• Biomedical Science Letters
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• v.21 no.1
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• pp.40-49
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• 2015

Mesenchymal stem cells (MSCs) have the ability to self-renew and differentiate into multi-lineage cells, thus highlighting the feasibility of using umbilical cord blood-derived MSCs (UCB-MSCs) for cell-therapy and tissueengineering. However, the low numbers of UCB-MSC derived from clinical samples requires that an ex vivo expansion step be implemented. As most stem cells reside in low oxygen tension environments (i.e., hypoxia), we cultured the UCBMSCs under 3% $O_2$ or 21% $O_2$ and the following parameters were examined: proliferation, senescence, differentiation and stem cell specific gene expression. UCB-MSCs cultured under hypoxic conditions expanded to significantly higher levels and showed less senescence compared to UCB-MSCs cultured under normoxic conditions. In regards to differentiation potential, UCB-MSCs cultured under hypoxic and normoxic conditions both underwent similar levels of osteogenesis as determined by ALP and von Kossa assay. Furthermore, UCB-MSCs cultured under hypoxic conditions exhibited higher expression of OCT4, NANOG and SOX2 genes. Moreover, cells expanded under hypoxia maintained a stem cell immnunophenotype as determined by flow cytometry. These results demonstrate that the expansion of human UCB-MSCs under a low oxygen tension microenvironment significantly improved cell proliferation and differentiation. These results demonstrate that hypoxic culture can be rapidly and easily implemented into the clinical-scale expansion process in order to maximize UCB-MSCs yield for application in clinical settings and at the same time reduce culture time while maintaining cell product quality.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.1891-1898
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• 2016

Imperata cylindrica, a tall tufted grass which has multiple pharmacological applications is one of the key ingredients in various traditional medicinal formula used in India. Previous reports have shown that I. cylindrica plant extract inhibited cell proliferation and induced apoptosis in various cancer cell lines. To our knowledge, no studies have been published on the effect of I. cylindrica leaf extract on human oral cancers. The present study was undertaken in order to evaluate the anticancer properties of the leaf extract of I. cylindrica using an oral squamous cell carcinoma cell line SCC-9 as an in vitro model system. A methanol extract from dried leaves of I. cylindrica (ICL) was prepared by standard procedures. Effects of the ICL extract on the morphology of SCC-9 cells was visualized by microscopy. Cytotoxicity was determined by MTT assay. Effects of the ICL extract on colony forming ability of SCC-9 cells was evaluated using clonogenic assay. Cell cycle analysis was performed by flow cytometry and induction of apoptosis was determined by DNA fragmentation assay. The ICL extract treatment caused cytotoxicity and induced cell death in vitro in SCC-9 cells in a dose-dependent manner. This treatment also significantly reduced the clonogenic potential and inhibited cell proliferation by arresting the cell cycle in the G2/M phase. Furthermore, DNA fragmentation assays showed that the observed cell death was caused by apoptosis. This is the first report showing the anticancer activity of the methanol extracts from the leaves of I. cylindrica in human oral cancer cell line. Our data indicates that ICL extract could be considered as one of the lead compounds for the formulation of anticancer therapeutic agents to treat/manage human oral cancers. The natural abundance of I. cylindrica and its wide geographic distribution could render it one of the primary resource materials for preparation of anticancer therapeutic agents.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.2409-2414
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• 2012

Objective: Traditional Chinese herbal medicines have a very long history. Rosa roxburghii Tratt and Fagopyrum cymosum are two examples of plants which are reputed to have benefits in improving immune responses, enhancing digestive ability and demonstrating anti-aging effects. Some evidence indicates that herbal medicine soups containing extracts from the two in combination have efficacy in treating malignant tumors. However, the underlying mechanisms are far from well understood. The present study was therefore undertaken to evaluate anticancer effects and explore molecular mechanisms in vitro. Methods: Proliferation and apoptosis were assessed with three carcinoma cell lines (human esophageal squamous carcinoma CaEs-17, human gastric carcinoma SGC-7901 and pulmonary carcinoma A549) by MTT assay and flow cytometry, respectively, after exposure to extract from Rosa roxburghii Tratt (CL) and extract from Fagopyrum cymosum (FR). $IC_{30}$ of CL and FR were obtained by MTT assay. Tumor cells were divided into four groups : control with no exposure to CL or FR; CL with $IC_{30}$ CL; FR with $IC_{30}$ FR; CL+FR group with 1/2 ($IC_{30}$ CL + $IC_{30}$ FR). RT-PCR and Western blot analysis were used to detect the expression of Ki-67, Bax and Bcl-2 at mRNA and protein levels. Results: Compared with the CL or FR groups, the combination of CL+FR showed significant inhibition of cell growth and increase in apoptosis; the mRNA and protein expression levels of Ki-67 and Bcl-2 in CL+FR group were all greatly decreased, while the expression of Bax was markedly increased. Conclusions: These results indicate that the synergistic antitumor effects of combination of CL and FR are related to inhibition of proliferation and induction of apoptosis.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.611-616
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• 2014

Objectives: Dendritic cell (DC)-based tumor immunotherapy needs an immunogenic tumor associated antigen (TAA) and an effective approach for its presentation to lymphocytes. In this study we explored whether transduction of DCs with lentiviruses (LVs) expressing the human interleukin-12 gene could stimulate antigen-specific cytotoxic T cells (CTLs) against human lung cancer cells in vitro. Methods: Peripheral blood monocyte-derived DCs were transduced with a lentiviral vector encoding human IL-12 gene (LV-12). The anticipated target of the human IL-12 gene was detected by RT-PCR. The concentration of IL-12 in the culture supernatant of DCs was measured by ELISA.Transduction efficiencies and CD83 phenotypes of DCs were assessed by flow cytometry. DCs were pulsed with tumor antigen of lung cancer cells (DC+Ag) and transduced with LV-12 (DC-LV-12+Ag). Stimulation of T lymphocyte proliferation by DCs and activation of cytotoxic T-lymphocytes (CTL) stimulated by LV-12 transduced DCs pulsed with tumor antigen against A549 lung cancer cells were assessed with methyl thiazolyltetrazolium (MTT). Results: A recombinant lentivirus expressing the IL-12 gene was successfully constructed. DC transduced with LV-12 produced higher levels of IL-12 and expressed higher levels of CD83 than non-transduced. The DC modified by interleukin -12 gene and pulsed with tumor antigen demonstrated good stimulation of lymphocyte proliferation, induction of antigen-specific cytotoxic T lymphocytes and antitumor effects. Conclusions: Dendritic cells transduced with a lentivirus-mediated interleukin-12 gene have an enhanced ability to kill lung cancer cells through promoting T lymphocyte proliferation and cytotoxicity.

• Journal of Advanced Navigation Technology
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• v.16 no.5
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• pp.744-751
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• 2012

Air Traffic Management(ATM) is to control air traffic flow quickly and safely. For efficient ATM, the ability which calculate optimal route using filight plan and filght information is required. Especially for the time-sensitive air cargo, it is essential. In this paper, therefore, we present a dynamic route search algorithm which calculate optimal route dynamically. The conventional method using Dijkstra algorithm has a problem that recalculate the entire route when some airways are closed. However, the proposed algorithm recalculates only affected waypoints, so it finds optimal route quickly and accurately. Comparisons with the conventional method and the proposed algorithm show that the proposed algorithm provides better.

• Journal of Korean Neurosurgical Society
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• v.54 no.4
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• pp.280-288
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• 2013

Objective : Perfusion computed tomography (PCT) has the ability to measure quantitative value and produce maps of mean transit time (MTT), cerebral blood flow (CBF), and cerebral blood volume (CBV). We assessed cerebral hemodynamics by using these parameters and acetazolamide (ACZ) challenge for pre- and post-procedural evaluation in patients with unilateral cerebrovascular stenotic disease. Methods : Thirty patients underwent pre-procedural PCT with ACZ challenge, and 24 patients (80%) was conducted follow up PCT after angioplasty with same protocol. The mean MTT, CBF, and CBV were measured and compared in both middle cerebral arterial (MCA) territories before and after ACZ challenge. Hemispheric ratio and percent change after ACZ challenge were calculated before and after angioplasty. Results : The mean stenosis rate was 76.6%. Significant increases in MTT (32.6%, p=0.000) and significant decreases in CBF (-14.2%, p=0.000) were found in stenotic side MCA territories. After ACZ challenge, there were significant changes in MTT (37.4%, p=0.000), CBF (-13.1%, p=0.000), and CBV (-10.5%, p=0.001) in pre-procedural perfusion study. However, no significant increases were found in MTT, or decreases in CBF and CBV in post-procedural study. There were no significant changes after ACZ challenge also. In addition, the degrees of these changes (before and after ACZ challenge) were highly correlated with the stenotic degrees in pre-procedural perfusion study. Conclusion : PCT with ACZ challenge appears to be a useful tool to assess the cerebral perfusion status especially in patients with unilateral symptomatic stenotic disease.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37 no.6B
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• pp.433-442
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• 2012

We have proposed a solution to multicast services and an advanced quality of service (QoS) mechanism on a packet transport system (PTS) based on PBB-TE. The point-to-multipoint (PtMP) connection in the PBB-TE system have been realized by grouping point-to-point (PtP) PTL trunks and mapping a BSI onto the PtP PTL trunks using a multicast backbone destination address. To provide end-to-end QoS of the PtMP services, the hierarchical QoS scheme for backbone service instances and connection-oriented paths has been implemented in the PTS. For providing different capabilities for service selection and priority selection, the PTS offers to customers three basic types of the port-based, C-tagged, and S-tagged service interface defined by the IEEE 802.1ah. To offer to customers different capabilities of the layer 3 applications and services, moreover, an IP-flow service interface have been added. In order to evaluate traffic performance for PtMP services in the PTS, the PtMP throughputs for the link capacity of 1 Gbps at the four service interfaces were measured in the leaves of the ingress edge node, the transit node, and the egress edge node. The throughputs were about 96 % because the B-MAC overhead of 22 bytes occupies 4% of the 512-byte packet. The QoS performance is ability to guarantee an application or a user a required bandwidth, and could be evaluated by the accuracy of policing or shaping. The accuracy of the policing scheme and the accuracy of the shaping scheme were 99% and 99.3% respectively.

• International Journal of Fluid Machinery and Systems
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• v.2 no.4
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• pp.286-294
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• 2009

Hydroelectric power plants are known for their ability to cover variations of the consumption in electrical power networks. In order to follow this changing demand, hydraulic machines are subject to off-design operation. In that case, the swirling flow leaving the runner of a Francis turbine may act under given conditions as an excitation source for the whole hydraulic system. In high load operating conditions, vortex rope behaves as an internal energy source which leads to the self excitation of the system. The aim of this paper is to identify the influence of the full load excitation source location with respect to the eigenmodes shapes on the system stability. For this, a new eigenanalysis tool, based on eigenvalues and eigenvectors computation of the nonlinear set of differential equations in SIMSEN, has been developed. First the modal analysis method and linearization of the set of the nonlinear differential equations are fully described. Then, nonlinear hydro-acoustic models of hydraulic components based on electrical equivalent schemes are presented and linearized. Finally, a hydro-acoustic SIMSEN model of a simple hydraulic power plant, is used to apply the modal analysis and to show the influence of the turbine location on system stability. Through this case study, it brings out that modeling of the pipe viscoelastic damping is decisive to find out stability limits and unstable eigenfrequencies.

• The Korean Journal of Physiology and Pharmacology
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• v.22 no.5
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• pp.481-491
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• 2018

Allergic asthma is one of the most enduring diseases of the airway. The T-helper cells and regulatory T-cells are critically involved in inflammatory responses, mucus hypersecretion, airway remodelling and in airway hyper-responsiveness. Cigarette smoke (CS) has been found to aggravate inflammatory responses in asthma. Though currently employed drugs are effective, associated side effects demand identification and development of novel drugs with negligible or no adverse effects. Rutin, plant-derived flavonoid has been found to possess antioxidant and anti-inflammatory effects. We investigated the ability of rutin to modulate T-cells and inhibit inflammation in experimentally-induced asthma in cigarette smoke exposed mice. Separate groups of neonatal mice were exposed to CS for 10 days from post-natal days 2 to 11. After 2 weeks, the mice were sensitized and challenged with ovalbumin (OVA). Treatment group were given rutin (37.5 or 75 mg/kg body weight) during OVA sensitization and challenge. Rutin treatment was found to significantly inhibit cellular infiltration in the airways and Th2 and Th17 cytokine levels as well. Flow cytometry revealed effectively raised $CD4^+CD25^+Fox3^+$ Treg cells and supressed Th17 cell population on rutin treatment. Airway hyper-responsiveness observed following CS and OVA challenge were inhibited by rutin. $NF-{\kappa}B$ and iNOS, chief regulators of inflammatory responses robustly activated by CS and OVA were down-regulated by rutin. Rutin also inhibited the expression of matrix metalloproteinase 9, thereby aiding in prevention of airway remodelling in asthma thereby revealing to be a potent candidate in asthma therapy.

• Journal of Korean Society on Water Environment
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• v.36 no.5
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• pp.445-452
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• 2020

Tributary is a part of life space for people and a very important place that accommodates rest recreation and other daily activities. absolutely insufficient basic data about water quality and flow rate are available for basin management. Efficient water and basin management systems, which are also supported by local residents can be established by securing such basic data of major tributaries in the Nakdong river system. In this study, the fluctuation characteristics of upstream and downstream water pollution levels were compared using the measurement results of the water environment measurement network and the tributary monitoring project for the gyeseong-stream and Hwapo-stream in the Nakdong-miryang watershed. In 2017, when water pollution is the highest, it was confirmed that the annual average rainfall was the lowest. Although the upstream and downstream water quality tendencies of the Gyeseong-stream are similar, the water quality concentrations of the Gyeseong-stream are relatively different. But although the Hwapo stream has various causes of pollution, there was not much difference in the level of pollution between the upper and lower streams. In addition, both rivers need the ability to purify rivers by securing sufficient water for river maintenance, and if the correlation between water quality items can be inferred through continuous monitoring of tributaries where the aspect of water quality change is unclear, water quality management Determined to be efficient operation.