• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.17-17
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• 2003

The milk of transgenic animals may provide an attractive vehicle for large-scale production of hEPO. Since glycosylation is cell type specific, recombinant human EPO (rhEPO) produced in different host cells contain different patterns of oligosaccharides, which could affect the biological functions. However, there have been no reports on the characteristics of rhEPO derived from milk of transgenic animals. To address this objective, several transgenic mice by using pWAPhEPO and/or pBC1hEPO expression vector were produced. However, 2 lines of pWAPhEPO founder female mouse died during late gestational day (day 18) before offspring could be obtained. They showed a severe splenomegaly, Unlike those of pWAPhEPO, mammary gland epithelial cells from biopsies of lactating pBC1hEPO transgenic mice had marked immunoreactivity to EPO and any activity was not detected in other tissues. The expression level of rhEPO is about 0.7% of mammary gland cellular total soluble proteins and an amount of 300~500 mg/L rhEPO is secreted into milk. Furthermore, the pBC1hEPO transgenic mice transmitted this character to their progeny in mendelian manner. In order to determine the extent of glycosylation variation, N-linked oligosaccharide structures present in the milk-derived rhEPO were characterized. Most of milk-derived rhEPO is fully glycosylated. the biological activity of milk-derived rhEPO was comparable to that of purified CHO-derived rhEPO, and milk-derived rhEPO showed relatively stable after freezing and thawing. Taken together, the results illustrate the potential of transgenic animals in the large-scale production of biopharmaceuticals.

• 한국실내디자인학회논문집
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• 제23권5호
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• pp.165-173
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• 2014

Located in Dornach, Switcherland Eurythmeum Anbau zu Haus Brodbeck (Rudolf Steiner-Halde, Eurythmeum Anbau zu Haus Brodbeck, 1923-1935) is one of the architectural works created by Rudolf Steiner (1861-1925) who has studied and established the cognitive and spiritual aspects of a human being, and is often cited as being the founder of anthroposophy. In fact, Eurythmeum Anbau zu Haus Brodbeck is not as well known as Steiner's other works, and it is perceived as less important than his major works of architecture including "Goetheanum", "Modellbau zu Malsch", and "Rudolf Steiner Haus Stuttgart". Nonetheless, this study is meaningful in itself since it is an important piece of architecture to gain an understanding of the characteristics of Steiner's late works, and the architectural characteristics of the Waldorf School where various active educational activities are taking place around the world. Eurythmeum Anbau zu Haus Brodbeck clearly exhibits the characteristics of the architectural space based on Steiner's anthroposophy as well as provides a window into promoting space efficiency by extending an existing building. Moreover, it provides a good learning opportunity to find out about how Steiner's architectural disposition has changed and developed with the use of new materials. With these points as a backdrop, the study aims to develop an understanding of the architectural characteristics of Eurythmeum Anbau zu Haus Brodbeck. Another important objective of this paper is to gain insight into the architectural implications in connection with the influence Eurythmeum Anbau zu Haus Brodbeck has had on Steiner's later works, by comparing his early works of architecture with those of his late works.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.47-47
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• 2001

The neuropeptide vasopressin (VP) is a nine- amino acid hormone synthesized as preprohormone in the cell bodies of hypothalamic magnocellular neurons. The tumor in magnocellular neurons of the hypothalamus is associated with disfunctions of the cell bodies, leading to the diabetes insipidus. In order to study with the diabetes insipidus caused by a defect in VP synthesis and its secretion, we have produced the transgenic mice regulated by vasopressin promoter inserted to SV40 T antigen coding sequence (pVPSV.IGR2.1). One transgenic line expressing high levels of SV40 T antigen was propagated. The founder and all transgene positive adult animals have appeared with shorten mortality or apparent phenotypic abnormalities, including immune complex disease, and eventually die between 4 and 8 months of age. The mRNA and protein of SV40T antigen transgene were detected in brain of fetus as well as in brain, spleen, lung and lymph node in moribund at the age of 20 weeks. Histological analysis of transgenic mice showed that tumor developed in brain similar to primitive neuroectodermal tumors (PNET) in man. We also detected lymphomas in spleen and lymph node, and consequent tumor formation in various tissues of the transgenic mice. In pVPSV.IGR2.1, 21% mice showed brain tumor (PNET) at 5 weeks and 100% mice showed brain tumor after 15 weeks. In addition, Expression of apoptosis related genes (Bcl-28 & Bax) was increased over their age in mice with PNET as compared to control mice. Apoptosis related gene expression might be deregulated in mice with brain tumor. However, transgenic mice were not developed with the diabetes insipidus. These mice represent the first disease model to exhibit primitive neuroectodermal tumor in brain, as well as a unique model system for exploring the cellular pathogenesis of lymphomas.

• Reproductive and Developmental Biology
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• 제30권2호
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• pp.93-97
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• 2006

To study the signaling effect of insulin-like growth factor-I(IGF-1), transgenic mice containing IGF-1 Receptor (IGF-1R) cDNA fused to metallothionein promoter were produced by DNA microinjection into the pronucleus of mouse zygote. Three founders were produced with transgenic mice containing IGF-1R gene. Transgenic mice lines contained approximately $4{\sim}20$ copies of transgenes per cell and transmission of this gene into the progeny with Mendelian manner were determined. The founder mice were mated with normal mice to produce $F_1$ mice and then $F_2$ mice. Transmission rates of IGF-1R transgene in the progeny mice were $25{\sim}60%$ in $F_1$ generation and $40{\sim}50%$ in $F_2$ generation. The mRNA expression of IGF-1R transgene in liver was analyzed using RT-PCR for IGF-1R gene in liver. When body weights of transgenic pups were measured during 4, 10 and 14 weeks after birth, IGF-1R transgenic mice grew faster than non transgenic littermates. This study indicated that growth regulation by IGF-1 signaling through IGF-1R can be elucidated using IGF-1R transgenic mice.

• 한국산학기술학회논문지
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• 제21권5호
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• pp.240-248
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• 2020

지난 20여 년 간 뇌 과학은 기능성 자기공명영상(fMRI) 등 새로운 기술에 힘입어 크게 발전했으며, 우리는 인간의 본성과 행동 변화에 대해 더욱 정확한 지식을 얻게 됐다. 이 같은 지식은 경영 분야에도 활발하게 응용되고 있다. 이 연구는 마이다스아이티의 사례를 통해 뇌과학을 경영에 어떻게 접목할 수 있는지 통찰을 얻는 것이 목적이다. 건설 소프트웨어 회사인 이 회사는 별도 조직을 두어 뇌 과학을 연구하고 있으며, 뇌 과학에 기반한 인사 정책을 만들어 시행하고 있다. 창업자 이형우 사장은 인본주의 경영 철학을 갖고 있으며, 뇌 과학 연구는 그 철학을 뒷받침한다. 연구 방법으로 사례연구 방법을 채택하였으며, 인터뷰와 직접 관찰, 참여 관찰, 문서 정보 등의 절차를 수행했다. 이 회사의 인적자원 관리 시스템은 뇌과학 연구가 응집된 'SCARF'라는 뇌과학 모델로 설명이 가능하다. 이 회사는 이 모델이 제시하는 것처럼 일터에서 지위감(Status), 확실성(Certainty), 자율감(Autonomy), 관계성(Relatedness), 공정성(Fariness) 위협을 줄임으로써 구성원의 신뢰와 만족도를 제고했고, 이는 창의적이고 고성과 조직이 되는 발판이 되었다.

• 한국도서관정보학회지
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• 제20권
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• pp.241-284
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• 1993

Through the restoration, we can find that the register of houses, inhabitants and their ancestry, which is in the Sunsung Kim family registers, was fundamentally the census register of Kim Roe including more ancestry transformed into the lists of house, inhabitants and their ancestry of Kim Roe, Kim Bang-Seek, Kim Sung-Sae and Kim Hee-Bo. And we can find also the original forms were considerably damaged. That is, in the course of transformation, considerable parts of the contents of ancestry were not needed, so they were removed, the recordings of brotherandsisters, sons and their servants were also removed. By comparing of the restored census register of Kim Roe and the Sunsung Kim family registers, we can know the fact that when they published the family registers, the contents of the founder to the ninth descendants were totally depended on the census register of Kim Roe. The Census Register of King Taejo of Choson Dynasty(National Treasure No. 131) which has been recorded almost same periods as the census register of Kim Roe was remained as an original state. Therefore, it was greatly helpful for restoring the census register of Kim Roe. There were few materials which we can know the way of ordinary life in Koryo period. But through the census register of Kim Roe and the census Register of King Taejo of Choson Dynasty, we have a glimpse of their life history. Nevertheless we can find some demerits in the census register of Kim Roe as followers : First, it is not an original but a transformed one, while the Census Register of King Taejo of Choson Dynasty is first materials. Second, it was recorded the only one family. Finally, it was omitted the parts of his brotherandsisters, children and servants who lived with their master. According to these demerits its worth of materials for history will be descended more or less. Therefore, when we use this material, we should treat it more considerably.

• 대한한의학원전학회지
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• 제20권2호
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• pp.137-145
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• 2007

Pulse diagnosis that distinguishes internal injury from external injury by comparing the left and right of the chon pulse was formed in the process of Naegyoung's pulsation theory of ST9 and LU9 being assimilated into diagnostic method by taking chon pulse. The founder of school of internal injury, I Dong-won, expanded the horizon for this method to be widely used in clinical practice by especially explaining the specific application and theoretical background. According to him, pulse at ST9 which means chon pulse at the left hand, is bigger than the chon pulse at the right hand, it reflects external injury. Bigger "entrance pulse", a chon pulse at the right hand means internal injury. The reason is the left side of the body is a path for Yanggi so it controls the exterior part and the right side of the body is a path for Eumgi to descend so it controls the interior part. Internal injury develops as the spleen and stomach get injured. If the spleen and stomach is damaged essence derived from food cannot ascend to the stomach and will flow back to the lower part. As a result, fire of Eum type formed at the lower part will shoot up to the upper part and manifests external injury-like exterior syndromes. In this case, evidence distinguishing between internal and external injury is the fact that right hand pulse is bigger than the left hand. The important reason for distinguishing between internal and external injury is because when treating external injury caused by excess syndrome, pathogenic Gi should be dispelled. However, treating internal injury cased by deficiency syndrome, requires promoting the primordial Gi.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2675-2679
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• 2012

Introduction: Annually a considerable number of people die because of breast cancer, a common disease among women also in Iran. Identifying risk factors and susceptible people can lead to prevention or at least early diagnosis. Among susceptibility risks, 5-10% of patients have a family history predisposing factor which can influence the risk of incidence among the family. Having a registry program can be a more practical way to screen high risk families for preventive planning. Method: Based on inclusion criteria, a questionnaire was prepared and after a pilot study on a small number of patients, actual data were collected on 400 patients and processed in SPSS 16.0. Results: Totally, 28.2%of the patients were younger than 40 years old and 36.8% had the included criteria for familial breast cancer (FBC). 102 patient's samples could be compared for receptor presentation. Similar to other studies, the number of triple negative breast cancers increased as the age decreased. Conclusion: The high percentage of patients with FBC among 400 cases in this study demonstrates that in order to design an infrastructural diagnostic protocol and screening of patients with FBC, a precise survey related to frequency and founder mutations of FBC is needed nationwide.

• 한국콘텐츠학회논문지
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• 제14권7호
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• pp.226-241
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• 2014

본 연구는 중년직장인의 퇴직 후 창업효능감과 창업의도에 영향을 미치는 요인에 관한 연구로서, 이들 종속변수들과 창업자 개인의 혁신성, 성취욕구, 위험감수성향, 통제소재, 그리고 가족지지와의 관계를 분석하였다. 이를 위하여 서울 및 경기지역에 근무하는 중년직장인들을 대상으로 설문조사를 하였으며, 총 346부의 자료를 사용하였다. 연구결과는 다음과 같다. 첫째, 개인의 성취욕구, 위험감수성향 그리고 통제소재가 강할수록 창업효능감이 높고 둘째, 혁신성, 성취욕구 그리고 위험감수성향이 높을수록 창업의도가 높은 것으로 나타났으며 셋째, 가족지지는 성취욕구와 창업효능감 간에 조절역할을 하는 것으로 나타났다. 마지막으로 인구사회학적 특성에 따라 개인특성, 가족지지, 창업효능감 그리고 창업의도는 부분적으로 차이가 났다.

• 한국콘텐츠학회논문지
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• 제15권11호
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• pp.422-432
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• 2015

경제의 저성장이 지속됨에 따라 고용창출과 경제 활성화를 위해 정부는 중소기업을 지원하기 위한 다양한 제도를 시행하고 있으며 다양한 형태의 지원을 통해 창업을 더욱 활성화 시키고 있다. 이러한 창업지원제도는 창업 활성화에 일정 부분 기여하고 있다고 볼 수 있으나, 본 연구에서는 실증적인 연구룰 통하여 창업지원에 대해 다양하고 구체화된 대안을 제시하고 창업의지를 장려하는 효과를 기대해 보고자 한다. 본 연구는 선행연구를 바탕으로 창업의지에 미치는 영향을 조세지원제도, 금융지원제도, 기술지원제도, 경영지원제도, 창업인프라지원제도의 5가지로 설정하고 독립변수가 창업의지에 미치는 영향을 각각 가설로 설정하여 실증 연구하였다. 이를 위해 본 연구에서는 변수들을 도출하여 그 변수들 간의 관계를 검증하기 위한 변수의 측정과 문항으로 구성된 설문지를 이용하였으며 측정된 자료의 통계처리에 의거하여 검증하는 실증적 연구방법을 수행하였다. 연구가설을 검증하기 위하여 요인분석, 상관관계분석, 경로분석 등의 통계적 방법을 적용하였으며. 경로분석결과는 조세지원제도, 금융지원제도가 창업의지에 영향을 미치는 것으로 나타났다.