma in a patient with comorbid gout. When a patient with bipartite patella and underlying gouty arthritis complains of pain after trauma, clinical suspicion is needed about fragment separation of the bipartite patella.

A Cooperative Workflow Modeling Methodology Using Fragment-ICNs (조각-ICN 기반 협업 워크플로우 모델링 방법론)

  • Kim Hyung-Mok;Kim Kwang-Hoon
    • Journal of Internet Computing and Services
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    • v.6 no.6
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    • pp.107-115
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    • 2005
  • A workflow procedure has recently become more complicated and large scaled. In this paper, we propose an advanced workflow modeling methodology, called a fragment driven cooperative workflow modeling methodology. which enables several real workflow designers to cooperatively define a workflow model. The methodology is a Bottom-Up approach in terms of integrating a set of fragment ICNs to compose a complete workflow model. Each fragment ICN is defined by each participant in the cooperative modeling session, We also use the ICN based formal description and the ICN based graphical notation as well. Finally, we prove the feasibility of the methodology by implementing a cooperative workflow modeling system.

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Development of PCR-Based Sequence Characterized DNA Markers for the Identification and Detection, Genetic Diversity of Didymella bryoniae with Random Amplified polymorphic DNA(RAPD)

  • Kyo, Seo-Il;Shim, Chang-Ki;Kim, Dong-Kil;Baep, Dong-Won;Lee, Seon-Chul;Kim, Hee-Kyu
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.130-130
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    • 2003
  • Gummy stem blight pathogen is very difficult not only to monitor the inoculum levels prior to host infection, and also it is destructive and hard to control in field condition. We have applied RAPD technique to elucidate the genetic diversity of the genomic DNA of Didymella bryoniae and also to generate specific diagnostic DNA probe useful for identification and detection. The 40 primers produced clear bands consistently from the genomic DNA of twenty isolates of Didymella bryoniae, and two hundred seventy-three amplified fragments were produced with 40 primers. The combined data from 273 bands was analyzed by a cluster analysis using UPGMA method with an arithmetic average program of NTSYS-PC (Version 1.80) to generate a dendrogram. At the distance level of 0.7, two major RAPD groups were differentiated among 20 strains. RAPD group (RG) I included 8 isolates from watermelon except one isolate from melon. RAPD group (RG) IV included 12 isolates from squash, cucumber, watermelon and melon.. In amplification experiment with SCAR specific primer RG1F-RG1R resulted in a single band of 650bp fragment only for 8 isolates out of 20 isolates that should be designated as RAPD Group 1. However, same set of experiment done with RGIIF-RGIIR did not result in any amplified product.. Our attempts to detect intraspecific diversity of ITS region of rDNA by amplifying ITS region and 17s rDNA region for 20 isolates and restriction digestion of amplified fragment with 12 enzymes did not reveal polymorphic band. In order to develop RAPD markers for RGIV specific primer, a candidate PCR fragment( ≒1.4kb) was purified and Southern hybridized to the amplified fragment RGIV isolates. This promising candidate probe recognized only RGIV isolates

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A Simple and Efficient Antialiasing Method with the RUF buffer (RUF 버퍼를 이용한 간단하고 효율적인 안티알리아싱 기법)

  • 김병욱;박우찬;양성봉;한탁돈
    • Journal of KIISE:Computer Systems and Theory
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    • v.30 no.3_4
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    • pp.205-212
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    • 2003
  • In this paper, we propose a simple and efficient hardware-supported antialiasing algorithm and its rendering scheme. The proposed method can efficiently reduce the required memory bandwidth as well as memory size compared to a conventional supersampling when rendering 3D models. In addition, it can provide almost the same high quality scenes as supersampling does. In this paper, we have introduced the RUF (Recently Used Fragment) buffer that stores some or whole parts of a fragment or two more the merged results of fragments that recently used in color calculation. We have also proposed a color calculation algorithm to deteriorate the image quality as referencing the RUF buffer. Because of the efficiency presented in the proposed algorithm, the more number of sampling points increases the more memory saving ratio we can gain relative to the conventional supersampling. In our simulation, the proposed method can reduce the amount of memory size by 31% and the memory bandwidth by 11% with a moderate pixel color difference of 1.3% compared to supersampling for 8 sparse sampling points.

Construction of Large Library of Protein Fragments Using Inter Alpha-carbon Distance and Binet-Cauchy Distance (내부 알파탄소간 거리와 비네-코시 거리를 사용한 대규모 단백질 조각 라이브러리 구성)

  • Chi, Sang-mun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.19 no.12
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    • pp.3011-3016
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    • 2015
  • Representing protein three-dimensional structure by concatenating a sequence of protein fragments gives an efficient application in analysis, modeling, search, and prediction of protein structures. This paper investigated the effective combination of distance measures, which can exploit large protein structure database, in order to construct a protein fragment library representing native protein structures accurately. Clustering method was used to construct a protein fragment library. Initial clustering stage used inter alpha-carbon distance having low time complexity, and cluster extension stage used the combination of inter alpha-carbon distance, Binet-Cauchy distance, and root mean square deviation. Protein fragment library was constructed by leveraging large protein structure database using the proposed combination of distance measures. This library gives low root mean square deviation in the experiments representing protein structures with protein fragments.

Cloning, Nucleotide Sequence and Expression of Gene Coding for Poly-3-hydroxybutyric Acid (PHB) Synthase of Rhodobacter sphaeroides 2.4.1

  • Kim, Ji-Hoe;Lee, Jeong-Kug
    • Journal of Microbiology and Biotechnology
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    • v.7 no.4
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    • pp.229-236
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    • 1997
  • A gene, $phbC_{2.4.1}$ encoding poly-3-hydroxybutyric acid (PHB) synthase of Rhodobacter sphaeroides 2.4.1 was cloned by employing heterologous expression in Escherichia coli. R. sphaeroides chromosomal DNA partially digested with MboI was cloned in pUC19 followed by mobilization into E. coli harbouring $phbA,B_{AC}$ in pRK415, which code for ${\beta}$-ketothiolase and acetoacetyl CoA reductase of Alcaligenes eutrophus, respectively. Two E. coli clones carrying R. sphaeroides chromosomal fragment of $phbC_{2.4.1}$ in pUC19 were selected from ca. 10,000 colonies. The PHB-producing colonies had an opaque white appearance due to the intracellular accumulation of PHB. The structure of PHB produced by the recombinant E. coli as well as from R. sphaeroides 2.4.1 was confirmed by [$H^{+}$]-nuclear magnetic resonance (NMR) spectroscopy. Restriction analysis of the two pUC19 clones revealed that one insert DNA fragment is contained as a part of the other cloned fragment. An open reading frame of 601 amino acids of $phbC_{2.4.1}$ with approximate M.W. of 66 kDa was found from nucleotide sequence determination of the 2.8-kb SaiI-PstI restriction endonuclease fragment which had been narrowed down to support PHB synthesis through heterologous expression in the E. coli harbouring $phbA,B_{AC}$. The promoter (s) of the $phbC_{2.4.1}$ were localized within a 340-bp DNA region upstream of the $phbC_{2.4.1}$ start codon according to heterologous expression analysis.

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