• Archives of Pharmacal Research
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• 제16권3호
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• pp.196-204
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• 1993

Flavonol derivatives were tested for their anticlastogenic effect against induction of micronuclei by n-methyl-n'-nitor-n-nitorsoguanidine(MNNG), and against induction of chromosome aberration by bleomycin or MNNG.belomycin. For micronudeus assay, each flavonol derivative (0, 0.001, 0.01, 0.1, 1, 10 and 100 mg/kg) was administered orally twice with 24 h interval, together with intraperitioneally administered MNNG(150 mg/kg). The result showed that msot flavonol derivatives tested were effective in suppresing the frequencies of micronude induced by MNNG. For chromosome aberration assy, each flavonol derivative (0, 0.1, 1, 10m and 100 mg/kg)was administered to mice orally in vivo, and then mice were sacrificed and spleen lymphocyte cultures were made. Bleomycin $(3\;\mu$g/ml) was treated to the mouse spleen hymphocyte cultures at 24 h after con A initiation. There wre nomarked decrease tendencies in chromosome aberration unless all doses of galangin and some doses of several flavonol derivatives tested. In the another experiment, we have evaluated the effect of flavonol derivatives on the enhancement of bleomycin-induced chromsome aberration by MNNG. Most of flavonol derivtives reduced the incidence of chromosome aberration induced by in vitro treatment of bleomycin followed by in vivo treatment of MNNG. Galangin particulary showed a dose-dependent decrease tendency. Other flavonol derivative showed slightly suggest that most of flavonol derivatives may be capable of protecting the inhibition of suggest that most of flavonol derivatives may be capable of protecting the inhibition of DNA-repair by MNNG. Our data indicate clearly that flavonol derivatives can suppress MNNG-induced genotoxicity such as an induction of MNPCEs. Therfore, our results could suggest that flavonol derivtives may be useful as a chemopreventive agent of MNNG.

• KSBB Journal
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• 제8권1호
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• pp.55-61
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• 1993

Ginkgo biloba의 세포배양을 통하여 세포성장과 flavonol glycosides의 생성에 영향을 미치는 여러 조건들을 연구하였다. 다양한 지역으로부터 유도된 세포주들의 세포성장과 flavonol glycosides의 생합성 능력은 서로 차이가 있었다. 이들의 캘러스 및 현탁 세포는 배지조건과 배양조건에 따라 성장 및 f1avonol glycosides 생합성에 많은 영향을 받았었다. 특히 배양조건 중 빛의 영향은 현저하여 명조건에서의 f1avonol glycosides의 생성은 암조건보다 10배 이상 증가함을 알 수 있었다.

• 한국약용작물학회지
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• 제13권6호
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• pp.250-254
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• 2005

Night-break, 일장조절, 월동가온 실험으로부터 얻어진 바위솔에 포함된 flavonol glycoside, $kaempferol-3-O-{\beta}-D-glucoside$(1), $kaempferol-3-O-{\beta}-D-glactoside$ (2), $kaempferol-3-O-{\beta}-D-rhamnoside$ (3), $quercetin-3-O-{\beta}-D-glucoside$ (4) and $quercetin-3-O-{\alpha}-D-rhamnoside$ (5)의 함량이 분석되었으며 자연산 바위솔의 함량과 비교되었다. 월동가온 시험에서 얻은 flavonol glycoside 1-5의 함량은 자연산보다 감소하였으며 일장조절 실험 및 night-break 시험에서 얻은 flavonol glycoside 함량은 조사한 빛의 양이 증가함에 따라 증가하였다. 월동가온은 flavonol glycoside의 생성에 부정적으로 작용하는 반면 조사되는 빛의 양의 증가와 같은 경작조건은 flayonol glycoside의 생성에 유리하다.

• 생약학회지
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• 제24권1호
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• pp.47-53
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• 1993

The seasonal variations of the major six flavonol glycosides(kaempferol 2,6-dirhamnosyl glucoside, quercetin 3-O-rutinoside, kaempferol 3-O-rutinoside, isorhamnetin 3-O-rutinoside, quercetin 3-O-coumaroyl glucorhamnoside and kaempferol 3-O-coumaroyl glucorhamnoside) in Ginkgo biloba leaves were investigated. The contents were determined by HPLC on reversed phase $C_{18}$ column. This result showed that the percentage of six flavonol glycosides decreased during the season from 1.57% in May to 0.39% in November. The content of each flavonol glycoside indicated a similar tendency to decrease. However, the contents of rutinosides of kaempferol, quercetin and isorhamnetin fluctuated markedly than those of coumaroyl glucorhamnosides of kaempferol and quercetin and kaempferol 2,6-dirhamnosyl glucoside.

• 디지털융복합연구
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• 제13권8호
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• pp.503-514
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• 2015

은행잎 유래 플라보놀 성분이 갖는 항아토피 활성에 대해 입증된 바는 드물다. 이 논문에서는 MC/9 비만세포에서의 은행잎 플라보놀의 항아토피 효과를 조사하기 위하여 ELISA와 Real-time PCR, western blot으로 은행잎 플라보놀을 분석하였다. 분석 결과 IL-13, MIP-1a의 생성량은 농도 의존적으로 현저하게 감소되었으며 IL-4, IL-5, IL-13, TNF-a 유전자 발현량은 25, 50, $100{\mu}g/m{\ell}$의 농도에서 효과적으로 억제되었다. western blot 분석 결과 c-jun과 NFAT-1단백질 발현이 감소되었음을 확인하였다. 이러한 결과들은 은행잎 유래 플라보놀 성분이 NFAT-1, c-jun 전사인자의 전사를 억제함으로써 MC/9 비만세포에서의 Th2 싸이토카인의 생성을 감소시키는 효과를 갖는 것을 나타낸다. 따라서 은행잎 유래 플라보놀 성분이 아토피 피부염 치료제로서 활용가능성이 있음을 보고하고자 한다.

• 생약학회지
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• 제43권3호
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• pp.206-212
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• 2012

Ten flavonol glycosides were isolated from the EtOAc fraction of the MeOH extract of Metaplexis japonica Makino. Structures of the flavonoids were elucidated on the basis of spectroscopic data and comparison with literature values. The flavonoids were found to be mostly common flavonol 3-glycosides. It is of interest that the sacchaide parts of the isolates were pairs of arabinosides, glucosides, galactosides, rutinosides and robinobiosides of kaempferol and quercetin. All of these compounds were isolated for the first time from this plant.

• Archives of Pharmacal Research
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• 제22권4호
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• pp.423-427
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• 1999

A study was carried out to evaluate flavonol glycosides in leaves of Symplocarpus renifolius (Araceae). From the water fraction of the MeOH extract, three new flavonol glycosides were isolated along with three known compounds, Kaempferol-3-O-$\beta$-glucopyranosyl-($1{\rightarrow}2$)-$\beta$-D-glucopyranosyl-7-O-$\beta$-D-glucopyranoside, quercetin-3-O-$\beta$-D-glucopyranosy-1-($1{\rightarrow}2$)-$\beta$-D-glucopyranoside, and caffeic acid. The structures of the new flavonol glycosides were elucidated by chemical and spectral analyses a quercetin-3-O-$\beta$-D-glucopyranosyl-($1{\rightarrow}2$)-$\beta$-D-glucopyranosyl-7-O-$\beta$-D-glucopyranoside, isorhamnetin-3-O-$\beta$-D-glucopyranosyl-(1 2)-$\beta$-D-glucopyranosyl-7-O-$\beta$-D-glucopyranosdie, and quercetin-3-O$\beta$-D-glucopyranosyl-($1{\rightarrow}2$)-$\beta$-D-glycopyranosyl-7-O-($6^{IIII}$-trans-caffeoyl)-$\beta$-D-glucopyranoside.

• 한국작물학회지
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• 제59권3호
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• pp.385-389
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• 2014

국내외 75점의 흰색 및 자색 양파 유전자원으로부터 총 5종의 flavonol 배당체(Q34'diG, Q3G, Q4'G, I4'G, Q)를 분리 동정하였다. 양파 내 존재하는 flavonol 배당체는 aglycone인 quercetin 및 isorhamnetin에 하나 또는 두 개의 glucose가 결합된 형태로 검출되었다. 총 flavonol 함량 범위는 흰색 양파가 0.18-6.47 mg/g, 자색 양파가 2.39-6.47 mg/g 이었으며, 자색 양파가 평균 4.41 mg/g 로 흰색 양파(3.23 mg/g)보다 약 1.4배 높았다. 양파의 주요 성분으로는 Q34'diG 및 Q4'G 총함량 비율이 흰색 및 자색 양파에서 모두 약 85% 이상을 보였다.

• Natural Product Sciences
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• 제6권4호
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• pp.199-200
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• 2000

A flavonol diglucoside was isolated from the leaves of Brassica juncea L. The structure of the compound was elucidated as isorhamnetin $3,7-di-O-{\beta}-D-glucopyranoside$ (1) on the basis of chemical and spectral evidence.

• Natural Product Sciences
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• 제11권1호
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• pp.10-12
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• 2005

Flavonol galactosides were isolated from the EtOAc fraction of Artemisia apiacea by repeated column chromatography. Their structures were elucidated as $isorhamnetin-3-O-{\beta}-D-galactoside$ (1) and $quercetin-3-O-{\beta}-D-galactoside$ (2) by chemical and spectroscopic analysis. This is the first report on the isolation of compound 2 from this plant.