• 제목/요약/키워드: fraction analysis

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토양 중 브롬화다이옥신류의 분석법 고찰

  • 김태승;신선경;이정희
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.04a
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    • pp.413-417
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    • 2003
  • The last few decades have seen drastic growth in the use of brominated flame retardants(BFRs). There is the problems of polybrominated dibenzo-p-dioxins(PBDDS) and polybrominated dibenzofurans(PBDFs) generation as by-products in combustion process of BFRs. PBDDs/PBDFs are highlighted the new pollutants of environmental contamination, recently. In this study, the 10 kinds of PBDDs/PBDFs standards were used to perform the experiments of recoveries. The analysis of PBDDs/PBDFs in soil environmental samples. was carried out. In silica gel column cleanup, PBDDs/PBDFs eluted until 80mL of n-hexane, and the recovery was obtained 73∼105%. In multi-layer silica gel column cleanup, the recovery was obtained 56∼125% with various elution solvents. In alumina clean process, the standard mixture(PBDES, PBDDs/PBDFs) eluted the first fraction of 2% dichloromethane with n-hexane in the range of 0∼100mL, PBDEs compounds eluted in the second fraction of 50% dichloromethane with n-hexane. In activated carbon column cleanup, the PBBEs eluted to the first fraction(n-hexane) and second fraction(toluene), but PBDDs/PBDFs only eluted to the second fraction. The analytical methods of PBDDs/PBDFs for soil was established based on the experimental results of the environmental samples.

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Extraction and Bioassay of Allelochemicals in Jerusalem Artichoke

  • Sungwook Chae;Lee, Ho-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.4
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    • pp.309-316
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    • 2001
  • Helianthus tuberosus has been known to inhibit the growth of weeds and other plants sharing its habitat. This study was conducted to identify the allelochemicals of Helianthus tuberosus which were extracted with water and solvents. Aqueous extracts of leaf, stem, root, tuber and tuber peel of Helianthus tuberosus except tuber did not show significant differences in phytotoxicity to alfalfa seedlings. It was considered that Helianthus tuberosus contained fewer or less potential water-soluble substances that were toxic to alfalfa. Methanol extract of leaves of Helianthus tuberosus was sequentially partitioned in increasing polarity with n-hexane, ethylacetate and n-butanol. Each extract had a yield of 148, 12, 15.7 and 9.5g, respectively. Inhibitory effects on germination of alfalfa seeds treated with four fractions were not significantly different. But the significant reductions on hypocotyl length were observed for all the solvent extracts. Among the four fractions, the ethylacetate fraction showed the most significant inhibition effect on bioassay with alfalfa. Further separation of the active ethylacetate fraction by open column chromatography led to the 25 subfractions. In bioassay of each sub-fraction with alfalfa seeds, sub-fraction No. 13 showed the most inhibitory effect on seedling growth. $^1$H NMR and gas chromatography-mass spectrometry analysis revealed that sub-fraction No. 13 was the mixture of straight-chain saturated fatty acids.

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An Analysis of Third Graders' Understanding on the Part-Whole Fraction Concept (3학년 학생들의 전체-부분으로서의 분수에 대한 이해 분석)

  • Kim, Yu-Kyung;Pang, Jeong-Suk
    • Journal of Educational Research in Mathematics
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    • v.22 no.3
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    • pp.311-329
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    • 2012
  • This study analyzed third graders' understanding on the part-whole fraction concept in both the continuous and the discrete contexts. A set of problems were developed as an equivalent form to compare and contrast students' understanding of fraction in the two contexts. Unexpectedly, the results of this study showed that students' performance in the continuous contexts was slightly lower than their performance in the discrete contexts. Students tended to use different strategies depending on the contexts and they had difficulties in applying what they knew in the new contexts. On the basis of the detailed analyses about students' difficulties and their sources, this paper provides information on how to construct curricular materials and how to teach the basic concepts related to the part-whole fraction.

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Experimental Study on Two-Phase Flow Parameters of Subcoolet Boiling in Inclined Annulus

  • Lee, Tae-Ho;Kim, Moon-Oh;Park, Goon-Cherl
    • Nuclear Engineering and Technology
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    • v.31 no.1
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    • pp.29-48
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    • 1999
  • Local two-phase flow parameters of subcooled flow boiling in inclined annulus were measured to investigate the effect of inclination on the internal flow structure. Two-conductivity probe technique was applied to measure local gas phasic parameters, including void fraction, vapor bubble frequency, chord length, vapor bubble velocity and interfacial area concentration. Local liquid velocity was measured by Pilot tube. Experiments were conducted for three angles of inclination; 0$^{\circ}$(vertical), 30$^{\circ}$, 60$^{\circ}$. The system pressure was maintained at atmospheric pressure. The range of average void fraction was up to 10% and the average liquid superficial velocities were less than 1.3 m/sec. The results of experiments showed that the distributions of two-phase How parameters were influenced by the angle of channel inclination. Especially, the void fraction and chord length distributions were strongly affected by the increase of inclination angle, and flow pattern transition to slug flow was observed depending on the How conditions. The profiles of vapor velocity, liquid velocity and interfacial area concentration were found to be affected by the non-symmetric bubble size distribution in inclined channel. Using the measured distributions of local phasic parameters, an analysis for predicting average void fraction was performed based on the drift flux model and flowing volumetric concentration. And it was demonstrated that the average void fraction can be more appropriately presented in terms of flowing volumetric concentration.

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Numerical Analysis for Two-Dimensional Compressible and Two-Phase Flow Fields of Air-Water in Eulerian Grid Framework (2차원 압축공기-물의 압축성 이상 유동 수치 해석)

  • Park, Chan-Wook;Lee, Sung-Su
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.32 no.6
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    • pp.429-445
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    • 2008
  • Two-phase compressible flow fields of air-water are investigated numerically in the fixed Eulerian grid framework. The phase interface is captured via volume fractions of each phase. A way to model two phase compressible flows as a single phase one is found based on an equivalent equation of states of Tait's type for a multiphase cell. The equivalent single phase field is discretized using the Roe‘s approximate Riemann solver. Two approaches are tried to suppress the pressure oscillation phenomena at the phase interface, a passive advection of volume fraction and a direct pressure relaxation with the compressible form of volume fraction equation. The direct pressure equalizing method suppresses pressure oscillation successfully and generates sharp discontinuities, transmitting and reflecting acoustic waves naturally at the phase interface. In discretizing the compressible form of volume fraction equation, phase interfaces are geometrically reconstructed to minimize the numerical diffusion of volume fraction and relevant variables. The motion of a projectile in a water-filled tube which is fired by the release of highly pressurized air is simulated presuming the flow field as a two dimensional one, and several design factors affecting the projectile movement are investigated.

Melanogenesis-Inhibitory Effects of Ethyl Acetate Fraction from Diospyros lotus L. Leaf Extract (고욤(Diospyros lotus L.)잎 Ethyl Acetate 분획물의 멜라닌형성 억제 효과)

  • Choi, Byung-Min;Jeon, In Hwa;Kim, Sang Jun;Yu, Kang-Yeol;Jang, Seon Il
    • Korean Journal of Pharmacognosy
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    • v.45 no.3
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    • pp.220-226
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    • 2014
  • Diospyros lotus has been cultivated for its edible fruits and leaves which are considered for its medicinal importance. The aim of this study was to evaluate the anti-melanogenesis of ethyl acetate (EA) fractions from D. lotus leaves in B16 cells. The order of the total polyphenol content with regard to the different solvent fractions from D. lotus leaves was EA>butanol>metahanol>chloroform>n-hexane. The major compounds of EA fraction from D. lotus leaves by HPLC analysis were myricitrin and myricetin. Cellular TYR activity and melanin content in response to treatment with 100 mg/mL of EA fraction was inhibited more strongly than group treated with arbutin. Further, EA fraction exhibited significant anti-melanogenesis effects by reducing the levels of microphthalima-associated transcription factor (MITE), inhibiting the synthesis of TYR, tyrosinase-related protein-1 (TRP-1) and TRP-2. Therefore, EA fractions from D. lotus leaves may be a good source of skin-whitening agents in the future development of medicine-based trouble skin therapy.

Determinants of the Volumetric Markers of Left Atrial Contraction Function in Coronary Artery Disease: A Cross-sectional Study

  • Taimoor Etemad;Ali Hosseinsabet;Negar Omidi;Reza Mohseni-Badalabadi
    • Journal of Cardiovascular Imaging
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    • v.30 no.1
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    • pp.37-46
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    • 2022
  • BACKGROUND: A body of research advocates the prognostic role and usefulness of the volumetric markers of left atrial (LA) phasic functions in the diagnosis of LA dysfunction. We aimed to determine the independent determinants of the volumetric markers of LA contraction function in candidates for coronary artery bypass graft (CABG) surgery. METHODS: This cross-sectional study enrolled 516 candidates for CABG. The biplane maximal, minimal, and pre-P volumes of the LA were measured with two-dimensional echocardiography, and LA active emptying fraction was calculated. The standardized correlation coefficient for the correlation between each factor and LA active emptying fraction was calculated by using univariate and backward multivariable regression analyses. RESULTS: The multivariable regression analysis demonstrated that the heart rate (β = 0.15; p = 0.001), S (β = 0.09; p = 0.036), E/e' ratio (β = -0.11; p = 0.014), left ventricle (LV) ejection fraction (β = 0.15; p = 0.001), and LA enlargement (β = -0.19; p < 0.001) were the independent determinants of LA active emptying fraction. CONCLUSIONS: The independent determinants of LA contraction function were the heart rate, S, LV ejection fraction, LA enlargement, and E/e' ratio in candidates for CABG surgery.

Ruminal Protein Degradation Characteristics of Cell Mass from Lysine Production

  • Seo, S.;Kim, H.J.;Lee, S.Y.;Ha, Jong K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.3
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    • pp.364-370
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    • 2008
  • Chemical analysis and in vitro studies were conducted to investigate the nutritive value for ruminants of cell mass from lysine production (CMLP) which is a by-product of the lysine manufacturing process. Proximate analysis, protein fractionation, and in vitro protein degradation using protease from Streptomyces griseus and strained ruminal fluid were carried out to estimate ruminal protein degradability of CMLP with two reference feedstuffs-soybean meal (SBM) and fish meal (FM). Amino acid composition and pepsin-HCl degradability were also determined to evaluate postruminal availability. CMLP contained 67.8% crude protein with a major portion being soluble form (45.4% CP) which was composed of mainly ammonium nitrogen (81.8% soluble CP). The amount of nucleic acids was low (1.15% DM). The total amount of amino acids contained in CMLP was 40.60% DM, which was lower than SBM (47.69% DM) or FM (54.08% DM). CMLP was composed of mainly fraction A and fraction B2, while the protein fraction in SBM was mostly B2 and FM contained high proportions of B2 and B3 fractions. The proportion of B3 fraction, slowly degradable protein, in CP was the highest in fish meal (23.34%), followed by CMLP (7.68%) and SBM (1.46%). CMLP was degraded up to 51.40% at 18 h of incubation with Streptomyces protease, which was low compared to FM (55.23%) and SBM (83.01%). This may be due to the insoluble portion of CMLP protein being hardly degradable by the protease. The in vitro fermentation by strained ruminal fluid showed that the amount of soluble fraction was larger in CMLP (40.6%) than in SBM (17.8%). However, because the degradation rate constant of the potentially degradable fraction of CMLP (2.0%/h) was lower than that of SBM (5.8%/h), the effective ruminal protein degradability of CMLP (46.95%) was slightly lower than SBM (53.77%). Unavailable fraction in the rumen was higher in CMLP (34.0%) compared to SBM (8.8%). In vitro CP degradability of CMLP by pepsin was 80.37%, which was lower than SBM (94.42%) and FM (89.04%). The evaluation of protein degradability using different approaches indicated that soluble protein in CMLP may supply a large amount of ammonia in the rumen while insoluble protein can be by-passed from microbial attacks due to its low degradability. The results from this study suggest that CMLP can be used as a protein supplement to ruminants for supplying both non-protein nitrogen to rumen microbes and rumen undegradable protein to the host animal.

Cellular Protective Effect and Component Analysis of Euphorbia humifusa Extracts (땅빈대 추출물의 세포 보호 효과 및 성분 분석에 관한 연구)

  • Kim, Sun-Young;Won, Doo-Hyun;Lim, Myoung-Sun;Park, Soo-Nam
    • Korean Journal of Pharmacognosy
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    • v.41 no.4
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    • pp.264-269
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    • 2010
  • In this study, the cellular protective effect, antioxidative property and component analysis of Euphorbia humifusa extracts were investigated. The ethyl acetate fraction ($3.68\;{\mu}g/mL$) and aglycone fraction ($3.15\;{\mu}g/mL$) of Euphorbia humifusa extract showed prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity ($FSC_{50}$). Reactive oxygen species (ROS) scavenging activity ($OSC_{50}$) of Euphorbia humifusa extract on ROS generated in $Fe^{3+}$-EDTA/$H_2O_2$ system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction ($0.43\;{\mu}g/mL$) and aglycone fraction ($0.35\;{\mu}g/mL$) of extract showed higher ROS scavenging activity than L-ascorbic acid ($1.50\;{\mu}g/mL$). The cellular protective effects of fractions of Euphorbia humifusa extract on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction and aglycone fraction of extract protected cellular membranes against ROS in a concentration dependent manner ($5{\sim}25\;{\mu}g/mL$), and was more effective than (+)-${\alpha}$-tocopherol, lipid peroxidation chain blocker. Aglycone fraction from Euphorbia humifusa extract showed 2 bands in TLC and 2 peaks in HPLC. In HPLC chromatogram of aglycone fraction, peak 1 and peak 2 were identified as quercetin and kaempferol, respectively. And these components are very effective as antioxidant. Thus, these results indicate that fractions of Euphorbia humifusa extracts can function as antioxidant in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. Fractions of Euphorbia humifusa extracts can be applicable to new functional cosmetics for antioxidant.

Method for Determining Fiber Volume Fraction in Carbon/Epoxy Composites Considering Oxidation of Carbon Fiber (탄소섬유 산화 현상을 고려한 탄소복합재료의 섬유체적비 측정법)

  • Kim, YunHo;Kumar, Sathish;Choi, Chunghyeon;Kim, Chun-Gon;Kim, Sun-Won;Lim, Jae Hyuk
    • Composites Research
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    • v.28 no.5
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    • pp.311-315
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    • 2015
  • Measuring fiber volume fraction properly is very important in designing composite materials because the fiber volume fraction mainly determines mechanical and thermal properties. Conventional Ignition methods are effective for ceramic fiber reinforcing composite materials. However, these methods are not proper for applying to carbon fiber reinforcing composites because of the venerable characteristic against oxidation of carbon fiber. In the research, fiber volume fraction of carbon fiber composites was obtained by a thermogravimetric analysis considering oxidation characteristic of the carbon fiber and the method was compared and verified with the results from microscopic cross section images.