• 제목/요약/키워드: fish mortality

검색결과 396건 처리시간 0.026초

An outbreak of Motile Aeromonas Septicemia in cultured Nile tilapia, Oreochromis niloticus with reference to hematological, biochemical and histopathological alterations

  • Korni, Fatma M.M.;EL-Nahass, EL-Shaymaa;Ahmed, Walaa M.S.
    • 한국어병학회지
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    • 제30권1호
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    • pp.11-24
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    • 2017
  • The current investigation dealing with the causative agent of mass mortalities in cultured Oreochromis niloticus. The diseased fish showed external hemorrhage, unilateral and bilateral eye opacity, ended by blindness and fish death. The postmortem lesions revealed congested friable kidney and spleen, and liver has yellow nodules. Obtained isolates were identified as Aeromonas hydrophila (the causative agent of Motile Aeromonas Septicemia) and found to be highly pathogenic as they contained hemolysin virulence gene causing mortality reached to 100 and 70% in intraperitoneal and intramuscular infection. The prevalence of MAS was 80% among the surveyed O. niloticus. Blood and serum were collected from naturally diseased, intraperitoneal and intramuscular injected O. niloticus for hematological and biochemical examination. Similarly, gills, musculature, kidney, liver and spleen were collected for histopathological evaluation, and micropathomorphological analysis of spleen was done. Macrocytic hypochromic anemia was recorded in the intraperitoneal infection. Serum protein, albumin and globulin were decrease only in naturally diseased fish. Leucocytosis with heterophilia and lymphocytosis were observed in naturally diseased and intraperitoneal infected fish. There were severe degenerative changes and hemorrhagic necrosis in the examined tissues which were more obvious in intraperitoneal than intramuscular infection. Activation and proliferation of melanocytes macrophages centers with severe hemosiderosis were recorded in spleen of naturally diseased and experimentally infected fish.

Population dynamics and stock status of spotted sardinella (Amblygaster sirm, Walbaum, 1792) in the Natuna Sea, Indonesia

  • Moh Fauzi;Tirtadanu;Andina Ramadhani Putri Pane;Siti Mardlijah;Tegoeh Noegroho;Achmad Zamroni;Thomas Hidayat;Heri Widiyastuti;Budi Nugraha;Hufiadi;Prawira Atmaja Tampubolon
    • Fisheries and Aquatic Sciences
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    • 제27권4호
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    • pp.256-264
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    • 2024
  • Small pelagic fish populations have long been exploited by various fishing gears in the Natuna Sea, and since the 1970s, purse seine exploitation has expanded to offshore fishing grounds. Various small pelagic fish species are exploited from these waters including spotted sardinella (Amblygaster sirm). This research was conducted for 22 months, from March 2019 to December 2020. The fish sample was taken from the purse seine fleet's catch in the fisheries management area (FMA) 711 Natuna Sea and Karimata Strait, which was brought to Pemangkat fishing port in the West Kalimantan Province, Indonesia. This paper aims to determine the exploitation status of spotted sardinella in the Natuna Sea as a recommendation material for management policies. The methods used in this analysis are estimated age, cohort, population parameters, and spawning potential ratio of spotted sardinella. The results show three primary cohorts of spotted sardinella in the Natuna Sea, with ages ranging from 9 months to 3.1 years and a maximum attainable age (longevity) of 4.3 years. The asymptotic value (L) is 21.79 cmFL in length, with a growth rate (K) of 0.65 per year. The natural mortality rate (M) was 1.19, the total mortality rate (Z) was 2.19, and the fishing mortality rate (F) was 0.99. The exploitation rate (E) was 0.45 and the stock status was still in a sustainable condition. Therefore, the sardinella fisheries can be developed using a precautionary approach and focus on the ecosystem sustainability of FMA 711.

물고기의 성장도를 예측하는 FGRS(Fish Growth Regression System) (FGRS(Fish Growth Regression System), Which predicts the growth of fish)

  • 원성권;심용보;손수락;정이나
    • 한국정보전자통신기술학회논문지
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    • 제16권5호
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    • pp.347-353
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    • 2023
  • 양식장에서 물고기의 성장을 측정하는 작업은 아직도 사람의 손이 많이 가는 방식을 사용한다. 이 방식은 많은 노동력이 필요하고, 물고기가 스트레스를 받아 폐사율에 악영향을 준다. 이러한 문제를 해결하기 위해 물고기의 성장도를 자동화하기 위한 시스템 FGRS(Fish Growth Regression System)를 제안한다. FGRS는 두 개의 모듈로 구성된다. 첫째는 Yolo v8 기반의 물고기를 디텍팅하는 모듈이고, 둘째는 물고기 영상 데이터를 CNN 기반의 신경망 모델을 이용하여 물고기의 성장도를 예측하는 모듈로 구성된다. 시뮬레이션 결과 학습전에는 예측 오차가 평균 134.2일로 나왔지만 학습 이후 평균 오차가 39.8일 까지 감소했다. 본 논문에서 제안한 시스템을 이용해 생육일을 예측하여 물고기의 성장예측을 활용해 양식장에서의 자동화에 기여할 수 있고, 많은 노동력 감소와 비용 절감 효과를 가져 올 수 있을 것이라 기대한다.

나일 틸라피아에 대한 질병예방제 및 면역보조제로서의 PS-K 효과 분석 (Effect of PS-K on nile tilapia, Oreochromis niloticus, as an adjuvant and prophylactic agent)

  • 박경현;하재이;허민도;허성회;정현도
    • 한국어병학회지
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    • 제10권1호
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    • pp.45-52
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    • 1997
  • 단백질 결합 다당류인 PS-K가 Edwardsiella tarda의 감염에 대한 나일 틸라피아(Oreochromis niloticus)의 저항력 증강 효과에 대한 능력분석을 실시하였다. PS-K를 0.1 mg/g 어체중의 농도로 처리한 나일 틸라피아에 대하여 여러 농도외 E. tarda로 공격 실험을 하였다. 저농도($1.2{\times}10^7$ 그리고 $1.2{\times}10^8$ cfu/틸라피아)의 공격에 대하여 PS-K 처리구는 100% 생존율을 나타내었고 대조구는 각각 50%와 100%의 폐사율을 보여 주었다. 그러나 고농도의 E. tarda($1.2{\times}10^9$ 그리고 $1.2{\times}10^{10}$ cfu/틸라피아) 공격에 대해서는 약간의 수명 연장효과만 있었을뿐 대조구와 같이 100%의 폐사율을 보여 주었다. E. tarda($1.2{\times}10^7$ cfu/틸라피아) 공격 1주일 후 PS-K를 복강 투여한 나일 틸라피아는 100%의 생존율을 나타내었으나 대조구는 50% 생존율을 나타내어 PS-K가 가지고 있는 치료제로서의 잠재력을 보여 주었다. 그리고 PS-K 투여 1주일 후 주사된 E. tarda FKC에 대하여 보여준 나일 틸라피아의 높은 항체 반응은 PS-K가 어류의 특이 액성 면역반응에 관여하여 어류가 세균 감염시 높은 저항성을 갖게 하여 주는 것으로 나타났다.

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2021년부터 2023년까지 뱀장어(Anguilla japonica) 질병 모니터링: 혈관내피세포감염바이러스(JEECV)와 Edwardsiella anguillarum의 중요성 (Monitoring of Japanese eel (Anguilla japonica) diseases from 2021 to 2023: significance of Japanese Eel Endothelial Cells-infecting Virus (JEECV) and Edwardsiella anguillarum)

  • 김현경;장문희;정성주
    • 한국어병학회지
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    • 제36권2호
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    • pp.239-250
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    • 2023
  • 최근 뱀장어 (Anguilla japonica)에서 발생하고 있는 질병을 파악하기 위하여 질병 모니터링을 실시하였다. 2021년 5월부터 2022년 3월까지는 폐사가 발생한 7개의 양만장 유래의 뱀장어를 7회 검사하였다. 모든 양만장에서 JEECV가 검출되었고 양만장마다 1-2종의 세균 (Edwardsiella anguillarum, E. piscisida, Aeromonas sp., Citrobacter freundii, Lactococcus garviae 혹은 Vibrio sp.)이 복합감염이 일어나 있었다. 2022년 3월부터 2023년 10월까지는 전라남도 영광의 한 양식장에서 매달 정기적으로 총 22회 검사를 실시하였다. JEECV가 22회 검사 중 10회 검출되었고 폐사가 발생하지 않는 시기에도 꾸준히 검출되었다. 세균으로는 E. anguillarum, C. freundii, Aeromonas sp.와 Vibrio sp가검출되었으나 E. anguillarum이 분리된 개체만이 간의 농양과 출혈 병소가 일관되게 관찰되었고 다른 세균은 임상증상이 없는 경우가 많았다. 모니터링의 결과를 종합하면 양만장에서의 폐사는 JEECV 단독감염보다는 JEECV가 세균과 복합감염일 경우에 발생하였다. 그러므로 뱀장어의 폐사를 줄이기 위해서는 JEECV와 E. anguillarum의 관리에 중점을 두고 복합감염을 줄이는 것이 중요한 것으로 생각된다.

가물치, Channa arga 자어에서 분리한 Rhabdovirus 유사 병원체 (Isolation of rhabdovirus-like from fry of the fry of the snakehead fish, Channa arga)

  • 김수미;홍미주;박수일
    • 한국어병학회지
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    • 제19권1호
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    • pp.55-64
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    • 2006
  • 2003~2004년 봄, 대량 폐사한 가물치 자어 (부화 후 15~30일령)에서 랩도바이러스 유사 병원체를 분리하였다. 분리 바이러스주는 EPC cell과 SSN-1 cell에서 잘 증식하는 반면 FHM cell에는 감수성이 없었고 감염된 EPC cell에서 전형적인 랩도바이러스 모양의 탄환형 바이러스 입자 (45×100 nm)를 다수 관찰할 수 있었다. 분리 바이러스주의 증식 적온은 20~25℃이었고, 15℃이하에서는 증식할 수 없었으며, 30℃ 이상에서는 증식하지는 않았으나 초기 감염가를 유지하였다. 분리 바이러스는 15일령 및 40일령의 가물치 자치어에서는 침지 감염에서 병원성이 높은 반면, 90일령 이상의 중간 육성어에서는 폐사를 나타내지는 않았다.

Molecular and Histopathological Evidence of Mycobacteriosis in Paradise Fish Macropodus opercularis Imported into Korea

  • Han, Hyun-Ja;Kim, Jeong-Ho;Jeon, Chan-Hyeok;Kim, Wi-Sik;Kim, Do-Hyung;Jung, Sung-Ju;Oh, Myung-Joo
    • Fisheries and Aquatic Sciences
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    • 제16권3호
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    • pp.165-169
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    • 2013
  • We report on mycobacteriosis in an imported tropical ornamental fish Macropodus opercularis commonly known as the paradise fish. Mass mortality occurred in paradise fish imported to Korea from Southeast Asia in 2008. The affected fish did not show any outward clinical signs, but enlargement of the spleen, kidneys, and liver was observed on dissection. Histopathological examination revealed numerous granulomas in the spleen, and acid-fast bacilli were observed in the centers of the granulomas. About 65% of spleen DNA samples were PCR positive using mycobacteria-specific primers targeting the 16S rRNA and hsp65 genes. The nucleotide identities of the 16S rRNA and hsp65 genes with those of Mycobacterium marinum were 99.5% and 99.4%, respectively. Although the bacterium was not cultured, the molecular diagnosis and histopathological findings were consistent with mycobacteriosis in paradise fish.

스마트 양식장 수조 내 용존 산소 및 온도 제어를 위한 시스템 구현 (An Implementation of System for Control of Dissolved Oxygen and Temperature in the pools of Smart Fish Farm)

  • 전주현;이윤호;이나은;주문갑
    • 대한임베디드공학회논문지
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    • 제16권6호
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    • pp.299-305
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    • 2021
  • Dissolved oxygen, pH, and temperature are the most important factors for fish farming because they affect fish growth and mass mortality of the fish. Therefore, fish farm workers must always check all pools on the farm, but this is very difficult in reality. That's why we developed a control system for smart fish farms. This system includes a gateway, sensor gatherers, and a PC program using LabVIEW. One sensor gatherer can cover up to four pools. The sensor gatherers are connected to the gateway in the form of a bus. For the gateway, the ATmega2560 is used as the main processor for communication and the STM32F429 is used as a sub-processor for displaying LCD. For the sensor gatherer, ATmega2560 is used as the main processor for communication. MQTT (Message Queuing Telemetry Transport), RS-485, and Zigbee are used as the communication protocols in the control system. The users can control the temperature and the dissolved oxygen using the PC program. The commands are transferred from the PC program to the gateway through the MQTT protocol. When the gateway gets the commands, it transfers the commands to the appropriate sensor gatherer through RS-485 and Zigbee.

Ichthyotoxicity of a harmful dinoflagellate Cochlodinium polykrikoides: Aspect of biochemical and hematological responses of fish exposed to Algal blooms

  • Kim, Chang-Sook;Bae, Heon-Meen;Cho, Yong-Chul
    • 한국어업기술학회:학술대회논문집
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    • 한국어업기술학회 2000년도 추계수산관련학회 공동학술대회발표요지집
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    • pp.141-142
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    • 2000
  • To elucidate the ichthyotoxic mechanisms of a harmful dinoflagellate Cochlodinium polykrikoides, biochemical and hematological responses of fish exposed to blooms were investigated. Particularly, based on our finding that oxidative damages of gill were associated with fish mortality, dysfunction of ion-transporting enzymes and secretion of gill mucus of fish exposed to this bloom species were examined. (omitted)

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Iridovirus의 해산 양식어류에 대한 병원성과 사육수에서의 검출 (Pathogenicity of Iridovirus against Marine Fish and Its Detection in Culturing Seawater)

  • 정준범;정현도
    • 한국수산과학회지
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    • 제41권1호
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    • pp.20-25
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    • 2008
  • The susceptibility of five different marine fish to iridovirus IVS-1 infection was analyzed and found a higher the cumulative mortality in the order of rock bream (Oplegnathus fasciatus), red sea bream (Pagrus major), sea perch (Lateolabrax sp.), rockfish (Sebastes schlegeli) and black porgy (Acanthopagrus schlegeli). However, the concentrations of virus in the infected spleens of these species did not differ significantly. To determine the release of iridovirus from infected fish into culturing seawater, rock bream were challenged with iridovirus IVS-1 and the concentration of virus in the water was analyzed using PCR. Over the 10 days of the analysis, the linear relationship between the number of dead fish and viral DNA concentration found in culturing seawater should be considered direct evidence of horizontal iridovirus transmission.