• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.177-177
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• 2017

Phalaenopsis orchid is one of the most important flowers in flower industry. We conducted an experiment to find out the response of different concentration of chlorocholine chloride (CCC) for the in vitro regeneration of protocorm like bodies (PLBs) of Phalaenopsis 'Fmk02010'. We used five different concentrations of CCC and these were 0 (control), 0.01, 0.1, 1 and $10mgL^{-1}$ in modified MS medium and cultured for 42 days. We added two major salts ammonium nitrate ($412.5mgL^{-1}$) and potassium nitrate ($950.0mgL^{-1}$) to the MS medium for the modification. Maximum numbers of PLBs were found from media with $0.01mgL^{-1}$ of CCC (15.667) and maximum fresh weight (0.211 g) as well. The 100% PLB formation rate was also found from $0.01mgL^{-1}$ of CCC. We found 58.83% variation in number of PLBs ($R^2=0.5883$) and 47.44% variation in fresh weight ($R^2=0.4744$) to the different CCC concentrations. Our study suggested that increase in the CCC concentration negatively affect the PLBs organogenesis of Phalaenopsis. We can suggest that the addition of very low concentration of CCC in plant culture medium can increase the number, formation rate and fresh weight of PLBs of Phalaenopsis.

• KIEAE Journal
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• v.12 no.6
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• pp.3-10
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• 2012

Small park in the community is a public asset that is commonly used by the residents daily and performs an important role to activate the community. The purpose of this study is to draw a small park plan through community participation. In concrete, this study tries to find out the demand of the residents about their small park plan, observe the changes in their demand in the process of debate and analyze the effect of the residents' participation. Small group workshop method was adopted in the study and three groups were set up and surveys were made in two stages. Two dimensional pictogram cards and three dimensional digital park formation tools were used. The results of workshop study indicated as follows: Firstly, residents wanted to have community farming garden, green house and flower garden to grow plants, natural walls and safe resting facilities. Secondly, individual and diverse opinions presented at first could be converted to common opinions through mutual exchanges of opinions in the process of debates. Thirdly, digital park formation program functioned well as an effective visual tool to draw the attention and quick responses of the residents and to reach an agreement. It is expected that the results of this study show the effectiveness of residents' participation tools in the process of drawing agreements in the community society that requires the residents' demands and agreement and this study will be usefully utilized in planning community spaces with residents' participation hereafter.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.202-209
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• 2015

In this study, we investigated the cumulative effect of low temperature on bud dormancy release and bud break characteristics in 'Campbell Early' grapevine (Vitis labruscana B.) cuttings grown in water culture. Additionally, we observed the development of buds while exposed to low temperatures in an attempt to improve our understanding of dormancy and bud break. The shoots were collected 120 days after full bloom (DAFB; leaf abscission period), and the accumulated chill unit (CU) value was calculated by reducing the temperature to $7.2^{\circ}C$ at 125 DAFB. The rate of bud break was 100% in shoots collected at 150 DAFB, The period until the first bud break was two times longer than in the shoots collected 165 DAFB, and bud break speed was significantly reduced. These results indicate that buds are released from endodormancy after 165 DAFB, because at this point the bud break was complete (bud break rate 100%) and it occurred in a very short time period. During this period, when the low-temperature accumulated value was 321h and 442CU according to the CH and Utah models, respectively. Furthermore, the survival rate of main buds decreased rapidly after 165 DAFB, and survival rate of accessory buds was maintained at more than 90% without seasonal differences. The rate of flower bud formation of main buds was much higher than in accessory buds (1:0.23) before the release from endodormancy at 150 DAFB. The final ratio of accessory buds to main buds was high, 1:1.54, at 255 DAFB. Correlation analysis of each investigated factor revealed that bud survival rate and bud formation rate were related only for the main buds, and there was a close relationship between the survival rate of main bud and time. In addition, the survival rate of main buds was positively correlated to the rate of flower bud formation.

• Journal of Plant Biotechnology
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• v.46 no.2
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• pp.127-135
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• 2019

The purpose of this study was to investigate suitable parts for callus induction and optimal concentrations of growth regulators, contained in the medium affecting shoot and rooting for in vitro mass production of Haworthia truncata. Leaves and flower bud showed 100% callus formation rate at NAA $1{\sim}2mgL^{-1}$ treatment, and NAA $1mgL^{-1}$ + TDZ $2mgL^{-1}$ treatment. The flower stalk showed 75% callus formation rate, at NAA $2mgL^{-1}$ + TDZ $2mgL^{-1}$ treatment in H. truncata. While the rate of callus formation was high in leaves and flower bud, leaves were the most efficient in obtaining most culture parts. Shoot induction rate from callus was highest, at NAA $0.1mgL^{-1}$ treatment in H. truncata. Additionally, the number of shoots formation was 66.3 shoots high, in NAA $1mgL^{-1}$ + BA $0.1mgL^{-1}$ treatment in H. truncata. In the case of acclimatization of regenerated plant, growth characteristics did not show significant difference (95%) shading with respect to the different ratio of substrate mixture, and it was determined that would be appropriate, considering plant height and appearance preference of H. truncata. It was established that optimization of culture condition, was responsible for mass propagation in vitro cultures of H. truncata.

• Korean Journal of Heritage: History & Science
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• v.43 no.1
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• pp.236-259
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• 2010

The examination of sediment distribution in Seongryu Cave shows existence of rocks contrasting with Joseon Supergroup contrary to existing knowledge. Contrasting especially with the Taeback Group, Daegi Formation, Hwajeol Formation, and Dongjeom Formation has been observed. Unlike Taeback area where Dumugol Formation and Makgol Formation are observed on top of Dongjeom Formation, the rocks of this area are not clear in its separation between the two, so that it was named Geunnam Formation. Seongryu Cave has been developed in this Ordovician Geunnam Formation of the Joseon Supergroup. The cave, mostly horizontal, runs in the NE-SW direction, and contains three lakes. The main passage and branches are about 330 m and 540 m, respectively, making the total length of the cave about 870 m (show cave area = 270 m). Through underwater examination, about 85 m-long underwater passage was newly discovered. Various speleothem such as soda straw, stalactite, stalagmite, column, flowstone, rimston, cave shield, cave coral, curtain, bacon sheet, cave pearl, cave flower, helictite and calcite raft can be found in the cave. There are sections with constant flow of cavern water, but the majority of cavern water in the cave come from the ceiling. The most important discovery in this study is the presence of various speleothem in the submerged part of cave passages. Traces of corrosion and/or erosion can be observed in the speleothem in the submerge passage.

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.156-168
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• 2012

Chrysanthemum is a cut flower species that normally lasts for 1 to 2 weeks, in some cases 3-4 weeks. This has been attributed to low ethylene production during senescence. Reduction in cut flower quality has been attributed to the formation of air embolisms that partially or completely blocks the water transport from the vase solution to the rest of the cut flower stem, increasing hydraulic resistance which may cause severe water stress, yellowing, wilting of leaf, and chlorophyll degradation. Standard type chrysanthemum can be harvested when buds were still tightly closed and then fully opened with the simple bud-opening solution. Standard type chrysanthemum can also be harvested when the minimum size of the inflorescence is about 5-6 cm bud which opened into the first flower full-sized flower. While spray varieties can be harvested when 2-4 most mature flowers have opened (40% opening). Cut flowers are sorted by stem length, weight, condition, and so on. Standard chrysanthemum is 80 cm length for standard type and 70cm for spray type. Pre-treatment with a STS, plant regulator such as GA, BA, 1-MCP, chrysal, germicide, and sucrose, significantly improved the vase life and quality of cut flowers. It is well established that vase solutions containing sugar can improve the vase life of cut chrysanthemum. Chrysanthemum is normally packed in standard horizontal fiberboard boxes. Chrysanthemum should normally be stored at $5{\sim}7^{\circ}C$. Precooling resulted in reduction in respiration, decomposition, and transpiration activities as well as decoloration retardation. There was significant difference between "wet" storage in 3 weeks and "dry" storage in 2 weeks. In separate pulsing solution trials, various germicides were tested, as well as PGRs to maintain the green color of leaves and turgidity. Prolonging vase life was attained with the application of optimal solution such as HQS, $AgNO_3$, GA, BA and sucrose. This also retarded senescence in leaves of cut flower stems. Fresh cut chrysanthemum can be transported using a refrigerated van with $5{\sim}7^{\circ}C$. Increasing consumption and usage of cut chrysanthemum of various cultivars would require efficient transport system, and effective information exchange among producer, wholesaler, and consumer.

• FLOWER RESEARCH JOURNAL
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• v.19 no.1
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• pp.8-14
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• 2011

This study was conducted to investigate the effect of age of transplants, propagated by cutting, of two cut rose (Rosa hybrida Hort.) cultivars on their subsequent growth and yield in an effort to develop an efficient cutting propagation method for domestic rose cultivars. Two cultivars used in this study were a standard type 'Pink Aurora' and a spray type 'Yellow King'. Cuttings were prepared as single node cuttings each with a five-leaflet leaf and were stuck in rockwool cubes ($5cm{\times}5cm{\times}5cm$, UR, Korea) at two different dates. Cuttings rooted for either 30 (stuck on Jan. 20, 2009) or 48 days (stuck on Jan. 2, 2009) were transplanted into a rockwool slabs ($10cm{\times}15cm{\times}100cm$, UR, Korea) on the same date, 18 Feb. 2009. Plant growth and cut flower quality were investigated for two successive harvests during the period of Jan. to July in 2009. In both cultivars, 48 days old plants showed some growth of the shoot and root before transplanting. However, in the case of 30 days old plants before transplanting no noticeable growth of the shoot and root was obserable in 'Pink Aurora', while only shoot growth, but not root growth to the bottom of the rooting medium, was observed in 'Yellow King'. This suggested cultivar-specific responses that in this experiment a spray type 'Yellow King' showed greater growth rate during the rooting stage than a standard type 'Pink Aurora'. In the measurement of growth and cut flower yield after transplanting, the 48 days old standard type 'Pink Aurora' produced greater number of cut flowers per plant than 30 days old plants, whereas their mean stem fresh weight was recorded smaller than that of the 30 days old plants. For 'Yellow King', 30 days old plants showed greater stem length, flower width, number of five-leaflet leaves per stem, stem fresh weight, and number of cut flowers per plant than 48 days old plants. Therefore, growth and yield were significantly affected by cultivar and age of the rooted cutting, and additional research is needed on the effect of age of rooted cuttings in more cultivars.

• Research in Plant Disease
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• v.9 no.3
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• pp.145-148
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• 2003

In April of 2002, the gray mold disease occurred severely on kalanchoe (Kalanch oe blossfeldiana) grown in a nursery in Daesan-myon, Changwon City, Korea. The lesions were started with water-soaked spot and the leaves discolored from the tip and became gray or dark and gradully expanded to whole leaf. The conidia and mycelia of pathogen appearedd on flower, flower stalk and stem. Conidia were gray, 1-celled, mostly ellipsoid or ovoid in shape and were 8~17 ${\times}$5~12${\mu}m$ in size. Conidiophores were 13~34${\mu}m$ in size. The sclerotia were formed abundantly on potato-dextrose agar. The optimum temperature for sclerotial formation was $20^{\circ}C$. Pathogenicity of the causal organism was proved on Kalanchoe blossfeldiana according to Koch,s postulate. The causal organism was identified as Botrytis cinerea based on mycological characteristics. This is the first report on gray mold of Kalanchoe(Kalanchoe blossfeldiana) caused by Botrytis cinerea in Korea.

• Korean Journal of Medicinal Crop Science
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• v.10 no.3
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• pp.217-221
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• 2002

Callus induction and embryogenesis were studied in three different genotypes of Acanthopanax senticosus, to develop a protocol for somatic embryogenesis and acclimatization. Young leaf, stem, node, petiole, peduncle, flower and root explants were collected from 3-year old trees of A. senticosus accessions (Korea, Russia and Japan). Callus was obtained from all cultured explants but showed the higher rate of callus formation in flower cultured. For the three A. senticosus accessions, callus was well formd on MS media containing 2mg/ l of 2,4-D and 2mg/ l of TDZ, 4mg/ l of 2,4-D and 1mg/ l of TDZ than other treatments. For three A. senticosus accessions, when callus transferred to MS medium with 2,4-D, embryogenic cell formed. For A. senticosus accessions Korea, embryogenic cells were obtained on callus induced from petiole, stem, node and root explants, and induction rate was lower than 3%. 200mg of embryogenic callus was transferred to MS free liquid medium and somatic embryos of heart stage were obtained after 45days of culture. When somatic embryo of germination stage were transferred to solid medium, most of the embryos were regenerated into plantlets on 1/4 MS medium. Normal plants with both shoots and roots were transferred to greenhouse soil and were successfully acclimatized.

• Research in Plant Disease
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• v.21 no.4
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• pp.334-336
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• 2015

Sclerotium rot on Pulsatilla koreana was observed in the exhibition field of Jinju Agriculture Technology Center in July 2013. The infected plants showed water-soaked, blighted and rotted symptoms. White mycelial mats spread over lesions, and then numerous sclerotia were formed on flower stalk and flower stem near the soil line. The sclerotia were globoid in shape, 1-3 mm in size and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation on PDA was $30^{\circ}C$ and the hyphal width was $4-8{\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. For molecular identification, the complete ITS rDNA sequence of the causal fungus was sequenced and analyzed. On the basis of mycological characteristics, ITS rDNA sequence analysis, and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report of sclerotium rot on Pulsatilla koreana caused by S. rolfsii in Korea.