• The Journal of Engineering Geology
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• v.15 no.3
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• pp.257-268
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• 2005

In situ rock mass is generally heterogeneous and discontinuous, with varying degrees of strength along the planes of weakness. The planes of weakness such as joints, faults, cracks and bedding planes, control the strength and deformation characteristics of the rock mass. Subsequently, the stability of underground opening depends upon the spatial distribution of discontinuities and their mechanical properties in relation with geometrical shape of openins as well as the mechanical properties of intact rock materials. Understanding the behaviour of a discontinuous rock mass remains a key issue for improving excavation design in hiかy stressed environments. Although recent advances in rock mechanics have provided guidelines for the design of underground opening in isotropic rock mass, prediction and control of deformation in discontinuous rock masses are still unclear. In this study, parametric study was performed to investigate the plastic zone size, stress distribution and deformation behavior around underground opening in a discontinuous rock mass using a continuum joint model. The solutions were obtained by an elasto-plastic finite difference analysis, employing the Mohr-Coulomb failure criteria. Non-associated flow rule and perfectly plastic material behavior are also assumed.

• KSBB Journal
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• v.16 no.3
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• pp.286-289
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• 2001

A beterotrophic Pseudomonas putida B2 was used to treat of hydrogen sulfide containing gas. The experimental approach involved operating two indentical bench-scale biofilters with media consisting of a mixture of peatmoss, perlite and granular activated carbon(GAC). One column was seeded with Pseudomonas putida B2 and the other was left unseeded. The biofilter was operated for 16 days under EBRT for 20-40 sec, at a temperature of 25-30$^{\circ}C$ and a hydrogen sulfide concentration of 40-190 ppm. The biofilter inocculated with P.putida B2 exhibited high hydrogen sulfide removal efficiency, average of 95%, at a gydrogen sulfide concentration of 40-190 ppm (flow rate 3.6 L/min). However, at a shock loading of 190 ppm the biofiter showed a removal efficiency of 78.9% and the control only showed a removal efficiency of 31.6%. The critical load of this biofilter was 14.83 g/㎥hr, and the critical load of the control column was 4.93 g/㎥hr. These results suggest that P. putida B2 has the potential to be used as a $H_2S$ removal agent in a biofilter.

• Tuberculosis and Respiratory Diseases
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• v.67 no.6
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• pp.528-535
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• 2009

Background: Dust clouds blown by the wind from the arid deserts of Mongolia and Northeast China are known as Asian dust storms. Ambient particulate matter with a diameter <10 ${\mu}m$ ($PM_{10}$) is associated with the exacerbation of respiratory diseases and increased mortality of heart and lung disease patients. The fibrotic effects of $PM_{10}$ of Asian dust to pulmonary fibroblast cells are unknown. This study examined the production of reactive oxygen species (ROS), TGF-${\beta}$, NF-${\kappa}B$, PDGF-$\alpha$ and Fibronectin in fibroblasts exposed to Asian dust particles. Methods: Air samples were collected using a high volume air sampler (Sibata model HV500F) with an air flow of 500 L/min for at least 6 hours. The MRC-5 cells were exposed to 0, 50 and 100 ${\mu}g/mL$ of $PM_{10}$ for 24 hours. ROS was detected by measuring the level of oxidized DCF using FACS. TGF-$\beta$, NF-${\kappa}B$, PDGF-$\alpha$ and fibronectin were detected by western blotting. Results: There was no increase in the ROS, TGF-$\beta$ and PDGF-$\alpha$ levels in the MRC-5 cells exposed to $PM_{10}$. The NF-${\kappa}B$ level was higher in the MRC-5 cells exposed to 50 and 100 ${\mu}g/mL$ of $PM_{10}$ for 24 hours. The fibronectin level in the MRC-5 cells after 24 hours incubation with 50 ${\mu}g/mL$ $PM_{10}$ was significantly higher than the control group ($PM_{10}$ 50 ${\mu}g/mL$ 113.27${\pm}$8.65 of control, p=0.005). Conclusion: $PM_{10}$ from Asian dust increases the activation of NF-${\kappa}B$ and fibronectin expression in MRC-5 fibroblast cells.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.4
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• pp.562-568
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• 2016

This study investigated the effects of Yuza pectin extract (0, 0.1, 0.15, and 0.2%, w/v) on the physicochemical, microbial, rheological, and sensory properties of yogurt. Pectin extract was extracted from Yuza peel by using citrate after juicing the flesh. The total sugar content of Yuza pectin was 89.07%. The pH of Yuza pectin extract-added yogurt was 4.43. All Yuza pectin extract-added yogurt samples showed shear-thinning flow behaviors (n=0.33~0.44). The values for apparent viscosity ($0.34{\sim}0.47Pa{\cdot}s$), consistency index ($4.48{\sim}10.25Pa{\cdot}s^n$), yield stress (6.56~17.61 Pa), storage modulus (47.96~75.21 Pa), and loss modulus (19.79~26.06 Pa) for 0.1~0.2% (w/v) Yuza pectin extract-added yogurt were considerably higher than those of the control. These result indicated that Yuza pectin extract could enhance steady and dynamic shear rheological properties of yogurt. The sensory scores of Yuza pectin extract-added yogurt were higher than those of the control.

• Horticultural Science & Technology
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• v.16 no.3
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• pp.347-349
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• 1998

Slow sand filtration was modified and applied for the determination of eliminating efficacy of various fungi and for recommending an easy approach to growers. After 1,500 liter filtration, Fusarium oxysporum was eliminated by several substrates such as activated charcoal (92.5% elimination), silica (90.8%), vermiculite (90.5%), sand (82.3%), perlite (50.4%), and hydroball (21.2%). Silica was able to eliminate several fungi by maximal ratio, which was corresponded to Fusarium oxysporum 120 cfu/mL. Collectotrichum lagenarium 98 cfu/mL. Phytophthora capsici 82 cfu/mL, Botrytis cinerea 62 cfu/mL, Pythium spp. 42 cfu/mL, and Sclerotinia ssp. 52 cfu/mL. In this case, the change of EC was minor and pH was maintained to about 7. In deep flow culture of 'Ddooksum Cheokchookmyeon' lettuce and 'Seokwang' tomato, silica-, activated charcoal-, and vermiculite-based filtration system successfully eliminated Fusarium oxysporum and Phytophthora capsici from the nutrient solution. As a result, these plants were not diseased by ten weeks after inoculation. With this system, growers can easily control the root-zone fungi in the recirculating hydroponic system.

• Molecules and Cells
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• v.43 no.10
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• pp.856-869
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• 2020

To elucidate the mechanism of action of HOXA11-AS in modulating the cisplatin resistance of nasopharyngeal carcinoma (NPC) cells. HOXA11-AS and miR-454-3p expression in NPC tissue and cisplatin-resistant NPC cells were measured via quantitative reverse transcriptase polymerase chain reaction. NPC parental cells (C666-1 and HNE1) and cisplatin-resistant cells (C666-1/DDP and HNE1/DDP) were transfected and divided into different groups, after which the MTT method was used to determine the inhibitory concentration 50 (IC₅₀) of cells treated with different concentrations of cisplatin. Additionally, a clone formation assay, flow cytometry and Western blotting were used to detect DDP-induced changes. Thereafter, xenograft mouse models were constructed to verify the in vitro results. Obviously elevated HOXA11-AS and reduced miR-454-3p were found in NPC tissue and cisplatin-resistant NPC cells. Compared to the control cells, cells in the si-HOXA11-AS group showed sharp decreases in cell viability and IC₅₀, and these results were reversed in the miR-454-3p inhibitor group. Furthermore, HOXA11-AS targeted miR-454-3p, which further targeted c-Met. In comparison with cells in the control group, HNE1/DDP and C666-1/DDP cells in the si-HOXA11-AS group demonstrated fewer colonies, with an increase in the apoptotic rate, while the expression levels of c-Met, p-Akt/Akt and p-mTOR/mTOR decreased. Moreover, the si-HOXA11-AS-induced enhancement in sensitivity to cisplatin was abolished by miR-454-3p inhibitor transfection. The in vivo experiment showed that DDP in combination with si-HOXA11-AS treatment could inhibit the growth of xenograft tumors. Silencing HOXA11-AS can inhibit the c-Met/AKT/mTOR pathway by specifically upregulating miR-454-3p, thus promoting cell apoptosis and enhancing the sensitivity of cisplatin-resistant NPC cells to cisplatin.

• Analytical Science and Technology
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• v.17 no.5
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• pp.401-409
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• 2004

The 7-keto-DHEA-acetate is converted to 7-keto-DHEA, a metabolite of DHEA, and similar to its metabolism. We studied the metabolite M3, M4, and M5 of 7-keto-DHEA-acetate. The estimated molecular weight of M3 and M4 was 304 which were supposed to have more 3 hydroxyl and/or ketone groups. We could know that M3 is the 7-OH-DHEA which has the hydroxyl groups on 3 and 7-carbon and a ketone group on 17-carbon. In case of M4, it is the 7-oxo-diol metabolite which has the hydroxyl groups on 3 and 17-carbon and a ketone group on 7-carbon. The M5 was supposed that the molecular weight is 320 and has the three hydroxyl groups on 3, 6, and 16 carbon and the ketone group on 17-carbon. After dosing, 7-OH-DHEA showed the maximum urine flow in human urine after 5 hr and decreased rapidly. But we could find it until 58 hr why is a higher remaining substance.

• Journal of Bio-Environment Control
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• v.8 no.3
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• pp.192-201
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• 1999

This study was aimed to introduce the measurement of $CO_2$ concentration and leaf area index in the phytotron for predicting the effect of CO.E, light and leaf area index on the instantaneous photosynthetic rate of sweet pepper with the existing ASKAM model. Measurements were made in 2 semi-closed phytotron compartments in which three different $CO_2$ concentrations were applied at random. Plants were grown on containers with circulating nutrient solution at 21$^{\circ}C$ and 80-95% relative humidity. The model estimates crop net $CO_2$ uptake for short time intervals during the day based on short-term data of daily radiation, temperature and $CO_2$ concentration. During the photosynthesis measurements, $CO_2$ concentrations in both compartments and in the basement were measured every minute. This was also done for the flow of pure $CO_2$ into the compartment, global radiation, photosynthetic active radiation inside the compartment, temperature and relative humidity. Crop growth models summarize our knowledge on crop behavior and have as such a wide range of applications in analysis, crop management and thus as a farm management tool.

• Journal of Bio-Environment Control
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• v.25 no.2
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• pp.123-132
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• 2016

The importance of energy saving technology for managing greenhouse was recently highlighted. For practical use of energy in greenhouse, it is necessary to simulate energy flow precisely and estimate heating/cooling loads of greenhouse. So the main purpose of this study was to develope and to validate greenhouse energy model and to estimate annual/maximum energy loads using Building Energy Simulation (BES). Field experiments were carried out in a multi-span plastic-film greenhouse in Jeju Island ($33.2^{\circ}N$, $126.3^{\circ}E$) for 2 months. To develop energy model of the greenhouse, a set of sensors was used to measure the greenhouse microclimate such as air temperature, humidity, leaf temperature, solar radiation, carbon dioxide concentration and so on. Moreover, characteristic length of plant leaf, leaf area index and diffuse non-interceptance were utilized to calculate sensible and latent heat exchange of plant. The internal temperature of greenhouse was compared to validate the greenhouse energy model. Developed model provided a good estimation for the internal temperature throughout the experiments period (coefficients of determination > 0.85, index of agreement > 0.92). After the model validation, we used last 10 years weather data to calculate energy loads of greenhouse according to growth stage of greenhouse crop. The tendency of heating/cooling loads change was depends on external weather condition and optimal temperature for growing crops at each stage. In addition, maximum heating/cooling loads of reference greenhouse were estimated to 644,014 and $756,456kJ{\cdot}hr^{-1}$, respectively.

• Journal of Korean Society for Atmospheric Environment
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• v.30 no.6
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• pp.586-599
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• 2014

During the last two decades, indoor air quality and volatile organic compounds (VOCs) have been of concern in Korean society due to their nature of potential health impacts. In order to investigate the pollution levels of VOCss in indoor environments, establishment of a solid test method for monitoring the airborne VOCss is essential. In Korea, a method based on adsorbent sampling and GC analysis coupled with thermal desorption was proclaimed as the Korea Standard Method for Indoor Air Quality Test. This study was carried out to examine some inherent problems of the VOCs measurement method. The VOCs method does not describe in detail preparing the standard samples. The standard samples may be prepared by impregnation of either liquid standard solutions or a mixture of standard gases. In this study, we investigated the optimal temperature condition for transferring the liquid standards onto a standard adsorbent tube. As a result, keeping the impregnation temperature at $250^{\circ}C$ will be recommended in regard of the boiling points of multiple target analytes and the thermal stability of the adsorbent. We also demonstrated some problems associated with handling of a syringe used for transferring the standard solutions onto the adsorbent tubes, and a best way to get rid of the syringe problems was suggested. Finally, a number of field works were conducted to evaluate the performance of adsorbent sampling methods. Comparison of different adsorbent tubes, i.e. tube packed with single sorbent (Tenax) and double sorbents (Tenax with Carbotrap), revealed that 30 to 40% differences between the two groups, implying that sampling efficiency is depending on the volatility and the strength of adsorbents. However, duplicate precisions for VOCs sampling with a same type of adsorbent and at same flow rates appeared to be satisfactory to be all within 20%, which is a quality control guideline. Distributed volume precisions were also found to be within a guideline value, 25%, although the precision was in general inferior to the duplicate precision. The Korea indoor VOCs test method should be more refined and improved in many aspects, particularly procedure and instrumentation for preparing the standard samples and specification of quality control assessment.