• Journal of the Korean Association of Geographic Information Studies
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• v.14 no.2
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• pp.147-161
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• 2011

Korea is surrounded by sea on three sides. This country has been supplied with a variety of aquaculture products cultivated on shores. There have recently been a lot of studies to have better understanding of the correct location and quantity of aquaculture farms for seaweed, ear shells and fish that cover a wide area of sea. And it is necessary to use the geographic information system and remote sensing to detect the aquaculture farms in order to effectively manage them. This study uses higher resolution aerial images(25 centimeters) than satellite images of 2~2.5-meter resolution that have been ever used, to conduct an accuracy detection of aquaculture farming facilities. It chooses as the case study area the Wando region that has aquaculture farms for seaweed, ear shells and fish. Aerial photos of the island were obtained in this study and an image correction of them was conducted. A spatial database was then constructed in this study and the detection of aquaculture farming facilities was performed. An analysis of facilities inside and outside the permitted areas reveals that there has been an increase in the facilities of seaweed and ear shell aquaculture farms outside the permitted areas. And also it tells that because the facilities of fish aquaculture farms have turned into those of ear shell aquaculture farms, there has been a decrease in permitted facilities, facilities detected on the basis of aerial images, and facilities outside the permitted area. It will be necessary to continuously control and manage the unpermitted facilities, regarding the increase in the facilities inside and outside the permitted area for seaweed and ear shell aquaculture farms. Because the facilities of aquaculture farms cover a wide range of areas(sea) in this manner, it is more effective to depend on high resolution aerial images than a field survey to detect and calculate the facilities. This study comes up with a plan for using aerial images to detect the location and the quantity of the fish aquaculture facilities and then effectively manage them.

• Fisheries and Aquatic Sciences
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• v.7 no.2
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• pp.64-69
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• 2004

A bacteriological survey for 20 large grocery stores (M 1 to M20) in Korea was investigated for one year. The average detection rate of Esherichia coli was $22\%$ (166/763) for 7 kinds of ready-to-eat food through the year, where each grocery store and each type of food showed different detection rates. Eleven grocery stores showed lower detection rates, while 9 grocery stores showed a higher than average rate. Especially, M3 showed a rate that was twice as high as the average and one which was 7 times higher than M14, which had the lowest rate of $6\%$ E. coli detection. The detection rate for each type of food was: $38\%$ (41/109) for Kimbop, $31\%$ (34/109) for vegetable salad, $19\%$ (21/109) for bean-curd, $18\%$ (20/109) for the cooked materials used in making Kimbop, $17\%$ (19/109) for Hoe (sliced raw fish) and Sushi (Japanese vinegared rice delicacies), and $11\%$ (12/109) for cooked pork hock. During the summer, the E. coli detection rate averaged $43\%$ (71/166), which was twice as high as other seasons. Most (89/100) of the food contacting surfaces contained more than the critical limit $(1.3\;log_{10}\;CFU/10cm^2)$ of aerobic viable cell counts (AVC). The $log_{10}$ AVC and $log_{10}$ coliform count (CC) of 218 meat samples (beef, pork, and chicken) ranged between 4.6-7.1 CFU/g and 1.9-6.4 CFU/g, except for 41 meat samples $(19\%)$ which were found to contain no coliform. There was a definite correlation between the $log_{10}$ AVC and $log_{10}$ CC, and the values of $log_{10}$ CC made a more accurate straight than the $log_{10}$ AVC, which are variable. From these results, it is suggested that a detection rating of less than 2.1 CFU/g of $log_{10}$ CC (correspond to 5.0 CFU/g of $log_{10}$ AVC) is the critical point of freshness, and a rating of more than 6.3 CFU/g of $log_{10}$ CC (correspond to 7.0 CFU/g of $log_{10}$ AVC) can be considered an initial spoilage point.

• Journal of Food Hygiene and Safety
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• v.31 no.5
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• pp.319-326
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• 2016

Colistin is a last resort antimicrobial agent against multi-drug resistant Gram-negative bacteria. This study was conducted to develop an analytical method to determine colistin in fish and shrimp. The analytes were confirmed and quantified via liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the positive ion mode using multiple reaction monitoring (MRM). The sample was extracted with acidified 5% methanol (containing 0.5% formic acid). Then, solid phase extraction (SPE) was used for cleanup. Matrix-matched calibration curves were linear over the calibration ranges (0.05-1.2 mg/kg) for all the analytes into blank sample with $r^2$ > 0.99. All the values fulfilled the criteria requested by the Codex guidelines. Average recoveries ranged from 85.9% to 107.9%. The repeatability of measurements, expressed as the coefficient of variation (CV, %), was less than 15%. The limit of detection (LOD) was 0.02 mg/kg, and the limit of quantitation (LOQ) was 0.05 mg/kg. This improved method showed higher accuracy and acceptable sensitivity to meet the CAC guideline requirements and is applicable for the analysis of residual colistin (A+B) in fish and shrimp.

• Clinical and Experimental Pediatrics
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• v.56 no.6
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• pp.247-253
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• 2013

Purpose: In children with acute leukemia, bone marrow genetic abnormalities (GA) have prognostic significance, and may be the basis for minimal residual disease monitoring. Since April 2007, we have used a multiplex reverse transcriptase-polymerase chain reaction tool (HemaVision) to detect of GA. Methods: In this study, we reviewed the results of HemaVision screening in 270 children with acute leukemia, newly diagnosed at The Catholic University of Korea from April 2007 to December 2011, and compared the results with those of fluorescence in situ hybridization (FISH), and G-band karyotyping. Results: Among the 270 children (153 males, 117 females), 187 acute lymphoblastic leukemia and 74 acute myeloid leukemia patients were identified. Overall, GA was detected in 230 patients (85.2%). HemaVision, FISH, and G-band karyotyping identified GA in 125 (46.3%), 126 (46.7%), and 215 patients (79.6%), respectively. TEL-AML1 (20.9%, 39/187) and AML1-ETO (27%, 20/74) were the most common GA in ALL and AML, respectively. Overall sensitivity of HemaVision was 98.4%, with false-negative results in 2 instances: 1 each for TEL-AML1 and MLL-AF4. An aggregate of diseases-specific FISH showed 100% sensitivity in detection of GA covered by HemaVision for actual probes utilized. G-band karyotype revealed GA other than those covered by HemaVison screening in 133 patients (49.3%). Except for hyperdiplody and hypodiploidy, recurrent GA as defined by the World Health Organizationthat were not screened by HemaVision, were absent in the karyotype. Conclusion: HemaVision, supported by an aggregate of FISH tests for important translocations, may allow for accurate diagnosis of GA in Korean children with acute leukemia.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.40 no.1
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• pp.78-85
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• 2004

The new biotelemetry method and system that the installation and the treatment of equipment is convenient and the instantaneously detailed position of the fish attached a pinger is able to track comparatively easily had been developed, an practicality of it were verified in the water tank and the small sea port through the tracking of fish. The biotelemetry method had been gotten the three dimensional locations of fish to the receiving transducer by combining of the super short base line (SSBL) method to detect the direction of pinger and the pinger synchronizing method to measure the range from receiving transducer to pinger. The receiving system had been designed to realize the high precision or wide detection range by application of the basic design method for receiving system of biotelemetry and the hydrophone array configuration. From tracking test of carp in the water tank, the migration course and the velocity of carp was investigated and the observed migration course was compared with measurement. The measured migration course of carp coincided with the observation in the main and the position of carp was able to track three dimensionally. The velocity of carp measured by the moving average method was 11.2cm/s. From tracking test of yellowtail in the small sea port, the migration course and the velocity of yellowtail was investigated at natural condition. The position of yellowtail was able to track three dimensionally and the velocity of it measured by moving average method was 43.9cm/s.

• Journal of fish pathology
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• v.30 no.1
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• pp.1-9
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• 2017

This study was conducted to monitor the prevalence of viral hemorrhagic septicemia virus (VHSV), nervous necrosis virus (NNV) and marine birnavirus (MABV) in cultured walleye pollock Gadus chalcogrammus by RT-PCR. All of the viruses tested were not detected by one-step PCR in 62 spleen sample sets, except for NNV in one brain sample set (1/55). By two-step PCR, VHSV was detected in 51.6%(32/62) and NNV was detected in 1.6%(1/62) spleen sample set, but MABV was not detected. In the brain sample sets, the detection rate of NNV was 3.6%(2/55). VHSV and NNV were detected for the first time in cultured walleye pollock in this study. However, the titers of viruses in these sample sets are thought to be very low, because most of the positive sample sets were detected by two-step PCR and none of the fish showed any clinical symptoms of each virus. Continuous monitoring, subsequent virus isolation and validation of carrier fish will be necessary.

• Journal of fish pathology
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• v.11 no.1
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• pp.61-67
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• 1998

For rapid detection of iridovirus infection, a PCR-based diagnostic method was developed. The genomic DNA from mortality-associated iridovirus was cloned into pUC19 vector. The nucleotide sequences of these clones were compared with sequences of other genes from EMBL/GenBank databank. Based on the nucleotide sequences, PCR primers were prepared and used for PCR. The DNA amplification did not occur from the normal fish cells. In contrast, DNA was amplified from the iridovirus-infected fish cells and purified iridovirus. These results suggest that mortality-associated iridovirus can be detected from virus-infected cells within short time and this PCR-based diagnostic system provides a simple and accurate method for detecting the presence of iridovirus infection.

• Korean Journal of Environmental Agriculture
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• v.28 no.2
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• pp.209-220
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• 2009

Octachlorostyrene(OCS) has been persisted in environment because it has not been decomposed easily. And, it has been known as highly toxic compounds to the environment and human as well as accumulated as high concentrations in a biota through a food chain. Therefore, OCS was monitored for water, soil and fish sampled from the areas where were able to be contaminated with OCS. The recoveries of octachlorostyrene were 93.1${\sim}$98.6% in water, 90.4${\sim}$94.8% in soil and 81.5${\sim}$90.2% in fish and detection limits were 0.0004 mg $L^{-1}$ for water, and 0.002 mg $kg^{-1}$ for soil and fish, respectively. OCS was not detected in water, sediment, soil and fish samples from Ulsan, Yeosu, Daejeon and Sihwa industrial complex and in soil sampled nearby incineration plants in all parts of the country. Accordingly, we estimated that there is no risk from exposure of OCS.

• Journal of Life Science
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• v.23 no.12
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• pp.1557-1561
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• 2013

Carbonic anhydrase isozymes are a widespread, zinc-containing metalloenzyme family. The enzyme catalyzes the reversible inter-conversion of $CO_2$ and $HCO_3$. This reaction is the main role played by CA enzymes in physiological conditions. This enzyme has been found in virtually all organisms, and at least 16 isozymes have been isolated in mammals. Unlike mammals, there is little information available regarding CA isozymes in the tissues of non-mammalian groups, such as fish. Carbonic anhydrase is very important in the osmotic and acid-base regulation in fish. It is well-known that the gills of fish play the most important role in acid-base relevant ion transfer, the transfer of $H^+$ and/or $HCO_3^-$, for the maintenance of systemic pH. Rainbow trout, Oncorhynchus mykiss, is the most important freshwater fish species in the aquaculture industry of Korea, with annual production increasing each year. In addition, environmental toxicology research has shown that rainbow trout is known to be the species that is most susceptible to environmental toxins. Consequently, carbonic anhydrase was detected in rainbow trout, Oncorhynchus mykiss. The isolated protein showed the specific band with a molecular weight of 30 kDa and pI of 7.0, and it was identified as being carbonic anhydrase. The immunohistochemical result demonstrated that the carbonic anhydrase was located in the epithelial cells of the gills.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.1
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• pp.13-18
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• 2019

We determined the contents of 11 biogenic amines in 20 types of cabbage kimchi, 10 types of fish sauce, and 40 types of salted fish purchased from small- and medium-sized grocery stores in Gyeonggi-Do, Republic of Korea, from June to October 2017. The content of each of the biogenic amines in the various types of cabbage kimchi was slightly lower than that in detection amounts of each of the 10 types of biogenic amines and the values reported by Joe et al. The histamine content of all five types of anchovy sauce was lower than the limit set by the European Union (400 mg/kg), but that of three of the five types of sand lance sauce was higher. Analyzing the ratios of the contents of five highly toxic biogenic amines to those of the 11 biogenic amines yielded no quantitative correlation. The estimated daily intake of the five highly toxic biogenic amines in cabbage-kimchi and fermented fish was ${\leq}6mg$.