• Title/Summary/Keyword: fluid and flow

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Evaluation of Sperm Sex-Sorting Method using Flow Cytometry in Hanwoo (Korean Native Cattle)

  • Yoo, Han-Jun;Lee, Kyung-Jin;Lee, Yong-Seung;Lee, Chang-Woo;Park, Joung-Jun;Cheong, Hee-Tae;Yang, Boo-Keun;Park, Choon-Keun
    • Journal of Embryo Transfer
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    • v.27 no.1
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    • pp.37-43
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    • 2012
  • This study evaluated a method of sorting X and Y chromosomes based on size using the forward angle light scatter related refractive index (FSC) of a flow cytometer. Hanwoo bulls sperm were separated to X and Y chromosomes by the parameters of FSC or Hoechst 33342 intensity. As a result, using monitor program linked flow cytometry during sorting processing, the purities were $97{\pm}0.57$ or $96{\pm}0.67%$ for the X-fraction and $96{\pm}0.33$ or $97{\pm}1.33%$ for the Y-fraction in the two sperm sorting methods. There were no differences in the X and Y ratios (X and Y %) between the sperm sorting methods based on FSC or DNA content. The proportions of female and male embryos used for in vitro fertilization and development were $66.03{\pm}3.31$ or $69.37{\pm}1.41%$, and $70.56{\pm}2.42$ or $56.11{\pm}3.09%$ when sperm were processed using the sex sorting method by FSC or Hoechst 33342. In conclusion, further study is needed to determine the optimum procedure and improve the nozzle to enhancing sorting accuracy or efficiency. Also, the findings of this study do not negate the possibility that the difference method of sperm sorting cannot use a UV laser beam.

Mechanism on suppression in vortex-induced vibration of bridge deck with long projecting slab with countermeasures

  • Zhou, Zhiyong;Yang, Ting;Ding, Quanshun;Ge, Yaojun
    • Wind and Structures
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    • v.20 no.5
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    • pp.643-660
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    • 2015
  • The wind tunnel test of large-scale sectional model and computational fluid dynamics (CFD) are employed for the purpose of studying the aerodynamic appendices and mechanism on suppression for the vortex-induced vibration (VIV). This paper takes the HongKong-Zhuhai-Macao Bridge as an example to conduct the wind tunnel test of large-scale sectional model. The results of wind tunnel test show that it is the crash barrier that induces the vertical VIV. CFD numerical simulation results show that the distance between the curb and crash barrier is not long enough to accelerate the flow velocity between them, resulting in an approximate stagnation region forming behind those two, where the continuous vortex-shedding occurs, giving rise to the vertical VIV in the end. According to the above, 3 types of wind fairing (trapezoidal, airfoil and smaller airfoil) are proposed to accelerate the flow velocity between the crash barrier and curb in order to avoid the continuous vortex-shedding. Both of the CFD numerical simulation and the velocity field measurement show that the flow velocity of all the measuring points in case of the section with airfoil wind fairing, can be increased greatly compared to the results of original section, and the energy is reduced considerably at the natural frequency, indicating that the wind fairing do accelerate the flow velocity behind the crash barrier. Wind tunnel tests in case of the sections with three different countermeasures mentioned above are conducted and the results compared with the original section show that all the three different countermeasures can be used to control VIV to varying degrees.

Blood Flow Changes by Anastomotic Method in Fontan Operation (폰탄 수술에서 문합방법에 따른 혈류 변화)

  • Kim, S.H.;Park, Y.H.;Cho, B.K.;Kim, J.H.;Hong, Y.S.;Kim, Y.H.;Kim, S.S.
    • Proceedings of the KOSOMBE Conference
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    • v.1996 no.05
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    • pp.203-206
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    • 1996
  • To understand the local fluid dynamics for different desists of Fontan operation, five models were made out of Pyrex glass to facilitate in-vitro study. Model I, II and III have same position of the center of the anastomosis of the IVC( inferior vena cava) with that of the SVC(superior vena cava), but Model IV and V have 10 mm offset between them. Also the anastomotic junction angles are different(Model I and $IV:90^{\circ}$, Model II and $V:70^{\circ}$, Model $III:45^{\circ}$). These models were then connected to a flow loop for flow visualization study. In Model I any dominant vortex was not seen in the central region of the juntion, but a large unstable vortex was created in the Model II and III. In Model IV and V a significant stagnation region was created in the middle of the offset region. It also showed that the flow direction from the IVC and SVC to the LPA(left pulmonary artery) and RPA(right pulmonary artery) highly depends on the offset of the junction rather than the anastomotic junction angle.

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Design of Multi-Phase Shift Controller for Valveless PZT Pump (밸브리스 압전펌프 연동구동 제어기 설계)

  • 조정대;박경민;노종호;함영복;유진산
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2004.10a
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    • pp.1282-1285
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    • 2004
  • The high voltage driving system with multi-phase shifter including piezoelectric actuators comprised a driving power unit for outputting the driving power by converting input alternate current into direct current, a frequency shifting unit for supplying the direct current power and shifting or generating a frequency, a high-voltage amplification unit for amplifying the input signal outputted from the driving power unit and the frequency shifting unit into a high-voltage signal, and a phase shifting unit for shifting the phase difference of the amplified signal applied to the high-voltage amplification unit and driving plural piezoelectric actuators sequentially. The results that the operating voltage was stable, the voltage loss ratio was low and the response velocity was fast could be obtained. An experiment on performance of the high voltage driving system with multi-phase shifter designed and manufactured as above described was conducted by using a piezoelectric pump having 3 sheets of round unimorph piezoelectric actuators laminated respectively in a rectangular case. It sucks any fluid by causing the first piezoelectric actuator to shift from the inlet porter side, the phase delay of 60$^{\circ}$ causes the second piezoelectric actuator to begin to shift, and the phase delay of 120$^{\circ}$ causes the third piezoelectric actuator to begin to shift. As a result of measuring each change in the outlet flow rate of the piezoelectric pump, it was shown that the frequency-flow rate characteristic, the voltage-flow characteristic, and the load pressure-flow rate characteristic were improved.

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A Study of the Influence of Roughness on fracture Shear Behaviour and Permeability (거칠기가 절리의 전단거동 및 투수성에 미치는 영향에 관한 연구)

    • Tunnel and Underground Space
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    • v.12 no.4
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    • pp.312-320
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    • 2002
  • It is well-known that when single rock fractures undergo shear displacement, they are influenced by the boundary conditions and fracture roughness. In this case, aperture geometry will change by means of dilation due to the shear displacement. As fractures become the flow paths, fluid flow through rock fractures is affected by the void geometry. In this study, therefore, the influence of roughness on shear behavior of fractures has been investigated, and the resulting hydraulic behavior has been analyzed. In order for this study, a statistical method has been used to generate rough fractures, and they have been adopted into new conceptual models fur fracture shearing and flow calculations. The main contributions of this study are as follows: firstly, fracture shear behavior becomes less brittle with decreasing fracture roughness and increasing normal stress. Then, the characteristics of aperture distribution becomes those of roughness of fractures indicating its hydraulic significance. Finally, it is observed that with decreasing fracture roughness the breakdown of channel flow occurs more slowly.

Modeling of Flow-Accelerated Corrosion using Machine Learning: Comparison between Random Forest and Non-linear Regression (기계학습을 이용한 유동가속부식 모델링: 랜덤 포레스트와 비선형 회귀분석과의 비교)

  • Lee, Gyeong-Geun;Lee, Eun Hee;Kim, Sung-Woo;Kim, Kyung-Mo;Kim, Dong-Jin
    • Corrosion Science and Technology
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    • v.18 no.2
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    • pp.61-71
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    • 2019
  • Flow-Accelerated Corrosion (FAC) is a phenomenon in which a protective coating on a metal surface is dissolved by a flow of fluid in a metal pipe, leading to continuous wall-thinning. Recently, many countries have developed computer codes to manage FAC in power plants, and the FAC prediction model in these computer codes plays an important role in predictive performance. Herein, the FAC prediction model was developed by applying a machine learning method and the conventional nonlinear regression method. The random forest, a widely used machine learning technique in predictive modeling led to easy calculation of FAC tendency for five input variables: flow rate, temperature, pH, Cr content, and dissolved oxygen concentration. However, the model showed significant errors in some input conditions, and it was difficult to obtain proper regression results without using additional data points. In contrast, nonlinear regression analysis predicted robust estimation even with relatively insufficient data by assuming an empirical equation and the model showed better predictive power when the interaction between DO and pH was considered. The comparative analysis of this study is believed to provide important insights for developing a more sophisticated FAC prediction model.

Packaging Design of EPS Cooling Box by Theoretical Heat Flow and Random Vibration Analysis (이론적 열유동 및 랜덤 진동 해석을 적용한 EPS 보냉용기의 포장설계)

  • Kim, Su-Hyun;Park Sang-Hoon;Lee, Min-A;Jung, Hyun-Mo
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.27 no.3
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    • pp.175-180
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    • 2021
  • Although it has recently been regulated for use as an eco-friendly policy in Korea, the use of EPS (Expanded Polystyrene) cooling boxes, which are used as cold chain delivery insulation boxes for fresh agricultural and livestock products, is also increasing rapidly as e-commerce logistics such as delivery have increased rapidly due to COVID-19. Studies were conducted to optimize the EPS cooling container through internal air heat flow of CFD (Computational Fluid Dynamics) analysis and FEM (Finite Element Method) random vibration analysis using domestic PSD (Power Spectral Density) profile of the EPS cooling box to which the refrigerant is applied in this study. In the analysis of the internal air heat flow by the refrigerant in the EPS cooling box, the application of vertical protrusions inside was excellent in volume heat flow and internal air temperature distribution. In addition, as a result of random vibration analysis, the internal vertical protrusion gives the rigid effect of the cooling box, so that displacement and stress generation due to vibration during transport are smaller than that of a general cooling container without protrusion. By utilizing the resonance point (frequency) of the EPS cooling box derived by the Model analysis of ANSYS Software, it can be applied to the insulation and cushion packaging design of the EPS product line, which is widely used as insulation and cushion materials.

A Numerical Analysis of the Behavior of Liquid Film Around a Rotating Cylinder (회전하는 실린더 주변 액막의 거동에 대한 수치해석적 연구)

  • Lee, Sang-Hyuk;Lee, Jung-Hee;Hur, Nahm-Keon;Seo, Young-Jin;Kim, In-Cheol;Lee, Sung-Jin
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.35 no.5
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    • pp.481-486
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    • 2011
  • It is important to predict the behavior of a liquid film around a rotating cylinder in the film coating process of the steel industry. When the cylinder rotates, the behavior of the liquid film on the rotating cylinder surface is influenced by the cylinder diameter, the rotation speed, the gravitational force, and the fluid properties. These parameters determine the liquid film thickness and the rise of the film on the cylinder surface. In the present study, the two-phase interfacial flow of the liquid film on the rotating cylinder were numerically investigated by using a VOF method. For various rotation speeds, cylinder diameters and fluid viscosities, the behavior of liquid film on the rotating cylinder were predicted. Thicker film around the rotating cylinder was observed with an increase in the rotation speed, cylinder diameter, and fluid viscosity. The present results for the film thickness agreed well with available experimental and analytical results.

Design and Analysis of Piezoelectric Micro-Pump Using Traveling-Wave (진행파를 이용한 압전 마이크로 펌프의 설계와 해석)

  • Na, Yeong Min;Lee, Hyun Seok;Park, Jong Kyu
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.38 no.5
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    • pp.567-573
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    • 2014
  • Since the development of microelectromechanical systems (MEMS) technology for the medical field, various micro-fluid transfer systems have been studied. This paper proposes a micro-piezoelectric pump that imitates a stomach's peristalsis by using two separate piezoelectric elements, in contrast to existing micro-pumps. This piezoelectric pump is operated by using the valve-less traveling wave of peristalsis movement. If the piezoelectric plates at the two separated plates are actuated at the input voltage, a traveling wave occurs between the two plates. Then, the fluid migrates by the pressure difference generated by the traveling wave. Finite element analysis was performed to understand the mechanics of the combined system with piezoelectric elements, elastic structures, and fluids. The effects of design variables such as the chamber height and number of ceramics on the flow rate of the fluid were examined.

Erosion Corrosion Characteristics of Al5052-O and Al6061-T6 Aluminum Alloys with Flow Rate of Seawater (해수 유속 변화에 따른 Al5052-O와 Al6061-T6 알루미늄 합금의 침식부식 특성)

  • Kim, Young-Bok;Kim, Seong-Jong
    • Corrosion Science and Technology
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    • v.18 no.6
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    • pp.292-299
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    • 2019
  • The hull material of a high-speed ship may cause erosion damage from fluid impact. When physical erosion and electrochemical corrosion combine, erosion corrosion damage occurs. The aluminum ship is vulnerable to erosion corrosion because it can be operated at high speed. Thus, in this study, Al5052-O and Al6061-T6 aluminum alloys for the marine environment were selected as experimental materials. The erosion corrosion resistance of Al5052-O and Al6061-T6 aluminum alloys in seawater was investigated by an erosion test and potentiodynamic polarization test at the various flow rate (0 m/s, 5 m/s, 10 m/s, 15 m/s, 20 m/s). Erosion corrosion characteristics were evaluated by surface analysis, 3D analysis, SEM analysis, and the Tafel extrapolation method. The results of surface damage analysis after the erosion test showed that Al6061-T6 presented better erosion resistance than Al5052-O. The results of the potentiodynamic polarization test at the various flow rate, corrosion current density by Tafel extrapolation presented lower values of Al6061-T6 than Al5052-O. Al5052-O showed more surface damage than Al6061-T6 at all flow rates. Consequently, Al6061-T6 presented better erosion corrosion resistance than Al5052-O. The results of this study are valuable data for selecting hull material for an aluminum alloy vessel.