• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.141-146
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• 1997

Postmortem examination was conducted on 350 (three hundred and fifty) chickens. Related samples (Liver, heart, ovary, spleen, bone-marrow, and caecal junction) were collected. The appropriate materials from the samples were cultured into different media. A total 40(forty) isolates of salmonella pullorum and S. gallinarum were identified and preserved. Characterization of the isolates were done by cultural, morphological, biochemical, and serological tests. Salmonella pullorum antigen was prepared from the local isolate, standardized and tested. This antigen was used in the field for the detection of pullorum or fowl typhoid infection or carrier birds. The antigen consisted of suspension of Salmonella pullorum in 0.50 percent sodium chloride plus 1.5 percent sodium sulfate and inactivated with 1% formalin U.S.P. and standardized with McFarland scale iv or by pour plate method containing 800 million organisms per milliliter and stained by the addition of alcoholic crystal violet. Sterility, safety and potency were tested and found as good as other international antigens. The antigen was found to retain its quality for six months when preserved at room temperatures. The test was made by mixing one drop of the antigen with a drop of blood or a drop of serum, on a glass plate or white tile. The locally produced antigen was as good as antigens from Japan, Hungary, Holland and India. A serological study was conducted with the locally prepared antigen in different farms, and the incidence was 0-4% in government farms, 5-10% in commercial imported breeds and 0-3% in cross breed local farms respectively.

• Korean Journal of Microbiology
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• v.51 no.2
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• pp.177-180
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• 2015

For prevention of atrophic rhinitis of swine by Bordetella bronchiseptica and fowl typhoid by Salmonella gallinarum, bacterial strains showing antimicrobial activity against those pathogenic bacteria were isolated from various samples collected at animal farms. Among 372 bacterial isolates strain TK3 showed the highest antibacterial activity against both pathogens, and was identified as Bacillus amyloliquefaciens by 16S rRNA gene sequence analysis. B. amyloliquefaciens TK3 could inhibit growth of both pathogens by secretion of antibacterial compounds such as siderophore, rhamnolipid and antimicrobial peptide. Production radius of siderophore on Chrome azurol S agar plate by strain TK3 was 0.53 cm after 14 days of incubation, and concentration of siderophore in King's B medium was 1.06 mmol/ml. It also secreted 82.4 mg/L of rhamnolipid, and antimicrobial peptide that completely inhibited growth of both pathogens at concentration of $30{\mu}l/ml$ in LB medium.

• Korean Journal of Veterinary Service
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• v.40 no.2
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• pp.89-94
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• 2017

This study was conducted from May to December 2016 to investigate the prevalence of antibody titers for Newcastle disease (ND), Infectious Bronchitis (IB), Egg drop syndrome (EDS), Avian pneumovirus (APV), Fowl Typhoid (FT), Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS) in laying hens in Incheon area. In ND, 418 (99.5%) of the 420 outbreaks were positive. IB showed 330 (78.5%) positive antibody out of 420 cases. EDS showed 419 (99.7%) positive seropositive rates among 420 cases. In the case of APV, 357 cases (85%) were positive in 420 cases. In the case of PT, the positive rate of antibody was 128 (30.4%) out of 420 cases. Among the 420 cases of MG, 222 cases (52.8%) were positive for antibodies. In the case of MS, 395 out of 420 cases (94.0%) were positive. This result suggests that the positive rate of antibody for PT in Incheon area was low. And it was also clear that MS without vaccine is now rampant in Incheon.

• Korean Journal of Veterinary Research
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• v.64 no.1
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• pp.7.1-7.6
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• 2024

Bird species, particularly poultry and other bird types, including domestic pigeons, are susceptible to fowl pox, a contagious viral disease. The main goal of this study was to validate clinical avipoxvirus diagnoses using molecular analytical methods. The essential components of the investigation were the clinical signs, visible abnormalities, histological changes, and polymerase chain reaction analysis. Twenty out of 120 pigeons had clinical symptoms, which included yellowish crust or nodules near the feet, eyes, and beak. An erosive epidermal lesion and an epidermal acanthotic papular lesion with basal vacuolation were maculopapular evidence associated with significant epidermal hyperkeratosis, as confirmed by histological analysis. In addition, the results showed keratinocyte necrosis beneath the hyperkeratotic epidermal layer, together with superficial and deep dermal perivascular lymphocytic infiltration. In addition, the P4b core protein gene underwent phylogenetic analysis. The sequence analysis results indicated a high degree of similarity across the local strains, with just minor variations observed. Five sample sequences were selected and submitted to the NCBI database. These sequences were identified as OR187728, OR187729, OR187730, OR187731, and OR187732. All the various strains in this research may be classified under clade A of the chicken pox virus phylogenetic classification. This study presents the first description and characterization of pox virus infections in domestic pigeons inside the Basrah governorate.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.3
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• pp.335-340
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• 2010

This study was conducted to select organic materials (OM) and nitrogen sources in composting of waste mushroom bed from Agaricus bisporus. We examined physio-chemical properties of the organic materials and the mixture ratio for preparing the wasted mushroom bed (M) compost. The carbon content of sawdust was higher than those of rice straw (R) as OM source and the nitrogen content was high in the order of fowl manure (F)>> pig manure (P)> cow manure (C). The compost was prepared to maintain the criteria of above 25% organic matter and then the change of their ingredients was estimated during the process of fermentation. The temperature of waste mushroom bed+pig manure+rice straw (MRP) treatment was varied fast throughout fermentation, on the other hand the temperature of waste mushroom bed+pig manure+sawdust (MSP) treatment was steadily elevated to the middle of composting. The pH of the compost was somewhat high to pH 8.5~9.0 at the early stage, but decreased to 7.5 at the end stage of composting. The content of OM after fermentation was decreased to the level of 19~21% in rice straw, but the sawdust treatment maintained 25~27% organic matter. The waste mushroom bed+fowl manure+rice straw (MRF) treatment, which contains 26.2% organic matter and 0.68% nitrogen, was the highest among them. The volume of compost was reduced to 50% by using rice straw as organic matter, but reduced to 30% by using the sawdust. The contents of heavy metal in the compost were suitable within the legal criteria. The number of microorganisms were higher in the rice straw than those in the sawdust. It was high in the order of fowl manure> pig manure> cow manure. The major groups consisted of aerobic bacteria, gram negative bacteria and Bacillus sp. and their populations after fermentation were increased to $1{\times}10^1{\sim}1{\times}10^2\;cfu\;g^{-1}$ rather than those before fermentation. Therefore we concluded that the waste mushroom bed+fowl manure+sawdust (MSF 3:9:1 v/v/v) treatment was suitable combination for high organic matter and nitrogen source, and the periods of composting were 50~60 days.

• Korean Journal of Poultry Science
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• v.10 no.2
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• pp.123-128
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• 1983

Fifty of one day-old chicks of silky fowl were hatched at Dept. of Animal Science, Gyeongsang National University and fifty of one day-old chicks of quails were hatched at Gim Hae, Gyung Nam. This experiment was investigated at 24 hours after incubation. Twee items investigated in this experiment were the live weights, the weights of head, eye-ball, brain, heart, liver, lung, kidney, stomach, small intestine, large intestine, and yolk sac. The results obtained are summarized as follows; 1, Average weights of body, head, eye-ball brain, heart, liver, lung, kidney, stomach, small intestine, large intesine and yolk sac of the quail chicks and standard errors were 6.54${\pm}$0.02g, 1.38${\pm}$0.l3g, 0.29${\pm}$0.004g, 0.24${\pm}$0.006g, 0.08${\pm}$0.002g, 0.21${\pm}$0.005g, 0.06${\pm}$0.002g, 0.05${\pm}$0. 002g, 0.46${\pm}$0.010g, 0.24${\pm}$0.008g, 0.10${\pm}$0.004g, and 0.28${\pm}$0.0l6g, respectively. 2. Average weights of body, head, eye-ball, brain, heart, liver, lung, kidney, stomach, small intestine, large intestine, and yolk sac of the silky fowl chicks and standard errors were 26.60${\pm}$0.491g, 4.28${\pm}$0.071g, 0.64${\pm}$0.039g, 0.55${\pm}$0.025g, 0.21${\pm}$0.0l2g, 0.77${\pm}$0.030g, 0.13${\pm}$0.006g, 0.08${\pm}$0.006g, 1,53${\pm}$0.076g 0.73${\pm}$0.036g, 0.42${\pm}$0.031g, and 2.65${\pm}$0.297g, respectively. 3. The relative ratios of the organ weight versus body weight in guails were compared with those of the commercial chicks (Hibro, Hubbard, and Hisex) and silky fowls. It was noted that the relative weights of the brain and eye ball with respect to body weight were larger in case of quails (Table 3). The weights of heart, liver, lung, kidney, stomach and small intestine in silky fowl were smaller than those of commercial chicks and quail chicks. Other items were similar in weight to commercial chicks ana quail chicks.

• Korean Journal of Poultry Science
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• v.37 no.3
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• pp.255-263
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• 2010

Inclusion body hepatitis-hydropericardium syndrome (IBH-HPS) is an acute viral disease usually found in broilers aged from 3 to 5 weeks and causes up to 75% mortality. Among the 12 serotypes of fowl adenovirus group 1, serotype-4 (FAdV-4) was identified as a primary agent of IBH-HPS and was usually isolated in IBH-HPS cases in Korea since 2007. To prevent these IBH-HPS outbreaks in Korea, we developed the FAdV-4 inactivated vaccine using Korean isolate (ADL070244) and evaluated the efficacy of this vaccine. For the efficacy test, 2-week-old specific-pathogen-free (SPF) chickens intramuscularly inoculated with 1 or 2 dose of inactivated vaccine were used and challenged with FAdV-4 through either intramuscular or oral route at 2 weeks after vaccination. The vaccine induced good seroconversion which was confirmed by agar gel precipitation (AGP) test. In addition, the vaccine could decrease the FAdV-4 detection rate and histological lesion severity such as lymphocyte infiltration and necrosis in the liver comparing with those of non-vaccination group. Based on the current results, the developed FAdV-4 inactivated vaccine in this study was effective in the terms of reduction of virus detection rate and histological lesions severity. However, it was difficult to confirm the efficacy of the vaccine clearly because of no mortality and clinical signs in the non-vaccinated group after challenge. Therefore, we need further study to develop a standard challenged model system which could clearly evaluate the efficacy of the vaccines for FAdV-4.

• Korean Journal of Poultry Science
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• v.37 no.3
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• pp.237-245
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• 2010

A survey of transovarially transmitted diseases, including salmonellosis [(pullorum disease; PD)/(fowl typhoid; FT)], mycoplasmosis, avian infectious anemia (CIA), and fowl adenovirus infection was conducted in the breeder chickens from August to December in 2009. The numbers of flocks sampled out were: Grand Parents Stock (GPS), 45; Parents Stock (PS) 1,018, Baeksemi breeder (BSB) 54. The seroprevalence of salmonellosis (PD/FT) were 0% (GPS), 3.2% (PS), and 3% (BSB), respectively. A total of 983 chicken farms were affected with FT outbreaks between 2000 and 2008. The incidence of FT in commercial broilers, Baeksemi, commercial layers, native chickens, and broiler breeders was 44.3%, 26.2%, 15.7%, 12.6% and 1.08%, respectively. Of the affected broilers, over 90% birds were under 2 weeks of age, indicating it was possible that they were infected with S. gallinarum via vertical transmission. The sero-positive flocks against Mycoplasma gallisepticum (MG) were 71.1% (GPS), 88.7% (PS), 88.7% (BSB), while the rates of positive flocks against Mycoplasma synoviae (MS) were 86.0% (GPS), 77.0% (PS), and 98.0% (BSB). In GP and parent farms, the detection rates on specific genes of CIA virus were 19/45 (42.2%), and 169/1039 (18.0%), respectively, whereas the seroprevalence of CIA were 86.0% in GPS and 93.7% in PS flocks. In addition, positive flocks of fowl adenoviruses were 4.4% (GPS), 2.7% (PS) and 9.35% (BSB), respectively. As the results, avian mycoplasmosis and CIA have been more prevailing in chicken breeder than avian salmonellosis and fowl adenovirus infection in Korea.

• Korean Journal of Poultry Science
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• v.19 no.4
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• pp.205-215
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• 1992

This study investigated the characteristics of Korean Native Fowl (KW) to standarize the feeding regime and compared with those of broiler. The characteristics of KNF studied were meat productivities and carcass traits. The data were collected for 14 weeks from March to June, 1992, by recording from 200 birds(50 birds per each sex and 2 breed) were as follows ： 1. Average body weight at 14 weeks of age of Korean Native Fowl(KW) and broiler were 868.7g and 3,526.6g, respectively and were 26.6 times and 72.9 times of day old chicks weight averages, respectively. 2. The carcass yields during 9∼14 wks were 74.1∼78.5％ for KNF male, 73.g∼77.5％ for KW female, 72.2∼76.9％ for broiler male and 71.7∼76.4％ for female. It was shown that carcass yields of KNF was 2％ higher than that of broiler. 3. In edible portion, the yields of wings weight to carcass weight were 11.5∼12.4％ for KNF male, 11.5∼13.1％ for KNF female, 10.7∼11.9％ for broiler male and 10.7∼12.2％ for broiler female, it was shown that wings weight of KW was higher than that of broiler and wings weight of KNF during 9∼14 wks. 4. The portions of back and breast weight were higher than those of KNF during 9∼14 wks. The back portions in carcass weight were 15.4∼17.0％ for KW male 15.4∼16.8％ for KNF female, 17.2∼19.0％ for broiler male, and 17.2∼19.0％ for broiler female. The breast portions in carcass weight were 19.6∼22.0％ for KNF male, 20.2∼22.7％ for KNF female 23.S∼24.5％ for broiler male, and 25.1∼27.7％ for broiler female. 5. The portion of thigh and drumsticks weight in carcass weight of KNF was similar to that of broiler. They were 26.8∼28.0％ for KNF male, 25.5∼27.1％ for KNF female, 27.7％ for broiler male, and 26.9％ for broiler female, respectively. 6. For the weight of inedible portion during 9∼14 wks, feather weight of KNF was 5.3∼6.8％ of live weight and 3.8 ∼4.4％ in those of broiler. This result indicated that KNF has more rooms for improvement than broiler. 7. Abdominal fat weight was 2.42∼3.88g for KNF male and 1.84∼3.08g for KNF female, which was 0.5％ of carcass weight. But broiler has more abdominal fat than KNF as 39.4∼56.3 g for male, 33.3∼67.8 g for female which was 2.5 ∼3.0％ of carcass weight.

• Korean Journal of Poultry Science
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• v.30 no.1
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• pp.1-10
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• 2003

The objects of this study were to develop a new strain which has resistance to fowl typhoid, high performance in laying, and producing brown shell eggs favored by domestic consumers. Several White Leghorn (WL) breeds known as possessing genetic resistance to fowl typhoid and several brown shell egg breeds such as susceptible to the disease were used to produce the controlled strains with cross mating between the strains within the breeds and the experimental strains with crossbreeding between the breeds. The crossbred strains were Chungnong(CN) 21, 22, 23, 24, 25 and 26. The controlled strains were ISA brown CC, Hyline brown CC and Lohman brown CC. The survival rates were 99.95% for chicks of age 0∼l7 weeks and 91% for adult chickens of age 18∼72 weeks. There was no difference in survival rate between the crossbred and the controlled strains. The means of age of lst egg laying were 147 to 148 days and no difference was observed between the crossbred and the controlled. The egg Production rates of a9e 18∼72 weeks were 83.76% far the crossbred strains and 77.82% for the controlled strains, which is significantly higher in the crossbred than controlled strains by 6%. The numbers of the hen housed egg Production of age 18∼72 weeks were 292.33 eggs for the crossbred strains and 271.31 eggs fur the controlled strains. The difference of 21 eggs more produced by the crossbred than by the controlled was statistically significant. The mean egg weights of age 18∼72 weeks were 64.32g for the crossbred strains and 60.73g for the controlled strains, and the difference of 3.59g was statistically significant. The feed conversion rates during the age of 18 to 72 weeks were 2.297 for the crossbred strains and 2.454 for the controlled strains. The crossbreds were reduced feed consumption by 157g for 1kg of egg Production which was statistically significant. Haugh unit(H$.$u) at the age of 72 weeks were 82.20 for the crossbred strains and 77.82 for the controlled strains. The crossbred strains were superior quality by 4.38 H$.$u. There were no significant differences in the yolk color and the eggshell thickness between the crossbred strains and the controlled strains. The eggshell color of the crossbred strains was light brown which is the medium color of the white eggshell strains and the brown eggshell strains. The body weights at the ages of 8, 12, 18, 42 and 71 weeks were not different between the crossbred strains and the controlled strains.