• Microbiology and Biotechnology Letters
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• v.13 no.1
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• pp.33-39
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• 1985

The effects of initial concentration, flow rate, and recycle ratio on the removal efficiency of phenol were studied in a tapered fluidized bed reactor packed with activated carbon which was attached with Candida tropicalis. The optimum conditions of Candida tropicalis were showed that pH was 7.0 and temperature was $30^{\circ}C$, and the specific growth rate of Candida tropicalis was satisfied with the Monod equation up to 500 mg/L of phenol, and beyond it the inhibition of substrate was found. According to the increases of initial concentration and flow rate, the removal efficiency was decreased, as the recycle ratio was increased, the removal efficiency was increased. In the case of flow rate of 10mL/sec and the recycle ratio of 2, the removal efficiency was 90% above for the all of initial concentration. The removal rate of phenol was the first order reaction in this system, and the rate equation of reaction was as follows.

• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.273-278
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• 1985

A carrier-supported mycelial growth of Penicillium chrysogenum was applied to penicillin fermentation system. Among various materials tested, celite was found to be most effective for both spore adsorption and bioparticle development. Hyphal growth through pore matrices of the material showed strong anchorages and provided highly stable biofilm growths. When 5-10％ celite was employed, both cell growth and penicillin production were observed to increase significantly comparing to the dispersed filamentous growth. Specific productivity of penicillin, however. was found to be kept almost constant at a value of 1,900 unit/g cell/hr. A semicontinuous fermentation in a fluidized-bed reactor. using the tarrier-supported biofilm growth, was conducted successfully although free mycelia appeared in the late phase of the fermentation made the reactor operation difficult. Control of the size of bioparticles was considered as a major operating factor to maintain the reactor productivity at a desired level.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.2
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• pp.331-340
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• 2000

The existing two-phase biological fluidized bed has some problems such as limit of oxygen transfer and blockade of fluidized distributor. In this study, three-phase swirl flow biological fluidized bed has designed to solve the problems and to investigate its running characteristics. TOC of influent synthetic wastewater was approximately $70mg/{\ell}$. HRT of reactor was 1.6 hours. Mean particle size of sand, as packing media, was 0.397mm and packing volume was varied from $200m{\ell}/{\ell}$ to $600m{\ell}/{\ell}$ by stages in the bed. The amount of biomass and effluent water quality was throughly investigated in the bed. Showing experiment results from the above conditions, it was possible to solve the problems of existing fluidized bed and to keep DO of $3mg/{\ell}$ or more. And it was also TOC removal rate of 91 to 94 %, MLVSS of 2,360 to $3,860mg/{\ell}$, MLVSS per g-media of 8.4 to 17.3 mg/g, F/M ratio of 0.59 to $1.04kg-TOC/kg-MLVSS{\cdot}day$, biofilm thickness of $35{\sim}71{\mu}m$ and sludge productivity of 1.03 to $2.35kg-SS/m^3{\cdot}day$. Optimal conditions in this experimental were as follows.; those were biofilm thickness of approximately $54{\mu}m$. MLVSS per g-media of 13 mg and media packing volume of 350 to $400m{\ell}/{\ell}$ when F/M ratio was low, treatment efficiency was high and sludge productivity was low. Showing the media with optics microscope in this optimal condition, attached microbes such as Epistylis sp. were observed. From SEM photographs, it showed that Coccus adhere to and grow on the media surface.

• Journal of Korean Society of Water and Wastewater
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• v.12 no.4
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• pp.59-69
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• 1998

Biological treatment systems generally can be divided into two main classes of a suspended sludge process and attached one like a fluidized bed reactor. These process are considered to bring remarkable change in species composition of microorganisms, due to difference of a state of biofilm, a concentration and diffusion velocity of dissolved oxygen, a concentration and diffusion velocity of substance or poisonous matter. The change of species composition bring different treatment result for influence factors like F/M ratio, DO concentration, pH or poisonous matter. This study is to investigate the reaction characteristics of both microorganisms, namely, a suspended sludge and attached sludge, through the changes of pH, temperature and substance concentration.