• The Journal of Internal Korean Medicine
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• v.26 no.2
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• pp.310-319
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• 2005

Objectives: The aim is to identify any anti-tumor effects of Duchesnea indica(Andr.) Focke on colon cancer cells. Materials & Methods: Colo201 human adenocarcinoma cells were obtained from American Type Culture Collection. The boiled extract of Duchesnea indica(Andr.) Focke was added (10 and 20 microliters) to cultures and observed at 0, 6, and 12 hours, and at 12-hour intervals thereafter. Morphological changes in colon cancer cells were observed through an inverted microscope, Destruction of colon cancer cells was measured through Trypan blue exclusion testing. Suppression of the viability of colon cancer cells were measured via MTT assay. Anti-cancer mechanisms in the cell cycle of colon cancer cells were analysed via flow cytometry. Results: After introduction of Duchesnea indica(Andr.) Focke to cultures several changes were seen. Significant atrophy of the nucleus and cytoplasm of colon cancer cells was observed, indicating cell injury. Destruction of colon cancer cells was observed in direct proportion to dosage and duration. Suppression of viability of colon cancer cells for each test group was greater than that of the control group increasingly over time(36h, 48h, 60h, 72h), which was statistical significant (p<0.05). Cell numbers of the mitosis phase of the colon cancer cell cycle reduced. Conclusions: Statistcally significant anti-tumor effects of Duchesnea indica(Andr.) Focke were observed in this in vitro experiment. Results support a role for Duchesnea indica(Andr.) Focke in treatment of colon cancer. though it will required progressive research to develop a practical treatment.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.2
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• pp.580-585
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• 2004

BJYGC is often clinically used as a treatment of allergic rhinitis. This study was aimed to find out the effect BJYGC would have on the helper T cell, and how it can promote the subsets of helper T cells to regain their balance that they lost due to immunological diseases. Splenocytes were prepared from BALB/c mice was cultured without stimulation in the presence of BJYGC for 48 hr. The viability of CD4 T cells from Balb/c mouse were measured at various concentrations of BJYGC using the MTS assay. It was somewhat increased up to concentration of 400 ㎍/ml, but did not show any significant difference. Proliferation was measured using the MTS assay, CD4 Th cells were stimulated with anti-CD3/28 in the presence of BJYGC for 48 hr. As evidence for rapid T cell activation, CD25 expression by flow cytometry was evaluated at 10, 50, 100 and 200 ㎍/㎖ of BJYGC. Th cell differentiation experiments were performed to examine whether BJYGC can affect the Th polarization process. CD4 T cells were activated in culture under neutral, Th1-polarized or Th2-polarized conditions in the presence of BJYGC at 10, 100 and 200 ㎍/㎖. Cytokine production was measured by ELISA. This experiment proved that BJYGC could inhibit the secretion of both IL-4 and IFN-γ in neutral condition and polarized condition, too. Considering that BJYGC shows an excellent effect on treating allergies, the author can conclude that its pharmacological action may be associated with decreased IL-4 and, it may also regulate IFN-γ depending the host's need. Also, it was discovered that Th1 cell was pathologic in chronic inflammatory tissue specific diseases, such as insulin dependent diabetes mellitus, multiple sclerosis, RA, and uveitis. We are counting on the BJYGC to be able to control the tendency of Th1 cell predominancy in an immune reaction.

• Journal of Korean Society of Transportation
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• v.22 no.2 s.73
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• pp.95-102
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• 2004

The COSMOS is an adaptive traffic control systems that can adjust signal timing parameters in response to various traffic conditions. To evaluate the performance of the COSMOS systems, the field study is only practical option because any evaluation tools are not available. To overcome this limitation, a newly integrated interfacing simulator between a microscopic simulation program and COSMOS was developed. In this paper, a detector module and a signal timing module as well as general feature of the simulator were described. A validation test was performed to verify the accuracy of the data flow within the simulator. It was shown that the accuracy level of information from the simulator was high enough for real application. Several practical comments on further studies were also included to enhance the functional specifications of the simulator.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.36 no.9
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• pp.825-834
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• 2008

Nose chine shape optimization study has been performed to maximize the directional stability at high angle of attack supersonic flow. Various chine shapes are generated using super ellipse equation. By numerically investigating the directional stability characteristics of those shapes, the baseline configuration for the shape optimization has been selected using the three-dimensional Navier-Stokes equations. The configuration is represented by the NURBS curves which can adjust the surface geometry by the control points. The response surfaces are constructed to obtain optimum shape which has high directional stability characteristics and lift-to-drag ratio. From this study, an efficient configuration design and optimization process which utilizes the parameter-based configuration generation techniques and approximation method has been established, then 29% improvement of the directional stability by strong vortexes from chine nose is accomplished.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.36 no.8
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• pp.728-733
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• 2008

Unsteady aerodynamic analysis of an air-pressure-levitated high-speed ground vehicle moving over the nonplanar ground surface are performed using the boundary-element method. The potential flow solution is included in a time-stepping loop and the wake is captured as part of the solution. When the vehicle moving inside the channel, the lift coefficient and the pitching moment coefficient of the vehicle are increased further because the air trapped by the channel increases the ground effect. In other words, the nonplanar ground surface such as the channel decreases further the longitudinal stability of the vehicle. On the other hand, there is little difference between the ground and the channel in the lateral stability of the vehicle because the lift increment due to the nonplanar ground surface such as the channel takes place on both sides of the wing with the same rate of increase.

• Toxicological Research
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• v.17 no.2
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• pp.131-137
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• 2001

Baicalein, a major flavonoid of extract from Scutellaria baicalensis Georgi, has been shown to exhibit antioxidant and anti proliferative effects. In the present study, we investigate the effects of baicalein on viability and induction of apoptosis in human promyelocytic leukemia HL-60 cells. Baicalein was found to induce apoptosis of HL-60 cells in a concentration-dependent and time-dependent manner. When HL-60 cells were exposed to 100 $\mu\textrm{M}$ baicalein for 6h, the viability was decreased remarkably to 27% of control, whereas DNA fragmentation was significantly increased to 64%. Nucleosomal fragmentation of baicalein treated HL-60 cells, a hallmark of apoptosis, was further identified by agarose gel electrophoresis (DNA ladder). Flow cytometric analysis showed that apoptotic cells were increased to 66.6% after treatment with 100 $\mu\textrm{M}$ baicalein for 6 h. Baicalein-induced apoptosis of HL-60 cells was reduced by 1h pretreatment with inhibitor of caspases, z-Asp-$CH_2$-DCB. At 3 and 10 $\mu\textrm{M}$ of z-Asp-$CH_2$-DCB, DNA fragmentation of HL-60 cells induced by baicalein (50 $\mu\textrm{M}$) was 36.8 and 17.1 %, respectively, whereas, that of HL-60 cells treated by baicalein (50 $\mu\textrm{M}$) without pretreatment with inhibitor of caspases was 62.7%. These data suggest that baicalein induces apoptosis in human leukemia HL-60 cells, and that caspase enzymes might be involved in baicalein-induced apoptosis.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.4 no.2
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• pp.62-73
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• 2001

The riparian environments of urban streams in Korea have been disturbed through the channelization for flood control and artificial land use as well as water pollution and flow decrease due to industrialization and urbanization. The flora and vegetation structure were investigated and an implication of stream restoration was discussed for the conservation of biodiversity in the riparian area of the Seunggi stream in Incheon. Naturalized plants and ruderal plants were widely distributed in the riparian area which was disturbed from cultivating, trampling, dumping etc. Submerged and floating hydrophytes were not found in the stream due to channelization and water pollution. Some halophytes were remained in downstream and reservoir after reclamation and embankment. The communities of Humulus japonicus, Panicum dichotomiflorum, Digitaria sanguinalis, Artemisia montana, Amaranthus retroflexus, and Aster pilosus were distributed in the disturbed area of bank slope and floodplain in the stream. As a natural potential vegetation, Phragmites australis in the wet meadow, Typha latifolia, Typha angustifolia, Oenanthe javanica, Persicaria thunbergii, and Penthorum chinense in the marsh, and Salix babylonica and Salix matsudana for. tortuosa in the woodland appeared in the floodplain. The topography in the stream played an important role on the distribution of riparian vegetation in the Seunggi stream. Appropriate methods for conservation and restoration of the riparian ecosystems must be planned on the basis of the actual vegetation in the disturbed urban stream.

• Korean Journal of Food Science and Technology
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• v.24 no.5
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• pp.428-439
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• 1992

Drying process in a fixed bed red pepper dryer was modeled and simulated. Drying efficiency describing the effectiveness of energy usage in red pepper drying was defined as a ratio of energy used for moisture evaporation to total energy consumption, and expressed in combination of measurable temperature variables. The efficiency was compared with real evaporative efficiency and tested in the simulated and experimental drying. An overall drying efficiency was derived, and analyzed for various control variables consisting of drying temperature, air recycle ratio and air flow rate. Optimal operation conditions of drying was then searched by Box's complex method by using it as an objective function. Carotenoids retention was simulated and put as a constraint of product quality in the optimization. The optimization results gave that two staged drying operation could improve the ding efficiency compared with single staged drying. As a technique for further energy saving automatic termination of drying appeared feasible by monitoring an exit air temperature from dryer.

• Journal of Korean Society for Atmospheric Environment
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• v.32 no.5
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• pp.526-535
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• 2016

Recently, fine dust in atmosphere have been considerably issued as a harmful element for human. Nitrogen oxide ($NO_x$) exhausted from diesel engines and power plants has been disclosed as a main source of secondary production of fine dust. In order to prevent exhausting these nitrogenous compounds into atmosphere, a treatment system with selective catalytic reduction (SCR) catalyst with ammonia as a reductant has been used in various industries. Urea solution has been widely studied to supply ammonia into a SCR catalytic reactor, safely. However, the conversion of urea solution to ammonia has several challenges, especially on a slow conversion velocity. In the present study, a fast urea conversion system including a plasma burner was suggested and designed to evaluate the performances of urea conversion and initial operation time. A designed lab-scale facility has a plasma burner, urea nozzle, mixer, and SCR catalyst which is for hydrolysis of isocyane. Flow rate of methane that is a fuel of the plasma burner was varied to control temperatures in the urea conversion facility. From experimental results, it is found that urea can be converted into ammonia using high temperature condition of above $400^{\circ}C$. In the designed test facility, it is found that ammonia can be produced within 1 min from urea injection and the result shows prospect commercialization of proposed technology in the SCR facilities.

• Journal of Microbiology and Biotechnology
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• v.19 no.8
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• pp.803-809
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• 2009

Recent evidence has revealed the occurrence of an apoptotic phenotype in Candida albicans that is inducible with environmental stresses such as acetic acid, hydrogen peroxide, and amphotericin B. In the present study, we found that the Chinese herbal medicine Baicalein (BE), which was one of the skullcapflavones, can induce apoptosis in C. albicans. The apoptotic effects of BE were detected by flow cytometry using Annexin V-FITC and DAPI, and it was confirmed by transmission electron microscopy analysis. After exposure to 4 ${\mu}g$/ml BE for 12 h, about 10% of C. albicans cells were apoptotic. Both the increasing intracellular levels of reactive oxygen species (ROS) and upregulation of some redox-related genes (CAP1, SOD2, TRR1) were observed. Furthermore, we compared the survivals of CAP1 deleted, wild-type, and overexpressed strains and found that Cap1p attenuated BE-initiated cell death, which was coherent with a higher mRNA level of the CAP1 gene. In addition, the mitochondrial membrane potential of C. albicans cells changed significantly (p<0.001) upon BE treatment compared with control. Taken together, our results indicated that BE treatment induced apoptosis in C. albicans cells, and the apoptosis was associated with the breakdown of mitochondrial membrane potential.