• Journal of the korean academy of Pediatric Dentistry
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• v.26 no.2
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• pp.399-415
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• 1999

From bacteria to mammalian cells, one of the most important mediators of intracellular signal transduction mechanisms which regulate a variety of intracellular processes is free calcium. In salivary acinar cells, elevation of intracellular calcium concentration ($[Ca^{2+}]_i$) is essential for the salivary secretion induced by parasympathetic stimulation. However, in addition to $[Ca^{2+}]_i$, gap junctions which couple individual cells electrically and chemically have also been reported to regulate enzyme secretion in pancreatic acinar cells. Since the plasma membrane of salivary acinar cells has a high density of gap junctions, and these cells are electrically and chemically coupled with each other, gap junctions may modulate the secretory function of salivary glands. In this respect, I planned to investigate the role of gap junctions in the modulation of salivary secretion and $[Ca^{2+}]_i$, using mandibular salivary glands of rats. In order to measure the salivary flow rate, fluid was collected from the cannulated duct of the isolated perfused rat mandibular glands at 2 min intervals. $[Ca^{2+}]_i$, was measured from the cells loaded with fura-2 by spectrofluorometry. The results obtained were as follows: 1. CCh-induced salivary secretion was reversibly inhibited by 1 mM octanol, a gap junction blocker. 2. CCh-induced increase in $[Ca^{2+}]_i$, was also reversed by the application of 1 mM octanol. 3. Octanol did not block the initial increase in $[Ca^{2+}]_i$ caused by CCh, which suggested that the reduction of $[Ca^{2+}]_i$, caused by gap junction blockade was not resulted from the inhibition of $Ca^{2+}$ release from intracellular $Ca^{2+}$ stores. 4. Addition of octanol during stimulation with $1{\mu}M$ thapsigargin, a potent microsomal ATPase inhibitor, reduced $[Ca^{2+}]_i$, to the basal level. This suggested that inhibition of gap junction permeability closed plasma membrane $Ca^{2+}$ channels. 5. 2,5-di-tert-butyl-1,4-benzohydroquinone (TBQ) generated $[Ca^{2+}]_i$ oscillations resulting from periodic influx of $Ca^{2+}$ via plasma membrane. The TBQ-induced $[Ca^{2+}]_i$ oscillations were stopped by the application of 1mM octanol which implicated that gap junctions modulate the permeability of plasma membrane $Ca^{2+}$ channels. 6. Glycyrrhetinic acid, another well known gap junction blocker, also inhibited CCh-induced salivary secretion from rat mandibular glands. These results suggested that gap junctions play an important role in the modulation of fluid secretion from the rat mandibular glands and this was probably due to the inhibition of $Ca^{2+}$ influx through the plasma membrane $Ca^{2+}$ channels.

• Journal of the Korean Society for Marine Environment & Energy
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• v.4 no.4
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• pp.21-31
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• 2001

When an oil-spilling accident occurs at sea, it is of the primary importance to predict the amount of oil leakage for the swift response and decision-making. The simplest method of oil-leakage estimation is based on the hydrostatic pressure balance between oil inside the tank and seawater outside of leakage hole, that is the so-called Torricelli equilibrium relation. However, there exists discrepancy between the reality and the Torricelli relation, since the latter is obtained from the quasi-steady treatment of Bernoulli equation ignoring viscous friction. A preliminary experiment has been performed to find out the oil-leaking speed and shape. Soy-bean oil inside the inner tank was ejected into water of the outer tank through four different leakage holes to record the amount of oil leakage. Furthermore, a CFD (Computational Fluid Dynamics) method was utilized to simulate the experimental situation. The Wavier-Stokes equations were solved for two-density flow of oil and water. VOF method was employed to capture the shape of their interface. It is found that the oil-leaking speed varies due to the frictional resistance of the leakage hole passage dependent on its aspect ratio. The Torricelli factor relating the speed predicted by using the hydrostatic balance and the real leakage speed is assessed. For the present experimental setup, Torricelli factors were in the range of 35%~55% depending on the aspect ratio of leakage holes. On the other hand, CFD results predicted that Torricelli factor could be 52% regardless of the aspect ratio of the leakage holes, when the frictional resistance of leakage hole passage was neglected.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.12
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• pp.446-452
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• 2016

Recently, heat resistant super alloys (which are wear-resistant, corrosion-resistant, and heat-resistant), have been used as the basic structural material in offshore and petrochemical plants. On the other hand, making valves from very expensive, high heat-resistant alloys increases the production cost and decreases its market competitiveness. To solve these problems, the technique of overlaying only those that flow on the fluid has been used as an effective method. Nevertheless, because the former technique of overlaying the ball is performed manually, it takes too much time and perfect welding is difficult to perform. To solve this problem, this study developed a robot automation system that can make uniformly overlay welding of the ball for ball-valves. The system consists of a 6-axis welding robot with a welding torch and additional 2 axes for the rotation of positioner, the controller, and a robot path OLP (Off-Line Programming). The CAD drawing data was entered in the Off-line program to obtain the robot teaching point and drive source. Overlay welding paths were implemented using Matlab. Through an automated overlaying system that implemented the OLP, the productivity rose 2.58 times, as the amount of time required for work decreased from 88 hours to 41 hours.

• Journal of radiological science and technology
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• v.39 no.4
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• pp.623-630
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• 2016

Liver tissue damage by a radiation exposure caused a jaundice and ascitic fluid e form harden atrophy. The reason for this lies in morphological damage of a liver cells. This study tried that observe damage mechanism of the cell organelles. It was especially observed mitochondria, endoplasmic reticulum and nuclear membrane associated with energy metabolizable. also, This study had with a radio-protector development research at the same time. Radio-protector was used to alliin that has an blood flow increase. Cell observation make used of transmission electron microscope(TEM). The result of an experiment, 7Gy of whole body irradiation was caused an inflammation in cell organelles and hypertrophy of the nucleus membrane. After 20 days, The hepatocyte has been observed in a damaged membrane on peroxisome, mitochondria and vacuole of the cell organelles. After 30 days, The hepatocyte has been observed in disconnected ribosomes on a rough endoplasmic reticulum. There was looked a giant lipoblast. There was clearly normal observed a mitochondria and nucleus membrane in the hepatocyte after alliin injection. aslo, It was no damaged the nucleus membrane. therefore, It was identified portion a radio-protector effect from alliin.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.12 no.2
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• pp.121-133
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• 2014

If the spent fuels or the high-level radioactive wastes can be disposed of in the depth of 3~5 km and more stable rock formation, it has several advantages. For example, (1)significant fluid flow through basement rock is prevented, in part, by low permeability, poorly connected transport pathways, and (2)overburden self-sealing. (3)Deep fluids also resist vertical movement because they are density stratified and reducing conditions will sharply limit solubility of most dose-critical radionuclides at the depth. Finally, (4) high ionic strengths of deep fluids will prevent colloidal transport. Therefore, as an alternative disposal concept to the deep geological disposal concept(DGD), very deep borehole disposal(DBD) technology is under consideration in number of countries in terms of its outstanding safety and cost effectiveness. In this paper, for the preliminary applicability analyses of the DBD system for the spent fuels or high level wastes, the DBD concepts which have been developed by some countries according to the rapid advance in the development of drilling technology were reviewed. To do this, the general concept of DBD system was checked and the study cases of foreign countries were described and analyzed. These results will be used as an input for the analyses of applicability for DBD in Korea.

• Economic and Environmental Geology
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• v.49 no.1
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• pp.31-41
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• 2016

A program code was developed to calculate block hydraulic conductivity of the 2-D DFN(discrete fracture network) system based on equivalent pipe network, and implemented to examine the effect of joint orientation distribution on the hydraulic characteristics of fractured rock masses through numerical experiments. A rock block of size $32m{\times}32m$ was used to generate the DFN systems using two joint sets with fixed input parameters of joint frequency and gamma distributed joint size, and various normal distributed joint trend. DFN blocks of size $20m{\times}20m$ were selected from center of the $32m{\times}32m$ blocks to avoid boundary effect. Twelve fluid flow directions were chosen every $30^{\circ}$ starting at $0^{\circ}$. The directional block conductivity including the theoretical block conductivity, principal conductivity tensor and average block conductivity were estimated for generated 180 2-D DFN blocks. The effect of joint orientation distribution on block hydraulic conductivity and chance for the equivalent continuum behavior of the 2-D DFN system were found to increase with the decrease of mean intersection angle of the two joint sets. The effect of variability of joint orientation on block hydraulic conductivity could not be ignored for the DFN having low intersection angle between two joint sets.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.27 no.4
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• pp.286-292
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• 1991

The authors propose to use the vortex generators in order to improve of the efficiency for the cambered otter boards. The equipments and testing method of this model test was the same as the previous report. This study was tested for 6 models such as the single cambered, the V-shaped cambered and the slotted cambered otter board without and with vortex generators. The results obtained are as follows: \circled1 C sub(L) of the single cambered model otter board with vortex generators was increased about 10% in comparison with that of model without vortex generators, C sub(D) decreased 2%, and L/D increased 5~20%. \circled2 L/D of the V-shaped cambered model otter board with vortex generators was increased 10~20% in comparison with that of model without vortex generators. \circled3 C sub(L) of the two slotted cambered model otter board with vortex generators was increased about 20% within an angle of attack 25$^{\circ}$ in comparison with that of without vortex generators, C sub(D) increased 5~20%, and L/D was higher than prototype within an angle of attack 20$^{\circ}$. \circled4 The separation point of the model otter boards with vortex generators was removed back ward a little in comparison with that of the model without vortex generators. \circled5 Flow speed difference of the back side to the front side of model otter boards with vortex generators was increased a little in comparison with that of the models without vortex generators. \circled6 The size of separation zone in case of the model otter boards with vortex generators was decreased about 10% in comparison with that of the models without vortex generators.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.23-28
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• 2019

The structural integrity of a surface metal coating was evaluated through numerical results to improve the efficiency and reduce the damage caused by cavitation in ships and marine plants. The goal was to ensure structural strength and performance, even if the thickness of the wing is reduced to reduce the weight of the material and surface coating. Analytical methods were used for four models: a non-coating model, one with the same thickness after coating, one with a thickness reduction of 3% after coating, and one with thickness reduction of 5% after coating. With a thickness reduction of 5% after coating, the stress was increased to 12%, and the safety factor was 0.99%, so the structural integrity was insufficient. However, a better material or a thicker coating could allow a sufficient safety factor to be secured. The structural integrity was improved by the coating, and even when the weight was reduced up to 5%, the structural integrity could be sufficiently secured due to the coating effect.

• Journal of the Institute of Convergence Signal Processing
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• v.23 no.4
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• pp.228-233
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• 2022

This study is an experimental study on how to use phase change materials(PCM) to generate electrical energy for long-term operation of underwater unmanned vehicles. The electrical energy generation method is a volume change and a pressure change that occur as a phase change material changes to a solid or liquid state according to temperature, and the change in pressure creates a flow of fluid to create electrical energy. Polyethylene glycol was used as a phase change material considering the temperature of the ocean. In addition, an electrical energy generating device that converts volume change into pressure at low temperature (1℃~2℃) in solid state and high temperature (21℃~25℃) in liquid state was fabricated. As a result of the experiment, the pressure change according to the phase change rapidly changed between 1 hour and 2 hours, and maintained a pressure of about 24MPa after 4 hours. Through this, it was confirmed that it can be used as a power source for underwater unmanned vehicles using phase change materials and temperature differences. In addition, it was found that a more improved design should be made in order to apply the phase change material to an underwater unmanned vehicle.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.1B
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• pp.95-109
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• 2008

We numerically analyzed the nonlinear shoaling, a plunging breaker and its accompanying energetic suspension of sediment at a bed, and a redistribution of suspended sediments by a down rush of preceding waves and the following plunger using SPH with a Gaussian kernel function, Lagrangian Dynamic Smagorinsky model (LDS), Van Rijn's pick up function. In that process, we came to the conclusion that the conventional model for the tractive force at a bottom like a quadratic law can not accurately describe the rapidly accelerating flow over a swash zone, and propose new methodology to accurately estimate the bottom tractive force. Using newly proposed wave model in this study, we can successfully duplicate severely deformed water surface profile, free falling water particles, a queuing splash after the landing of water particles on the free surface and a wave finger due to the structured vortex on a rear side of wave crest (Narayanaswamy and Dalrymple, 2002), a circulation of suspended sediments over a swash zone, net transfer of sediments clouds suspended over a swash zone toward the offshore, which so far have been regarded very difficult features to mimic in the computational fluid mechanics.