• Title/Summary/Keyword: flow domain

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Extract from Eucheuma cottonii Induces Apoptotic Cell Death on Human Osteosarcoma Saos-2 Cells via Caspase Cascade Apoptosis Pathway (Eucheuma cottonii 추출물에 의한 인체 골육종암 Saos-2 세포의 자가사멸 유도)

  • Kang, Chang-Won;Kang, Min-Jae;Kim, Kyong Rok;Kim, Nan-Hee;Seo, Yong Bae;Kang, Keon-Hee;Kim, Sang-Ho;Kim, Gun-Do
    • Journal of Life Science
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    • v.26 no.2
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    • pp.147-154
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    • 2016
  • Osteosarcoma (OS) is the most common and malignant bone tumors. Although many types of resection surgery and experimental agents were developed, median survival and clinical prognosis are poorly investigated. Recently, several researches have reported that Eucheuma cottonii has potent as protective effects of coal dust-induced lung damage via inhibition of malondialdehyde (MDA) and oxidative stress in bronchoalveolar lavage fluids (BALF). However, anti-cancer effects and specific molecular mechanism of extract from Eucheuma cottonii (EE) has not been clearly studied yet. This study evaluated that anti-cancer potential of EE in human osteosarcoma Saos-2 cells. EE indicated cytotoxicity on Saos-2 cells in a dose-dependent manner. Morphological degradation and nucleic condensation were also observed under the EE treatment. However, it did not significantly affect on non-cancerous kidney HEK-293 cells under the same concentration which is shown cytotoxicity on Saos-2 cells. The phosphorylation of Fas-Associated Death Domain (FADD) and expression of cleaved caspase-8, -7 and -3 were upregulated in a dose-dependent manner. In immunofluorescence staining, expression level of Fas and cleaved PARP were upregulated by EE treatment. Furthermore, treatment of EE induces upregulation of sub G1 phase by flow cytometry analysis. The results demonstrated that EE has a therapeutic potential against osteosarcoma via FADD mediated caspase cascade apoptosis signal pathway.

A Study about the Direction and Responsibility of the National Intelligence Agency to the Cyber Security Issues (사이버 안보에 대한 국가정보기구의 책무와 방향성에 대한 고찰)

  • Han, Hee-Won
    • Korean Security Journal
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    • no.39
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    • pp.319-353
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    • 2014
  • Cyber-based technologies are now ubiquitous around the glob and are emerging as an "instrument of power" in societies, and are becoming more available to a country's opponents, who may use it to attack, degrade, and disrupt communications and the flow of information. The globe-spanning range of cyberspace and no national borders will challenge legal systems and complicate a nation's ability to deter threats and respond to contingencies. Through cyberspace, competitive powers will target industry, academia, government, as well as the military in the air, land, maritime, and space domains of our nations. Enemies in cyberspace will include both states and non-states and will range from the unsophisticated amateur to highly trained professional hackers. In much the same way that airpower transformed the battlefield of World War II, cyberspace has fractured the physical barriers that shield a nation from attacks on its commerce and communication. Cyberthreats to the infrastructure and other assets are a growing concern to policymakers. In 2013 Cyberwarfare was, for the first time, considered a larger threat than Al Qaeda or terrorism, by many U.S. intelligence officials. The new United States military strategy makes explicit that a cyberattack is casus belli just as a traditional act of war. The Economist describes cyberspace as "the fifth domain of warfare and writes that China, Russia, Israel and North Korea. Iran are boasting of having the world's second-largest cyber-army. Entities posing a significant threat to the cybersecurity of critical infrastructure assets include cyberterrorists, cyberspies, cyberthieves, cyberwarriors, and cyberhacktivists. These malefactors may access cyber-based technologies in order to deny service, steal or manipulate data, or use a device to launch an attack against itself or another piece of equipment. However because the Internet offers near-total anonymity, it is difficult to discern the identity, the motives, and the location of an intruder. The scope and enormity of the threats are not just focused to private industry but also to the country's heavily networked critical infrastructure. There are many ongoing efforts in government and industry that focus on making computers, the Internet, and related technologies more secure. As the national intelligence institution's effort, cyber counter-intelligence is measures to identify, penetrate, or neutralize foreign operations that use cyber means as the primary tradecraft methodology, as well as foreign intelligence service collection efforts that use traditional methods to gauge cyber capabilities and intentions. However one of the hardest issues in cyber counterintelligence is the problem of "Attribution". Unlike conventional warfare, figuring out who is behind an attack can be very difficult, even though the Defense Secretary Leon Panetta has claimed that the United States has the capability to trace attacks back to their sources and hold the attackers "accountable". Considering all these cyber security problems, this paper examines closely cyber security issues through the lessons from that of U.S experience. For that purpose I review the arising cyber security issues considering changing global security environments in the 21st century and their implications to the reshaping the government system. For that purpose this study mainly deals with and emphasis the cyber security issues as one of the growing national security threats. This article also reviews what our intelligence and security Agencies should do among the transforming cyber space. At any rate, despite of all hot debates about the various legality and human rights issues derived from the cyber space and intelligence service activity, the national security should be secured. Therefore, this paper suggests that one of the most important and immediate step is to understanding the legal ideology of national security and national intelligence.

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Development and evaluation of a 2-dimensional land surface flood analysis model using uniform square grid (정형 사각 격자 기반의 2차원 지표면 침수해석 모형 개발 및 평가)

  • Choi, Yun-Seok;Kim, Joo-Hun;Choi, Cheon-Kyu;Kim, Kyung-Tak
    • Journal of Korea Water Resources Association
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    • v.52 no.5
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    • pp.361-372
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    • 2019
  • The purpose of this study is to develop a two-dimensional land surface flood analysis model based on uniform square grid using the governing equations except for the convective acceleration term in the momentum equation. Finite volume method and implicit method were applied to spatial and temporal discretization. In order to reduce the execution time of the model, parallel computation techniques using CPU were applied. To verify the developed model, the model was compared with the analytical solution and the behavior of the model was evaluated through numerical experiments in the virtual domain. In addition, inundation analyzes were performed at different spatial resolutions for the domestic Janghowon area and the Sebou river area in Morocco, and the results were compared with the analysis results using the CAESER-LISFLOOD (CLF) model. In model verification, simulation results were well matched with the analytical solution, and the flow analyses in the virtual domain were also evaluated to be reasonable. The results of inundation simulations in the Janghowon and the Sebou river area by this study and CLF model were similar with each other and for Janghowon area, the simulation result was also similar to the flooding area of flood hazard map. The different parts in the simulation results of this study and the CLF model were compared and evaluated for each case. The results of this study suggest that the model proposed in this study can simulate the flooding well in the floodplain. However, in case of flood analysis using the model presented in this study, the characteristics and limitations of the model by domain composition method, governing equation and numerical method should be fully considered.

Development of Rapid-cycling Brassica rapa Plant Program based on Cognitive Apprenticeship Model and its Application Effects (인지적 도제 모델 기반의 Rapid-cycling Brassica rapa 식물 프로그램의 개발 및 적용 효과)

  • Jae Kwon Kim;Sung-Ha Kim
    • Journal of Science Education
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    • v.47 no.2
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    • pp.192-210
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    • 2023
  • This study was intended to develop the plant molecular biology experimental program using Rapid-cycling Brassica rapa (RcBr) based on the teaching steps and teaching methods of the cognitive apprenticeship model and to determine its application effects. In order to improve a subject's cognitive function and expertise on molecular biology experiments, two themes composed of a total 8 class sessions were selected: 'Identification of DFR gene in purple RcBr and non-purple RcBr' and 'Identification of RcBr's genetic polymorphism site using the DNA profiling method'. Research subjects were 18 pre-service teaching majors in biology education of H University in Chungbuk, Korea. The effectiveness of the developed program was verified by analyzing the enhancement of 'cognitive function' related to the use of molecular biology knowledge and technology, and the enhancement of 'domain-general metacognitive abilities.' The effect of the developed program was also determined by analyzing the task flow diagram provided. The developed program was effective in improving the cognitive functions of the pre-service teachers on the use of knowledge and technology of molecular biology experiments. It was especially effective to improve the higher cognitive function of pre-service teachers who did not have the previous experience. The developed program also showed a significant improvement in the task of metacognitive knowledge and in the planning, checking, and evaluation of metacognitive regulation, which are sub-elements of domain-general metacognitive abilities. It was found that the developed program's self-test activity could help the pre-service teachers to improve their metacognitive regulation. Therefore, this developed program turned out to be helpful for pre-service teachers to develop core competencies needed for molecular biology experimental classes. If the teaching and learning materials of the developed program could be reconstructed and applied to in-service teachers or high school students, it would be expected to improve their metacognitive abilities.

Characterization of Bruton's Tyrosine Kinase Genetic Mutations in One Korean X-linked Agammaglobulinemia Family (반성 열성 범저감마글로불린혈증 1가계 3환자의 Bruton's Tyrosine Kinase 유전자 변이 및 임상 양상)

  • Jo, Eun-Kyeong;Song, Chang-Hwa;Park, Jeong-Kyu;Baek, Young-Jong;Rhu, Hye-Young;Lee, Jae-Ho;Hwang, Tai-Ju;Kook, Hoon
    • Clinical and Experimental Pediatrics
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    • v.45 no.2
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    • pp.183-191
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    • 2002
  • Purpose : X-linked agammaglobulinemia(XLA) is an immunodeficiency caused by abnormalities in Bruton's tyrosine kinase(Btk), and is characterized by a deficiency of peripheral blood B cells. We studied the cytoplasmic expression of Btk protein and analyzed the Btk gene in peripheral blood mononuclear cells from two siblings and one cousin with XLA, as well as additional family members. Methods : Btk protein expression was analyzed by flow cytometry. Isolation of the coding sequence of the Btk gene was performed by amplification using the reverse transcription-polymerase chain reaction(RT-PCR) technique. Sequence alterations were screened by the single-stranded conformation polymorphism(SSCP) method and characterized by standard sequencing protocols. Results : Cytoplasmic expression of Btk protein in monocytes was not detected in three patients with XLA. In addition, Btk protein analysis clearly showed cellular mosaicism in monocytes from four obligate carriers, findings further supported by SSCP. A single base pair mutation(T to C) in Btk-exon three, which encodes the PH domain, was identified in four XLA patients. A diagnostic sequencing analysis was established to detect heterozygotic pattern in 4 carrier females. Furthermore, we found significant clinical heterogeneity in individuals with the same gene mutation. Conclusion : The implicating genetic alteration provided valuable clues to the pathogenesis of XLA in Korea and the flow cytometric analysis was suggested as a useful tool for rapid detection of XLA patients and carriers. The present study has identified a genetic mutation in the Btk coding region and demonstrated heterogeneity in clinical manifestations among patients with the same mutation. A flow cytometric analysis was found to be informative in establishing a deficiency of Btk protein in both patients and carriers and is recommended as a frontline procedure in the molecular diagnosis and work-up of XLA.

Study on Effect of Convection Current Aeration System on Mixing Characteristics and Water Quality of Reservoir (대류식 순환장치의 저수지수체 유동특성 및 수질영향)

  • Lee, Yo-Sang;Lee, Kwang-Man;Koh, Deok-Koo;Yum, Kyung-Taek
    • Korean Journal of Ecology and Environment
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    • v.42 no.1
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    • pp.85-94
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    • 2009
  • This study examines the operational effectiveness of a Convection Current Aeration System (CCAS) in reservoir. CCAS was run from June, 2008 when the thermocline begun forming in the reservoir. This paper reviews the influence of stratification, dissolved oxygen dynamics and temperature in the lake's natural state from June to October 2008. The survey was done on a week basis. Upwelling flow effects a radius of $7{\sim}10m$ at a surface directly and was irrelevant to the strength of thermocline. On the other hand, it was affected the number of working days, and strength of thermocline at vertical profiles of the reservoir. Longer CCAS run, the deeper was the vertical direct flow area. However it didn't break the thermocline during summer season of 2008. The operating efficiency of the CCAS in the reservoir depends on hydraulics and meteological conditions. Computational Fluid Dynamics (CFD) is a very useful tool for evaluating the operating efficiency of fluid dynamics. The geometry for CFD simulation consists of a cylindrical vessel 25 m radius and 40 m height. The CCAS is located in center of domain. The non-uniform tetrahedral meshes had a bulk of the geometry. The meshes ranged from the coarse to the very fine. This is attributed to the cold water flowing into the downcomer and rising, creating a horizontal flow to the top of the CCAS. The result of CFD demonstrate a closer agreement with surveyed data for temperature and flow velocity. Theoretical dispersion volume were calculated at 8m depth, 120 m diameter working for 30 days and 10 m depth, 130 m diameter working for 50 days.

Transport of Zn Ion under various pH Conditions in a Sandy Soil (사질토양에서의 pH조건에 따른 Zn의 이동특성)

  • Park, Min-Soo;Kim, Dong-Ju
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.1
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    • pp.33-42
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    • 2000
  • Adsorption onto the surfaces of solid particles is a well known phenomenon that causes the retardation effect of heavy metals in soils. For adequate remediation of soil and groundwater contamination, it is important to investigate the mobility of heavy metals that largely depends on pH conditions in the soil water since adsorption of heavy metals is pH-dependent. In this study, we investigated the transport of Zn ion under various pH conditions in a sandy soil by conducting batch and column tests. The batch test was performed using the standard procedure of equilibrating fine fractions collected from the soil with eleven different initial $ZnCl_2$ concentrations, and analysis of Zn ion in the equilibrated solutions using ICP-AES. The column test consisted of monitoring the concentrations of soil solutions exiting the soil column with time known as a breakthrough curve (BTC). We injected respectively $ZnCl_2$ and KCl solutions with the concentration of 10 g/L as a tracer in a square pulse type under three different pH conditions (7.7, 5.8, 4.1) and monitored the flux concentration at the exit boundary using an EC meter and ICP-AES. The resident concentration was also monitored at the 10cm-depth by Time Domain Reflectometry (TDR). The results of batch test showed that ion exchange process between Zn and other cations (Ca, Mg) was predominant. The retardation coefficients obtained from adsorption isotherms (Linear, Freundlich, Langmuir) resulted in the various values ranging from 1.2 to 614.1. No retardation effect but ion exchange was found for the BTCs under all pH conditions. This can be explained by the absence of other cations to desorb Zn ion from soil exchange sites under the conditions of ETC experiment imposing blank water as leachate in steady-state flow. As pH decreased, the peak concentration of Zn increased due to the competition of Zn with hydrogen ions ($H^+$) and the concentrations of other cations decreased. The peak concentration of Zn was increased by 12.7 times as pH decreased from 7.7 to 4.1.

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Hydrologic evaluation of SWAT considered forest type using MODIS LAI data: a case of Yongdam Dam watershed (MODIS LAI 자료를 활용하여 임상별로 고려한 SWAT의 수문 평가: 용담댐유역을 대상으로)

  • Han, Daeyoung;Lee, Jiwan;Kim, Wonjin;Baek, Seungchul;Kim, Seongjoon
    • Journal of Korea Water Resources Association
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    • v.54 no.11
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    • pp.875-889
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    • 2021
  • This study compares and analyzes the Soil and Water Assessment Tool (SWAT) and Terra MODIS (Moderate Resolution Imaging Spectroradiometer) as coniferous, deciduous and mixed forest with Yongdam Dam upstream (904.4 km2). The hydrologic evaluation period was set to 10 years from 2010 to 2019, and the applicability of the 8-day MOD15A2 Leaf Area Index (LAI) data, 3 TDR (Time Domain Reflectometry) (GB, JC, CC), and 1 Flux Tower (DU) evaporation volume (YDD) data was simulated. As a result, the R2 of coniferous forest, deciduous forest and mixed forest are 0.95, 0.89, 0.90, soil moisture and evaportranspiration stations R2 were analyzed at 0.50 to 0.55 and 0.51, respectively, with R2 at 0.74, RMSE 2.75 mm/day, NSE 0.70 and PBIAS 14.3% for Yongdam inflow. Based on the calibrated and validated watersheds, the annual average evaportranspiration was calculated as coniferous 469.7 mm, deciduous 501. mm and 511.5 mm mixed forest, total runoff were estimated at coniferous 909.8 mm, deciduous 860.6 mm and 864.2 mm mixed forest. In the case of annual average evaportranspiration, it was evaluated that deciduous were high, but in the case of streamflow, it was evaluated that coniferous were high. Unlike other hydrologic with similar patterns throughout the year, the average annual evapotranspiration was about 7% higher than coniferous due to the higher evapotranspiration of deciduous with high leaf area index in summer and fall. In addition, deciduous were 9% and 6% higher for surface runoff and lateral flow, but the groundwater of coniferous was 77% higher. Therefore, it was confirmed that the total runoff was in order of coniferous, mixed forest, and deciduous.

Analysis and modeling of thermal resistance of multi fin/finger FinFETs (멀티 핀/핑거 FinFET 트랜지스터의 열 저항 해석과 모델링)

  • Jang, MoonYong;Kim, SoYoung
    • Journal of the Institute of Electronics and Information Engineers
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    • v.53 no.8
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    • pp.39-48
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    • 2016
  • In this paper, we propose thermal resistance compact model of FinFET structure that has hexagon shaped source/drain. The heating effect and thermal properties were increased by reduced size of the device, and thermal resistance is an important factor to analyze the effect and the properties. The heat source and each contact that is moved heat out were set up in transistor, and domain is divided by the heat source and the four parts of contacts : source, drain, gate, substrate. Each contact thermal resistance model is subdivided as a easily interpretable structure by analyzing the temperature and heat flow of the TCAD simulation results. The domains are modeled based on an integration or conformal mapping method through the structure parameters according to its structure. First modeled by analyzing the thermal resistance to a single fin, and applying the change in the parameter of the channel increases to improve the accuracy of the thermal resistance model of the multi-fin/ finger. The proposed thermal resistance model was compared to the thermal resistance by analyzing results of the 3D Technology CAD simulations, and the proposed total thermal resistance model has an error of 3 % less in single and multi-finl. The proposed thermal resistance model can predict the thermal resistance due to the increase of the fin / finger, and the circuit characteristics can be improved by calculating the self-heating effect and thermal characterization.

Method for Evaluating Radionuclide Transport in Biosphere by Calculating Elapsed Transport Time (이동 경과 시간 계산을 이용한 생물권에서의 방사성 핵종 이동 평가 방법)

  • Ko, Nak-Youl;Ji, Sung-Hoon
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.18 no.2_spc
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    • pp.305-315
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    • 2020
  • For geological disposal of radioactive wastes, a method was proposed to evaluate the radionuclide transport in the biosphere by calculating the elapsed time of nuclide migration. The radionuclides were supposed to be introduced from a natural barrier and reached a large surface water body following a groundwater flow in a shallow subsurface. The biosphere was defined as a shallow subsurface environment that included aquifers on a host rock. Using the proposed method, a calculation algorithm was established, and a computer code that implemented the algorithm was developed. The developed code was verified by comparing the simulation results of the simple cases with the results of the analytical solution and a public program, which has been widely used to evaluate the radiation dose using the radionuclide transport near the surface. A case study was constructed using the previous research for radionuclide transport from the hypothetical geological disposal repository. In the case study, the code calculated the mass discharge rate of radionuclide to a stream in the biosphere. Because the previous research only demonstrated the transport of radionuclides from the hypothetical repository to the host rock, the developed code in the present study could help identify the total transport of radionuclide along the complete pathway.