• Journal of Environmental Health Sciences
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• v.23 no.3
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• pp.80-84
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• 1997

This study was conducted from April 11, 1996 to May 12, 1996 in Taegu and its vicinities. The purpose of this study was to determine storage conditions of pasteurized fluid milk at retail food establishments. The results are summarized as follows 1. 12(9.5%) out of 126 retail food establishments displayed out dated fluid milk. In a county, more out dated fluid milk(18.2%) were found in retail food establishments in town area eup than those (2.5%) in township area myeon.(P<0.05) 2. 47(37.3%) retail food establishments stored fluid milk below 10$\circ$C, and 79(62.7%) stored them above 10$\circ$C. 3. Only 18(14.3%) refrigerators out of 126 had thermometers to check temperature of a refrigerator. 4. Coliform bacteria were isolated from 3(2.4%) out of 126 fluid milk samples.

• Animal Bioscience
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• v.34 no.2
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• pp.213-222
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• 2021

Objective: The metabolites that constitute the rumen fluid and milk in dairy cattle were analyzed using proton nuclear magnetic resonance (1H-NMR) spectroscopy and compared with the results obtain for other dairy cattle herds worldwide. The aim was to provide basic dataset for facilitating research on metabolites in rumen fluid and milk. Methods: Six dairy cattle were used in this study. Rumen fluid was collected using a stomach tube, and milk was collected using a pipeline milking system. The metabolites were determined by 1H-NMR spectroscopy, and the obtained data were statistically analyzed by principal component analysis, partial least squares discriminant analysis, variable importance in projection scores, and metabolic pathway data using Metaboanalyst 4.0. Results: The total numbers of metabolites in rumen fluid and milk were measured to be 186 and 184, and quantified as 72 and 109, respectively. Organic acid and carbohydrate metabolites exhibited the highest concentrations in rumen fluid and milk, respectively. Some metabolites that have been associated with metabolic diseases (acidosis and ketosis) in cows were identified in rumen fluid, and metabolites associated with ketosis, somatic cell production, and coagulation properties were identified in milk. Conclusion: The metabolites measured in rumen fluid and milk could potentially be used to detect metabolic diseases and evaluate milk quality. The results could also be useful for metabolomic research on the biofluids of ruminants in Korea, while facilitating their metabolic research.

• East Asian Economic Review
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• v.21 no.3
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• pp.259-274
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• 2017

This study estimates the random utility function of fluid milk using 1,165 survey responses in Laos. It finds that both products' attributes and individual characteristics affect consumers' preference for the milk and the hypothetical brand of Laos-Korea has a potential compared to four real dairy products. Results also show that calories have a positive relationship with consumer's preference while the price and fat content have a negative one. The decision for choosing each brand is significantly affected by individual characteristics such as gender, age, whether or not respondents live with their children, the level of education, income, the frequency of purchasing milk per week, and the region where they live. The preference for five brands appears in the order of Foremost, Nabong, Thai-Danish, Meiji, and Lao-Korea, and probabilities of purchasing each brand at the mean level are 30.9%, 17.48%, 21.48%, 15.0% and 10.39%, respectively. Nabong that was Lao national milk brand still has a significant market power even though it was closed in 2008. The policies to promote milk industry by implementing its national milk brand again would be more effective if it focuses on the young generation, female consumers, families with children, quality of dairy products, and Vientiane capital areas.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.1
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• pp.25-29
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• 1991

Performance and production of twenty lactating Holstein cows fed diets containing either soybean meal or fish meal as the primary protein source were compared in a continuous or split feeding scheme. At 1 wk prepartum four groups of five animals were placed on each experimental diet. Animals assigned to the continuous feeding scheme were continued on these diets for 10-wk postpartum. At 4 wk postpartum, the diets for the groups assigned to switching protein sources were changed. These treatments were continued for another 6 wk. Milk production and dietary intakes were recorded daily. Milk constituents were measured every 2 d. Cows weights, rumen fluid samples and jugular blood samples were collected weekly. Data showed no effect of early lactation diet on cow performance or milk characteristics. Overall, compared to the soybean meal diet, the fish meal diet lowered the milk fat percent and increased production of milk per unit of dry matter ingested. No differences were observed for volatile fatty acid content of rumen fluid, blood mineral content, milk protein, somatic cell count, 4%-fat corrected milk, dry matter intake, or body weight.

• Asian-Australasian Journal of Animal Sciences
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• v.2 no.3
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• pp.418-420
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• 1989

• Korean Journal of Agricultural Science
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• v.16 no.1
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• pp.71-83
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• 1989

The purpose of this research are to forecast the demand and supply of milk in Korea, and to obtain information for attitudes affecting milk consumption, which is necessary to make a plan for increasing milk consumption in Korea. The estimation of the milk demand and production was made by the multiplicative decomposition method and the statistical function. Data on consumer were collected from 737 students who were attending primary school, middle school and university in Daejeon during the period of July 11 to July 21, 1988. The results obtained are as follows; 1. The prediction results showed that the production for milk will over supply 21,900 tons in 1,990, 70,800 tons in 1,995 by the multiplicative decomposition method and 45,400 tons in 1990, -51,500 tons in 1995 by the statistical function. 2. It was found that almost all the students awared milk as essential food-stuff of common food stuff for the Koreans. 3. Quite a few students were apt to believe that milk processors added water into fluid milk. 4. Most students showed obtaining information about the nutritional value of milk by school education and advertising of TV, Radio, and Printed media. 5. However, it was found that the advertising by TV, Radio, and Printed media did hardly give to consumers influences on the choice of a particular milk brand. Accordingly, the conclusions are as follows; 1. Need to provide consumers with well planned education programs on the nutritional value of milk. 2. Heavy brand advertising for fluid milk may mislead the understanding of consumer, since city milk is not much differentiated in Korea. Therefore the milk processors should put more efforts in generic milk promotion by reducing brand advertizement. 3. The milk processors should provide major portion of financing for generic milk promotion program.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.9
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• pp.1262-1270
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• 2008

The objective of this study was to investigate the effect of dietary supplementation with calcium salts of soybean oil fatty acids (CaSO) and linseed oil fatty acids (CaLO) on c9,t11-CLA production in ruminal fluid and milk fat from Holstein dairy cows. Rumen fermentation, lactational performances and fatty acid profiles in ruminal fluid and milk fat were also investigated. Twenty multiparous Holstein dairy cows were allotted randomly into two groups consisting of ten cows in each group according to calving date and average milk yield. The first group of cows was fed a control (without calcium salts) diet and a treatment as 1.0% of CaSO (on DM basis) for 30 days in each period. In the second group, cows were fed the same control diet and 1.0% of CaLO as a treatment in the same manner. The forage: concentrate ratio was 52:48, and diets were formulated to contain 17% crude protein (DM basis) for both groups. Ruminal pH, protozoal numbers and the concentration of total volatile fatty acids were unchanged, however, the ruminal ammonia-N decreased by feeding CaSO or CaLO treatment compared to the control diet. The vaccenic acid (trans-11 C18:1; VA) in rumen fluid increased (p<0.01) by 169% and 153%, and the c9,t11-CLA content of rumen fluid increased (p<0.01) by 214% and 210% in the CaSO and CaLO treatments, respectively, compared to the control diet. In milk fatty acids, the VA content increased by 130% and 132% in the evening and morning milking times, respectively, and the c9,t11-CLA content increased by 125% in both milking times for the CaSO supplementation than that of control diet. In the case of CaLO supplementation, the VA increased by 117% and 114%, and the c9,t11-CLA increased by 96% and 94% in the evening and morning milking times, respectively, compared to the control diet. The contents of VA and c9,t11-CLA of milk fatty acids were numerically higher in the evening milking time compared to the morning milking time for control and both treatments. Finally, these results indicated that the supplementation of CaSO or CaLO treatment increased the VA and the c9,t11-CLA in both ruminal fluid and milk fat of Holstein dairy cows.

• Archives of Pharmacal Research
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• v.26 no.5
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• pp.426-431
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• 2003

This study was carried out to investigate the addition of water-soluble isoflavone into milk by means of microencapsulation technique. The yield of microencapsulation, sensory attributes, and capsule stability of water-soluble isoflavone microcapsules in milk were measured. Coating materials used was polyglycerol monostearate (PGMS), and core material was water-soluble isoflavone. The encapsulation yield of water-soluble isoflavone with PGMS was 67.2% when the ratio of coating material to core material was 15 : 1. The rate of water-soluble isoflavone release from capsules was 18, 19, and 25% when stored at 4,20, and $30^{\circ}C$ for 12 days in milk, respectively. In sensory evaluation, beany flavor and color of microencapsuled water-soluble isoflavone added milk were significantly different from uncapsuled water-soluble isoflavone added milk, however, bitterness was not significantly different. In vitro study, micro-capsules of water-soluble isoflavone in simulated gastric fluid with the range of 3 to 6 pHs were released 3.0∼15.0%, however, the capsules in simulated intestinal fluid with pH 7 were released 95.7% for 40 min incubation time. In conclusion, this study provided that PGMS as coating materials was suitable for the microencapsulation of water-soluble isoflavone, and the capsule containing milk was almost not affected with sensory attribute.

• Journal of Food Hygiene and Safety
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• v.5 no.1
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• pp.35-40
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• 1990

As a link in the studies on the extracellular polysaccharide by lactic acid bacteria, the experiment was conducted to investigate the viscosity variations and rheological properties of 10% reconstituted skim milk and 12% reconstituted whole milk, respectively. 1. 10% reconstituted skim milk cultured by Str. thrennophilus 510 showed strong flow property of pseudoplastic fluid depending upon the production of exopolysaccharide. And the viscosity reached the highest value within 14% concentration. 2. 12% reconstituted whole milk cultured by lactic acid bacteria indicated flow property of pseudoplastic fluid. But there was a big difference in the viscosity as compared with 10% skim milk. 3. The maximum consistency index (k) and the minimum flow behavior index (n) of the fermented milk by Str. thermophilus 510 were 43 and 0.09, respectively. They were 35 and 0.09, in case of Lb. bulgaricus.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.1
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• pp.79-90
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• 2020

Objective: In the present study, an liquid chromatography/mass spectrometry (LC/MS) metabolomics approach was performed to investigate potential biomarkers of milk production in high- and low-milk-yield dairy cows and to establish correlations among rumen fluid metabolites. Methods: Sixteen lactating dairy cows with similar parity and days in milk were divided into high-yield (HY) and low-yield (LY) groups based on milk yield. On day 21, rumen fluid metabolites were quantified applying LC/MS. Results: The principal component analysis and orthogonal correction partial least squares discriminant analysis showed significantly separated clusters of the ruminal metabolite profiles of HY and LY groups. Compared with HY group, a total of 24 ruminal metabolites were significantly greater in LY group, such as 3-hydroxyanthranilic acid, carboxylic acids, carboxylic acid derivatives (L-isoleucine, L-valine, L-tyrosine, etc.), diazines (uracil, thymine, cytosine), and palmitic acid, while the concentrations of 30 metabolites were dramatically decreased in LY group compared to HY group, included gentisic acid, caprylic acid, and myristic acid. The metabolite enrichment analysis indicated that protein digestion and absorption, ABC transporters and unsaturated fatty acid biosynthesis were significantly different between the two groups. Correlation analysis between the ruminal microbiome and metabolites revealed that certain typical metabolites were exceedingly associated with definite ruminal bacteria; Firmicutes, Actinobacteria, and Synergistetes phyla were highly correlated with most metabolites. Conclusion: These findings revealed that the ruminal metabolite profiles were significantly different between HY and LY groups, and these results may provide novel insights to evaluate biomarkers for a better feed digestion and may reveal the potential mechanism underlying the difference in milk yield in dairy cows.