• 한국환경농학회지
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• 제36권1호
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• pp.7-16
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• 2017

BACKGROUND: Increased value added by rapeseed (Brassica napus) by-product and the development of a usable rapeseed functional tea. METHODS AND RESULTS: To develop a usable rapeseed functional tea, the total flavonoid content in the varieties Youngsan, Tammi, Tamra, Naehan, Hanra, Mokpo No. 68, and Mokpo No. 111 was investigated. Effect of three treatments, i.e., drying, leaching, and roasting, on flavonoid contents or flower was tested using multiple processing methods per treatment. Total flavonoid content decreased under the various drying methods, confirming that flavonoid content is heat-dependent. This finding was more pronounced for freezing and oven-drying (15.3 and 13.8 mg/g DW, respectively), with a 10% difference in the total flavonoid content between the two methods. Under leaching conditions, the flavonoid content decreased with increasing treatment time. Notably, roasting methods did not result in loss of flavonoid content. The total flavonoid content in the rapeseed varieties decreased in the following order: Youngsan, Tammi, Tamra, Naehan, Hanra, Mokpo No. 68, and Mokpo No. 111. CONCLUSION: The flavonoid content in rapeseed flower was higher in Youngsan than in the other varieties, under processing conditions such as freeze-drying, leaching at $90^{\circ}C$ for 5 min, and roasting.

• Natural Product Sciences
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• 제22권1호
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• pp.25-29
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• 2016

High-performance liquid chromatography was performed in order to analyze the changes in the flavonoid content (rutin, hyperin, afzelin, quercetin, and kaempferol) of Acanthopanax divaricatus and A. koreanum, in response to different cultivation methods (pinching height, planting time, and top dressing). The total flavonoid content of A. divaricatus and A. koreanum ranged from 0.201 to 0.690 mg/g with different pinching heights, 0.143 to 1.001 mg/g for different planting times, and 0.156 to 1.074 mg/g depending on the rate of fertilizer application. In both A. divaricatus and A. koreanum, the total flavonoid content in the upper section of the plant was greater than that in the lower section. These results demonstrate which cultivation methods maximize the flavonoid content of A. divaricatus and A. koreanum, and thus help to optimize flavonoid yields to improve production for nutraceutical, pharmaceutical, and cosmeceutical applications.

• 한국미생물·생명공학회지
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• 제33권4호
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• pp.288-294
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• 2005

다양한 생리활성을 갖는 flavonoid 화합물이 감귤에 비활성 형태의 배당체 화합물로 다량으로 존재하고 있으므로 비활성 형태의 flavonoid 물질을 활성 형태의 flavonoid 무배당체 물질로 변환 시켜 활성 형태의 flavonoid 화합물을 증가하고자 하였다. 무배당체 화합물로 전환하기 위하여 시판되고 있는 당 분해효소 중 amylase가 주성분인 AMG 300L, pectinase가 주성분인 Pectinex 100L 및 cellulase, $\beta$-glucosidase, xylanase가 주성분인 Viscozyme 등을 단독 또는 혼합 처리하여 생리활성이 우수한 무배당체 화합물의 함 량을 증가할 수 있었다. 감귤에 함유되어 있는 flavonoid 화합물의 함량은 naringenin이 $100\∼200\;ng/g$(감귤건조중량), hesperetin이 1$100\∼220\;ng/g$(감귤건조중량) 정도이었으나 당분해효소를 처리한 감귤에 함유되어 있는 활성형 flavonoid 화합물인 naringenin이 $1539\∼6674\;ng/g$(감귤건조중량), hesperetin이 $1974\∼8906\;ng/g$(감귤건조중량)으로서 본 실험 결과를 통해 감귤 추출물의 flavonoid 화합물의 함량을 약 $10\∼80$배 정도로 증가시킬 수 있었다. 당 분해 효소에 의한 무배당체로의 최적 전환조건으로는 당 분해 효소의 $5\%$ 첨가, pH $5\∼7$이었으며 효소 반응 시간으로는 $24\∼48$시간이 가장 우수하였다. 따라서 본 연구에 의해 제조된 감귤 조성물에는 생리활성이 우수한 활성형의 무배당체 flavonoid인 naringenin과 hesperetin을 다량으로 함유하고 있어 기능성 식품, 건강 보조식품, 가공 식품의 식품 원료나 사료 첨가제 의 용도로 유용하게 사용할 수 있다.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.353-358
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• 2023

Barley (Hordeum vulgare) belongs to the Poaceae family. This study compared the polyphenol and flavonoid levels of unfermented and fermented barley sprouts using spectrophotometric assays. The findings indicated that fermentation greatly boosted the flavonoid content but caused only a slight increase in the polyphenol content. However, this does not imply that fermentation has no effects whatsoever on the polyphenol content of barley sprouts. This was due to the fact that some flavonoids cannot be detected by the wavelength used to calculate the overall polyphenol concentration. Both samples were subjected to high-performance liquid chromatography analysis and detected the flavonoids lutonarin, saponarin, isoorientin, isovitexin, and tricin-all of which have bioactive properties-most notably known for their antioxidant activity. These results augment the ongoing phytochemical profiling research and can possibly valorize the already thriving barley industry.

• 한국응용과학기술학회지
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• 제33권3호
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• pp.521-528
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• 2016

The antioxidant activity of 70% methanol, 70% ethanol and chloroform-methanol (CM, 2:1, v/v) extracts from turmeric (Curcuma longa L.) was investigated in this study. The antioxidant potential of various extracts of turmeric was evaluated by using different antioxidant tests, namely DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, ABTS [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity, ferric reducing antioxidant power and ${\beta}$-carotene bleaching assays as well as total phenol content and total flavonoid content. All extracts exhibited abundant total phenol content, total flavonoid content and strong antioxidant activity. Particularly, higher activity was exhibited by CM extract with $46.32{\pm}0.35mgCAE/g$ and $15.58{\pm}0.39 mgQE/g$ of total phenol content and total flavonoid content. Generally, antioxidant activity of various extracts from turmeric increased in the following order: 70% methanol extract, 70% ethanol extract and CM extract, respectively. Results suggested that turmeric could be widely used as a source of antioxidant and a kind of functional material in food.

• Journal of Applied Biological Chemistry
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• 제65권2호
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• pp.89-92
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• 2022

In this study, the effects of soil conditions on antioxidant activities of the aerial and underground parts of rosemary were assessed to determine the most effective soil conditions for cultivation. The antioxidant activity was the highest (51.58±2.93 ㎍/mL) when cultivated in the mixture of gardening soil and vermiculite using DPPH assay. The antioxidant activity of underground parts the highest (127.48±12.38 ㎍/mL) when cultivated in the mixture of soil, vermiculite, and perlite. ABTS assay showed that the antioxidant activity of aerial parts was 230.34±57.93 ㎍·mL^-1 when cultivated in the mixture of gardening soil and vermiculite and that of underground parts was 320.98±16.04 ㎍·mL^-1 when cultivated in the mixture of gardening soil, vermiculite, and perlite. The total phenolic content of aerial parts was the highest (155.25±2.96 mg GAE/g) when cultivated in the mixture of gardening soil. The total flavonoid content of aerial parts was the highest (67.32±5.27 mg QE/g) when cultivated in the mixture of gardening soil. Therefore, the mixture of gardening soil, vermiculite, and perlite is superior to gardening soil alone for cultivation of rosemary to increase its antioxidant activity as well as total phenolic and flavonoid content.

• 한국식품조리과학회지
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• 제21권2호
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• pp.190-194
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• 2005

This study was conducted to investigate the changes in contents of total flavonoid and total polyphenol of three leafy vegetables, spinach(Spinacia oleracea L.), chard(Beta vulgaris L.), whorled mallow(Malva verticillata L.) by various blanching times. Total flavonoid and total polyphenol contents of spinach were 21.01 and 25.58 mg/g, those of chard were 26.02 and 35.14 mg/g, and those of whorled mallow were 16.80 and 19.94 mg/g, respectively. Total flavonoid and total polyphenol contents of chard were the highest among the leafy vegetables. Total flavonoid and total polyphenol contents of leafy vegetables were decreased according to blanching time. Especially, total flavonoid content of chard showed significant loss of $15-30\%$ by blanching time. Total polyphenol content of whorled mallow showed loss of $37-39\%$, but the decrease was not significant.

• Food Science and Biotechnology
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• 제17권6호
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• pp.1387-1390
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• 2008

This study evaluated the effects of heat treatment on antioxidant activity of Rehmannia radix Libosch (RRL). RRL was heated at various temperatures ($110-150^{\circ}C$) for various times (1-5 hr), and the total polyphenol, flavonoid content, and antioxidant activity were investigated. With increased heating temperature and exposure time, total content of polyphenol, flavonoid, as well as antioxidant activity increased. The highest total polyphenol and flavonoid contents were 21.65 and 3.56 mg/g, respectively, these values were occurred after heating for 3 hr at $150^{\circ}C$ (RRL was 5.09 and 0.83 mg/g, respectively). The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was highest value of 83.46% after heating for 3hr at $150^{\circ}C$. The 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation radical scavenging activity was highest value of 20.43mg ascorbic acid (AA) eq/g after heating for 2 hr at $150^{\circ}C$. There were highly significant differences in the total polyphenol, flavonoid content, and antioxidant activity among heating temperatures and times (p<0.001), with heating temperature having the greater effect.

• 한국식생활문화학회지
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• 제27권1호
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• pp.75-81
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• 2012

This study was conducted to investigate the physiological activity of acai berry (Euterpe oleracea Mart.) extracts from three different solvents (water, methanol, and ethanol). We measured total polyphenol and total flavonoid content, DPPH radical scavenging activity, nitrite scavenging activity, metal chelating effect, and reducing power. The extraction yield from water, methanol, and ethanol was 17.10, 9.50, and 37.51%, respectively. The highest total polyphenol content (10.54 mg/100 g) and total flavonoid contents (1.88 mg/100 g) was observed in water extract. DPPH radical scavenging activity was the highest in both water extract (72.03%) and methanol extract (74.79%) at levels of 5 mg/mL, which was similar to that of BHT (78.90%). Water extract yielded the highest metal chelating effect (92.54%) and reducing power (1.09) at levels of 5 mg/mL. Taken together, these findings suggest that extracts of acai berry can be used as functional food materials with antioxidative and nitrite scavenging activities.

• 한국식품영양학회지
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• 제33권1호
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• pp.64-73
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• 2020

The purpose of this study was to investigate the antioxidant activity and β-glucan content of extracts extracted by varying the temperature at 30, 55 and 80℃ after hot air drying or freeze drying of Pleurotus ostreatus and Pleurotus eryngii. For the analysis antioxidant activity of each mushroom, β-glucan, total phenol, flavonoid contents, and DPPH·ABTS⁺·Nitrite assay were measured. Also, the β-glucan content, total flavonoid content and ABTS⁺ scavenging activity increased with freeze drying rather than hot air drying, and increased with increasing extraction temperature in both mushrooms. However, the total phenol and nitrite scavenging activity increased with hot air drying rather than freeze drying, and decreased with increasing temperature in both mushrooms. DPPH scavenging activity was not significant in both mushrooms, but decreased with increasing extraction temperature. Pearson's correlations between total flavonoid content and antioxidant activities were r=0.719~0.753 (p<0.01). As a result, the β-glucan content, total flavonoid content, and ABTS radical cation scavenging activity were highest during freeze drying and extraction at 80℃. And the highest total phenol content, DPPH radical scavenging activity and nitrite scavenging activity were obtained during hot air drying and extraction at 30℃.